Article
Biochemistry & Molecular Biology
Wenjun Guo, Mengmeng Wang, Lei Chen
Summary: The BacMam system, utilizing baculovirus as a delivery tool, is widely used for recombinant production of eukaryotic proteins in mammalian cells. In this study, a BacMam vector (pBMCL1) was developed, which allows easy tracking of virus production, enhances infection efficiency in mammalian cells, reduces unwanted transcription of toxic genes in insect cells, and enables the co-expression of multiple proteins using a single virus. The successful application of the pBMCL1 vector in expressing homo-tetrameric human TRPC3 channel and hetero-octameric KATP channel is demonstrated.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Pharmacology & Pharmacy
Hanan Bloomer, Jennifer Khirallah, Yamin Li, Qiaobing Xu
Summary: The CRISPR/Cas system has revolutionized the ability to edit the mammalian genome, providing a platform for correcting pathogenic mutations and studying gene function. Cas9 RNPs, as a delivery method, offer advantages in editing efficiency, off-target editing activity, and cellular toxicity reduction.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Multidisciplinary Sciences
Hui-Chung Lin, Der-Jiang Chiao, Chang-Chi Lin, Szu-Cheng Kuo
Summary: The study demonstrates efficient delivery of CHIKV replicons into cells using a baculovirus, verifying its efficacy through experiments, offering insights into virus replication mechanisms and potential for antiviral development.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
M. Maschietto, M. Dal Maschio, S. Girardi, S. Vassanelli
Summary: Electroporation is a widely utilized technique for non-viral delivery of molecules into cells, and recent advancements have miniaturized the machinery for in-situ genetic manipulation with high spatial selectivity and cell viability, even with differentiated neurons. SiO2 thin film-insulated microelectrodes support reliable and spatially selective electroporation and enable double and serial transfection of targeted cells.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Sabrina Amalfi, Maria del Pilar Plastine, Maria Gabriela Lopez, Maria Jose Gravisaco, Oscar Taboga, Victoria Alfonso
Summary: In this study, researchers demonstrated that the poxin homologous P26 from AcMNPV can degrade 2'3'-cGAMP, thus inhibiting the antiviral response and improving gene delivery efficiency. Incorporating P26 into baculoviral vectors significantly increased the efficiency of transgene expression.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Ronghui Zhu, Jesus M. del Rio-Salgado, Jordi Garcia-Ojalvo, Michael B. Elowitz
Summary: This study explores synthetic circuits that generate multiple stable states, providing insights into natural cell fate control circuit structures and enabling engineering of multicellular programs requiring interactions among distinct cell types.
Article
Virology
Sandra R. Abbo, Wilson Nguyen, Marleen H. C. Abma-Henkens, Denise van de Kamer, Niek H. A. Savelkoul, Corinne Geertsema, Thuy T. T. Le, Bing Tang, Kexin Yan, Troy Dumenil, Monique M. van Oers, Andreas Suhrbier, Gorben P. Pijlman
Summary: Mayaro virus is associated with acute rheumatic disease and has the potential to emerge as a tropical public health threat. Researchers developed a virus-like particle vaccine against Mayaro virus, which induced neutralizing antibodies and protected mice against infection and disease.
JOURNAL OF VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Roberto Di Blasi, Mara Pisani, Fabiana Tedeschi, Masue M. Marbiah, Karen Polizzi, Simone Furini, Velia Siciliano, Francesca Ceroni
Summary: Resource competition can cause unintended coupling between co-expressed genetic constructs. In this study, the authors quantified the resource load of different mammalian genetic components, identifying construct designs with increased performance and reduced resource footprint. By generating improved synthetic circuits and optimizing the co-expression of transfected cassettes, this work sheds light on their usefulness in bioproduction and biotherapeutic applications.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yifei Wang, Chun-Chi Chang, Fan Yuan
Summary: Electrotransfection is a non-viral method to deliver nucleic acids into cells. This study determined the minimal copy number of plasmid DNA (pDNA) per cell required for transgene expression and developed a statistical framework to predict pDNA copy number in the nucleus. A novel approach was developed to obtain isolated nuclei with minimal contamination by extranuclear pDNA, enabling accurate measurement of intranuclear pDNA. The data showed that the copy number of pDNA per nucleus depended on cell culture period and pDNA dose for electrotransfection, and were used to improve the understanding of pDNA transport kinetics in cells.
BIOELECTROCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Yue Wu, Afu Fu, Gilad Yossifon
Summary: In this study, a metallodielectric hybrid micromotor was used to perform localized electroporation and gene transfection of mammalian cells, showing high reversible electroporation yield and precise targeting of cells. The micromotor was able to enhance gene introduction into difficult-to-transfect cells and demonstrated successful gene expression post transfection, which is significant for molecular biology research.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Chemical
Chin-Wei Chang, Liang-Shin Wang, Nam Ngoc Pham, Chih-Che Shen, Mu-Nung Hsu, Nuong Thi Kieu Nguyen, Chia-Yi Yen, Mei-Wei Lin, Jih-Ru Hwu, Yu-Han Chang, Yu-Chen Hu
Summary: Researchers have developed a novel baculovirus vector that efficiently delivers genes into mammalian cells and prolongs transgene expression. This all-in-one vector helps to prevent gene loss during virus production and improves gene delivery efficiency, making it significant for gene therapy applications.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Multidisciplinary Sciences
Doudou Tan, Wei Wei, Zhen Han, Xuelian Ren, Cong Yan, Shankang Qi, Xiaohan Song, Y. George Zheng, Jiemin Wong, He Huang
Summary: This study identifies HBO1 as the writer of lysine benzoylation and reports 1747 Kbz sites, with at least 77 of them being targeted by HBO1. HBO1-targeted Kbz sites are involved in multiple biological processes.
Article
Biochemistry & Molecular Biology
Shibo Li, Hailong Wang, Sanaa Jehi, Jun Li, Shuo Liu, Zi Wang, Lan Truong, Takuya Chiba, Zefeng Wang, Xiaohua Wu
Summary: Break-induced replication (BIR) is a specialized pathway for DNA double-strand break (DSB) repair, with human PIF1 helicase playing an important role in promoting BIR in mammalian cells. AT-rich DNA sequences derived from fragile sites can induce BIR under replication stress and oncogenic stress. Loss of PIF1 is synthetically lethal with loss of FANCM, and a breast cancer-associated PIF1 mutant is defective in BIR, suggesting a direct link between BIR and oncogenic processes.
Article
Biochemistry & Molecular Biology
Liangli Wang, Chun-Chi Chang, Justin Sylvers, Fan Yuan
Summary: Plasmid DNA is commonly used for non-viral gene delivery, but only a fraction of molecules function as transcription templates in the nucleus. Researchers developed a statistical framework to establish the minimal amount of pDNA molecules needed for transgene expression. Experimental data under different conditions could be quantitatively explained or predicted using this framework.
BIOELECTROCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Alireza Milani, Azam Bolhassani, Fatemeh Rouhollah, Maryam Naseroleslami
Summary: The study evaluated the effects of heat shock on enhancing the transfection efficiency of plasmid DNAs into various cell lines, demonstrating the potential of thermal stimulation in improving gene transfection efficiency. Additionally, the research revealed a significantly lower DNA transfection efficiency in cancerous cells compared to non-cancerous cells.
BIOTECHNOLOGY LETTERS
(2021)
Review
Biotechnology & Applied Microbiology
Maysam Mansouri, Philipp Berger
BIOTECHNOLOGY ADVANCES
(2018)
Article
Multidisciplinary Sciences
Raphael S. Haider, Florian Wilhelm, Aurelien Rizk, Eshita Mutt, Xavier Deupi, Christian Peterhans, Jonas Muhle, Philipp Berger, Gebhard F. X. Schertler, Jorg Standfuss, Martin K. Ostermaier
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Ye Xie, Maysam Mansouri, Aurelien Rizk, Philipp Berger
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Martin Spillmann, Larissa Thurner, Nina Romantini, Mirjam Zimmermann, Benoit Meger, Martin Behe, Maria Waldhoer, Gebhard F. X. Schertler, Philipp Berger
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemical Research Methods
Stefanie Dobitz, Patrick Wilhelm, Nina Romantini, Martina De Foresta, Cornelia Walther, Andreas Ritler, Roger Schibli, Philipp Berger, Xavier Deupi, Martin Behe, Helma Wennemers
BIOCONJUGATE CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Anja Nitzsche, Riikka Pietila, Dominic T. Love, Chiara Testini, Takeshi Ninchoji, Ross O. Smith, Elisabet Ekvarn, Jimmy Larsson, Francis P. Roche, Isabel Egana, Suvi Jauhiainen, Philipp Berger, Lena Claesson-Welsh, Mats Hellstrom
Summary: Cell signalling is tightly regulated by specialized membranes and vesicles containing unique lipids and proteins. Paladin, identified as a PI(4,5)P-2 phosphatase, regulates VEGFR2 endosomal signalling and angiogenesis by interacting with VEGFR2 in endosomal and Golgi compartments. Inhibition of Paladin could potentially modulate VEGFR2 signalling and angiogenesis without directly inhibiting the receptor.
Article
Cell Biology
Maria Giovanna De Leo, Philipp Berger, Andreas Mayer
Summary: Autophagosome formation requires specific proteins and phosphoinositides, with WIPI1 playing a crucial role in endosomal trafficking. WIPI1 is involved in the formation and fission of tubulo-vesicular transport carriers, supporting the transport of cargo proteins towards various organelles. The inactivation of WIPI1 leads to a significant enlargement of endosomes and accumulation of long tubules, impacting both autophagy and protein exit from endosomes.
Article
Multidisciplinary Sciences
Nina Romantini, Shahidul Alam, Stefanie Dobitz, Martin Spillmann, Martina De Foresta, Roger Schibli, Gebhard F. X. Schertler, Helma Wennemers, Xavier Deupi, Martin Behe, Philipp Berger
Summary: This study demonstrates that using rigid oligoproline backbones can develop ligands with biased GPCR signaling, and stabilizing GPCR dimers by fixing the distance between their recognition motifs can expand the space for medicinal chemistry.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Nanoscience & Nanotechnology
Thomas Mortelmans, Dimitrios Kazazis, Celestino Padeste, Philipp Berger, Xiaodan Li, Yasin Ekinci
Summary: The development of a three-dimensional nanofluidic device for rapid and multiplexed detection of viral antibodies is presented. The device is made from poly(methyl methacrylate) and utilizes nanoscale topography variations for particle immobilization and trapping. It can be used for specific and sensitive multiplexed detection of serological antibodies against different viral proteins. The device's applications can be extended to detect various diseases simultaneously.
ACS APPLIED NANO MATERIALS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yun Qin, Stefan Imobersteg, Stephan Frank, Alain Blanc, Tanja Chiorazzo, Philipp Berger, Roger Schibli, Martin P. Behe, Michal Grzmil
Summary: α-particle emitters have potential as therapeutic radionuclides, but toxicity and radioresistance limit their efficacy. This study identified the radiation-activated mechanisms driving cancer cell survival, providing opportunities for therapeutic interference to improve the efficacy and safety of α-particle therapy.
JOURNAL OF NUCLEAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Rolf Antonie Loch, Hongzhi Wang, Alex Peralvarez-Marin, Philipp Berger, Henrietta Nielsen, Angeliki Chroni, Jinghui Luo
Summary: This article summarizes the interactions between different isoforms of Apolipoprotein E (ApoE) and amyloid proteins, highlighting their isoform-specific roles in amyloid-based neurodegenerative diseases. Although the molecular and genetic interactions of ApoE with amyloid proteins have been extensively studied, there is still a need to connect and clarify them. This research is crucial for understanding neurodegenerative diseases.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Engineering, Electrical & Electronic
Maryam Moazeni, Philipp Berger, Celestino Padeste
Summary: Development of rapid, point-of-care (POC) diagnostic tools using hairpin-like DNA or molecular beacon probes has significant potential for disease surveillance and monitoring, particularly in underdeveloped or developing countries. This research focuses on 3D polymer brush structures with antifouling properties, functionalized with specific DNA probes, for fluorescence-based DNA detection at micromolar concentrations. The polymer brush structures provide hydrophilicity and epoxide functional groups for further functionalization, and embedding them into a microfluidic channel coupled with a mobile-phone-based fluorescence microscope allows for signal detection.
MICRO AND NANO ENGINEERING
(2023)
Article
Medicine, Research & Experimental
Michal Grzmil, Yun Qin, Carina Schleuniger, Stephan Frank, Stefan Imobersteg, Alain Blanc, Martin Spillmann, Philipp Berger, Roger Schibli, Martin Behe
Meeting Abstract
Biochemical Research Methods
Nina Romantini, Stefanie Dobitz, Shahidul Alam, Martin Spillmann, Roger Schibli, Xavier Deupi, Helma Wennemers, Philipp Berger, Martin Behe
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2019)
