Article
Biochemistry & Molecular Biology
Liang Wang, Taru Hilander, Xiaonan Liu, Hoi Ying Tsang, Ove Eriksson, Christopher B. Jackson, Markku Varjosalo, Hongxia Zhao
Summary: This study identifies GTPBP8 as a crucial factor in the assembly of the mitoribosomal large subunit (mt-LSU) and mitochondrial translation. Depletion of GTPBP8 leads to abnormal accumulation of mt-LSU and compromised mitochondrial function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Oncology
Bartolomeo Bosco, Annalisa Rossi, Dario Rizzotto, Meriem Hadjer Hamadou, Alessandra Bisio, Sebastiano Giorgetta, Alicia Perzolli, Francesco Bonollo, Angeline Gaucherot, Frederic Catez, Jean-Jacques Diaz, Erik Dassi, Alberto Inga
Summary: Translation machinery in cells is coordinated by DHX30, affecting cell proliferation and energy metabolism. Silencing DHX30 enhances cytoplasmic ribosomal proteins translation while reducing mitoribosomal proteins translation, potentially impacting cancer cell fitness. Targeting DHX30 could exploit a vulnerability in cancer cells.
Article
Biochemistry & Molecular Biology
Franziska Nadler, Elena Lavdovskaia, Ricarda Richter-Dennerlein
Summary: The translation process in mitochondria, especially focusing on termination, recycling, and rescue mechanisms of mitoribosomes, is essential for maintaining cellular homoeostasis. Mitochondrial ribosomes are highly specialized in synthesizing core subunits of oxidative phosphorylation complexes, originated from bacterial ancestors but with substantial differences due to their endosymbiotic environment.
Review
Cell Biology
Taru Hilander, Christopher B. Jackson, Marius Robciuc, Tanzeela Bashir, Hongxia Zhao
Summary: Mitochondria, ancient bacterial endosymbionts of eukaryotic cells, have their own circular DNA and protein translation system. Recent advancements in cryoelectron microscopy and mass spectrometry have revealed the evolutionary divergence of mitochondrial ribosomes from their bacterial ancestors and their adaptation for synthesizing mitochondrial DNA encoded oxidative phosphorylation complex subunits. Furthermore, assembly pathway maps of mitoribosomes and associated factors have started to emerge, shedding light on the complex assembly process.
Review
Cell Biology
Fei Wang, Deyu Zhang, Dejiu Zhang, Peifeng Li, Yanyan Gao
Summary: Mitochondria in cells are crucial organelles with their own genetic system, and their protein translation process is regulated by various factors that, when abnormal, can lead to diseases. The balance between mitochondrial protein translation and cytoplasmic translation is also important for physiological and pathological processes in the body.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Antonios Apostolopoulos, Shintaro Iwasaki
Summary: Apart from the translation system in the cytoplasm, eukaryotic cells also have additional protein synthesis machinery in mitochondria. Mutations in mitochondrial translation factors are associated with clinical pathologies, highlighting the importance of in organello translation. The recent development of new approaches has allowed for quantitative, comprehensive, and spatiotemporal measurements of mitochondrial protein synthesis, leading to a better understanding of this unique translation system.
JOURNAL OF BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Franziska Nadler, Ricarda Richter-Dennerlein
Summary: Mitochondria play a crucial role in ATP production through oxidative phosphorylation in eukaryotes, relying on the maintenance and accurate expression of the mitochondrial genome. While translation principles are conserved, there are deviations in translation factors, mRNA characteristics, and the genetic code in human mitochondria. This poses challenges for mitochondrial translation. This article discusses the current understanding of mitochondrial translation, focusing on the termination process and quality control mechanisms, and highlights the importance of ribosome-associated quality control for respiratory function and human health.
BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Sylvain Delaunay, Gloria Pascual, Bohai Feng, Kevin Klann, Mikaela Behm, Agnes Hotz-Wagenblatt, Karsten Richter, Karim Zaoui, Esther Herpel, Christian Munch, Sabine Dietmann, Jochen Hess, Salvador Aznar Benitah, Michaela Frye
Summary: The study reveals that the NSUN3-dependent RNA modifications of mitochondrial mRNA can impact metabolic plasticity, thus affecting the metastatic potential of cancer cells, providing new therapeutic targets for combating cancer metastasis.
Review
Biochemistry & Molecular Biology
Annalisa Pecoraro, Martina Pagano, Giulia Russo, Annapina Russo
Summary: Cytosolic and mitochondrial ribosomes play crucial roles in protein synthesis and cellular functions. The biogenesis of ribosomes is tightly regulated and sensitive to various cellular stressors. Alterations in ribosome biogenesis are associated with tumorigenesis, and understanding the extra-ribosomal functions of ribosomal proteins is important for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Xiaowen Ma, Sharon Manley, Hui Qian, Yuan Li, Chen Zhang, Kevin Li, Benjamin Ding, Fengli Guo, Allen Chen, Xing Zhang, Meilian Liu, Meihua Hao, Benjamin Kugler, E. Matthew Morris, John Thyfault, Ling Yang, Hiromi Sesaki, Hong-Min Ni, Heidi McBride, Wen-Xing Ding
Summary: During hepatocyte dedifferentiation, a newly discovered intracellular organelle called the mitochondria-lysosome-related organelle (MLRO) plays a crucial role in regulating mitochondrial homeostasis. The MLRO is formed through the fusion of mitochondria-derived vesicles (MDVs) with lysosomes and is involved in mitochondrial protein degradation and hepatocyte dedifferentiation. This organelle, resembling damaged lysosomes, can be cleared by overexpression of the transcription factor EB (TFEB). The MLRO acts as an alternative mechanism for mitochondrial quality control during cell dedifferentiation, independent of canonical autophagy/mitophagy.
Article
Biochemistry & Molecular Biology
Inayathulla Mohammed, Kai A. Schmitz, Niko Schenck, Dimitrios Balasopoulos, Annika Topitsch, Timm Maier, Jan Pieter Abrahams
Summary: The mitochondrial Lon protease, LonP1, plays a crucial role in maintaining mitochondrial health by removing redundant proteins from the mitochondrial matrix. Cryo-EM analysis revealed eight nucleotide-dependent conformational states of LonP1. The study also showed how sequential ATP hydrolysis controls the translocation of substrate proteins in a 6-fold binding change mechanism.
Review
Biochemistry & Molecular Biology
Nhu Dinh, Nathalie Bonnefoy
Summary: Schizosaccharomyces pombe (fission yeast) is a valuable model for studying mitochondrial processes. It shares similarities with human cells in terms of mitochondrial inheritance, transport, metabolism, and dependence on the mitogenome for viability. The gene expression machinery is conserved between fission yeast and humans. With extensive experimental techniques and database resources, fission yeast has made significant contributions to biomedical and fundamental research, particularly in the field of mitochondrial translation.
Article
Biology
Yaqin Yan, Jintian Tang, Qinfeng Yuan, Caiyun Liu, Xiaolin Chen, Hao Liu, Junbin Huang, Chonglai Bao, Tom Hsiang, Lu Zheng
Summary: Prohibitins play important roles in fungal virulence by regulating mitophagy, affecting the pathogenicity of Colletotrichum higginsianum.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Yuzuru Itoh, Vivek Singh, Anas Khawaja, Andreas Naschberger, Minh Duc Nguyen, Joanna Rorbach, Alexey Amunts
Summary: The mitoribosome, an important component regulating cellular energy production, is associated with aging. This study reveals the roles of non-protein components in the mitoribosome through analyzing the structure of the small subunit in complex with the anti-tuberculosis drug streptomycin. Findings of iron-sulfur clusters and NAD as fundamental building blocks directly link to mitochondrial disease and aging.
Article
Plant Sciences
Chuande Wang, Lisa Blondel, Martine Quadrado, Celine Dargel-Graffin, Hakim Mireau
Summary: Gene expression in plant mitochondria is mainly regulated at the post-transcriptional level, and the MTSF3 protein plays an important role in stabilizing the nad2 transcript and maintaining mitochondrial function. It is essential for embryonic development in Arabidopsis thaliana.
Article
Biochemistry & Molecular Biology
Janusz Piechota, Monika Bereza, Aleksandra Sokolowska, Kondrad Suszynski, Karolina Lech, Hanna Janska
PLANT MOLECULAR BIOLOGY
(2015)
Article
Plant Sciences
Elwira Smakowska, Renata Skibior-Blaszczyk, Malgorzata Czarna, Marta Kolodziejczak, Malgorzata Kwasniak-Owczarek, Katarzyna Parys, Christiane Funk, Hanna Janska
Article
Biochemistry & Molecular Biology
Magdalena Opalinska, Katarzyna Parys, Hanna Janska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Cell Biology
Magdalena Opalinska, Katarzyna Parys, Monika W. Murcha, Hanna Janska
JOURNAL OF CELL SCIENCE
(2018)
Article
Plant Sciences
Iwona Migdal, Renata Skibior-Blaszczyk, Malgorzata Heidorn-Czarna, Marta Kolodziejczak, Arnold Garbiec, Hanna Janska
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Alicja Dolzblasz, Edyta M. Gola, Katarzyna Sokolowska, Elwira Smakowska-Luzan, Adriana Twardawska, Hanna Janska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Plant Sciences
Malgorzata Heidorn-Czarna, Dominik Domanski, Malgorzata Kwasniak-Owczarek, Hanna Janska
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Multidisciplinary Sciences
Alicja Dolzblasz, Elwira Smakowska, Edyta M. Gola, Katarzyna Sokolowska, Marta Kicia, Hanna Janska
SCIENTIFIC REPORTS
(2016)
Review
Cell Biology
Magdalena Opalinska, Hanna Janska
Article
Biochemistry & Molecular Biology
Malgorzata Kwasniak-Owczarek, Urszula Kazmierczak, Artur Tomal, Pawel Mackiewicz, Hanna Janska
NUCLEIC ACIDS RESEARCH
(2019)
Review
Cell Biology
Artur Tomal, Malgorzata Kwasniak-Owczarek, Hanna Janska
Article
Biology
Aleksandra Adamowicz-Skrzypkowska, Malgorzata Kwasniak-Owczarek, Olivier Van Aken, Urszula Kazmierczak, Hanna Janska
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Article
Plant Sciences
Agata Maziak, Malgorzata Heidorn-Czarna, Aleksandra Weremczuk, Hanna Janska
Summary: Research shows that in long-term moderate heat stress, Arabidopsis plants lacking mitochondrial proteases FTSH4 or OMA1 exhibit phenotypic changes associated with accumulation of small heat-shock proteins. The proteolytic activity of FTSH4 and OMA1 plays a crucial role in protecting mitochondrial proteins against aggregation under stress. Overall, the data suggest that FTSH4 and OMA1 increase plant tolerance to long-term moderate heat stress by reducing detergent-tolerant mitochondrial protein aggregation.
Review
Biochemistry & Molecular Biology
Malgorzata Czarna, Marta Kolodziejczak, Hanna Janska