Article
Biology
Kai Sun, Chun Wang, Jianqiu Xiao, Michael D. Brodt, Luorongxin Yuan, Tong Yang, Yael Alippe, Huimin Hu, Dingjun Hao, Yousef Abu-Amer, Matthew J. Silva, Jie Shen, Gabriel Mbalaviele
Summary: Amino-terminal fragments of proteolytically cleaved GSDMs form plasma membrane pores, leading to pyroptosis. GSDMD and GSDME play roles in bone microenvironment and contribute to fracture healing. Both GSDMD and GSDME regulate inflammatory responses through the GSDM-IL-1 axis, promoting bone healing after fracture.
Article
Surgery
Hassan ElHawary, Aslan Baradaran, Jad Abi-Rafeh, Joshua Vorstenbosch, Liqin Xu, Johnny Ionut Efanov
Summary: Bones make up a significant percentage of human weight and play important physiologic and structural roles. Bone remodeling involves renewing healthy bone to maintain bone strength and calcium and phosphate balance through four phases. Inflammation plays a crucial role in both bone remodeling and healing.
SEMINARS IN PLASTIC SURGERY
(2021)
Article
Immunology
Yasmine Hachemi, Anna E. Rapp, Sooyeon Lee, Ann-Kristin Dorn, Benjamin T. Krueger, Kathrin Kaiser, Anita Ignatius, Jan Tuckermann
Summary: Effective GR dimerization plays a role in trauma-induced compromised fracture healing, as revealed by a study on mice with decreased GR dimerization ability. GR(dim) mice were found to be protected from the impairment of fracture healing caused by additional thorax trauma.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Baixue Xiao, Yuxuan Liu, Indika Chandrasiri, Emmanuela Adjei-Sowah, Jared Mereness, Ming Yan, Danielle S. W. Benoit
Summary: This study developed a bone-targeted nanoparticle drug delivery system that enhances fracture healing by modulating macrophage polarization. The experimental results showed that the system is predominantly uptaken by fracture-associated macrophages, resulting in increased M2/M1 ratio and expedited fracture healing, accompanied by upregulation of anti-inflammatory genes and downregulation of pro-inflammatory genes.
Article
Immunology
Hyuk-Kwon Kwon, Sean V. Cahill, Kristin E. Yu, Kareme D. Alder, Christopher M. Dussik, Jain Jeong, Jung Ho Back, Francis Y. Lee
Summary: The study suggests that teriparatide may be a viable adjunct therapeutic agent to improve bony union and bone microarchitecture and prevent the development of septic nonunion under diabetic conditions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Orthopedics
Justin E. Hellwinkel, Zachary M. Working, Laura Certain, Andres J. Garcia, Joseph C. Wenke, Chelsea S. Bahney
Summary: Infection is a common cause of impaired fracture healing, and the current approach of treating fractures and infections separately can be costly and result in more procedures and patient morbidity. Bringing together scientists and engineers to develop new therapies that address both infection and fracture healing is essential. This article highlights the need for collaboration and research into the challenges posed by delayed fracture healing due to infection.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Biology
Dominik Saul, David G. Monroe, Jennifer L. Rowsey, Robyn Laura Kosinsky, Stephanie J. Vos, Madison L. Doolittle, Joshua N. Farr, Sundeep Khosla
Summary: Senescent cells and senescence-related secretory phenotype markers increase during fracture healing. Intermittent treatment with senolytics reduces senescence markers in the fracture callus and accelerates the time course of fracture healing.
Article
Materials Science, Multidisciplinary
Yan Peng, Huaizhi Liu, Hao Peng, Jiuyang Zhang
Summary: Self-healing materials have long struggled to achieve both high mechanical strength and self-healing ability. This work successfully achieves self-repair in mechanically robust materials, inspired by biological self-healing. The mechanical performance and self-healing behavior of these materials can be precisely controlled through temperature and composition. An artificial bone-tissue system is created with bionic structure, effective self-healing, and high modulus.
Article
Computer Science, Interdisciplinary Applications
Enhao Zhang, Saeed Miramini, Minoo Patel, Martin Richardson, Peter Ebeling, Lihai Zhang
Summary: The study found that there is a significant increase in TNF-alpha level in the fracture callus within the first day post-fracture under normal conditions, and its influence on the concentration of MSCs and cell differentiation becomes significant three days post-fracture. Excessive TNF-alpha secretion induced by diabetes could decrease the concentration of MSCs in the initial stage of healing.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2022)
Article
Multidisciplinary Sciences
Jana Schreier, Silke Rautenschlein, Arne Jung
Summary: This study demonstrates the differences in virulence among Enterococcus cecorum strains in experimentally infected chickens, highlighting the importance of further characterizing virulence factors and pathogenic mechanisms of EC. The EC14 strain at a dose of 10(6) CFU is suitable for reproducing the EC-associated disease in broiler chickens, providing a foundation for future research on EC pathogenesis and potential prevention and intervention strategies.
Review
Endocrinology & Metabolism
Dominik Saul, Sundeep Khosla
Summary: More than 2.1 million age-related fractures occur in the United States annually, resulting in a significant socioeconomic burden. Age-related deterioration of bone structure is associated with impaired bone healing, which occurs in four stages: inflammation, angiogenesis and cartilage formation, soft callus development, and remodeling. Impaired fracture healing due to aging is related to detrimental changes at the cellular level, and recent studies show that clearing senescent cells enhances fracture repair.
Article
Biochemistry & Molecular Biology
Silvia Concetta Colucci, Cinzia Buccoliero, Lorenzo Sanesi, Mariella Errede, Graziana Colaianni, Tiziana Annese, Mohd Parvez Khan, Roberta Zerlotin, Manuela Dicarlo, Ernestina Schipani, Kenneth M. Kozloff, Maria Grano
Summary: The study found that irisin promotes bone formation and accelerates the fracture healing process, suggesting its potential use as a novel pharmacologic modulator.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Chul-Ho Kim, Dong Hwan Kim, Han-Vit Kang, Won Jun Kim, Minkyu Shin, Ji Wan Kim
Summary: Percutaneous intramedullary nailing is an effective technique for metacarpal fractures, but the fracture site and number of K-wires used can affect the time to achieve bone union.
Article
Multidisciplinary Sciences
Cassandra Conceicao, Antonio Completo, Marco Soares P. dos Santos
Summary: Bone fractures are a global health issue, and current imaging-based monitoring techniques are subjective, expensive, and unable to provide daily monitoring of fracture healing stages. The development of reliable and non-invasive technologies is essential to minimize healing risks.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Multidisciplinary Sciences
Lili Borbala Flink, Ameneh Ghaffarinia, Benjamin Tamas Papp, Akos Varga, Andras Istvan Vigh, Daniel Laszlo Vidacs, Robert Kui, Lajos Kemeny, Zsuzsanna Bata-Csorgo, Renata Bozo
Summary: Psoriatic skin shows abnormal wound healing-like characteristics, even in non-lesional skin. Elevated levels of fibroblast-derived dermal periostin were observed in psoriatic patients, especially in those receiving systemic treatment. Basal keratinocytes in psoriatic skin exhibited significant periostin positivity, both in non-lesional, lesional, and previously-lesional areas. This abnormal wound healing-like phenotype with overexpression of periostin in psoriatic skin suggests a potential compensatory mechanism initiated by basal membrane injury, which can persist in healed skin.
SCIENTIFIC REPORTS
(2023)
Article
Orthopedics
Andreas M. Seitz, Janina Leiprecht, Jonas Schwer, Anita Ignatius, Heiko Reichel, Thomas Kappe
Summary: The purpose of this study was to investigate the potential of a doubled semitendinosus (ST) and a single gracilis tendon (GT) lateral meniscus autograft to restore knee joint kinematics and tibiofemoral contact after total lateral meniscectomy (LMM). The results showed that the ST autograft significantly improved knee joint kinematics and restored tibiofemoral contact mechanics, while the GT autograft had limited effectiveness.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Immunology
Melanie Haffner-Luntzer, Birte Weber, Kazuhito Morioka, Ina Lackner, Verena Fischer, Chelsea Bahney, Anita Ignatius, Miriam Kalbitz, Ralph Marcucio, Theodore Miclau
Summary: Clinical and preclinical data suggest accelerated bone fracture healing in subjects with an additional traumatic brain injury (TBI). Mechanistically, altered metabolism and neuro-endocrine regulations have been shown to influence bone formation after combined fracture and TBI, thereby increasing the bone content in the fracture callus. However, the early inflammatory response towards fracture and TBI has not been investigated in detail so far.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jana Riegger, Helga Joos, Valentin Moehler, Frank Leucht, Katrin Rading, Christian Kubisch, Anita Ignatius, Markus Huber-Lang, Rolf E. Brenner
Summary: The study suggests that the terminal complement complex (TCC) plays a role in the pathogenesis of posttraumatic osteoarthritis (PTOA). However, the absence of TCC may protect against OA progression and have minor effects on the micro-structure of the subchondral bone.
Article
Endocrinology & Metabolism
Mubashir Ahmad, Nadine Stirmlinger, Irfana Jan, Ulrich Stifel, Sooyeon Lee, Marcel Weingandt, Ulrike Kelp, Juergen Bockmann, Anita Ignatius, Tobias M. Boeckers, Jan Tuckermann
Summary: Mutations in the postsynaptic scaffold protein Shank2 are associated with autism spectrum disorders (ASD) and an increased risk of fractures in affected patients. This study investigates the direct role of Shank2 in regulating bone mass. The findings indicate that Shank2 is expressed in bone and plays a crucial role in osteoblast differentiation and bone formation. The interaction between Shank2 and proteins known from the central nervous system is identified as important for osteoblast differentiation.
Article
Biochemistry & Molecular Biology
Franziska Widmayer, Cornelia Neidlinger-Wilke, Fiona Witz, Jan U. Jansen, Anita Ignatius, Melanie Haffner-Luntzer, Graciosa Q. Teixeira
Summary: Postmenopausal women have an increased risk of intervertebral disc degeneration, possibly due to decreased estrogen levels. This study investigated the effects of 17-beta-estradiol (E2) and low-magnitude, high-frequency vibration (LMHFV) on intervertebral discs (IVDs). The results showed that E2 treatment had minor effects on gene expression, cellularity, and matrix metabolism, while LMHFV alone led to cell loss and increased production of IL-6. However, the combination of E2 and LMHFV had a protective effect against cell loss and decreased IL-6 production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alessandro Alan Porporati, Yvonne Modinger, Sarah Fischer, Sara Polajzer, Melanie Mettang, Ulrike Deisinger, Matejka Podlogar, Rihard Trebse, Nika Lovsin
Summary: Ten percent of patients undergoing total hip arthroplasty (THA) require revision surgery. The study compared the toxicity and immunogenicity of nanoparticles from Al2O3, zirconia-toughened alumina (ZTA), and cobalt chrome (CoCr) in THP-1 macrophage cell line and human primary macrophages. Results showed that ZTA is a non-toxic implant material with no immunogenic effects in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Verena Fischer, Jasmin Maria Buelow, Benjamin Thilo Krueger, Deniz Ragipoglu, Anna Vikman, Melanie Haffner-Luntzer, Konstantinos Katsoulis-Dimitriou, Anne Dudeck, Anita Ignatius
Summary: Previous studies have shown that an increase in mast cells in the bone marrow is associated with age-related and post-menopausal osteoporosis. However, this study found that mast cell-derived RANKL does not contribute to the development of post-menopausal osteoporosis, suggesting that other factors may be responsible for this condition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Melanie Haffner-Luntzer, Deniz Ragipoglu, Mubashir Ahmad, Astrid Schoppa, Lena Steppe, Verena Fischer, Julia Luther, Timur Yorgan, Ernesto Bockamp, Michael Amling, Thorsten Schinke, Anita Ignatius
Summary: Despite improvements in fracture care, a percentage of fractures still heal poorly. The study investigated the potential of Wnt1 as a molecule to improve bone fracture healing. The results showed that Wnt1 promotes bone formation during fracture healing through YAP/BMP signaling. This suggests that Wnt1 could be used as a therapeutic agent for orthopedic complications.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Review
Endocrinology & Metabolism
Merle Stein, Florent Elefteriou, Bjorn Busse, Imke A. K. Fiedler, Ronald Young Kwon, Eric Farrell, Mubashir Ahmad, Anita Ignatius, Liam Grover, Liesbet Geris, Jan Tuckermann
Summary: Major achievements in bone research have relied on animal models and in vitro systems. However, the ethical debate surrounding animal experimentation and advancements in in vitro and in silico techniques have challenged their use. This review provides an overview of the strengths and limitations of animal models, as well as in vitro and in silico technologies, in skeletal research. The authors propose that a combination of the right animal model and state-of-the-art technology is essential for advancing bone research.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Engineering, Biomedical
Lena Schroeter, Friederike Kaiser, Anna-Lena Preissler, Philipp Wohlfahrt, Oliver Kueppers, Uwe Gbureck, Anita Ignatius
Summary: To address the challenges of time-consuming and error-prone preparation of hydroxyapatite (HA) cements for bone defect treatment, researchers have developed a prefabricated magnesium phosphate cement paste based on glycerol. This ready-to-use paste can be directly applied during surgery and shows a promising degradation rate and support for bone regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Orthopedics
Matthias Sukopp, Maoz Shemesh, Elena Pruech, Eran Linder-Ganz, Scott Hacker, Vincenzo Condello, Jonas Schwer, Anita Ignatius, Lutz Duerselen, Andreas Martin Seitz
Summary: The purpose of this in-vitro study was to examine the kinematics of an artificial, free-floating medial meniscus replacement device under dynamic loading situations and different knee joint states. A dynamic knee simulator was used to perform dynamic loading exercises on left human cadaveric knee joints to investigate the knee joint and implant kinematics. The results showed that the non-anchored free-floating device remains within the medial knee joint gap under challenging dynamic loading situations without indicating any luxation tendencies.
JOURNAL OF EXPERIMENTAL ORTHOPAEDICS
(2023)
Article
Endocrinology & Metabolism
Lena Steppe, Benjamin Krueger, Miriam Eva Angelica Tschaffon-Mueller, Jan-Moritz Ramge, Astrid Schoppa, Anita Ignatius, Melanie Haffner-Luntzer
Summary: External mechanostimulation induced by LMHFV has different effects on fracture healing in estrogen-competent and estrogen-deficient rodents. ER alpha signaling in osteoblasts is required for these effects, with ligand-dependent and -independent ER alpha signaling playing different roles. The AF-2 domain of the ER alpha receptor is crucial for the negative effects of vibration during bone fracture healing in estrogen-competent mice, suggesting that the osteoanabolic effects of vibration are mediated by ligand-independent ER alpha signaling.
Article
Orthopedics
Luisa de Roy, Kerstin Eichhorn, Martin Faschingbauer, Klaus Schlickenrieder, Anita Ignatius, Andreas Martin Seitz
Summary: The purpose of this in vitro study was to investigate the effect of hyaluronic acid supplementation on knee joint friction during osteoarthritis progression. The results showed that knee joint friction does not increase with degeneration, and hyaluronic acid supplementation does not lead to an initial decrease in friction.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Meeting Abstract
Dermatology
P. Maity, K. Singh, L. Krug, A. Koroma, W. Bloch, S. Kochanek, M. Wlaschek, M. Schorpp-Kistner, P. Angel, A. Ignatius, H. Geiger, K. Scharffetter-Kochanek
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jana Riegger, Astrid Schoppa, Leonie Ruths, Melanie Haffner-Luntzer, Anita Ignatius
Summary: This article discusses oxidative stress as a major driver of pathophysiological processes in cartilage and bone, including aging, misdirected differentiation, cell death, mitochondrial dysfunction, and impaired mitophagy. It also elaborates on cellular defense mechanisms and therapeutic strategies to improve cell and tissue protection.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)