Article
Immunology
Ana Raquel Maceiras, Diogo Silverio, Rute Goncalves, Marcos S. Cardoso, Margarida Saraiva
Summary: During infection, bone marrow hematopoiesis is reprogrammed to produce myeloid cells, a mechanism called emergency myelopoiesis. However, studies using the mouse model of infection and laboratory reference strain M. tuberculosis H37Rv have shown limited emergency myelopoiesis and trained immunity. This study used a hypervirulent M. tuberculosis isolate HN878 to infect mice and found increased frequencies of LSK cells and the GMP population, as well as an increase in monocytes and neutrophils in the blood and lung. However, there was no trained immunity observed in the bone marrow.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Alexander Belyavsky, Nataliya Petinati, Nina Drize
Summary: In the bone marrow of vertebrates, hematopoiesis relies on the interaction of hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs). HSC descendants produce mature blood cells, while MSCs form a niche for HSCs and regulate the hematopoietic process. Various research methods have provided new insights into hematopoiesis, its development in embryos, and changes during aging in mammals and humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Camila Medrano-Trochez, Paramita Chatterjee, Pallab Pradhan, Hazel Y. Stevens, Molly E. Ogle, Edward A. Botchwey, Joanne Kurtzberg, Carolyn Yeago, Greg Gibson, Krishnendu Roy
Summary: This study showed that donor differences in hMSC transcriptome are minor compared to the intrinsic differences in tissue-of-origin. hMSCs with different transcriptomic profiles exhibited potential differences in functional characteristics, contributing to the understanding of differences in out-of-thaw therapeutic hMSC products.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Immunology
Matthew D. Patrick, Ramkumar T. Annamalai
Summary: This study enhances the immunomodulatory potential of mesenchymal stromal cells (MSC) by priming them with IFN gamma and TNF alpha, and develops gelatin-based microgels. These microgels possess adsorption capacity and sustained release effect, showing great potential in immunomodulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Mario Barilani, Christopher Lovejoy, Roberta Piras, Andrey Y. Abramov, Lorenza Lazzari, Plamena R. Angelova
Summary: The study found that aging in MSCs is associated with changes in mitochondrial metabolism, including a decrease in mitochondrial membrane potential, lower NADH levels, and higher mitochondrial DNA content. Despite higher ATP levels in aged cells, increased rates of mitophagy lead to decreased overall mitochondrial mass. Additionally, changes in mitochondrial metabolism in aged MSCs result in the overproduction of reactive oxygen species in mitochondria, which is compensated by higher levels of the endogenous antioxidant glutathione.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Orthopedics
Ava Brozovich, Brent J. Sinicrope, Guillermo Bauza, Federica Banche Niclot, David Lintner, Francesca Taraballi, Patrick C. McCulloch
Summary: This study aimed to characterize bone marrow aspirate (BMA) versus bone marrow aspirate concentrate (BMAC) for mesenchymal stem cell (MSC) quantity, potency, and cytokine profile. The results showed significantly higher MSC concentration and CFU number in BMA compared to BMAC, with BMAC failing to concentrate MSC in 6 out of 8 samples.
ORTHOPAEDIC JOURNAL OF SPORTS MEDICINE
(2021)
Review
Cell & Tissue Engineering
Zahra Azizi, Roya Abbaszadeh, Roxana Sahednasagh, Amir Norouzy, Elahe Motevaseli, Kathrin Maedler
Summary: Bone marrow mesenchymal stromal cells have anti-inflammatory and pro-survival properties, and their fusion with transplanted islets can improve diabetes treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Meng Zhu, Qiwei Wang, Tianning Gu, Yingli Han, Xin Zeng, Jinxin Li, Jian Dong, He Huang, Pengxu Qian
Summary: Haematopoietic Stem cells (HSCs) possess the ability to renew and differentiate into various lineages, which is regulated by the microenvironment. The use of hydrogels in tissue engineering has allowed for the mimicking of the HSC microenvironment in vitro, influencing HSC behavior and ultimately improving HSC acquisition. This review presents the recent advancements in hydrogel-based microenvironment engineering of HSCs and discusses future challenges in basic research and clinical practice.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Naoki Hosaka, Seiji Kanda, Takaki Shimono, Toshimasa Nishiyama
Summary: The study presents a new method for generating mouse gamma delta T cells by co-culturing HSC-enriched bone marrow cells with induced thymic epithelial cells derived from induced pluripotent stem cells. This method shows promise for clinical applications and could potentially be utilized for producing human gamma delta T cells as well.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Review
Endocrinology & Metabolism
Matthew J. Kim, Rodrigo J. Valderrabano, Joy Y. Wu
Summary: This article discusses various populations of osteoblasts at different stages of differentiation and summarizes the role of the osteoblast lineage in supporting hematopoiesis.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Immunology
Audrey Romano, Najmeeyah Brown, Helen Ashwin, Johannes S. P. Doehl, Jonathan Hamp, Mohamed Osman, Nidhi Dey, Gulab Fatima Rani, Tiago Rodrigues Ferreira, Paul M. Kaye
Summary: During Leishmania donovani infection, Ly6C(hi) monocytes are primed in the bone marrow in a process driven by CD4(+) T cells. IFN gamma promotes and IL-10 limits monocyte activation, while the presence of parasites/parasite antigen plays a crucial role in maintaining bone marrow monocyte activation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Yingying Sun, Xiaoqing Han, Chao Shang, Yawei Wang, Boya Xu, Shu Jiang, Yan Mo, Dake Wang, Yueshuang Ke, Xianlu Zeng
Summary: Tumors can modify myeloid cell differentiation and induce an immunosuppressive microenvironment. Granulocytic myeloid-derived suppressor cells (G-MDSCs), which are immature myeloid cells with immunosuppressive activity, exist in tumor-bearing hosts. This study reveals that the increase of granulocyte colony-stimulating factor (G-CSF) in mouse serum promotes G-MDSCs to obtain immunosuppressive traits in peripheral blood through the PI3K-Akt/mTOR pathway. Additionally, downregulation of type I interferon (IFN-I) signaling in G-MDSCs is a prerequisite for their immunosuppressive effects.
CELL DEATH & DISEASE
(2022)
Review
Cell & Tissue Engineering
Pan Zhang, Xiang Li, Chengwei Pan, Xinmin Zheng, Bohan Hu, Ruiheng Xie, Jialu Hu, Xuequn Shang, Hui Yang
Summary: Single-cell sequencing methods play a crucial role in studying cell heterogeneity and evolutionary relationships of stem cells, particularly in tracing the origin and lineage commitment of HSCs, profiling the bone marrow microenvironment, and dissecting malignant hematopoiesis with high resolution.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Immunology
Olga Trofimova, Ksenija Korotkaja, Dace Skrastina, Juris Jansons, Karina Spunde, Maria Isaguliants, Anna Zajakina
Summary: IFNg is a pleiotropic cytokine with potential antitumor immunomodulatory properties. The SFV/IFNg vector can induce a therapeutic antitumor T-cell response and inhibit myeloid cell infiltration in treated tumors, showing promising therapeutic effects.
Article
Biochemistry & Molecular Biology
Elia Bari, Ilaria Roato, Giuseppe Perale, Filippo Rossi, Tullio Genova, Federico Mussano, Riccardo Ferracini, Marzio Sorlini, Maria Luisa Torre, Sara Perteghella
Summary: A new strategy was developed to improve the osteoinductivity of SmartBone (SB) by loading the SB scaffolds with freeze-dried formulation of mesenchymal stem cell (MSC)-secretome. The study showed that SBlyo significantly enhanced cell proliferation and tissue formation compared to SB, suggesting a more effective bone formation induction by SBlyo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Constanza Savid-Frontera, Maria E. Viano, Natalia S. Baez, Della Reynolds, Mariana Matellon, Howard A. Young, Maria C. Rodriguez-Galan
Summary: This study demonstrates that utilizing IL-12 cDNA can safely treat tumors and control tumor growth in mouse models. Systemic IL-12 expression leads to a higher frequency of tumor-infiltrated cells and IFN-γ-producing CD8(+) T cells, along with a lower frequency of regulatory CD4(+)FOXP3(+) and CD11b(+)Gr-1(+) cells.
Article
Cell Biology
Marieke Goedhart, Edith Slot, Maria F. Pascutti, Sulima Geerman, Timo Rademakers, Benjamin Nota, Stephan Huveneers, Jaap D. van Buul, Katherine C. MacNamara, Carlijn Voermans, Martijn A. Nolte
Summary: Apart from controlling hematopoiesis, the bone marrow also functions as a secondary lymphoid organ. Bone marrow-resident dendritic cells (DCs) are mainly localized around sinusoids and can present antigens. They belong to the cDC2 subtype and are associated with Th2/Th17 immunity. These DCs express several c-type lectins, including Dectin-1, which can bind fungal beta-glucans. Compared to spleen DCs, bone marrow DCs are more efficient in phagocytosing yeast particles and Aspergillus conidia, and this is dependent on Dectin-1. Additionally, zymosan-stimulated bone marrow DCs enhance neutrophil differentiation and maintenance of progenitor cells.
Editorial Material
Cell Biology
Katherine C. MacNamara, Martijn A. Nolte
Biographical-Item
Cell Biology
Sharon M. Wahl, Howard A. Young
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Review
Immunology
Enitome E. Bafor, Julio C. Valencia, Howard A. Young
Summary: The immune regulation of female reproductive function plays a crucial role in fertility, disruptions in immune tolerance can lead to autoimmune subfertility or infertility. Double negative T regulatory cells (DNTregs) are emerging as effector cells capable of mediating immune tolerance in the female reproductive system, potentially providing a paradigm shift from the known roles of conventional regulatory T cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Weici Zhang, Tihong Shao, Patrick S. C. Leung, Koichi Tsuneyama, Luke Heuer, Howard A. Young, William M. Ridgway, M. Eric Gershwin
Summary: The combination therapy of anti-BAFF and anti-CD20 was found to be more effective in reducing antibody levels, depleting B cells, and improving clinical symptoms in patients with primary biliary cholangitis (PBC).
JOURNAL OF AUTOIMMUNITY
(2022)
Article
Multidisciplinary Sciences
Benoit P. Nicolet, Monika C. Wolkers
Summary: T cells play a crucial role in our defense against infections and malignancies. A study found that mRNA expression is a poor indicator of protein output in T cells, regardless of differentiation or activation status. However, a correlation was found between gene class-specific mRNA expression and protein expression, suggesting the role of gene characteristics in determining gene expression in T cells. The presence of AU-rich elements in the 3'UTR was also associated with mRNA and protein abundance in a T cell activation-dependent, gene class-specific manner.
Article
Immunology
Constanza Savid-Frontera, Maria Estefania Viano, Natalia S. Baez, Nicolas L. Lidon, Quentin Fontaine, Howard A. Young, Lene Vimeux, Emmanuel Donnadieu, Maria Cecilia Rodriguez-Galan
Summary: Virtual memory CD8(+) T cells (T-VM) have memory-like phenotype and respond to innate stimuli. Stimulation with IL-12 plus IL-18 leads to enrichment of T-VM cells in lymphoid organs and tumor models, and plays a role in tumor growth control.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Max L. B. Gronloh, Janine J. G. Arts, Sebastian Palacios Martinez, Amerens A. van Der Veen, Lanette Kempers, Abraham C. van Steen, Joris J. T. H. Roelofs, Martijn A. Nolte, Joachim Goedhart, Jaap D. van Buul
Summary: Using a photoconvertible probe, researchers traced and identified the original endothelial transmigration hotspots, and found that these hotspots function to limit vascular leakage during transmigration events. The distribution of ICAM-1 determines the location of the transmigration hotspot.
Article
Nutrition & Dietetics
Heekyong R. Bae, Su-Kyung Shin, Youngji Han, Ji-Hyeon Yoo, Suntae Kim, Howard A. Young, Eun-Young Kwon
Summary: The rare sugar D-allulose has potential benefits in addressing metabolic disorders such as obesity and type 2 diabetes (T2D) by improving insulin resistance through mitigating disruptions in macrophage and mitochondrial function.
Article
Biology
Eike K. Mahlandt, Sebastian Palacios Martinez, Janine J. G. Arts, Simon Tol, Jaap D. van Buul, Joachim Goedhart
Summary: This study investigates the role of Rho GTPases in the vascular barrier of endothelial cells using optogenetics. The results show that by precisely controlling the activation of Rho GTPases, the strength of the vascular barrier can be precisely regulated in a VE-cadherin-independent manner through cell-cell overlap-dependent mechanism. Furthermore, optogenetic control over morphological features of endothelial cells, such as cell size and shape, is also demonstrated.
Article
Medicine, General & Internal
Daan P. van den Brink, Derek J. B. Kleinveld, Annabel Bongers, Jael Vos, Joris T. H. Roelofs, Nina C. Weber, Jaap D. van Buul, Nicole P. Juffermans, Toshiaki Iba
Summary: In a rat pneumosepsis model, supplementing fluid resuscitation with heparan sulfate did not reduce organ or endothelial injury.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Max Laurens Bastiaan Gronloh, Janine Johanna Geertruida Arts, Eike Karin Mahlandt, Martijn A. Nolte, Joachim Goedhart, Jaap Diederik van Buul
Summary: During inflammation, primary neutrophil adhesion to the endothelium triggers the formation of transmigration hotspots, allowing secondary neutrophils to cross the endothelium more efficiently. This process is mediated by the activation of JNK1 and MARCKSL1, which induces the formation of filopodia and increases neutrophil adhesion.
Article
Immunology
Tihong Shao, Patrick S. C. Leung, Weici Zhang, Koichi Tsuneyama, William M. Ridgway, Howard A. Young, Zongwen Shuai, Aftab A. Ansari, M. Eric Gershwin
Summary: By inhibiting the JAK/STAT signaling pathway, the development and progression of autoimmune cholangitis can be effectively blocked. This study found that ruxolitinib treatment can reduce inflammation, lower autoantibody levels, and alter macrophage polarization, which holds important immunological and clinical significance for cholangitis.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
