Article
Environmental Sciences
Purushothaman Ramamoorthy, Sathishkumar Samiappan, Martin J. Wubben, John P. Brooks, Amrit Shrestha, Rajendra Mohan Panda, K. Raja Reddy, Raju Bheemanahalli
Summary: The study examines the impact of root-knot nematode (RKN) and drought stress (DS) on cotton plants. Results show that RKN stress can be detected as early as 10 days after infestation, and RKN, DS, and DS + RKN can be detected with an accuracy of over 98% using specific wavelength bands. Different cotton genotypes showed varied responses to the stresses, with hyperspectral sensor data being able to distinguish RKN-infected plants from drought-stressed plants.
Article
Agronomy
Top Bahadur Pun, Arjun Neupane, Richard Koech
Summary: This study developed a semi-automated method to quantify root-knot nematodes (RKN) based on size using image analysis, with three novel approaches showing good performance in detecting and quantifying RKN length, with the SG method being the most suitable for this purpose.
Article
Multidisciplinary Sciences
Nathalia Fitoussi, Eli Borrego, Michael V. Kolomiets, Xue Qing, Patricia Bucki, Noa Sela, Eduard Belausov, Sigal Braun Miyara
Summary: This study investigated the response of oxylipins in tomato roots upon infection by root-knot nematodes and identified genes that respond to the lipidomic defense pathway mediated through oxylipins using RNA-Seq. Analysis of differentially expressed genes revealed some potential effectors, including those involved in distorting plant immune responses or manipulating signal transduction mediated by lipid signals.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
M. S. Soliman, M. M. El-Deriny, D. S. S. Ibrahim, H. Zakaria, Y. Ahmed
Summary: The study demonstrates that Arthrobotrys oligospora has significant suppressive and predacious effects against root-knot nematode, M. incognita, while also promoting plant growth.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Raghda Zuhair, Yasser Thabet A. Moustafa, Nabil S. A. Mustafa, Mohamad F. El-Dahshouri, Lixin Zhang, Mohamed F. Ageba
Summary: The study showed that vermicomposts from different plant residues sources can significantly promote the growth of tomato plants infected with root-knot nematodes while effectively controlling the nematode infection. This type of vermicompost serves not only as a bio-organic fertilizer but also as an effective bio-controlling treatment for root-knot nematodes, with no negative impact on the environment.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Agricultural Engineering
Alessandro Benelli, Chiara Cevoli, Angelo Fabbri, Luigi Ragni
Summary: In this study, hyperspectral imaging was used to evaluate the ripeness of 'Hayward' kiwifruit and develop models for predicting soluble solids content and flesh firmness. The results showed that hyperspectral imaging is suitable for predicting kiwi quality attributes and their classification.
BIOSYSTEMS ENGINEERING
(2022)
Article
Plant Sciences
Fulin Zhang, Juan Wang, Xianguo Li, Jun Zhang, Yuxiang Liu, Yijia Chen, Qinghui Yu, Ning Li
Summary: Phenylalanine ammonia-lyase (PAL) is a key and rate-limiting enzyme in the phenylpropanoid metabolism pathway in plants. This study identified and characterized PAL family genes in tomato and studied their expression after root-knot nematode infection. The findings provide insights into the functions of PAL genes in tomato and their potential application in resistance against root-knot nematodes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Yuji Oka
Summary: In this study, it was found that the combination of fluensulfone with certain aromatic compounds can attract Meloidogyne juveniles, while fluopyram alone is attractive to Meloidogyne juveniles, particularly M. javanica.
PEST MANAGEMENT SCIENCE
(2023)
Review
Horticulture
Shilpa, Parveen Sharma, Vandana Thakur, Akhilesh Sharma, R. S. Rana, Prabhat Kumar
Summary: The importance of tomato as a vegetable and salad product is evident, but various biological stressors, particularly root-knot nematodes, significantly hinder tomato productivity. Current management methods are insufficient to control root-knot nematodes completely. Therefore, integrated approaches including genetics-based techniques have become important for the control of root-knot nematodes in tomatoes.
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Priyanka Reddy, Joe Panozzo, Kathryn M. Guthridge, German C. Spangenberg, Simone J. Rochfort
Summary: Using NIR-HSI, researchers were able to distinguish the presence of NEA12 fungal endophyte and different cultivars of perennial ryegrass seed. This study demonstrates the potential of NIR-HSI reflectance data for discrimination and identification purposes.
Article
Agronomy
Chunshan Wang, Shedong Sun, Chunjiang Zhao, Zhenchuan Mao, Huarui Wu, Guifa Teng
Summary: This study proposes a cucumber root-knot nematode detection model based on the modified YOLOv5s model (YOLOv5-CMS), which enhances the model's ability to extract key features, optimizes the cluster algorithm, and improves the detection precision. Experimental results show that the YOLOv5s-CMS model achieves better performance compared to the original model, providing more intuitive and accurate data sources for breeding cucumber varieties resistant to root-knot nematode.
Article
Multidisciplinary Sciences
Theodore W. Kahn, Nicholas B. Duck, Michael T. McCarville, Laura Cooper Schouten, Kathryn Schweri, Jelena Zaitseva, Julia Daum
Summary: The study reveals that soybean lines expressing the Bacillus thuringiensis protein Cy14Ab offer significant protection against soybean cyst nematode (SCN) in both greenhouse and field trials, showing promising potential for controlling plant-parasitic nematodes (PPNs). The mechanism of action involves damaging the nematodes' intestines, similar to how Cry proteins control insects, resulting in a reduction in cyst numbers and egg counts in transgenic soybean plants expressing Cry14Ab.
NATURE COMMUNICATIONS
(2021)
Article
Agriculture, Multidisciplinary
Hui Feng, Can-rong Zhou, Feng Zhu, Xiu-hu Le, De-dao Jing, Paul Daly, Dong-mei Zhou, Li-hui Wei
Summary: This study provides a detailed characterization of a novel source of resistance to Meloidogyne graminicola in rice. The rice variety Huidao 5 attracts the root nematodes but hinders their movement, resulting in reduced nematode numbers. The resistance is also observed under upland and flooding conditions. Cross breeding experiments suggest that a dominant gene controls the resistance. This study highlights the potential of this resistance source for crop breeding.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Agronomy
Asmaa Khader, Mahmoud Ibrahim, Fahad Alkhathami, Hany Elsawy, Nasser Abd El-Kader, Eman Shaker, Azza Sedky, Esawy Mahmoud
Summary: The use of green nano-amendments, such as green nanobiochar, showed promising results in improving soil health and reducing root-knot nematodes, thereby increasing tomato yield. These nano-amendments increased soil organic matter and microbial biomass while significantly decreasing the population of nematodes, leading to improved tomato growth.
Article
Plant Sciences
Shixiao Xu, Pei Tian, Zhimin Jiang, Xiaoxiang Chen, Bo Li, Jutao Sun, Zhiqiang Zhang
Summary: In this study, the transcriptional dynamics and metabolic processes involved in the interaction between RKN-resistant and RKN-susceptible tobacco varieties infected with PVY MSNR were investigated using RNA-seq analysis and functional enrichment analysis. The results revealed significant differences in gene expression and metabolic pathways, providing insights into the resistance mechanisms of tobacco against PVY MSNR.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Zoology
Anastasija Panevska, Matej Skocaj, Spela Modic, Jaka Razinger, Kristina Sepcic
Summary: Aegerolysin proteins produced by Pleurotus mushrooms interact specifically with sphingolipid-enriched membranes, showing strong toxicity towards insect cells. Their mode of membrane binding differs from bacterial origin complexes, and can potentially be utilized in treatments and insect control.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Evelin Despot-Slade, Brankica Mravinac, Sasa Sirca, Philippe Castagnone-Sereno, Miroslav Plohl, Nevenka Mestrovic
Summary: Research on evolution of CenH3 in Meloidogyne species suggests subspecialization of CenH3s in ancestral sexual species. In mitotic Meloidogyne incognita, alpha CenH3 plays a dominant role at the centromere, while other CenH3s lose function in mitosis. The absence of centromere drive in mitosis may allow for an equilibrium between CenH3 and associated DNA constituents to persist over time.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Chemistry, Multidisciplinary
Craig Mayall, Andraz Dolar, Anita Jemec Kokalj, Sara Novak, Jaka Razinger, Francesco Barbero, Victor Puntes, Damjana Drobne
Summary: The study compared the immune responses of Porcellio scaber to different stressors, showing that both feeding and injection of nanoparticles (NPs) caused immune responses, but the type of stressor significantly impacted the response.
Article
Environmental Sciences
Uros Zibrat, Barbara Geric Stare, Matej Knapic, Nik Susic, Janez Lapajne, Sasa Sirca
Summary: The study showed that Meloidogyne luci may develop a latent infestation without visible symptoms on potato tubers, posing a high risk for uncontrolled spread of the pest. Efficient detection methods using hyperspectral imaging and real-time PCR were developed to prevent this spread. Detection with hyperspectral imaging achieved a 100% success rate while real-time PCR showed high sensitivity in detecting M. luci.
Article
Food Science & Technology
Matej Milijas Jotic, Anastasija Panevska, Ioan Iacovache, Rok Kostanjsek, Martina Mravinec, Matej Skocaj, Benoit Zuber, Ana Pavsic, Jaka Razinger, Spela Modic, Francesco Trenti, Graziano Guella, Kristina Sepcic
Summary: OlyA6 is a protein produced by oyster mushrooms that binds strongly to insect-specific membrane sphingolipid CPE. Together with PlyB, it exhibits potent insecticidal activity against the western corn rootworm by forming transmembrane pore complexes in the insect midgut. The molecular mechanism of action involves specific interactions with CPE, leading to pore formation in the insect midgut.
Article
Biology
Maela Leon, Monica Berbegal, Paloma Abad-Campos, Antonio Ramon-Albalat, Tito Caffi, Vittorio Rossi, Gultakin Hasanaliyeva, Pierre Antoine Noceto, Daniel Wipf, Sasa Sirca, Jaka Razinger, Anne-Laure Fragniere, Patrik Kehrli, Aurora Ranca, Anamaria Petrescu, Josep Armengol
Summary: The study found that black-foot disease-associated fungi can be present on the roots of cover crops grown in organic vineyards, potentially increasing inoculum levels in vineyard soils. Results showed that various fungal species related to black-foot disease were found on cover crops in several European countries.
Article
Entomology
Maarten de Groot, Magda Rak Cizej, Andreja Kavcic, Spela Modic, Francek Policnik, Nina Sramel, Primoz Zigon, Jaka Razinger
Summary: Forests influence the populations of spotted wing Drosophila, with fewer individuals found farther away. There is a difference in abundance between males and females, with males being less abundant farther from the forest. Potential control measures for the pest in raspberry orchards can be proposed based on these findings.
JOURNAL OF APPLIED ENTOMOLOGY
(2022)
Article
Chemistry, Analytical
Janez Lapajne, Matej Knapic, Uros Zibrat
Summary: Hyperspectral imaging is a popular tool for non-invasive plant disease detection, but the acquired data often contain redundancies. In this study, six dimensionality reduction methods were tested on a hyperspectral dataset of potato tubers. The results showed that all methods successfully identified the infected tubers, with linear discriminant analysis performing the best.
Article
Biology
Evelin Despot-Slade, Sasa Sirca, Brankica Mravinac, Philippe Castagnone-Sereno, Miroslav Plohl, Nevenka Mestrovic
Summary: Our study conducted a comprehensive analysis of satellitomes in closely related Meloidogyne spp. nematodes. The evolutionary trends of satDNAs suggest that polyploidization has led to the addition of new sets of satDNAs in the satellitome of each Meloidogyne species. We also identified conserved sequence motifs and regions in certain satDNAs, indicating sequence evolution under selective pressure. Furthermore, we found actively transcribed satDNAs in related genomes and throughout nematode development, suggesting that their expression is not random.
Article
Entomology
Lara Reinbacher, Eva Praprotnik, Jaka Razinger, Sven Bacher, Giselher Grabenweger
Summary: The study found that plant diet affects the growth and development of wireworm larvae, but has little impact on their susceptibility to fungal infection. The choice of cover crop also affects the damage caused by wireworms on cash crops.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Agronomy
Eva Praprotnik, Andrej Voncina, Primoz Zigon, Matej Knapic, Nik Susic, Sasa Sirca, Dominik Vodnik, David Lenarcic, Janez Lapajne, Uros Zibrat, Jaka Razinger
Summary: The research aimed to evaluate the use of hyperspectral imaging for early detection and discrimination of biotic and abiotic stress in maize. The effects of wireworm feeding and drought stress on maize plants were investigated, focusing on leaf spectral responses and various morphological and physiological traits. Hyperspectral imaging was able to detect pest infestation and drought stress earlier than classical methods, with the highest accuracy observed on day 14.
Article
Multidisciplinary Sciences
Anastasija Panevska, Nastja Cegovnik, Klavdija Fortuna, Alen Vukovic, Maja Grundner, Spela Modic, Gregor Bajc, Matej Skocaj, Martina Mravinec Bohte, Lara Larisa Poposek, Primoz Zigon, Jaka Razinger, Peter Veranic, Natasa Resnik, Kristina Sepcic
Summary: OlyA6 protein binds to cholesterol-complexed sphingomyelin and can label lipid rafts. It forms pores in insect midgut cell membranes and acts as a potent bioinsecticide when combined with pleurotolysin B. A point mutation (E69A) enhances the interaction of OlyA6 with sphingomyelin and CPE, allowing recognition of these sphingolipids even in the absence of cholesterol. However, the mutation does not improve its membrane-permeabilizing activity after addition of pleurotolysin B.
SCIENTIFIC REPORTS
(2023)
Article
Horticulture
Nika Cvelbar Weber, Jaka Razinger, Jerneja Jakopic, Valentina Schmitzer, Metka Hudina, Ana Slatnar, Robert Veberic, Franci Stampar, Tilen Zamljen
Summary: The polyphagous brown marmorated stink bug is an important pest that can feed on and cause damage to strawberries. Its attacks lead to a decrease in sugar content and an increase in phenolic compounds in the strawberries, impacting the plant's secondary metabolism. Infested fruit are not suitable for commercial production due to off-flavors produced.
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)