期刊
PARASITOLOGY
卷 146, 期 2, 页码 197-205出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182018001002
关键词
Culex pipiens pallens; deltamethrin resistance; miR-13664
类别
资金
- National Institutes of Health of USA (NIH) [2R01AI075746]
- National Natural Science Foundation of China [81772227, 81471984, 81672056, 81672058]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Extensive insecticide use has led to the resistance of mosquitoes to these insecticides, posing a major barrier to mosquito control. Previous Solexa high-throughput sequencing of Culex pipiens pallens in the laboratory has revealed that the abundance of a novel microRNA (miRNA), miR-13664, was higher in a deltamethrin-sensitive (DS) strain than a deltamethrin-resistant (DR) strain. Real-time quantitative PCR revealed that the miR-13664 transcript level was lower in the DR strain than in the DS strain. MiR-13664 oversupply in the DR strain increased the susceptibility of these mosquitoes to deltamethrin, whereas inhibition of miR-13664 made the DS strain more resistant to deltamethrin. Results of bioinformatic analysis, quantitative reverse-transcriptase polymerase chain reaction, luciferase assay and miR mimic/inhibitor microinjection revealed CpCYP314A1 to be a target of miR-13664. In addition, downregulation of CpCYP314A1 expression in the DR strain reduced the resistance of mosquitoes to deltamethrin. Taken together, our results indicate that miR-13664 could regulate deltamethrin resistance by interacting with CpCYP314A1, providing new insights into mosquito resistance mechanisms.
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