Review
Chemistry, Inorganic & Nuclear
Thavasilingam Nagendraraj, Sakthivel Vishnu Priya, Jamespandi Annaraj, Suresh Sagadevan
Summary: The primary biothiols, cysteine (Cys), glutathione (GSH), and homocysteine (Hcy), have crucial roles in various biological processes. Fluctuations in redox balance can lead to diseases due to disruptions in perfectly regulated intracellular and extracellular microenvironments. A study was conducted to develop copper-based fluorescence probes for detecting extracellular and intracellular targets such as cysteine (Cys) and glutathione (GSH) respectively. The fluorescence of the probes coordinated with Cu2+ is quenched by the binding of either Cys or GSH, resulting in retained Cu2+ ions.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Jingfeng Yao, Guoxing Yin, Ting Yu, Haitao Li, Peng Yin
Summary: A fluorescent probe has been developed that can simultaneously distinguish Cys/Hcy and GSH, and perform fluorescence imaging in live cells.
ANALYTICAL METHODS
(2021)
Article
Spectroscopy
Yi-Hang Sun, Hai-Hao Han, Jia-Min Huang, Jia Li, Yi Zang, Cheng-Yun Wang
Summary: DCIMA is a long-wavelength fluorescent probe for detecting Cys and GSH in living cells, with low detection limits and fast reaction speed, enabling sensitive imaging of biothiols located in lysosomes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Shaohui Han, Hui Zhang, Xiuxiu Yue, Jingpei Wang, Lei Yang, Benhua Wang, Xiangzhi Song
Summary: A ratiometric fluorescent probe CP was developed to selectively and rapidly discriminate between Cys and Hcy by introducing a phenylethynyl group. With distinct ratiometric fluorescence changes induced by the presence of Cys or Hcy, the probe was able to visualize and discriminate between intracellular Cys and Hcy in HeLa cells and zebrafish.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Zhaomin Wang, Yong Liu, Weishan Wang, Chang Zhao, Weiying Lin
Summary: PYQU is a fluorescent probe that can detect the interaction between RNA and SO2 in biological environments, showing changes in green and red fluorescence. It has low biological toxicity, good membrane permeability, and high selectivity, making it a valuable tool for studying RNA and SO2.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Spectroscopy
Xiuxiu Yue, Jiali Chen, Wenqiang Chen, Benhua Wang, Hui Zhang, Xiangzhi Song
Summary: The endoplasmic reticulum, known as the largest organelle in many eukaryotic cells, plays a vital role in maintaining normal cell function. A novel endoplasmic reticulum-targeting fluorescent probe, ER-CP, has been developed for distinguishing and imaging Cys, Hcy, and GSH with favorable sensitivity and selectivity. ER-CP shows good ER-targeting property and has been successfully applied in imaging these biothiols in HeLa cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Chemistry, Multidisciplinary
Xiaofeng Wu, Rui Wang, Nahyun Kwon, Huimin Ma, Juyoung Yoon
Summary: This article summarizes the methods for in situ imaging of enzymes using activatable fluorescent probes, and proposes that fluorescent probes with both activatable and in situ properties will facilitate high-resolution studies of enzymes in living systems.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Junyan Ma, Yaoyu Xu, Wen Zhao, Beibei Wang, Chunhuan Zhang, Zhenxing Zhang
Summary: Researchers have developed a Tag-Sec fluorescent probe that can detect both thioredoxin reductase and selenocysteine with high sensitivity and selectivity, along with broad pH tolerance and good water solubility. This concept may serve as a useful guide for designing probes for detecting Sec-containing proteins in clinical diagnosis.
MATERIALS CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Analytical
Xinmiao Lu, Muyu Wu, Siwen Wang, Jingcan Qin, Peiyong Li
Summary: In this study, a near infrared fluorescent probe (HI) was developed to detect and report intracellular cysteine and glutathione, and monitor the development of drug resistance in tumor. The probe showed strong near infrared fluorescence in response to cysteine and glutathione, with high specificity and no interference from other amino acids. Incubation with PATU-8988 and PATU-8988/Fu cells allowed for clear imaging and monitoring of intracellular cysteine and glutathione, while application to tumor-bearing mice facilitated monitoring and reporting of drug resistance.
Article
Chemistry, Analytical
Hui Wang, Hongtong Wang, Xiaoting Zhang, Mingyan Dong, Fang Huang, Ping Li, Bo Tang
Summary: This study successfully confirmed the decrease in mitochondrial Cys levels in mice with arthritis using a two-photon imaging probe, providing strong support for the development of new therapeutic approaches for related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Chen Xu, Yukun Zhang, Hui Sun, Jindong Ai, Mingguang Ren
Summary: We have developed a new two-photon fluorescent probe (T-HS) for the detection of H2S. The probe exhibits regular changes in absorption and fluorescence spectra upon addition of hydrogen sulfide. It demonstrates advantageous properties such as a large turn-on fluorescence signal, good selectivity, and low cytotoxicity. Furthermore, the probe T-HS has been successfully utilized for fluorescence imaging of H2S in live cells and zebrafish.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Yong-Peng Yang, Fu-Jian Qi, Ya-Long Zheng, De-Chen Duan, Xia-Zhen Bao, Fang Dai, Shengxiang Zhang, Bo Zhou
Summary: This study developed a fast imaging probe for mitochondrial TrxR2 and visualized the increased activity of TrxR2 in cancer cells, as well as the decreased activity in Parkinson's disease and ischemic stroke.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Xiaoqiang Tu, Lijun He, Haojie Huang, Haishun Ye, Lu Sun, Long Yi
Summary: Thiolysis of 7-cyanobenzoxadiazole (CBD) arylether was studied for the development of GSH-selective fluorescent probes, showing tunable reactivities and suitable dissociation constants for GSH. The results indicate that CBD-based probes are highly GSH-selective and suitable for bioimaging applications.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Xiaokun Yang, Jiamin Wang, Zunlong Zhang, Bo Zhang, Xiaolin Du, Jian Zhang, Jianhong Wang
Summary: In this study, a BODIPY-based turn-on fluorescent probe BDP-S was designed and synthesized for detecting the semi-essential amino acid cysteine (Cys). The probe exhibited fast response (10 min), distinct color change (from blue to pink), high signal-to-noise ratio (3150-fold), and high selectivity and sensitivity (LOD = 11.2 nM) towards Cys. Furthermore, BDP-S could be used for quantitative determination of Cys in food samples and qualitative detection of Cys on test strips. Importantly, BDP-S was successfully applied for imaging Cys in living cells and in vivo. Therefore, this work provides a potentially powerful tool for detecting Cys in food samples and complex biological systems.
Article
Chemistry, Analytical
Nahyun Kwon, Chang Su Lim, Gyeongju Ko, Jeongsun Ha, Dayoung Lee, Jun Yin, Hwan Myung Kim, Juyoung Yoon
Summary: A GSH-specific fluorescent probe targeting NMDA receptors with high selectivity was developed in this study, allowing for successful monitoring of GSH in neuronal cells and hippocampal tissues. This probe could potentially serve as a practical imaging tool to explore the function of GSH and related biological processes in the brain.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Susumu Yada, Ugis Lacis, Wouter van der Wijngaart, Fredrik Lundell, Gustav Amberg, Shervin Bagheri
Summary: This study investigates the impact of droplets on surfaces with inclined micropillars and discovers directional rebound at high impact speeds. The experimental observations are further supported by numerical simulations, revealing the detailed movement of the droplets over the pillars. These findings are significant for the design of structured surfaces to control droplet mobility.
Article
Chemistry, Multidisciplinary
Reza Zandi Shafagh, Sonia Youhanna, Jibbe Keulen, Joanne X. Shen, Nayere Taebnia, Lena C. Preiss, Kathrin Klein, Florian A. Buettner, Mikael Bergqvist, Wouter van der Wijngaart, Volker M. Lauschke
Summary: Aberrant glucose homeostasis is a common metabolic disturbance, but the prediabetic stage has received relatively little attention. Microphysiological tissue models provide opportunities to study metabolic interactions. A novel multitissue organ-on-a-chip device enables reciprocal communication between different human tissues, revealing the molecular networks underlying beta-cell stress in prediabetic hyperglycemia.
Article
Biochemistry & Molecular Biology
Linnea Gustafsson, Mathias Kvick, Carolina Astrand, Nienke Ponsteen, Nicolai Dorka, Veronika Hegrova, Sara Svanberg, Josef Horak, Ronnie Jansson, My Hedhammar, Wouter van der Wijngaart
Summary: This work demonstrates a method for producing suspended recombinant spider silk nanowires in solution. The method provides reproducibility, scalability, autoclavability, retention of bioactivity, and easy functionalization. The work also shows that coating a surface with nanowires can enhance the attachment and growth of human mesenchymal stem cells.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Kerem Kaya, Emre Iseri, Wouter van der Wijngaart
Summary: This study introduces easily programmable polymer magnetic composites with high remanence characteristics and the ability to reprogram between different states. The materials exhibit ferromagnetic properties and spin ice characteristics, and the magnetic properties can be controlled by temperature and magnetic field.
MICROSYSTEMS & NANOENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Hanie Kavand, Montse Visa, Martin Kohler, Wouter van der Wijngaart, Per-Olof Berggren, Anna Herland
Summary: Hybridizing biological cells with man-made sensors enables the accurate detection of weak physiological responses. Sutureless transplantation of biohybrid microstructures in the anterior chamber of the eye allows for long-term functionality studies and vascularization observations.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Filipe Marques, Wouter van der Wijngaart, Niclas Roxhed
Summary: This study aims to improve the safety of cell brushing in hard-to-reach locations by using brushes constructed from absorbable materials. The efficacy of loop brushes made of different types of absorbable suture wires, including PDS II, Monocryl, and Catgut, was investigated in an artificial cyst model alongside fine-needle aspiration. PDS II brushes demonstrated the highest efficiency, while Monocryl and Catgut also showed significant brushing effects. The efficiency of the brushes was inversely proportional to their plastic deformation by the needle. These results provide opportunities for safer cell biopsies in hard-to-reach locations through the use of brushes composed of absorbable materials.
BIOMEDICAL MICRODEVICES
(2023)
Article
Biochemical Research Methods
Kaiyang Zeng, Mohammad Osaid, Wouter van der Wijngaart
Summary: This study introduces a method for separating bacteria from blood by sedimentation velocity-based differentiation and size-based cross-flow filtration. The method can efficiently separate low concentration bacteria from blood.
Article
Chemistry, Multidisciplinary
Nikola Krivankova, Kerem Kaya, Wouter van der Wijngaart, Ulrica Edlund
Summary: Stimuli-responsive materials with reversible supramolecular networks controlled by a temperature change have attracted attention in various fields. In this study, the researchers investigated the polymerization of N-acryloyl glycinamide monomer using single-electron transfer reversible-deactivation radical polymerization, and studied its effect on the cloud point of the resulting polymers. They achieved a high conversion rate within a short period of time using a copper wire catalyst in water media. The synthesized PNAGA material exhibited a sharp phase transition, eliminating the need for inert conditions and deoxygenation.
Article
Engineering, Biomedical
Mikael Sandell, Arvin Chireh, Argyris Spyrou, Rikard Grankvist, Jonathan Al-Saadi, Stefan Jonsson, Wouter van der Wijngaart, Goran Stemme, Staffan Holmin, Niclas Roxhed
Summary: This study presents a micro-3D-printed device for harvesting endothelial cells for molecular analysis. In vivo evaluation in swine showed high selectivity towards endothelial cells and yielded tissue samples with gene expression signatures consistent with vascular tissue. Additionally, the device showed no short-term safety risks compared to traditional surgery.
ADVANCED NANOBIOMED RESEARCH
(2022)
Article
Biochemical Research Methods
Filipe Marques, Janosch Hauser, Emre Iseri, Igor Schliemann, Wouter van der Wijngaart, Niclas Roxhed
Summary: This study introduces a portable device for semi-automated sample preparation for rapid on-site evaluation (ROSE). By minimizing the equipment needed in the operating room, the device may simplify the performance of FNA sample preparation and lead to a wider implementation of ROSE.
Proceedings Paper
Engineering, Electrical & Electronic
Emre Iseri, Kerem Kaya, Rainer Heuchel, Wouter van der Wijngaart
Summary: Sliding microfluidic platforms allow multiplexed reactions in a single operation, but currently have limited capabilities. We propose a new three-plate sliding microfluidic platform that enables additional operations, expanding the range and complexity of applications. We demonstrate the capability to split bead solutions and perform viability assays on human cells.
2022 IEEE 35TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS CONFERENCE (MEMS)
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
Pegah Pezeshkpour, Wouter van der Wijngaart, Anna Herland
Summary: This study introduces a new method for microstructuring cell culture environments and successfully creates 3D periodic porous scaffolds. By preloading cells into hydrogels and culturing them in sugar solution before photostructuring, refractive index matching between the gel and cells was achieved, minimizing light scattering.
2021 21ST INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Weijin Guo, Jonas Hansson, Wouter van der Wijngaart
Summary: The method of measuring glucose concentration using synthetic paper and colorimetric assay achieved a low limit of detection and high accuracy, showing potential for clinical application.
2021 IEEE 16TH INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS (NEMS)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Weijin Guo, Jonas Hanson, Linnea Gustafsson, Wouter van der Wijngaart
Summary: In this study, polymer microstructures with inert surface finish were fabricated by folding and self-bonding using off-stoichiometric thiol-ene-epoxy (OSTE+) layers UV photocured resin. The folded structures were thermally cured to allow surfaces in contact with each other to spontaneously bond, stiffen the polymer, release folding stress, and consume reactive surface groups. The unique bend-and-bond technique was demonstrated in the creation of sample structures including a multilayer microfluidic chip with crossing channels, a helical spring, and a functional mini windmill, suggesting potential applications in biochip and biosensor fabrication.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Mikael Sandell, Stefan Jonsson, Wouter van Der Wijngaart, Goran Stemme, Staffan Holmin, Niclas Roxhed
Summary: The study introduces a new type of endovascular catheter for sampling of the blood vessel wall, designed to be non-invasive during axial motion and interact with the blood vessel wall when rotated. Initial results show successful in-vivo sampling of the wall of blood vessels less than 0.5 mm in diameter with minimal blood contamination.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
(2021)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
