Review
Biochemistry & Molecular Biology
Jun Yang, Liying Lan, Yue Jin, Nan Yu, Dong Wang, Ertao Wang
Summary: This article summarizes the recent advances in understanding the signaling, transcriptional regulation, and regulation of plant immunity during the legume-rhizobium symbiosis. It also proposes important questions to be addressed and provides insights into the potential for engineering nitrogen-fixing capacity in legume and non-legume plants.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Review
Plant Sciences
Jawahar Singh, Mariel C. Isidra-Arellano, Oswaldo Valdes-Lopez
Summary: Many plants form associations with AM fungi and rhizobia for nutrient acquisition, but these associations are regulated by the perception of LCOs. Cereals can perceive LCOs better in Pi and nitrogen-deficient soil, leading to efficient AM symbiosis. However, Pi deficiency hinders legumes' association with rhizobia, reducing nitrogen fixation.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Sanhita Chakraborty, Oswaldo Valdes-Lopez, Christina Stonoha-Arther, Jean-Michel Ane
Summary: This article explores the transcriptional regulation mechanism of the symbiotic relationship between legume roots and rhizobia, highlighting the role of certain transcription factors in symbiosis and the response to the abiotic environment. The article also discusses the potential utility of modern methods in studying transcription factor-target interactions in plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chunmei He, Hui Gao, Haijiao Wang, Yun Guo, Miao He, Yaqi Peng, Xuelu Wang
Summary: The study reveals that GmSK2-8 inhibits symbiotic signaling and nodule formation in soybean under salt stress by phosphorylating the LHRI domain of GmNSP1a, reducing plant sensitivity to salt stress during nodule formation. This provides novel targets for improving symbiotic nitrogen fixation under environmental stress conditions in soybean and possibly other legumes.
Review
Plant Sciences
Mina Ghahremani, Allyson M. MacLean
Summary: Post-embryonic organogenesis in plants enables them to be developmentally responsive to their environment, leading to opportunities for growth and structural remodeling that are unique compared to other higher order organisms. The plasticity in plant development makes plant-microbe interactions a fascinating field to study symbiosis. This review focuses on how beneficial microbes alter root growth, development, and architecture, dissecting the cell biology and molecular mechanisms underlying these interactions in a tissue-specific manner.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Forestry
Yu-E Ding, Ying-Ning Zou, Qiang-Sheng Wu, Kamil Kuca
Summary: The present study found that arbuscular mycorrhizal fungi can regulate the circadian clock in trifoliate orange plants under drought conditions. AMF inoculation significantly improved plant growth, photosynthetic rate and stomatal conductance, and increased ABA levels and antioxidant enzyme gene expression in the roots.
Review
Plant Sciences
Michael Paries, Caroline Gutjahr
Summary: Phosphate is vital for life, but it is poorly accessible for sessile land plants. To overcome this limitation, plants have developed various strategies for enhanced acquisition and recycling of phosphate. These strategies involve a conserved phosphate starvation response (PSR) system, as well as interactions with mycorrhiza fungi and other microbes. The PSR pathway not only regulates phosphate uptake but also influences plant immunity and can be manipulated by microbes.
Article
Biochemistry & Molecular Biology
Sonali Roy, Andrew Breakspear, Donna Cousins, Ivone Torres-Jerez, Kirsty Jackson, Anil Kumar, Yangyang Su, Cheng-Wu Liu, Nick Krom, Michael Udvardi, Ping Xu, Jeremy D. Murray
Summary: Three previously unreported ABC subfamily B transporters, named AMN1, AMN2, and AMN3, are strongly expressed in symbiotically infected tissues, with critical roles in nodulation and mycorrhization. Further studies are needed to identify potential substrates of these transporters and understand their roles in beneficial symbioses.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Akihiro Yamazaki, Kai Battenberg, Yoshikazu Shimoda, Makoto Hayashi
Summary: LysM-like receptor kinases play important roles in both plant immunity and symbiosis, particularly in signal perception and activation of downstream signal cascades. The study revealed that NHL13 interacts with NFR1 and SymRK, suggesting its involvement in both plant immunity and symbiosis.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Plant Sciences
Eva Nouri, Rohini Surve, Laure Bapaume, Michael Stumpe, Min Chen, Yunmeng Zhang, Carolien Ruyter-Spira, Harro Bouwmeester, Gaeetan Glauser, Sebastien Bruisson, Didier Reinhardt
Summary: This study explores the impact of phosphate (P-i) on the symbiotic relationship between plants and fungi, revealing that P-i may interfere with AM symbiosis via the phytohormone gibberellic acid (GA). Using Solanaceous model plants, it was found that GA-defective plants significantly affected the colonization of AM fungi, and exogenous phosphate had less inhibitory effect on these plants. The findings provide insights into potential targets for crop breeding to reduce P suppression of AM symbiosis and reconcile the benefits of P-i fertilization with AM symbiosis.
PLANT AND CELL PHYSIOLOGY
(2021)
Review
Plant Sciences
Divya Jain, Lauren Jones, Sonali Roy
Summary: Several gene editing techniques, including CRISPR-Cas9-based tools, have been developed for studying legume-rhizobia symbiosis. Gene editing has played a crucial role in identifying gene functions, validating genetic screens, resolving gene redundancy, and investigating symbiotic signaling networks in non-model plants. The integration of environmental signals with nitrogen fixation for climate-hardy legumes is important for their optimal functioning under varying conditions.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Article
Plant Sciences
Christine Seemann, Carolin Heck, Stefanie Voss, Jana Schmoll, Eileen Enderle, Diana Schwarz, Natalia Requena
Summary: Root development is a crucial process for plants to acquire nutrients, adapt to the substrate, and withstand changing environmental conditions. The plasticity of roots is controlled by a variety of transcriptional regulators, allowing post-embryonic changes that give rise to new tissue and specialized cells. One important change is the accommodation of arbuscular mycorrhizal fungi in the cortex, where cells undergo massive reprogramming to coordinate developmental changes with transport processes.
Article
Plant Sciences
Liangliang Yu, Qi Di, Danping Zhang, Yumin Liu, Xiaolin Li, Kirankumar S. Mysore, Jiangqi Wen, Junhui Yan, Li Luo
Summary: PSK-delta, a novel legume-specific phytosulfokine, promotes symbiotic nodulation by enhancing nodule organogenesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Laurent Sauviac, Antoine Remy, Emeline Huault, Melanie Dalmasso, Theophile Kazmierczak, Marie-Francoise Jardinaud, Ludovic Legrand, Corentin Moreau, Bryan Ruiz, Anne-Claire Cazale, Sophie Valiere, Benjamin Gourion, Laurence Dupont, Veronique Gruber, Eric Boncompagni, Eliane Meilhoc, Pierre Frendo, Florian Frugier, Claude Bruand
Summary: This study identified hundreds of plant and bacterial genes associated with nodule senescence through dual plant-bacteria RNA sequencing, providing a valuable resource for exploring the mechanisms of nodule senescence. The study revealed that genes related to both plant and bacterial cell cycle and stress responses play important roles in nodule senescence, suggesting complex regulatory networks involved in this process.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Yancui Jia, Youguo Li
Summary: This study identifies and analyzes the RALF gene family in Arabidopsis, soybean, Lotus, and Medicago. It reveals the evolutionary mechanism of RALFs in symbiosis and their potential involvement in nodule release.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chao Su, Marie-Luise Klein, Casandra Hernandez-Reyes, Morgane Batzenschlager, Franck Anicet Ditengou, Beatrice Lace, Jean Keller, Pierre-Marc Delaux, Thomas Ott
Article
Biochemistry & Molecular Biology
Pengbo Liang, Clara Schmitz, Beatrice Lace, Franck Anicet Ditengou, Chao Su, Eija Schulze, Julian Knerr, Robert Grosse, Jean Keller, Cyril Libourel, Pierre-Marc Delaux, Thomas Ott
Summary: Legumes have the ability to associate with rhizobia for nitrogen-fixing root nodule symbiosis. In Medicago truncatula, root hair colonization by Sinorhizobium meliloti begins from young root hairs, which physically entwine around the symbiont to form rhizobial traps. This unique process involves alterations in membrane organization, cytosolic calcium gradient, actin rearrangements, and symbiotic responses in legumes.
Article
Plant Sciences
Moira Giovannoni, Lucia Marti, Simone Ferrari, Natsuki Tanaka-Takada, Masayoshi Maeshima, Thomas Ott, Giulia De Lorenzo, Benedetta Mattei
Summary: This study demonstrates the essential role of the protein PCaP1 in mediating late responses to oligogalacturonides and flagellin. PCaP1 is not involved in early responses but may be important for maintaining its stability on the plasma membrane through endocytic turnover. The findings suggest a mechanism of PCaP1 endocytosis induced by oligogalacturonides.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Melanie K. Rich, Nicolas Vigneron, Cyril Libourel, Jean Keller, Li Xue, Mohsen Hajheidari, Guru Radhakrishnan, Aurelie Le Ru, Seydina Issa Diop, Giacomo Potente, Elena Conti, Danny Duijsings, Aurelie Batut, Pauline Le Faouder, Kyoichi Kodama, Junko Kyozuka, Erika Sallet, Guillaume Becard, Marta Rodriguez-Franco, Thomas Ott, Justine Bertrand-Michel, Giles E. D. Oldroyd, Peter Szovenyi, Marcel Bucher, Pierre-Marc Delaux
Summary: Symbiosis with arbuscular mycorrhizal fungi benefits plant nutrition and elicits a conserved transcriptomic response among land plants, including activation of lipid metabolism. Transfer of lipids from liverwort to AMF is regulated by the transcription factor WRINKLED, and the formation of arbuscules is essential for mutualism. The symbiotic transfer of lipids is orthologous across land plants, indicating the presence of mutualism with AMF in the most recent ancestor of land plants 450 million years ago.
Article
Biochemistry & Molecular Biology
Casandra Hernandez-Reyes, Elisabeth Lichtenberg, Jean Keller, Pierre-Marc Delaux, Thomas Ott, Sebastian T. Schenk
Summary: This study investigated the response of the non-legume plant Arabidopsis to changes in root system architecture induced by rhizobia under different nitrate conditions. The study found that rhizobium-induced lateral root growth, increased root hair length, and density are regulated by a nitrate-related signaling pathway. AtNLP4 and AtNLP5 were identified as key players in this process. At the cellular level, AtNLP4 and AtNLP5 control a rhizobium-induced decrease in cell elongation rates.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Chao Su, Guofeng Zhang, Marta Rodriguez-Franco, Rosula Hinnenberg, Jenny Wietschorke, Pengbo Liang, Wei Yang, Leonard Uhler, Xia Li, Thomas Ott
Summary: The root nodule symbiosis is a process in which legume roots are colonized by rhizobia, leading to a global impact on nitrogen fertilization of soils. In this process, rhizobia progress through root cortical and nodular cell layers via an infection thread, repeatedly passing host plasma membranes and cell walls. This study reveals the coordinated action of a symbiosis-specific pectin methyl esterase (SyPME1) and a nodulation pectate lyase (NPL) in mediating pectin alterations at the cell-cell interface, allowing bacteria to be temporarily released into and taken up from the subjacent cell, facilitating the successful intracellular progression of infection threads through the entire root cortical tissue.
Article
Biochemistry & Molecular Biology
Emilia Neuwirt, Giovanni Magnani, Tamara Cikovic, Svenja Woehrle, Larissa Fischer, Anna Kostina, Stephan Flemming, Nora J. Fischenich, Benedikt S. Saller, Oliver Gorka, Steffen Renner, Claudia Agarinis, Christian N. Parker, Andreas Boettcher, Christopher J. Farady, Rebecca Kesselring, Christopher Berlin, Rolf Backofen, Marta Rodriguez-Franco, Clemens Kreutz, Marco Prinz, Martina Tholen, Thomas Reinheckel, Thomas Ott, Christina J. Gross, Philipp J. Jost, Olaf Gross
Summary: The activation mechanisms of NOD-like receptor (NLR) protein-mediated inflammasomes were investigated. It was discovered that tyrosine kinase inhibitors (TKIs), including clinically approved drugs like imatinib and crizotinib, activated the NLRP3 inflammasome. These TKIs caused lysosomal swelling and damage, resulting in cell lysis and activation of NLRP3. This finding suggests a potential off-target effect of TKIs that may contribute to their clinical efficacy or adverse effects.
Article
Multidisciplinary Sciences
Jiahuan Chen, Zhijuan Wang, Lixiang Wang, Yangyang Hu, Qiqi Yan, Jingjing Lu, Ziyin Ren, Yujie Hong, Hongtao Ji, Hui Wang, Xinying Wu, Yanru Lin, Chao Su, Thomas Ott, Xia Li
Summary: The success of legumes relies on the formation and maintenance of optimal symbiotic nodules, which require a balance between symbiosis and plant growth. Cytokinin plays a key role in the regulation of nodulation, but its mechanism is not fully understood. In this study, the authors identified a response regulator called GmRR11d that mediates the systemic inhibition of nodulation. GmRR11d is induced by rhizobia and low levels of cytokinin, and it can suppress the transcriptional activity of GmNSP1 on GmNIN1a, leading to the inhibition of soybean nodulation. GmRR11d positively regulates cytokinin response and its binding on the GmNIN1a promoter is enhanced by cytokinin. Furthermore, the induction of GmRR11d by rhizobia and its function are dependent on GmNARK, a CLV1-like receptor kinase that inhibits nodule formation in shoots. Overall, GmRR11d controls a transcriptional program associated with nodulation attenuation and cytokinin response activation, which is essential for systemic regulation of nodulation.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Chao Su, Marta Rodriguez-Franco, Beatrice Lace, Nils Nebel, Casandra Hernandez-Reyes, Pengbo Liang, Eija Schulze, Evgeny V. Mymrikov, Nikolas M. Gross, Julian Knerr, Hong Wang, Lina Siukstaite, Jean Keller, Cyril Libourel, Alexandra A. M. Fischer, Katharina E. Gabor, Eric Mark, Claudia Popp, Carola Hunte, Wilfried Weber, Petra Wendler, Thomas Stanislas, Pierre-Marc Delaux, Oliver Einsle, Robert Grosse, Winfried Roemer, Thomas Ott
Summary: The organization of membranes in plants is mainly controlled by the cell wall and cytoskeleton, with some proteins, such as remorins, playing a role in protein and lipid organization. Symbiosis-specific SYMREM1 gene mutations disrupt membrane organization in root nodules. AlphaFold modeling and biochemical analyses reveal that SYMREM1 forms oligomers and may contribute to higher-order membrane scaffolding.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Bushra Saeed, Florian Deligne, Carla Brillada, Kai Duenser, Franck Aniset Ditengou, Ilona Turek, Alaa Allahham, Nenad Grujic, Yasin Dagdas, Thomas Ott, Juergen Kleine-Vehn, Gregory Vert, Marco Trujillo
Summary: Unlike other eukaryotic model organisms, the main sources of K63-linked Ub chains in Arabidopsis are UBC35 and UBC36. Previous research has associated K63-linked chains with vesicle trafficking regulation but lacked definitive evidence for their role in endocytosis. This study demonstrates that K63-Ub chains are essential for endocytic trafficking in plants and are also involved in selective autophagy through the interaction with NBR1. These findings highlight the importance of K63-Ub chains in maintaining proteostasis in plants.
Editorial Material
Plant Sciences
Jinhong Yuan, Thomas Ott, Andreas Hiltbrunner
TRENDS IN PLANT SCIENCE
(2023)
Article
Biology
Beatrice Lace, Chao Su, Daniel Invernot Perez, Marta Rodriguez-Franco, Tatiana Vernie, Morgane Batzenschlager, Sabrina Egli, Cheng-Wu Liu, Thomas Ott
Summary: Host-controlled intracellular accommodation of nitrogen-fixing bacteria is essential for the establishment of a functional Root Nodule Symbiosis (RNS). Comparative phylogenomic studies have identified RPG as a critical genetic determinant for bacterial infection. This study provides evidence that RPG is part of a protein complex involved in membrane polarization, cytoskeleton-mediated connectivity, and polar secretion during bacterial infection.
Article
Plant Sciences
Jesus Montiel, Dugald Reid, Thomas H. Gronbaek, Caroline M. Benfeldt, Euan K. James, Thomas Ott, Franck A. Ditengou, Marcin Nadzieja, Simon Kelly, Jens Stougaard
Summary: This study reports intercellular infection of the model legume Lotus japonicus by the IRBG74 strain and reveals the genetic requirements and differences in the pathways governing this process compared to intracellular infection. The results indicate a differential requirement for cytokinin signaling during intercellular rhizobial entry, highlighting distinct modalities of inter- and intracellular infection mechanisms in L. japonicus.
Article
Plant Sciences
Moira Giovannoni, Lucia Marti, Simone Ferrari, Natsuki Tanaka-Takada, Masayoshi Maeshima, Thomas Ott, Giulia De Lorenzo, Benedetta Mattei
Summary: The study reveals the crucial role of PCaP1 in plant immune responses triggered by OGs and flagellin, mainly impacting late responses and affecting the responsiveness of plants to a second treatment with OGs. Localization studies on PCaP1 post-OG treatment indicate its presence on the plasma membrane with specific structures, as well as its association with endocytic vesicles.
PLANT CELL AND ENVIRONMENT
(2021)