期刊
PROTOPLASMA
卷 255, 期 5, 页码 1295-1307出版社
SPRINGER WIEN
DOI: 10.1007/s00709-018-1227-z
关键词
Hydrogen peroxide; Nitric oxide; Polyamines; Root growth; Antioxidant enzymes
资金
- Universidad de Buenos Aires, Argentina [UBACYT 20020130100178BA]
- Consejo Nacional de Investigaciones Cientificas y Tecnicas [PIP 266]
- ANPCyT (Agencia Nacional de Promocion Cientifica y Tecnologica, Argentina)
Polyamines (PAs) produce H2O2 and nitric oxide (NO) during their normal catabolism and modulate plant growth and development. To explore the biochemical basis of PAs-induced growth inhibition in Triticum aestivum L seedlings, we examined the role of O-2(center dot-) , H2O2 or NO in shoot and root development. Although all PA treatments resulted in a variable reduction of root and shoot elongation, spermine (Spm) caused the greater inhibition in a similar way to that observed with the NO donor, sodium nitroprusside (SNP). In both cases, O-2(center dot-) production was completely blocked whereas H2O2 formation was high in the root apex under SNP or Spm treatments. Catalase recovered root and shoot growth in SNP but not in Spm-treated plants, revealing the involvement of H2O2 in SNP-root length reduction. The addition of the NO scavenger, cPTIO, restored root length in SNP- or Spm-treated plants, respectively, and partially recovered O(2)(center dot-)levels, compared to the plants exposed to PAs or SNP without cPTIO. A strong correlation was observed between root growth restoration and O-2(center dot-) accumulation after treating roots with SNP + aminoguanidine, a diamine oxidase inhibitor, and with SNP + 1,8-diaminoctane, a polyamine oxidase inhibitor, confirming the essential role of O-2(center dot-) formation for root growth and the importance of the origin and level of H2O2 . The differential modulation of wheat growth by PAs through reactive oxygen species or NO is discussed.
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