Article
Biotechnology & Applied Microbiology
Julian Wichmann, Annibel Eggert, Liam D. H. Elbourne, Ian T. Paulsen, Kyle J. Lauersen, Olaf Kruse
Summary: In this study, the accessibility of different isoprenoid precursor pools for sesquiterpenoid production in Chlamydomonas reinhardtii was investigated. It was found that both the cytoplasm and chloroplast can be utilized for the generation of sesquiterpenoids. Targeting the fusion protein to the chloroplast and increasing gene dosage significantly improved sesquiterpene production. In addition, potential prenyl unit transporters were proposed based on bioinformatic analyses, which could enhance the capabilities of sesquiterpenoid production.
MICROBIAL CELL FACTORIES
(2022)
Article
Agricultural Engineering
Jia-Yi Lin, Sefli Sri Wahyu Effendi, I-Son Ng
Summary: This study explores the use of genetic modification to enhance CO2 capture and utilization in microalgae for increased biomass and product production. The results show that genetically modified C. reinhardtii can achieve maximum biomass, lutein, and lipid production under autotrophic conditions in a photo-bioreactor with 5% CO2, with improved CO2 assimilation rates.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Huilin Zhuang, Yaohui Ou, Ruoyu Chen, Danqiong Huang, Chaogang Wang
Summary: In this study, the ALFPm3 peptide was successfully expressed and secreted in C. reinhardtii using the fusion of signal peptides ARS1 and CAH1. The ALFPm3 extracts from the culture media showed significant inhibitory effects on the growth of Vibrio species, with the CAH1 signal peptide showing a higher inhibitory rate compared to the ARS1 signal peptide. This research provides a new strategy for the secretory production of ALFPm3 with high antibacterial activity in C. reinhardtii, enhancing its potential application in the aquaculture industry.
Article
Agricultural Engineering
Jia-Yi Lin, I-Son Ng
Summary: This study used genetic engineering to enhance the growth, lipid and lutein production of Chlamydomonas reinhardtii. CRISPRi was applied to down-regulate the phosphoenolpyruvate carboxylase (PEPC1) gene, resulting in increased carbon flux towards lipid synthesis. The engineered strain PGi showed the highest biomass, lipid and lutein production at 35°C.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Amina Antonacci, Ivo Bertalan, Maria Teresa Giardi, Viviana Scognamiglio, Mehmet Turemis, Dirk Fisher, Udo Johanningmeier
Summary: This study successfully designed novel mutant strains of Chlamydomonas reinhardtii to produce bioactive peptides with high antioxidant capacity. The transgenic algae showed high in vivo antioxidant activity towards radical oxygen-generating substances, longer shelf-life, and better resistance to high light and heat stress. The mutant strains have great potential as robust and sensitive biological recognition elements for bioassay applications, such as herbicide detection using an optical bioassay.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Carolina Ehrenfeld, Valentina Veloso-Gimenez, Rocio Corrales-Orovio, Rolando Rebolledo, Mauricio P. Boric, Jose Tomas Egana
Summary: As animal cells cannot produce oxygen, erythrocytes are responsible for gas interchange, being able to capture and deliver oxygen upon tissue request. Several other cells in nature produce oxygen by photosynthesis, raising the question of whether they could circulate within the vascular networks, acting as an alternative source for oxygen delivery. This study examines the physical and mechanical features of the photosynthetic microalga Chlamydomona reinhardtii and compares them with erythrocytes, finding similarities in size and rheological properties. Furthermore, the biocompatibility of the microalgae is evaluated, showing that they can co-culture with endothelial cells without affecting each other's morphology and viability. Additionally, the systemic injection of high numbers of microalgae in mice does not trigger harmful responses, suggesting their potential as a source of oxygen delivery.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mei-Li Zhao, Xiang-Yu Li, Cheng-Xiang Lan, Zi-Ling Yuan, Jia-Lin Zhao, Ying Huang, Zhang-Li Hu, Bin Jia
Summary: A synthetic pathway for ginsenosides was established in Chlamydomonas reinhardtii by introducing genes from Panax ginseng, resulting in high production of dammarenediol-II. Strategies such as gene loading and culture optimizing were employed to enhance productivity. This study provides a good platform for the further production of ginsenosides in microalgae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Mei-Li Zhao, Wen-Sheng Cai, Si-Qi Zheng, Jia-Lin Zhao, Jun-Liang Zhang, Ying Huang, Zhang-Li Hu, Bin Jia
Summary: This study successfully introduced a novel isopentenol utilization pathway into Chlamydomonas reinhardtii, leading to a significant increase in limonene production. With culture optimization, transgenic algae cultured under a light: dark regimen achieved the highest limonene production of 117μg/L.
Article
Plant Sciences
Federico Perozeni, Matteo Pivato, Margherita Angelini, Elisa Maricchiolo, Andrea Pompa, Matteo Ballottari
Summary: This study presents a metabolic engineering strategy to produce high-value proteins in the green alga Chlamydomonas reinhardtii for nutritional purposes. By introducing a synthetic gene encoding a chimeric protein, zeolin, into the algal genome, researchers successfully achieved efficient expression and accumulation of zeolin protein in the endoplasmic reticulum of the alga. This development enables the production of microalga-based super-food.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Parminder Kaur Narang, Jyotirmayee Dey, Soumya Ranjan Mahapatra, Mrinmoy Ghosh, Namrata Misra, Mrutyunjay Suar, Vijay Kumar, Vishakha Raina
Summary: In this study, bioinformatics analysis was used to predict the function of a hypothetical protein from the model microalga Chlamydomonas reinhardtii, revealing its potential role in the carotenoid biosynthetic pathway. Homology modelling and docking analysis assisted in determining the protein's three-dimensional structure and active site region.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Biotechnology & Applied Microbiology
Alexander Einhaus, Jasmin Steube, Robert Ansgar Freudenberg, Jonas Barczyk, Thomas Baier, Olaf Kruse
Summary: In this study, innovative synthetic biology and efficient metabolic engineering strategies were combined to redirect metabolic flux through the MEP pathway in the green microalgae, C. reinhardtii, for efficient heterologous diterpenoid synthesis. Engineering of key enzymes and overexpression of fusion proteins successfully increased the production of high-value diterpenoids. Through fully photoautotrophic high cell density cultivations, significant amounts of sclareol, a high-value diterpenoid, were produced, demonstrating the potential of green microalgae as a powerful phototrophic cell factory for industrial biotechnology.
METABOLIC ENGINEERING
(2022)
Article
Environmental Sciences
Sanjukta Banerjee, Ayusmita Ray, Debabrata Das
Summary: Algal biomass is considered as a sustainable energy feedstock for future biofuel production. By optimizing physicochemical parameters, the biomass and carbohydrate productivity of Chlamydomonas reinhardtii were improved, leading to efficient bioethanol production and subsequent methane generation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Microbiology
Edoardo Andrea Cutolo, Giulia Mandala, Luca Dall'Osto, Roberto Bassi
Summary: Photosynthetic microbes are gaining attention as hosts for low-cost production of high-value recombinant proteins. Manipulation of unicellular algal genomes offers the opportunity to establish engineered strains as alternatives to conventional expression systems. Small-sized algal chloroplasts are excellent targets for synthetic biology approaches and compartmentalized accumulation of products. Expression of recombinant proteins, including therapeutic enzymes and peptides, has been successfully achieved in the chloroplasts of model organism Chlamydomonas reinhardtii and other species, demonstrating the potential of transplastomic algal biotechnology.
Article
Chemistry, Analytical
Maria Teresa Giardi, Daniele Zappi, Mehmet Turemis, Gabriele Varani, Fabrizio Lo Celso, Giampaolo Barone, Pietro Calandra, Amina Antonacci, Viviana Scognamiglio
Summary: This passage describes a method to design and synthesize novel artificial peptides that mimic the plastoquinone binding niche of the D1 protein from Chlamydomonas reinhardtii, with the ability to bind herbicides. These peptides were then functionalized with quantum dots for the development of a hybrid optosensor for the detection of atrazine, meeting E.U. legislation requirements with detection limits in the mu g/L concentration range.
Article
Biology
Debasmita Mondal, Ameya G. Prabhune, Sriram Ramaswamy, Prerna Sharma
Summary: Confining microorganisms, such as the microalga Chlamydomonas, between rigid boundaries leads to inversion of surrounding vortex flows, enhancing fluid mixing despite higher friction. Experimental insights and a simplified theoretical description based on a quasi-2D Brinkman approximation provide a comprehensive framework for analyzing the collective flows of strongly confined swimmers.
Article
Engineering, Chemical
Alysson Wagner Fernandes Duarte, Andre Moreni Lopes, Joao Vitor Dutra Molino, Adalberto Pessoa, Lara Duraes Sette
SEPARATION AND PURIFICATION TECHNOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Joao H. P. M. Santos, Iris M. Costa, Joao V. D. Molino, Mariana S. M. Leite, Marcela V. Pimenta, Joao A. P. Coutinho, Adalberto Pessoa, Sonia P. M. Ventura, Andre M. Lopes, Gisele Monteiro
BIOTECHNOLOGY PROGRESS
(2017)
Review
Biotechnology & Applied Microbiology
Joao Vitor Dutra Molino, Daniela de Araujo Viana Marques, Adalberto Pessoa Junior, Priscila Gava Mazzola, Maria Silvia Viccari Gatti
BIOTECHNOLOGY PROGRESS
(2013)
Article
Chemistry, Medicinal
Lorena Blau, Renato Farina Menegon, Gustavo H. G. Trossini, Joao Vitor Dutra Molino, Drielli Gomes Vital, Regina Maria Barretto Cicarelli, Gabriela Duo Passerini, Priscila Longhin Bosquesi, Chung Man Chin
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2013)
Meeting Abstract
Biochemical Research Methods
Alysson Duarte, Andre Lopes, Joao Molino, Adalberto Pessoa, Lara Sette
Meeting Abstract
Biochemical Research Methods
Adalberto Pessoa-, Gisele Monteiro, Joao Santos, Johanna Oses, Albert Peixoto, Juan Santos, Joao Molino, Laura Oliveira, Joao Coutinho, Sonia Ventura, Andre Lopes
Article
Engineering, Chemical
Renata Garcia Rodrigues Teixeira-Pinto, Joao Vitor Dutra Molino, Valeria Carvalho Santos-Ebinuma, Adalberto Pessoa, Sandro Roberto Valentini, Jorge Fernando Brandao Pereira, Andre Moreni Lopes
SEPARATION AND PURIFICATION TECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Cesar Andres Diaz Arias, Joao Vitor Dutra Molino, Daniela de Araujo Viana Marques, Andrea Queiroz Maranhao, Dulcineia Abdalla Saes Parra, Adalberto Pessoa Junior, Attilio Converti
MOLECULAR BIOLOGY REPORTS
(2019)
Article
Biotechnology & Applied Microbiology
Cesar Andres Diaz Arias, Marcelo Chuei Matsudo, Livia Seno Ferreira-Camargo, Joao Vitor Dutra Molino, Stephen Patrick Mayfield, Joao Carlos Monteiro de Carvalho
Summary: Advancements in biotechnology have made microalgae a promising platform for recombinant protein production. By utilizing a semicontinuous process with 60% culture withdrawal, the production of mCherry fluorescent protein showed a significant increase in productivity.
BIOTECHNOLOGY PROGRESS
(2021)
Article
Food Science & Technology
Talita C. E. S. Nascimento, Joao Vitor Dutra Molino, Priscila R. S. Donado, Gualberto S. A. Montalvo, Wellington L. dos Santos, Jose Erick G. Gomes, Joao H. P. M. Santos, Roberto da Silva, Lara Duraes Sette, Adalberto Pessoa Junior, Keila Aparecida Moreira
Summary: The extracellular serine protease produced by Acremonium sp. L1-4B isolated from the Antarctic continent was used to hydrolyze bovine and caprine sodium caseinate, resulting in protein hydrolysates with antioxidant and antihypertensive potential. Mass spectrometry characterization revealed differences in peptide sequences between the bovine and caprine hydrolysates, indicating the potential for developing new functional foods and nutraceutical formulations.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Engineering, Biomedical
Inga S. Shchelik, Joao V. D. Molino, Karl Gademann
Summary: Biohybrid microswimmers designed to carry antibiotics against bacterial infections utilize motile microorganisms and a photo-cleavable linker to release drugs on demand, providing high spatial and temporal precision in inhibiting bacterial growth.
ACTA BIOMATERIALIA
(2021)
Article
Biotechnology & Applied Microbiology
Joao Vitor Dutra Molino, Roberta Carpine, Karl Gademann, Stephen Mayfield, Simon Sieber
Summary: Cell-surface display systems are biotechnological techniques used to express heterologous proteins on the cell surface, and the choice of anchoring points depends on the cell system used. In this study, different anchoring points in the green algae Chlamydomonas were screened for efficiency and functionality, leading to the identification of two functional anchors. The use of GP1 protein in cell wall shedding provides a promising strategy for efficient protein release, supporting both cell biology studies and biotechnology applications.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Review
Nutrition & Dietetics
Crisandra J. Diaz, Kai J. Douglas, Kalisa Kang, Ashlynn L. Kolarik, Rodeon Malinovski, Yasin Torres-Tiji, Joao V. Molino, Amr Badary, Stephen P. Mayfield
Summary: Current agricultural and food production practices are under pressure to feed the growing human population while minimizing environmental impact. Algae, both macro and micro forms, have been cultivated for thousands of years and are highly efficient in resource utilization. They have the potential to address current food production issues due to their nutritious biomass and sustainable production methods.
FRONTIERS IN NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Joao Vitor Dutra Molino, Andre Moreni Lopes, Daniela de Araujo Viana Marques, Priscila Gava Mazzola, Joas Lucas da Silva, Mario Hiroyuki Hirata, Rosario Dominguez Crespo Hirata, Maria Silvia Viccari Gatti, Adalberto Pessoa
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2018)