Article
Multidisciplinary Sciences
Fan Jiang, Lei Wang, Nana Jin, Jing Yuan, Yanbin Li, Jianhan Lin
Summary: An electrochemical biosensor combining magnetic grid based bacterial separation and enzymatic catalysis based signal amplification was developed for Salmonella detection. The sensor was able to detect 50 CFU/mL Salmonella typhimurium in 1 hour.
Article
Chemistry, Analytical
Ana Diaz-Fernandez, Elena Bernalte, Carlos Fernandez-Ramos, Sandhya Moise, Pedro Estrela, Mirella Di Lorenzo
Summary: Y chimeric antigen receptor T (CAR-T) cell therapy has revolutionized cancer treatment. Online biosensors can monitor the effectiveness of engineered CAR-T cells in real time without the need for offline analysis or specialized personnel. This study presents an innovative label-free immunosensor that can detect CD34(+) T-cells in human serum, demonstrating high selectivity and applicability for clinical use.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Chang-Ho Han, Jaesung Jang
Summary: An immunosensor utilizing electrohydrodynamic focusing and DEP concentration was developed for continuous and label-free detection of flowing Staphylococcus aureus. The sensor demonstrated good linearity in detecting S. aureus concentrations, with a capture time of 35 min and a limit of detection of 150 CFU mL(-1). The use of electrical manipulation and biological interaction also provided high specificity in measurements.
Article
Chemistry, Analytical
Qingcao Li, Zhen Guo, Xuedan Qiu, Wenjun Lu, Wei Yang, Qilai Wang, Qiaoping Wu
Summary: This study introduced a LM-imprinted polymer-based sensor for the detection of Listeria monocytogenes, demonstrating high sensitivity and a wide linear range for LM detection. The sensor showed good recognition and capture ability for LM cells, with optimized conditions for achieving highly sensitive detection. Inter-electrode reproducibility, stability, selectivity, and applicability of the sensor were also investigated and showed acceptable results.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Yuki Saito, Shu Kobayashi
Summary: The application of heterogeneous catalysts in continuous-flow enantioselective hydroacylation reactions was studied. Catalysts were prepared by mixing supports and rhodium complexes, and showed excellent activity and enantioselectivity. Under optimized conditions, the catalysts exhibited high turnover numbers and could be applied to various substrates. Furthermore, sequential-flow reactions with other heterogeneous catalysts were demonstrated for the synthesis of biologically active molecules and functional materials.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Analytical
Yu-Shih Chen, Chun-Hao Huang, Ping-Ching Pai, Jungmok Seo, Kin Fong Lei
Summary: Electrical impedance biosensors, embedded with microfluidic technologies, have the potential to greatly improve sensitivity, reduce reagent consumption, and shorten analysis time. Microfluidics-enabled automation enhances the reliability and efficiency of analyses. The integration of electrical impedance biosensors with microfluidic systems enables the development of portable and user-friendly devices.
Article
Biophysics
Jie Chen, Kai -Chun Lin, Shalini Prasad, David W. Schmidtke
Summary: In this paper, a novel label-free biosensor for real-time monitoring of acetylcholine was demonstrated. The sensor showed a linear relationship between acetylcholine concentration and Delta Zmod, and it remained responsive in complex environments and even after implantation in rat brain tissue.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Li Xue, Ruya Guo, Fengchun Huang, Wuzhen Qi, Yuanjie Liu, Gaozhe Cai, Jianhan Lin
Summary: A biosensor using magnetic nanobead nets and manganese dioxide nanoflowers was developed for rapid and sensitive detection of Salmonella typhimurium, showing high accuracy in pathogenic bacteria screening in food.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Fengchun Huang, Li Xue, Wuzhen Qi, Gaozhe Cai, Yuanjie Liu, Jianhan Lin
Summary: An impedance biosensor using rotary magnetic separation and cascade reaction was developed for rapid and ultrasensitive detection of Salmonella typhimurium. The sensor utilizes magnetic nanoparticles modified with antibodies to capture target bacteria and employs hybrid nanoflowers to label the bacteria, resulting in impedance changes that can be measured to determine bacterial concentration. This biosensor showed excellent sensitivity and detection capabilities in a short period of time.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemical Research Methods
Pablo C. Serrano, Gisele E. Nunes, Lindiomar B. Avila Jr, Carleane P. S. Reis, Aldo M. C. Gomes, Francoise T. Reis, Maria L. Sartorelli, Silvia Pedroso Melegari, William G. Matias, Ivan H. Bechtold
Summary: Saxitoxin, a harmful cyanotoxin, requires monitoring in water bodies. An electrochemical impedimetric biosensor using a specific aptamer was developed for its detection, showing high sensitivity and selectivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Tajiguli Yimamumaimaiti, Qiwen Su, Rong-bin Song, Yan Gao, Sha Yu, Tingting Tan, Linlin Wang, Jun-Jie Zhu, Jian-Rong Zhang
Summary: Researchers have developed a continuous separation and detection platform for exosomes (EXOs) and host cancer cells in human serum. The platform integrates an enzymatic biofuel cell (EBFC)-powered biosensor and a flow cell-supported membrane separation device (FMSC), enabling fast, accurate, and nondestructive separation and detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Cheol-Kyun Choi, Samy M. Shaban, Byeong-Seok Moon, Do-Gi Pyun, Dong-Hwan Kim
Summary: This study developed a rapid and reliable colorimetric urea biosensor assisted by a smartphone, with high selectivity, sensitivity, and reliability. It enables direct diagnosis of human urine and paves the way for daily monitoring systems of renal and hepatic dysfunction diseases.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Sura A. Muhsin, Muthana Al-Amidie, Zhenyu Shen, Zahar Mlaji, Jiayu Liu, Amjed Abdullah, Majed El-Dweik, Shuping Zhang, Mahmoud Almasri
Summary: A microfluidic based biosensor was used for rapid and simultaneous detection of Salmonella, Legionella, and Escherichia coli O157:H7 in tap water and wastewater. The biosensor concentrated and detected bacteria at both low and high concentrations. The results showed that the biosensors could detect the three bacterial pathogens simultaneously with a detection limit of 3 bacterial cells/mL in 30-40 minutes.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Insu Kim, Yonghwan Kim, Sang Won Lee, Dongtak Lee, Hyo Gi Jung, Jae Won Jang, Taeha Lee, Young Kyung Yoon, Gyudo Lee, Dae Sung Yoon
Summary: In this study, erythrocyte-camouflaged biosensors (ECB) were fabricated for sensitive detection of Staphylococcus aureus-secreted alpha-hemolysin (Hla) using erythrocyte membrane (EM) extracted from human whole blood as a novel bioreceptor. The ECB showed higher sensitivity to Hla compared to major blood proteins, with signal intensities 8.8-12.7 times higher than those of the same concentration of blood proteins. Additionally, the ECB exhibited a detection limit of 1.9 ng/ml for Hla with a dynamic range of 0.0001-1 mg/ml and maintained constant sensing performance with 99.0 +/- 5.6% accuracy during 35 days of storage.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Applied
Yijian Wang, Liqin Kong, Guoqiang Shu, Guanqquan Sun, Yaoze Feng, Ming Zhu
Summary: An electrochemical impedimetric biosensor was developed for detecting umami substances. The biosensor showed low detection limits and wide linear ranges for monosodium glutamate and inosine-5'-monophosphate. It also exhibited high specificity in real food samples and desirable storability.
Article
Nanoscience & Nanotechnology
Xinge Xi, Isabelle I. Niyonshuti, Ningxiang Yu, Lan Yao, Ying Fu, Jingyi Chen, Yanbin Li
Summary: A label-free biosensor was developed in this study that required only one incubation step and achieved highly sensitive and specific detection of thrombin through target-triggered release of cargo molecules from gold nanocages. The biosensor showed a linear detection range of 0.0086-86 nM and a limit of detection of 7.7 pM, with specificity confirmed through testing against other proteins.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Wuzhen Qi, Siyuan Wang, Lei Wang, Xinge Xi, Shangyi Wu, Yanbin Li, Ming Liao, Jianhan Lin
Summary: A portable detection device was developed for rapid, sensitive and simple detection of viable Salmonella using a finger-actuated microfluidic chip and an improved recombinase aided amplification (RAA) assay. The device could distinguish viable bacteria from dead ones by combining improved propidium monoazide (PMAxx) with RAA. It could detect Salmonella typhimurium in spiked chicken meats from 1.3 x 10 2 CFU/mL to 1.3 x 10 7 CFU/mL in 2 hours with a lower detection limit of 130 CFU/mL, and showed potential for on-site detection of foodborne pathogens.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Shangyi Wu, Jing Yuan, Ai Xu, Lei Wang, Yanbin Li, Jianhan Lin, Xiqing Yue, Xinge Xi
Summary: In this study, a lab-on-a-tube biosensor was established for simple, rapid, sensitive, and specific detection of foodborne bacteria. The biosensor combined a rotatable Halbach cylinder magnet and an iron wire netting with magnetic silica beads for extraction and purification of DNA, and used recombinase-aided amplification (RAA) combined with a CRISPR-Cas12a assay for DNA amplification and fluorescence signal generation.
Article
Food Science & Technology
Li Xue, Fan Jiang, Xinge Xi, Yanbin Li, Jianhan Lin
Summary: This review focuses on the recent advances in microfluidic separators and biosensors for rapid and sensitive detection of foodborne pathogens. The key technologies for developing lab-on-chip separators and biosensors are illustrated, with particular emphasis on the strategy of lab-on-chip magnetic separation for continuous-flow isolation of pathogenic bacteria. The challenges and future perspectives for practical applications of lab-on-chip technologies in foods are also discussed.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Agricultural Engineering
X. Xi, R. Wang, P. Yao, L. Yao, S. Tung, Y. Li
TRANSACTIONS OF THE ASABE
(2020)
Article
Agricultural Engineering
P. Yao, R. Wang, X. Xi, Y. Li, S. Tung
TRANSACTIONS OF THE ASABE
(2019)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
