期刊
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
卷 145, 期 -, 页码 1-7出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2017.12.004
关键词
Bemisia tabaci; Glutamate-gated chloride channel; Double-stranded RNA; Abamectin; mRNA expression; Mortality
资金
- China Agriculture Research System [CARS-24-C-03]
- National Natural Science Foundation of China [31471773, 31601635]
- Beijing Nova Program [xx2018087]
- Beijing Academy of Agriculture and Forestry Sciences [QNJJ201610]
The Bemisia tabaci (Gennadius) cryptic species complex comprises very destructive insect pests of agricultural crops worldwide and has been found to be resistant to various insecticides in China. Abamectin is one of the most widely used insecticides for insect pest control and the glutamate-gated chloride channel (GIuCl) in insects was presumed to be the main target site of abamectin. In this study, a 1353 bp full-length cDNA encoding GIuCI (named BtGluCl, GenBank ID: MF673854) was cloned and characterized from B. tabaci. BtGluCl encodes 450 amino acids, which shares 71-81% identity with other insect GluCl isoforms. Spatial and temporal expression revealed BtGluCl was highly expressed in the 4th nymphal instar and adult head, and the least expressed in the 1st nymphal instar and adult leg. Dietary ingestion of dsBtGluCl significantly reduced the mRNA level of BtGluCl in the treated adults by 62.9% and greatly decreased abamectin-induced mortality. Thus, our results could be conducive to further understanding the mechanisms of resistance to abamectin in arthropods.
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