Review
Biochemistry & Molecular Biology
Magdalena Hryhorowicz, Daniel Lipinski, Joanna Zeyland
Summary: The bacteria-derived CRISPR/Cas system is a versatile tool for genome engineering, with wide applications in various fields. However, challenges like immunological reactions and off-target effects remain. This review discusses the current classification of CRISPR systems, new genome-editing technologies, recent applications, as well as limitations, ethical issues, and challenges associated with the CRISPR/Cas system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pathology
V. Edwin Hillary, Savarimuthu Ignacimuthu, S. Antony Ceasar
Summary: Multiple research groups are exploring the potential of CRISPR-based tools for rapid detection of COVID-19, with expectations for novel kits for mass detection in the future, indicating optimism that science will ultimately triumph over the pandemic.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Review
Chemistry, Analytical
Muhammad Sohail, Siying Xie, Xing Zhang, Bingzhi Li
Summary: CRISPR/Cas-based analytical procedures have revolutionized the sensing platform and have various applications in the detection of nucleic acid and non-nucleic acid analytes. This review highlights different CRISPR/Cas reporters, signal transducers, and sensing strategies, as well as the challenges and requirements for their practical use.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
A. A. Vasileva, S. A. Aliukas, P. A. Selkova, A. N. Arseniev, V. E. Chernova, O. S. Musharova, E. I. Klimuk, M. A. Khodorkovskii, K. V. Severinov
Summary: The use of CRISPR-Cas bacterial adaptive immunity system components has opened up new possibilities for programmable genome editing in higher organisms. The most commonly used gene editors are based on the Cas9 effectors of type II CRISPR-Cas systems. This paper presents a workflow for the search and characterization of new Cas9 nucleases, including bioinformatical search, cloning, isolation, in vitro nuclease activity testing, and determination of the required PAM sequence.
Review
Biochemistry & Molecular Biology
Pooja Bhardwaj, Rajni Kant, Sthita Pragnya Behera, Gaurav Raj Dwivedi, Rajeev Singh
Summary: This article emphasizes the importance of CRISPR/Cas technology as a diagnostic tool for early management, diagnosis, and treatment of infections and diseases, and explores its detection capabilities for non-nucleic acid targets as well as the technical challenges and improvements in clinical assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Jie Xu, Zhujun Liu, Zhen Zhang, Tongbo Wu
Summary: The trans-cleavage activity of Cas12a is affected by the length of the fluorescent probe and the reaction buffer. The optimal conditions for Cas12a are a probe length of 15 nucleotides and NEBuffer 4 as the buffer, resulting in a 50-fold improvement in activity and a significant decrease in the detection limit for DNA targets. This method provides a powerful tool for Cas12a trans-cleavage activity applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Daniel M. Sapozhnikov, Moshe Szyf
Summary: PROTECTOR is a novel approach that uses a nuclease-dead Cas protein to bind to off-target sites and interfere with Cas activity, reducing off-target mutation rates without compromising on-target activity. It can be used in combination with high-fidelity Cas enzymes to further decrease off-target editing, offering an ability to protect off-target sites with identical sequences to target sites.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Florian Stoertz, Peter Minary
Summary: With the advancement of CRISPR/Cas programmable nuclease system, in vivo therapeutic gene editing applications are becoming more feasible. However, concerns over non-negligible off-target effects remain prominent. crisprSQL is an interactive collection of CRISPR/Cas9 off-target cleavage studies aimed at enriching cleavage profiling, gene editing safety analysis, and transcriptomics.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Sara M. Klose, Nadeeka Wawegama, Fiona M. Sansom, Marc S. Marenda, Glenn F. Browning
Summary: In this study, the researchers used the Mycoplasma gallisepticum CRISPR/Cas system to targeted mutate a nuclease gene, providing insights into the role of this gene and revealing the effectiveness of the system in interfering with gene functions.
VETERINARY MICROBIOLOGY
(2022)
Review
Cell Biology
Yanping Hu, Wei Li
Summary: Abundant CRISPR-Cas systems provide valuable resources for developing versatile tools in biological discovery and disease treatment. This review discusses the development of CRISPR-Cas based tools and explores potential directions for future novel CRISPR toolkits.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Plant Sciences
Andrea Giordano
Summary: CRISPR/Cas technology is a versatile genome editing system that is being used to address various challenges in agriculture. Tang et al. (2023) combined CRISPR/Cas9 with Agrobacterium virulence proteins and achieved a significant increase in genome editing frequency. This system was validated in rice and has the potential to be applied in genome engineering of agronomic traits and other species in the future.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Chemistry, Analytical
Jiri Kudr, Petr Michalek, Lada Ilieva, Vojtech Adam, Ondrej Zitka
Summary: The COVID-19 pandemic has created a demand for improvement and development of diagnostic methods, with electrochemical biosensors showing promise as rapid, low-cost, and user-friendly tools. This critical review outlines the molecular properties of SARS-CoV-2 and discusses the advantages and drawbacks of conventional diagnostic methods while highlighting the potential of electrochemical biosensors. Future perspectives on viral electrochemical biosensor development are briefly mentioned.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Helen B. Belato, George P. Lisi
Summary: The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs), has revolutionized the field of CRISPR-Cas technology. These inhibitors have diverse structures and functions, and can act as orthosteric or allosteric inhibitors, as well as modify CRISPR-Cas components. Understanding the mechanisms of Acrs is crucial for the development of more precise and controllable CRISPR-Cas tools.
Review
Oncology
Alien Balian, Frank J. Hernandez
Summary: Early cancer diagnosis is crucial for improved treatment options and survival rates, and the search for accurate cancer diagnostic biomarkers remains an ongoing challenge. Nucleases, enzymes capable of cleaving nucleic acids, have been considered as potential cancer biomarkers due to their biological functions and enzymatic activity. This review discusses the potential of harnessing the enzymatic activity of cancer-associated nucleases as a novel diagnostic biomarker using nucleic acid probes as substrates.
BIOMARKER RESEARCH
(2021)
Article
Biochemical Research Methods
Xiang Sun, Dan Ohtan Wang, Jinkai Wang
Summary: This review summarizes the latest CRISPR-Cas13 and Cas9 toolkits for site-specific manipulation of m(6)A, including fundamental components, on-target efficiency, editing window, PAM/PFS requirement, subcellularly localized targeting, and potential limitations. It aims to provide an overview to assist researchers in choosing optimal tools for manipulating m(6)A for different purposes and also points out possible optimization strategies.
Article
Infectious Diseases
Joung Ha Park, Bonhan Koo, Min Jae Kim, Hyo Joo Lee, Hye-Hee Cha, Ji Yeun Kim, Sei Won Lee, Yong Shin, Sung-Han Kim
Summary: Plasma cfDNA detection shows promising sensitivity and specificity for diagnosing tuberculosis, especially when used in combination with interferon-gamma releasing assay (IGRA) for ruling out TB.
INFECTIOUS DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Jiyeon Kweon, Hye-Yeon Hwang, Haesun Ryu, An-Hee Jang, Daesik Kim, Yongsub Kim
Summary: Researchers developed a method using prime editor 2 nuclease (PE2 nuclease) and paired pegRNA to achieve programmable chromosomal translocations and inversions. They successfully introduced DNA recombination and precise chromosomal translocations in human cells. This method can be used to create cancer-associated translocations and inversions as well as for disease modeling or gene therapy.
Article
Hematology
Hyunji Kim, Daehyun Chu, Miyoung Kim, Young-uk Cho, Chan-jeoung Park, Seongman Bae, Min Jae Kim, Yong Pil Chong, Seongsoo Jang, Sung-Han Kim
Summary: This study suggests that Lupus anticoagulants (LA) are associated with disease severity in COVID-19 patients. The majority of LA-positive patients showed LA positivity within 10 days of SARS-CoV-2 infection, while a subgroup of patients initially tested negative for LA during the early stages of infection and became positive later.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Correction
Medicine, General & Internal
Jiwon Jung, Sun-Kyung Kim, Yumi Lee, Soyeon Park, Young-Ju Lim, Eun Ok Kim, Sung-Han Kim
JOURNAL OF KOREAN MEDICAL SCIENCE
(2023)
Article
Medicine, General & Internal
Sungim Choi, Song Mi Moon, Ji-Soo Kwon, Sung-Han Kim, Seong Yeon Park
Summary: The study evaluated the diagnostic usefulness of varicella zoster virus (VZV) specific IgA and IgG antibodies in patients with herpes zoster (HZ). The results showed significant differences in the levels of VZV-specific IgA and IgG antibodies between HZ patients and healthy controls.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2023)
Article
Medicine, General & Internal
Jiyoung Lee, Euijin Chang, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang -Oh Lee, Sang -Ho Choi, Yang Soo Kim, Seongman Bae
Summary: This study evaluated the rate and composition of bacterial co-infection in COVID-19 patients and assessed the association between the use of empirical antibacterials and mortality. The results showed that bacterial co-infection was infrequent in the patients, and the co-infected organisms varied depending on the previous use of antibacterial drugs.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2023)
Article
Dermatology
Hyejin Park, Seongman Bae, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Ho Choi, Sang-Oh Lee, Yang Soo Kim, Jiwon Jung
Summary: This study retrospectively analyzed the clinical characteristics and outcomes of invasive and non-invasive fusariosis in South Korea. Invasive fusariosis mainly affected immunocompromised patients and was associated with a poor prognosis. Non-invasive fusariosis was common in patients with diabetes and solid cancer.
Article
Oncology
Jae Sik Kim, Joo Ho Lee, Sang-Rok Jeon, Yongsub Kim, Seung Hyuck Jeon, Hong-Gyun Wu
Summary: This study used a CRISPR-based knockout screen to determine the mechanism of EGF-induced apoptosis and identified 266 genes that may be responsible for EGF-induced apoptosis. The experimental validation showed that DUSP1 may be a critical component of EGF-induced apoptosis and a potential target for EGFR-overexpressing cancers.
CANCER RESEARCH AND TREATMENT
(2023)
Letter
Infectious Diseases
Song Hee Namgung, Jiwon Jung, Sun Kyung Kim, Eun Ok Kim, Kyung-Wook Jo, Tae Sun Shim, Sung-Han Kim
JOURNAL OF INFECTION
(2023)
Article
Biochemistry & Molecular Biology
Eun-Young Kim, Ji-Eun Kim, Young-Eun Kim, Bongkun Choi, Dong Hyun Sohn, Si-On Park, Yeon-Ho Chung, Yongsub Kim, William H. Robinson, Yong-Gil Kim, Eun-Ju Chang
Summary: The study found that parkin dysfunction associated with the progression of parkinsonism contributes to low bone mineral density. The decreased parkin in monocytes is linked to increased bone-resorbing activity of osteoclasts. Parkin-deficient mice exhibit an osteoporotic phenotype with increased bone-resorbing capacity and susceptibility to inflammatory arthritis.
CELL AND BIOSCIENCE
(2023)
Article
Medicine, Research & Experimental
Gayoung Jang, Ha Rim Shin, Hyo-Sang Do, Jiyeon Kweon, Soojin Hwang, Soyoung Kim, Sun Hee Heo, Yongsub Kim, Beom Hee Lee
Summary: This study utilized CRISPR-mediated base editors (BEs) and prime editors (PEs) to generate LNS-associated disease models and correct the disease models, suggesting a potential therapeutic strategy for this rare genetic disease.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Chemistry, Analytical
Hyo Joo Lee, Nam Hun Kim, Eun Hye Lee, Young Soon Yoon, Yun Jeong Jeong, Byung Chul Lee, Bonhan Koo, Yoon Ok Jang, Sung-Han Kim, Young Ae Kang, Sei Won Lee, Yong Shin
Summary: This study proposes a simple tuberculosis molecular diagnostic assay that combines sample preparation and DNA detection steps. The sample preparation is done using a syringe filter with amine-functionalized diatomaceous earth and homobifunctional imidoester, and the target DNA is detected using quantitative PCR. The system has a detection limit that is 10 times higher than conventional PCR assays and can provide results within 2 hours from large volume samples without additional instruments. The clinical utility of the proposed method was validated in 88 sputum samples from four hospitals in the Republic of Korea, showing superior sensitivity compared to other assays. Therefore, this system can be useful for MTB diagnosis in limited-resource settings.
Article
Immunology
Jin Young Ahn, Jae-Hoon Ko, Kyong Ran Peck, Seongman Bae, Sung-Han Kim, Kyoung Hwa Lee, Young Goo Song, Yong Chan Kim, Yoon Soo Park, Kyoung-Ho Song, Eu Suk Kim, Hye Won Jeong, Shin-Woo Kim, Ki Tae Kwon, Won Suk Choi, Jun Yong Choi
Summary: This study investigated the long-term kinetics of immune responses after heterologous booster vaccination and the impact of Omicron breakthrough infection. The findings showed that participants who experienced breakthrough infection during the BA.1/BA.2 period had a more robust increase in neutralizing antibody titers against the wild-type virus compared to BA.1 and BA.5.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Gahee Kim, Ree Nar Yoo, Hyejin So, Jeong-Young Lee, Mi-Na Kim, Sung-Han Kim, Won Kyoung Jhang, Seong Jong Park, Jina Lee
Summary: Ralstonia mannitolilytica is a causative organism of nosocomial infections, particularly associated with contaminated water, and resistant to various antibiotics, including carbapenems. In this study, 18 patients with R. mannitolilytica infection were included, with 94.4% diagnosed with pneumonia and 5.6% with central line-associated bloodstream infection. The all-cause 30-day mortality rate was 61.1%, and environmental sampling identified R. mannitolylica at a cold-water tap of a water purifier and an exhalation circuit of a patient mechanical ventilator.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jiyeon Kweon, An-Hee Jang, Eunji Kwon, Ungi Kim, Ha Rim Shin, Jieun See, Gayoung Jang, Chaeyeon Lee, Taeyoung Koo, Seokjoong Kim, Yongsub Kim
Summary: This article introduces the use of various CRISPR-Cas9 orthologs in genome engineering. One of the smallest Cas9 orthologs, cjCas9, derived from Campylobacter jejuni, has been developed into base editors capable of inducing endogenous base substitutions with high efficiency and specificity. The engineered cjCas9 and gRNA scaffolds can further improve the base editing efficiency of cjABE8e. The findings in this study expand the potential of using base editors for therapeutic approaches.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
