Article
Chemistry, Multidisciplinary
Thithawat Trakoolwilaiwan, Yasuhiro Takeuchi, Terence S. Leung, Matej Sebek, Liudmyla Storozhuk, Linh Nguyen, Le Duc Tung, Nguyen Thi Kim Thanh
Summary: Dengue disease is a viral infection that has been widespread in tropical regions. Researchers developed a colorimetric thermal sensing LFA format for the detection of dengue virus NS1, which has the potential to enhance detection sensitivity and provide a visible signal without the need for an infrared camera.
Article
Immunology
Szu-Chia Lai, Yu-Yine Huang, Jiunn-Jye Wey, Meng-Hung Tsai, Yi-Ling Chen, Pei-Yun Shu, Shu-Fen Chang, Yi-Jen Hung, Jiu-Nan Hou, Chang-Chi Lin
Summary: Dengue is a rapidly spreading arboviral disease worldwide. A multiplex lateral flow immunoassay has been developed for the simultaneous detection of dengue viral NS1 antigen and identification of serotype. The proposed system provides high sensitivity and specificity and has important applications in detecting dengue virus serotypes and differentiating fever patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Indra Memdi Khoris, Fahmida Nasrin, Ankan Dutta Chowdhury, Enoch Y. Park
Summary: This study proposes a dual-approach aptasensor for highly sensitive early-stage diagnosis of dengue virus NS1 protein. The developed biosensor shows high sensitivity and good selectivity with colorimetric and electrochemical detection methods.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Cynthia Martinez-Liu, Carlos Machain-Williams, Natalia Martinez-Acuna, Sonia Lozano-Sepulveda, Kame Galan-Huerta, Daniel Arellanos-Soto, Mayra Melendez-Villanueva, Diana Avalos-Nolazco, Katya Perez-Ibarra, Sergio Galindo-Rodriguez, Aurora de Jesus Garza-Juarez, Ana Maria Rivas-Estilla
Summary: In this study, a biosensor based on gold nanoparticles and antibody was designed for the detection of dengue virus in vitro, showing significant differences in size and characteristics between different conjugates and naked AuNPs. The biosensor was further used in the development of a lateral flow assay for detecting flavivirus envelope protein in isolated DENV samples, with promising results for future dengue detection.
Article
Chemistry, Multidisciplinary
Zedong Li, Shuang Wu, Jingcheng Ji, Yuemeng Bai, Pengpeng Jia, Yan Gong, Shangsheng Feng, Fei Li
Summary: Lateral flow assays (LFAs), a popular point-of-care testing platform, have limited detection sensitivity. This study developed a ball pen writing-without-ink method to amplify the detection signal, resulting in improved sensitivity.
Article
Chemistry, Analytical
Ziting Ma, Jinnian Guo, Lu Jiang, Suqing Zhao
Summary: Dengue is a common and deadly mosquito-borne infectious disease globally. Early detection of dengue virus is crucial for preventing disease spread and providing timely response. Lateral flow immunoassay (LFIA) systems are low-cost, rapid, sensitive, and simple detection methods suitable for point-of-care testing in dengue-affected areas. However, current commercial LFIA kits fail to fully meet the requirements for sensitivity, specificity, serotype differentiation, and multiplex detection. Various strategies have been applied to optimize LFIA for dengue detection.
Article
Biophysics
Ye Tang, Hui Gao, Felix Kurth, Loic Burr, Konstantinos Petropoulos, Davide Migliorelli, Olivier T. Guenat, Silvia Generelli
Summary: LFIA is a well-established technique that provides immediate results, but suffers from high limits of detection. Introduction of a novel cellulose nanofiber aerogel material into LFIA strips has been shown to improve the detection sensitivity by extending the sample flow time.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Linxue Zhang, Shuang Chen, Rong Ma, Lijun Zhu, Ting Yan, Gulinigaer Alimu, Zhong Du, Nuernisha Alifu, Xueliang Zhang
Summary: Gold nanorods have been widely used in biomedical applications due to their adjustable optical and stable chemical properties, especially in the photothermal therapy of cancer. Surface modification can enhance the biocompatibility and stability of gold nanorods, making them more suitable for biomedical applications. The designed NIR-I-excitable photothermal theranostic nanoplatform Au@PEG NRs showed superior biocompatibility and chemical stability, making it promising for applications in cervical cancer treatment.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Analytical
Panagiotis Maglaras, Ioannis Lilis, Fotini Paliogianni, Vasiliki Bravou, Despina P. Kalogianni
Summary: A novel gold-nanoparticle-based nucleic acid lateral flow assay was developed for the rapid, visual, and quantitative detection of SARS-CoV-2. This method offers rapid, accurate, and cost-effective detection, making it a promising future diagnostic tool.
Article
Multidisciplinary Sciences
Charmainne Cruje, P. Joy Dunmore-Buyze, Eric Grolman, David W. Holdsworth, Elizabeth R. Gillies, Maria Drangova
Summary: This study demonstrates the use of PEG-coated gadolinium nanoparticles as a vascular contrast agent in micro-CT. Intravenous injection of the contrast agent in mice showed blood pool contrast enhancements of at least 200 HU for 30 min.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Trong-Nghia Le, Wesley Wei-Wen Hsiao, Yu-Yuan Cheng, Cheng-Chung Lee, Tan-Thanh Huynh, Dinh Minh Pham, Marvin Chen, Ming-Wei Jen, Huan-Cheng Chang, Wei-Hung Chiang
Summary: Dengue fever is a global viral infectious disease, and early detection is crucial for disease prevention and clinical care. In this study, a novel rapid and highly sensitive tool for dengue detection was developed, which can differentiate different NS1 protein serotypes, potentially enabling early detection in high-risk and limited-resource areas.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Zixuan Zhang, Wei Li, Jie Liu, Ying Li, Jingfu Qiu, Chaorui Li
Summary: This research proposes an electrochemical immunosensor based on the Al-TCPP adsorption aggregation signal amplification approach for the high sensitivity detection of the dengue virus NS1 protein. The immunosensor utilizes a metal-organic framework material and methylene blue to amplify the electrical signal. It exhibits good reproducibility and stability, with a wide detection range and low detection limit.
Article
Chemistry, Analytical
Qiang Fu, Lin Yuan, Fengrong Cao, Lihua Zang, Dandan Ji
Summary: The study developed a rapid, simple, and quantitative detection method for Salmonella using a novel biosensor, which can complete the testing in less than 40 minutes without the need for large laboratory equipment or analytical instruments.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Sian Sloan-Dennison, Emma O'Connor, James W. Dear, Duncan Graham, Karen Faulds
Summary: LFIA is vital for diagnosing biomolecules quickly at the POC, with SERS-based LFIA standing out as a method that enables identification and quantification of multiple targets simultaneously. Portable Raman instruments make it possible to conduct rapid and sensitive measurements at the POC, opening up new possibilities for healthcare in both urban and remote areas.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Nicolas Moser, Ling-Shan Yu, Jesus Rodriguez Manzano, Kenny Malpartida-Cardenas, Anselm Au, Paul Arkell, Chiara Cicatiello, Ahmad Moniri, Luca Miglietta, Wen-Hung Wang, Sheng Fan Wang, Alison Holmes, Yen-Hsu Chen, Pantelis Georgiou
Summary: In this study, a smartphone-connected handheld Lab-on-Chip platform was translated for quantitative detection of dengue virus. The platform utilizes microchip technology and a reverse-transcriptase loop mediated isothermal amplification assay to achieve rapid and accurate detection. The platform communicates with a smartphone app and cloud server for real-time synchronization, enabling epidemiological surveillance. This technology shows promise for clinical diagnosis in low-resource settings.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Wen-Rong Cai, Hai-Bo Zeng, Huai-Guo Xue, Robert S. Marks, Serge Cosnier, Xue-Ji Zhang, Dan Shan
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Tim Axelrod, Evgeni Eltzov, Robert S. Marks
Review
Chemistry, Analytical
Dorin Harpaz, Raymond C. S. Seet, Robert S. Marks, Alfred I. Y. Tok
Article
Biochemistry & Molecular Biology
Victor Markus, Orr Share, Kerem Terali, Nazmi Ozer, Robert S. Marks, Ariel Kushmaro, Karina Golberg
Article
Biochemistry & Molecular Biology
Victor Markus, Karina Golberg, Kerem Terali, Nazmi Ozer, Esti Kramarsky-Winter, Robert S. Marks, Ariel Kushmaro
Summary: Quorum sensing (QS) is a system of bacterial communication that regulates virulence depending on population density. In the case of multidrug-resistant bacteria like Pseudomonas aeruginosa, utilizing QS inhibitors such as furanone C-30 can effectively combat pathogenicity.
Article
Chemistry, Analytical
Junji Li, Wen-Li Xin, Yu-Xuan Dai, Guofang Shu, Xue-Ji Zhang, Robert S. Marks, Serge Cosnier, Dan Shan
Summary: Metal-organic frameworks (MOFs) are versatile coordinators for synthesizing high-performance catalysts, such as the redox-alternating CuxO electrochemical catalyst. The study synthesized a novel precursor MOF and characterized the transformation towards a highly active nanocomposite through temperature modulation to enhance sensitivity in H2O2 reduction. The resulting sensor material exhibited amplified sensitivity and ease of preparation, suggesting wide applications in medical settings.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Victor Markus, Orr Share, Marilou Shagan, Barak Halpern, Tal Bar, Esti Kramarsky-Winter, Kerem Terali, Nazmi Ozer, Robert S. Marks, Ariel Kushmaro, Karina Golberg
Summary: The study found that artificial sweeteners such as aspartame, sucralose, and saccharin inhibit the communication system of Gram-negative bacteria AHL, possibly affecting the balance of gut microbial community by interfering with protein folding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Li-Ping Zong, Junji Li, Guofang Shu, Xinye Liu, Robert S. Marks, Xue-Ji Zhang, Serge Cosnier, Dan Shan
Summary: A novel ECL amplification strategy was established by designing Fe-N-C nanosheets with exceptional catalytic activity for O-2 activation. The material was successfully applied for antibiotic detection, indicating promising prospects for biological analysis and environmental monitoring.
ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Karina Golberg, Victor Markus, Bat-el Kagan, Sigalit Barzanizan, Karin Yaniv, Kerem Terali, Esti Kramarsky-Winter, Robert S. Marks, Ariel Kushmaro
Summary: Antimicrobial resistance, particularly antibacterial resistance, is a major global health problem. Current treatments for bacterial diseases are ineffective due to protective and resistant biofilm structures. This study investigates the anti-virulence activity of 3,3'-diindolylmethane (DIM), a phytochemical, and finds that it has anti-biofilm activity against major Gram-negative pathogens. Combining DIM with an antibiotic enhances its inhibition of biofilm formation. The study also reveals the molecular mechanisms underlying bacterial response to DIM, providing evidence for its potential in attenuating bacterial virulence.
Article
Chemistry, Multidisciplinary
Li-Ping Zong, Xiaozhong Chen, Dunru Zhu, Xi-Jie Li, Feng Li, Serge Cosnier, Xue-Ji Zhang, Robert S. Marks, Dan Shan
Summary: A novel electrochemiluminescence amplification strategy was developed using macrocyclic Schiff base Fe complexes, which showed remarkable redox electrocatalytic properties and enhanced ECL efficiency. The strategy was successfully applied in the detection of neomycin, highlighting its potential in biological analysis and environmental monitoring.
Review
Biochemistry & Molecular Biology
Victor Markus, Abraham Abbey Paul, Kerem Terali, Nazmi Ozer, Robert S. Marks, Karina Golberg, Ariel Kushmaro
Summary: An imbalance in gut microbiota, called dysbiosis, can affect host health and lead to diseases like inflammatory bowel disease, cancer, obesity, depression, and autism. The inhibition of bacterial quorum sensing (QS) by artificial sweeteners has been found to contribute to this dysbiosis. QS is a communication system mediated by small molecules, called autoinducers (AIs), which enable bacteria to interact and coordinate their gene expression. AIs play a role in maintaining the equilibrium of gut microbiota and interference in QS can disrupt this balance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Yifat Baruch, Karina Golberg, Qun Sun, Karina Yew-Hoong Gin, Robert S. Marks, Ariel Kushmaro
Summary: This study tested the effect of a natural compound called 3,3'-Diindolylmethane (DIM) on the biofilm of the oral bacterium Streptococcus mutans. The results showed that DIM was able to reduce the formation of S. mutans biofilm and decrease its durability under acidic conditions. This suggests that DIM has potential in reducing biofilm formation and preventing dental caries.
Article
Virology
Michal Liddor Naim, Yu Fu, Marilou Shagan, Itay Bar-Or, Robert Marks, Qun Sun, Rony Granek, Ariel Kushmaro
Summary: The COVID-19 pandemic has caused significant mortality and morbidity worldwide. Continuous virus mutations have led to the emergence of new variants. This study demonstrates the potential of wastewater-based epidemiology as a valuable tool for future pandemics, as it enables early detection of potential outbreaks and provides insights into variant dynamics.
Article
Chemistry, Analytical
Yi-Xuan Li, Junji Li, Wen-Rong Cai, Wen-Li Xin, Robert S. Marks, Hai-Bo Zeng, Serge Cosnier, Xueji Zhang, Dan Shan
ANALYTICAL CHEMISTRY
(2020)
Article
Microbiology
Dorin Harpaz, Robert S. Marks, Ariel Kushmaro, Evgeni Eltzov
FUTURE MICROBIOLOGY
(2020)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)