期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 257, 期 -, 页码 496-503出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.10.177
关键词
Paper based screen-printed electrode; CEA; Quinone; Electrochemical sensor; Thiol-amine functionalization
In this study, a low cost and robust paper based electrochemical immunosensor was developed using self-made gold nanoparticle electrode for rapid and selective detection of Carcinoembryonic antigen (CEA). The working and counter electrode were printed using gold nanoparticle ink while the reference electrode was made using commerical silver ink. A simple mercapto-amine functionalised receptor (R1) based on moiety Quinone compound was formed on the paper based screen-printed gold (Au) electrode (P-SPGE) for highly selective sensing of CEA. A self-assembled monolayer principle (SAM) was exploited to fabricate the sensitive and selective immunosensor electrodes. In electrode fabrication, SAM layer was accomplished using covalent linkage of thiol in the R1 compound on the P-SPGE surface. Immobilised amine served as a layer for further binding of biological components. CEA levels were examined quantitatively using differential pulse voltammetry (DPV) and limit of detection (LoD) was calculated as 0.33 ng mL(-1). The performance of P-SPGE was compared with the commercial screen-printed Au electrode. A novel P-SPGE functionalised with R1 exhibited the tremendous performance for CEA detection in a linear concentration range of 1.0 ng mL(-1)-100.0ng mL(-1). The performance of this electrochemical immunosensor were carried out using different proteins. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据