期刊
REPRODUCTIVE TOXICOLOGY
卷 77, 期 -, 页码 53-61出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2018.01.008
关键词
Valproic acid (VPA); RNA sequencing (RNA-seq); Transcriptome; Alternative splicing gene; Autism
资金
- National Natural Science Foundation of China [31371501]
- Natural Science Foundation of Shaanxi Province [2017JM8047]
- Fundamental Research Funds for the Central Universities [GK201702006, 2016CSY016]
- National Undergraduate Scientific and Technological Innovation Project [201610718020]
- Xi'an Medical University [2016PT06, 2017GJFY25]
- Training Program of Interdisciplinary Studies of Learning Science, Shaanxi Normal University
Gene-environmental interaction could be the major cause of autism. The aim of the current study is to detect the effects of valproic acid on gene expression profiles and alternatively spliced genes in the pre-frontal cortex in rat models of autism. Female rats received a single intraperitoneal injection of 600 mg/kg valproic acid at day 12.5 post-conception, and controls were injected with saline. Only male offspring were employed in the current study. RNA sequencing was used to investigate transcriptome in the prefrontal cortex of VPA-exposed rats. There were 3228 differently expressed genes and 637 alternative spliced genes, in VPA rats compared to controls. Pathways enrichment among the differently expressed genes and alternatively spliced genes were associated with neurological diseases and neural system development. The results implied VPA affected transcriptional and splicing events genome-wide and the transcriptional and splicing events may be associated with the autistic behaviors of VPA rats. (C) 2018 Elsevier Inc. All rights reserved.
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