期刊
PLOS ONE
卷 13, 期 5, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0197439
关键词
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资金
- National Institutes of Health (NIH) Loan Repayment Program
- Eunice Kennedy Shriver National Institute Of Child Health & Human Development (NICHD) [T32HD068256]
- National Institute Of Diabetes and Digestive Kidney Diseases (NIDDK) [T32DK007673, K08DK090146]
- National Institute of Allergy and Infectious Diseases (NIAID) [F32FAI120553]
- Vanderbilt Diabetes Center [P30DK20593]
- NICHD [R01 HD081121, K08HD061607]
- Investigators in the Pathogenesis of Infectious Disease grant from the Burroughs Wellcome Fund
- Global Alliance to Prevent Prematurity and Stillbirth (GAPPS)
- Emch Foundation
- CD Foundation
- Institutional Research Training Award [T32 AI007180]
Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it's taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined the presence of bacterial DNA in the intestines and placentas of fetal mice at gestational day 17 (n = 13). These were compared to newborn intestines (n = 15), maternal sites (mouth, n = 6; vagina, n = 6; colon, n = 7; feces, n = 8), and negative controls to rule out contamination. The V4 region of the bacterial 16S rRNA gene indicated a pattern of bacterial DNA in fetal intestine similar to placenta but with higher phylogenetic diversity than placenta or newborn intestine. Firmicutes were the most frequently assignable phylum. SourceTracker analysis suggested the placenta as the most commonly identifiable origin for fetal bacterial DNA, but also over 75% of fetal gut genera overlapped with maternal oral and vaginal taxa but not with maternal or newborn feces. These data provide evidence for the presence of bacterial DNA in the mouse fetus.
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