期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 133, 期 3, 页码 385-393出版社
SPRINGER
DOI: 10.1007/s11240-018-1391-z
关键词
Androgenesis; Anther culture; Haploid; Microspore culture; Spelt wheat
资金
- scientific projects (name of project: Improvement of spelt wheat lines with low fermentable carbohydrate content (FODMAP) using modern and classical research methods) [OTKA-K_16-K119835]
- GINOP project [GINOP-2.2.1-15-2016-00026]
- Foundation of Szegedert
- Hungarian Academy of Sciences
This is the first report on isolated microspore culture-derived spelt wheat. The efficiency of anther- and isolated microspore was compared using four genotypes ('Franckenkorn', 'GK Feh,r', 'Mv Martongold', 'Oberkulmer Rotkorn'). In anther culture, genotype dependency was observed, and cold pre-treatment enhanced the efficiency of the method. In isolated microspore culture, the ovary co-culture supported the development of embryo-like structures. The presence of growth regulators (0.5 mg/l 2,4-D and 0.5 mg/l kinetin) were not essential for the induction of androgenesis, but these increased the production of embryo-like structures, green and albino plantlets. The low plant regeneration rate and high number of albinos hinder the practical application of isolated microspore culture while anther culture was efficient for in vitro green plantlets production in spelt wheat. The mean of green plantlets production was 41.45/100 anthers (from 20.93 to 83.07 depending on genotype). The phenomenon of albinism was mitigated in anther culture (3.48 albinos/100 anthers). Altogether, 1720 anther culture-derived green plantlets were produced from the four genotypes.
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