期刊
SENSORS
卷 18, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/s18041173
关键词
dengue virus detection; nickel(II) salphen complex; optical DNA biosensor; porous silica nanospheres; reflectance measurement; synthetic DNA binder
资金
- Malaysian Ministry of Science, Technology and Innovation through E-Science Fund [06-01-02-SF1242]
- Nanotechnology Directorate Grant [NND/ND/(2)/TD11-009]
- National University of Malaysia (UKM) via 'Dana Impak Perdana' research grant [DIP-2016-028]
A sensitive and selective optical DNA biosensor was developed for dengue virus detection based on novel square-planar piperidine side chain-functionalized N,N'-bis-4-(hydroxysalicylidene)-phenylenediamine-nickel(II), which was able to intercalate via nucleobase stacking within DNA and be functionalized as an optical DNA hybridization marker. 3-Aminopropyltriethoxysilane (APTS)-modified porous silica nanospheres (PSiNs), was synthesized with a facile mini-emulsion method to act as a high capacity DNA carrier matrix. The Schiff base salphen complexes-labelled probe to target nucleic acid on the PSiNs renders a colour change of the DNA biosensor to a yellow background colour, which could be quantified via a reflectance transduction method. The reflectometric DNA biosensor demonstrated a wide linear response range to target DNA over the concentration range of 1.0 x 10(-16)-1.0 x 10(-10) M (R-2 = 0.9879) with an ultralow limit of detection (LOD) at 0.2 aM. The optical DNA biosensor response was stable and maintainable at 92.8% of its initial response for up to seven days of storage duration with a response time of 90 min. The reflectance DNA biosensor obtained promising recovery values of close to 100% for the detection of spiked synthetic dengue virus serotypes 2 (DENV-2) DNA concentration in non-invasive human samples, indicating the high accuracy of the proposed DNA analytical method for early diagnosis of all potential infectious diseases or pathological genotypes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据