Article
Chemistry, Analytical
Da-Qian Feng, Guoliang Liu
Summary: The research demonstrates that the emission wavelength and intensity of silver nanoclusters (Ag NCs) can be effectively regulated through configuration transformation from adsorption to desorption. By conjugating complementary sequences as capturing probes, targets can be activated to emit fluorescent signals.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Siqi Huang, Caijun Wu, Yuying Wang, Xia Yang, Ruo Yuan, Yaqin Chai
Summary: The Ag/TiO2 nanocomposites were prepared via catalytic hairpin assembly (CHA) strategy to fabricate a SERS biosensor for sensitive detection of UDG, with the introduction of Pb2+ assisted DNAzyme cleavage reaction enhancing the sensitivity of the method, achieving a detection limit of 1.2×10-5 U mL-1.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Fang Yang, Yan-Wei He, Ya-Qin Chai, Ruo Yuan, Ying Zhuo
Summary: A high-efficient DNA amplifier was developed by localizing CPs on Au nanoparticle modified magnetic beads, enabling efficient capture and recycling of target for detection of trace K-ras gene. Additionally, a novel ECL indicator Pe@Ag MFs showed desirable performance by reducing inner filter effect and enhancing ECL intensity through reaction with Ag as coreaction accelerator. This work provides a new paradigm for efficient amplification strategy and high-efficiency ECL indicators preparation.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Da-Qian Feng, Guoliang Liu
Summary: A new multicolor silver nanoclusters/graphene oxide hybrid material has been demonstrated to achieve emission shift and enhancement through entropy-driven enzyme-free toehold-mediated strand displacement reaction. By designing two different synthesis routes, the material successfully constructs a biosensor for influenza A virus subtype genes, resulting in significant fluorescence enhancement and emission shift. This novel approach shows promising application prospect in accurate diagnosis and treatment with remarkable emission recovery rates and multichannel signal.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Jeong Ah Park, Jinmyeong Kim, Soo Min Kim, Hiesang Sohn, Chulhwan Park, Tae-Hyung Kim, Jin-Ho Lee, Min-Ho Lee, Taek Lee
Summary: This study proposed an electrochemical biosensor using a multifunctional DNA four-way junction (4WJ) and carboxyl molybdenum disulfide (carboxyl-MoS2) hybrid material for precise detection of H1N1, showing excellent specificity and selectivity.
Article
Engineering, Environmental
Zhiwei Sun, Juan Li, Yao Tong, Yufei Yang, Hui Li, Chuanxin Wang, Lutao Du, Yanyan Jiang
Summary: This article presents a ratiometric fluorescent biosensing strategy for the precise detection of colorectal cancer-associated miR-92a-3p. The strategy combines a nanoplatform composed of DNA-templated Ag-Au nanoclusters and Au nanoparticles/g-C3N4 nanosheets with catalytic hairpin assembly and sequence-induced fluorescence enhancement. It demonstrates a low detection limit, excellent selectivity, and the ability to detect miR-92a-3p in clinical exosome samples.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Virology
Denisa Bojkova, Marco Bechtel, Tamara Rothenburger, Joshua D. D. Kandler, Lauren Hayes, Ruth Olmer, Ulrich Martin, Danny Jonigk, Sandra Ciesek, Mark N. N. Wass, Martin Michaelis, Jindrich Cinatl Jr
Summary: Recent findings show that SARS-CoV-2 Omicron BA.1 induces a stronger interferon response compared to Delta. In this study, it was demonstrated that BA.1 and BA.5, but not Delta, induce an antiviral state in air-liquid interface cultures of primary human bronchial epithelial cells and primary human monocytes. Both Omicron subvariants trigger the production of biologically active interferons and protect cells from super-infection with influenza A viruses. These findings highlight the significance of Omicron in the immune response against viral infections.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Yu Xiong, Jianyuan Dai, Yujiao Zhang, Cuisong Zhou, Hongyan Yuan, Dan Xiao
Summary: This study reported a label-free fluorescent signal amplification system based on CHA, which was successfully used for sensitive detection of HIV DNA and Pb2+. By triggering the CHA reaction to release G-quadruplex sequences and bind ThT dye to provide fluorescence signals, and continuing the CHA cycle to amplify the signal.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Physical
Chittatosh Pal, Subrata Majumder
Summary: This paper proposes a sensitive, selective, and facile electrochemical biosensor based on non-covalent ssDNA-MWNT hybridization for the detection of Ag+ ions in an aqueous medium. The biosensor achieved selective detection of Ag+ metal ions with a lowest concentration of 1 pM in the aqueous medium, and showed potential for trace amount determination in real sample studies for environmental monitoring purposes.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Analytical
Zihui Liang, Yixin Nie, Xin Zhang, Peilin Wang, Qiang Ma
Summary: A novel multiplex electrochemiluminescence polarization assay was developed to detect breast cancer-related genes BRCA1 and BRCA2 simultaneously. The study revealed the relation between the structure of metal nanomaterials and SPC-ECL, and a high polarization-resolved sensing system was designed to detect multitarget DNA. Polarization-based multiple ECL analysis has broad prospects in related cancer diagnosis and treatment evaluation.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Changpeng Hu, Rabia Tahir, Hanbin Mao
Summary: Single-molecule mechanochemical sensing is a novel biosensing technique that uses mechanical force as a signal transduction mechanism. By converting the chemical binding of analyte molecules to mechanical signals, this technique allows for sensitive sensing at the single molecule level. Compared to traditional ensemble sensing methods, single-molecule mechanochemical sensing offers higher material efficiency and temporal resolution.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Engineering, Environmental
Bo Shen, Qian Wu, Yunpeng Fan, Haiping Wu, Xinmin Li, Xiaofen Zhao, Yuwei Wang, Shijia Ding, Juan Zhang
Summary: An efficient electrochemiluminescent biosensor was developed for ultrasensitive detection of miRNA in cancer diagnosis. The biosensor utilized DNA nanoframework-carried emitters and T7 exonuclease-assisted target recycling amplification. The biosensor exhibited a broad linear range and low detection limit, and was successfully applied to miRNA analysis in breast cancer cells.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Dinesh Babu Manikandan, Manikandan Arumugam, Arun Sridhar, Balaji Perumalsamy, Thirumurugan Ramasamy
Summary: In this study, sustainable fabrication of silver-copper oxide nanocomposites (Ag-CuO NCs) was achieved using Ocimum americanum L. through one-pot green chemistry method. The Ag-CuO NCs exhibited excellent antibacterial, anticancer, dye degradation, and microalgae growth inhibition activities. The surface properties and stability of the NCs were characterized using various techniques. The findings of this study highlight the potential of Ag-CuO NCs for various environmental applications.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biophysics
Qianqian Cai, Di Wu, Hongkun Li, Guifen Jie, Hong Zhou
Summary: A novel 3D CdSe QDs-DNA nanonetwork was assembled to sensitize the SnO2 substrate, coupled with a biped DNA walker multiple amplification method for dual detection of HIV. Introduction of the target-triggered DNA walker amplification greatly amplified the sensitive HIV detection signals.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).