Article
Plant Sciences
Xiahui Hao, Wenchao Chen, Alberto Amato, Juliette Jouhet, Eric Marechal, Daniel Moog, Hanhua Hu, Hu Jin, Lingjie You, Fenghong Huang, Mark Moosburner, Andrew E. Allen, Yangmin Gong
Summary: This study utilized multiplexed CRISPR/Cas9 to generate gene knockout mutants with large fragment deletion, revealing the functions of LACS isozymes ptACSL3 and ptACSL5 in the oleaginous microalgae Phaeodactylum tricornutum and their impact on lipid synthesis.
Article
Chemistry, Medicinal
Victor Murison, Josiane Herault, Benoit Schoefs, Justine Marchand, Lionel Ulmann
Summary: Through genomic analysis, researchers have made progress in understanding the lipid degradation mechanisms of oleaginous diatoms, identifying 57 potential enzymes involved. Further analysis revealed that 9 genes were highly regulated under nitrogen starvation conditions, suggesting an important role in lipid degradation. Tertiary structure prediction highlighted two downregulated enzymes as potential targets for future functional genomics and purification studies to investigate their role in lipid degradation.
Article
Plant Sciences
Yufang Pan, Wanting Zhang, Xiaofei Wang, Juliette Jouhet, Eric Marechal, Jin Liu, Xiao-Qin Xia, Hanhua Hu
Summary: This study explores the allele-specific expression of PDAT gene in the model diatom Phaeodactylum tricornutum. The results show that one of the alleles, Allele 2, can be specifically induced by decreasing nitrogen concentrations and overexpression of Allele 2 substantially enhances the accumulation of TAG. On the other hand, overexpression of Allele 1 only slightly increases TAG accumulation but leads to more serious growth inhibition. It is also found that the enzymes encoded by the two alleles have different TAG biosynthetic activity, which is mainly affected by amino acid variants in their protein sequences.
Article
Biotechnology & Applied Microbiology
Qin Shu, Yufang Pan, Hanhua Hu
Summary: In this study, a homolog of CGI-58 called PtCGI-58 was identified in Phaeodactylum tricornutum. PtCGI-58 was found to be localized in mitochondria, unlike its localization in animals and plants. Overexpression of PtCGI-58 resulted in increased neutral lipid content, TAG accumulation, and an almost doubled EPA content in TAGs. Furthermore, genes involved in fatty acid synthesis and mitochondrial beta-oxidation were significantly upregulated in PtCGI-58 overexpression strains. These findings suggest that PtCGI-58 may play a role in lipid breakdown in mitochondria and recycling of acyl chains into TAG biosynthesis, and provide insights into new functions of CGI-58 in lipid homeostasis.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Chen Chen, Xue Li, Huajun Zhao, Meng Liu, Jintong Du, Jian Zhang, Xinying Yang, Xuben Hou, Hao Fang
Summary: The combined use of a DNA minor groove binder and a histone deacetylase (HDAC) inhibitor has shown a synergistic antiproliferation effect. A new series of benzimidazole-hydroxamate hybrids were designed and synthesized to target both DNA minor groove and HDAC. The most active compounds 9k and 9l not only exhibited improved HDAC inhibitory activities but also showed potent antiproliferation activities against tumor cells. Importantly, these compounds demonstrated good in vivo antitumor efficacies and reshaped the tumor immune microenvironment by promoting antigen presentation, activating T cells, and polarizing tumor-infiltrating macrophages with antitumor activity.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Environmental Sciences
Leyla Uslu, Oya Isik, Mahamad Ahmat Hamid, Burcu Ak Cimen
Summary: The study aimed to determine the effect of different salinity concentrations on the biomass productivity and fucoxanthin contents of Phaeodactylum tricornutum, Thalassiosira weissflogii and Nitzschia closterium forma minutissima biomass. Results showed that N. closterium f. minutissima had the highest biomass and fucoxanthin content under 30 parts per thousand salinity, making it the most promising species for further research in this study.
FRESENIUS ENVIRONMENTAL BULLETIN
(2021)
Article
Plant Sciences
Sergio Balzano, Laura Villanueva, Marijke de Bar, Diana X. Sahonero Canavesi, Caglar Yildiz, Julia C. Engelmann, Eric Marechal, Josselin Lupette, Jaap S. Sinninghe Damste, Stefan Schouten
PLANT AND CELL PHYSIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Eric Marechal, Josselin Lupette
Article
Plant Sciences
Solene Breton, Juliette Jouhet, Ulysse Guyet, Valerie Gros, Justine Pittera, David Demory, Frederic Partensky, Hugo Dore, Morgane Ratin, Eric Marechal, Ngoc An Nguyen, Laurence Garczarek, Christophe Six
Article
Biochemistry & Molecular Biology
Muge Senarisoy, Caroline Barette, Francoise Lacroix, Salvatore De Bonis, Meike Stelter, Fabienne Hans, Jean-Philippe Kleman, Marie-Odile Fauvarque, Joanna Timmins
ACS CHEMICAL BIOLOGY
(2020)
Article
Plant Sciences
Antoine Jaussaud, Josselin Lupette, Juliette Salvaing, Juliette Jouhet, Olivier Bastien, Marina Gromova, Eric Marechal
FRONTIERS IN PLANT SCIENCE
(2020)
Review
Biochemistry & Molecular Biology
Josselin Lupette, Christoph Benning
Article
Pharmacology & Pharmacy
Sacnicte Ramirez-Rios, Sophie Michallet, Leticia Peris, Caroline Barette, Clotilde Rabat, Yangbo Feng, Marie-Odile Fauvarque, Annie Andrieux, Karin Sadoul, Laurence Lafanechere
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Biochemical Research Methods
Emmanuelle Soleilhac, Marjorie Comte, Anaelle da Costa, Caroline Barette, Christele Picoli, Magda Mortier, Laurence Aubry, Franck Mouthon, Marie-Odile Fauvarque, Mathieu Charveriat
Summary: This study developed automated cell-based assays to identify new pharmacological modulators of Cx43-HCs, leading to the discovery of 11 candidate hits that block Cx43-HC, with 5 drugs being active on HCs but not on gap junction activities. These findings represent the first screening on HC activity and suggest the potential of repurposing approved drugs for central nervous system disorders with HC impairments.
Article
Oncology
Sofia Giacosa, Catherine Pillet, Irinka Seraudie, Laurent Guyon, Yann Wallez, Caroline Roelants, Christophe Battail, Bertrand Evrard, Frederic Chalmel, Caroline Barette, Emmanuelle Soleilhac, Marie-Odile Fauvarque, Quentin Franquet, Clement Sarrazin, Nicolas Peilleron, Gaelle Fiard, Jean-Alexandre Long, Jean-Luc Descotes, Claude Cochet, Odile Filhol
Summary: Renal cell carcinoma (RCC) is a significant malignancy globally, with poor outcomes when metastatic. Protein kinases play a crucial role in cancer progression, and targeting CK2 and ATM kinases can induce synthetic lethality in RCC cells. The study highlights the potential of targeting CK2 and ATM kinases in VHL-deficient renal carcinoma cells to trigger ROS-dependent apoptosis and improve patient care in metastatic RCC.
Review
Cell Biology
Ron Cook, Josselin Lupette, Christoph Benning
Summary: Plants can adapt to environmental changes by remodeling their membrane lipid composition and releasing fatty acids in response to environmental cues. Chloroplasts, as the photosynthetic apparatus, have an extensive and complex membrane system.
Article
Biochemistry & Molecular Biology
Farah Kobaisi, Eric Sulpice, Caroline Barette, Nour Fayyad, Marie-Odile Fauvarque, Bassam Badran, Mohammad Fayyad-Kazan, Hussein Fayyad-Kazan, Xavier Gidrol, Walid Rachidi
Summary: XPC plays a critical role in DNA repair mechanisms and its mutations lead to increased photosensitivity and cancer risk among XP-C patients. A high-throughput screening identified two chemicals, Isoconazole and Clemizole Hydrochloride, that partially inhibit cell apoptosis and promote cell survival in XP-C fibroblasts. These compounds show potential in ameliorating XP-C phenotype but further research is needed to understand their molecular mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Rodrigo Enrique Gomez, Josselin Lupette, Clement Chambaud, Julie Castets, Amelie Ducloy, Jean-Luc Cacas, Celine Masclaux-Daubresse, Amelie Bernard
Summary: Plants rely on autophagy to respond to environmental and developmental stresses, mediating the degradation and recycling of cellular material. The formation of autophagosomes requires membrane remodeling events where the quantity and quality of lipids are crucial for sustaining autophagy activity.
Article
Biochemistry & Molecular Biology
Hamza Mameri, Geraldine Buhagiar-Labarchede, Gaelle Fontaine, Celine Corcelle, Caroline Barette, Rosine Onclercq-Delic, Claire Beauvineau, Florence Mahuteau-Betzer, Mounira Amor-Gueret
Summary: Identifying new molecular targets for novel anticancer treatments is a major challenge in clinical cancer research. In this study, the researchers aimed to develop a new anticancer treatment specifically inhibiting the growth of CDA-deficient tumor cells. They identified a naphthol derivative, X55, that selectively targeted CDA-deficient tumor cells without affecting non-tumoral cells. Metabolomic profiling revealed differences in the metabolome of CDA-deficient cells compared to control cells, and X55 treatment exacerbated these differences.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lou Saier, Johnny Ribeiro, Thomas Daunizeau, Audrey Houssin, Gabriel Ichim, Caroline Barette, Lamia Bouazza, Olivier Peyruchaud
Summary: Metastasis is a major cause of cancer-related deaths. Platelets play a role in cancer progression but are challenging to target due to their role in hemostasis. Anti-asthmatic drugs zafirlukast and montelukast have been identified as specific blockers of platelet protumoral action. These drugs inhibit platelet interaction with breast cancer cells and prevent cancer cell dissemination and metastasis without affecting primary tumor growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Agnes Journet, Caroline Barette, Laurence Aubry, Emmanuelle Soleilhac, Marie-Odile Fauvarque
Summary: The ubiquitin-specific protease USP8 plays a major role in controlling the stability and intracellular trafficking. This study investigates the interaction between USP8 and CHMP1B and identifies key components and regulatory mechanisms involved in this interaction through chemical inhibitor screening and variant experiments.