4.7 Article

A low cost and portable smartphone microscopic device for cell counting

期刊

SENSORS AND ACTUATORS A-PHYSICAL
卷 274, 期 -, 页码 57-63

出版社

ELSEVIER SCIENCE SA
DOI: 10.1016/j.sna.2018.03.009

关键词

Microscopic; Smartp hone; Portable; Cell counting; Low cost

资金

  1. National Natural Science Foundation of China [51505123, 51728502, 51605136, 11647121]
  2. China Postdoctoral Science Foundation [20177100149, 2015M580190, 2016M600180]
  3. Provincial Natural and Science Foundation of Hebei [E2015202194, C2017202208]
  4. Hebei Research Foundation for Outstanding Young Talents [BJ2014014]
  5. Hebei Provincial Science and Technology Foundation [15271704]
  6. Starting Foundation of HEBUT [208003]
  7. Foundation for the Science and Technology Program of Higher Education Institutions of Hebei Province, China [QN2016113]
  8. College Students Innovation and Entrepreneurship Training Program of HEBUT [201710080009]
  9. Scientific Innovation Grant for Excellent Young Scientists of Hebei University of Technology, China [2015010]

向作者/读者索取更多资源

Portable smartphone microscopic devices can potentially address various imaging and cell counting needs in healthcare monitoring. In this paper, a portable microscopic platform is constructed by a commercial smartphone and a custom-designed slider-scale microfluidic chip. Here the smartphone acts both as a detector and image analysis station. We demonstrate the adaptation of a smartphone's camera by loading a single-ball lens to function as a compact microscope, and then a glass slider or a slider-scale multiplex microfluidic chip is designed for preprocess of the samples. Our portable imaging device allows for micron size resolution and a home-coded Android application is performed for automatic/manual particle counting. Pumpkin pollen powders on a slider were counted as a demo test and then the counting for dairy somatic cells within a microfluidic chip were carried out automatically. The results from the smartphone microscopic device show good agreement with those values done by commercial microscope and flowcytometry. With advances in miniaturized electronics and smartphones, this low cost and portable smartphone microscopic platform can be used as common and convenient tools to perform true point-of-care biomedical or biochemical lab-on-a-chip applications. (C) 2018 Elsevier B.V. All rights reserved.

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