Article
Chemistry, Analytical
Hu Jiang, Hu Su, Kesheng Wu, Zemin Dong, Xiangmin Li, Lijuan Nie, Yuankui Leng, Yonghua Xiong
Summary: A multiplexed competitive lateral flow immunoassay based on inner filter effect was developed for simultaneous detection of three mycotoxins in maize. The method exhibited high sensitivity and accuracy, and allowed quantitative detection in diluted maize extract solution.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
William Teixeira, Yeray Pallas-Tamarit, Augusto Juste-Dolz, Amadeo Sena-Torralba, Roberto Gozalbo-Rovira, Jesus Rodriguez-Diaz, David Navarro, Javier Carrascosa, David Gimenez-Romero, Angel Maquieira, Sergi Morais
Summary: The COVID-19 pandemic has increased the demand for rapid and reliable point-of-care testing devices. This study presents an all-in-one POCT device that can perform parallel viral antigen and serological analysis. The biosensor demonstrates high diagnostic sensitivity and specificity for various respiratory viruses.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Huibing Li, Xianming Liu, Fengjiao Zhu, Dachuan Ma, Chunyue Miao, Haoran Su, Jiu Deng, Haiyue Ye, Hongyu Dong, Xue Bai, Yong Luo, Bingcheng Lin, Tingjiao Liu, Yao Lu
Summary: A digital microfluidics (DMF) platform with spatial barcoding was developed to achieve highly multiplexed immunoassay. The platform showed excellent sensitivity and reproducibility in evaluating protein abundance from biological samples. This study opens up new opportunities for multiplexity-associated applications with the DMF platform.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Alyse D. Krausz, Frederick K. Korley, Mark A. Burns
Summary: The study introduced a novel variable height microfluidic device for multiplexing multiple TBI biomarkers simultaneously, which can passively multiplex bead-based immunoassays by trapping beads based on their diameter matching the channel height.
Article
Chemistry, Multidisciplinary
Weiqi Zhao, Yang Zhou, Yao-Ze Feng, Xiaohu Niu, Yongkun Zhao, Junpeng Zhao, Yongzhen Dong, Mingqian Tan, Yunlei Xianyu, Yiping Chen
Summary: In this work, a multidimensional digital immunoassay has been developed using microparticle-based encoding and artificial intelligence-based decoding, enabling multiplexed detection with high sensitivity and convenient operation. The features of microspheres, including size, number, and color, encode information for simultaneous identification and accurate quantification of multiple targets. Computer vision-based artificial intelligence analyzes microscopy images for information decoding and visually outputs identification results. Additionally, optical microscopy imaging can be integrated with microfluidic platforms for encoding-decoding using computer vision-based artificial intelligence. This microfluidic digital immunoassay can simultaneously analyze multiple inflammatory markers and antibiotics within 30 minutes, with a broad detection range from pg/mL to μg/mL, holding great promise as an intelligent bioassay for next-generation multiplexed biosensing.
Article
Biophysics
Sifan Meng, Runzhi Chen, Jingxuan Xie, Jing Li, Jiayi Cheng, Yanan Xu, Haiting Cao, Xiaofeng Wu, Qiang Zhang, Houyu Wang
Summary: This study developed an innovative 3D surface-enhanced Raman scattering holography sensing strategy for rapid, sensitive, and multiplexed detection of human breast cancer-associated miRNAs. By spatially separating amplified miRNAs on silicon SERS substrates and using different Raman reporters as SERS tags, the detection of miRNAs was completed quickly and with high sensitivity.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Physical
Yingwen Chen, Xuanqun Wang, Xing Na, Yingkun Zhang, Zan Li, Xiaohui Chen, Linfeng Cai, Jia Song, Ren Xu, Chaoyong Yang
Summary: Single-cell microRNA (miRNA) sequencing provides comprehensive insights into the abundance and complex networks of miRNAs and their dynamic regulation of cellular events. However, current benchtop-based technologies for single-cell miRNA sequencing have limitations in terms of throughput, efficiency, manual operations, and reagent costs. In this study, a highly multiplexed and automated sample preparation platform called Hiper-seq is introduced, which is based on digital microfluidics (DMF). The platform allows for the construction of miRNA sequencing libraries through digital control of addressable droplets on the DMF chip, enabling selective isolation and parallel processing of multiple single cells. This platform offers higher throughput and efficiency for single-cell miRNA measurement, with a smaller reaction volume and lower reagent cost compared to benchtop methods. Furthermore, it has been successfully applied to explore miRNAs involved in the ossification of mouse skeletal stem cells after bone fracture, leading to the discovery of previously unreported miRNAs that regulate bone repairing.
Article
Biophysics
Shih-Mo Yang, Qiang Lin, Hongbo Zhang, Ruixue Yin, Wenjun Zhang, Minchao Zhang, Yubao Cui
Summary: The study introduced a method utilizing electrokinetic effects to improve the efficiency of cytokine detection, resulting in an increased fill percentage of encoded beads and successful calculation of the concentration of four target cytokines to the pg/ml level. The application of DEP is expected to enhance the sensitivity of digital ELISA for rapid detection of multiple targets.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Food Science & Technology
Xiaoying Han, Yuanhui Liu, Juxin Yin, Min Yue, Ying Mu
Summary: The global burden of foodborne diseases is significant, with foodborne pathogens being the major cause of human illnesses. Microfluidic technology enables rapid, cost-effective, sensitive, and portable pathogen detection to meet on-site testing requirements. Multiplexed detection technology offers a new opportunity to ensure food safety by simultaneously detecting multiple pathogens.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Chang Liu, Yu Qie, Weiping Qin, Keda Zhao, Jianqiao Zhu, Lixia Zhao, Minjie Li, Liang-Hong Guo
Summary: Exosomes are nano-scale biofilm-enclosed vesicles involved in intercellular communications and seen as potential biomarkers for liquid biopsy. Traditional exosome detection methods have limited sensitivity and specificity, prompting the need for new analytical approaches. Immunoassay-based technologies for rapid exosome detection have gained significant interest recently, offering new possibilities for early disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
P. Nolan, S. Auer, A. Spehar, M. Oplatowska-Stachowiak, K. Campbell
Summary: To enhance global food security, there is a demand for improved monitoring of various toxins throughout the food supply chain. This study investigated the feasibility of using mass-based biosensors for multiplex mycotoxin detection, demonstrating the ability to simultaneously and semi-quantitatively detect three regulated toxins.
Review
Chemistry, Analytical
Kieu The Loan Trinh, Hoang Dang Khoa Do, Nae Yoon Lee
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has triggered a demand for accurate and cost-effective diagnostic tests for COVID-19 and other viral infections. Traditional virus screening methods have limitations in terms of equipment, personnel, and analysis time. Microfluidic-based biosensors, integrating microfluidic and biosensor technologies, offer a platform for sample preparation and simultaneous detection with high sensitivity, accuracy, portability, and real-time results. This review presents recent advances in microfluidic-based biosensors for virus detection, categorizing them into immunoassays and molecular assays, and analyzes available commercial sensing tests.
Article
Chemistry, Analytical
Yu-Long Li, Fa-Ting Xie, Cao Yao, Gui-Qun Zhang, Yan Guan, Yun-Hui Yang, Jian-Mei Yang, Rong Hu
Summary: In this study, a ratiometric electrochemical aptasensor based on a DNA tetrahedral nanomaterial and a zirconium metal-organic framework was developed for the detection of Ochratoxin A (OTA). The sensor exhibited high specificity for OTA and a good linear range, allowing for the effective detection of OTA in corn kernel samples.
Review
Engineering, Environmental
Saowalak Adunphatcharaphon, Christopher T. Elliott, Thanasat Sooksimuang, Ratthaphol Charlermroj, Awanwee Petchkongkaew, Nitsara Karoonuthaisiri
Summary: Mycotoxins pose serious threats to public health, the economy, and the environment. The co-contamination of multiple hazardous mycotoxins in commodities and the environment adds complexity to the problem. This review discusses emerging rapid immuno-based multiplex methods for detecting mycotoxins in agricultural products and feed ingredients published within the past five years, including lateral flow, fluorescence polarization, chemiluminescence, surface plasmon resonance, surface enhanced Raman scattering, electrochemical sensor, and nanoarray. The future trend of detection methods for multiple mycotoxins is predicted to rely on advancements in sensor technologies, nanomaterial-based enhancing and reporting signals, rapid sample preparation, and quantitative analysis capacity.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Chemistry, Applied
Ping Li, Shengliang Deng, Zhenjiang Zech Xu
Summary: Substitute-based immunoassay technologies offer an effective solution to the recurring mycotoxins contamination in food, providing high sensitivity and renewability, while posing minimal threats to operators and the environment.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).