Review
Biochemistry & Molecular Biology
Monika Baczkowska, Magdalena Zgliczynska, Jan Faryna, Ewa Przytula, Blazej Nowakowski, Michal Ciebiera
Summary: Placental abruption, a rare perinatal complication, involves the separation of the placenta from the uterus lining before childbirth, posing serious consequences and a high risk of maternal and fetal mortality. Disruption of immunological processes on the maternal-fetal interface plays a crucial role in the pathophysiology of this condition, requiring further research to fully understand this complex process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Diogo Henrique Kita, Gisele Alves de Andrade, Juliana Morais Missina, Kahoana Postal, Viktor Kalbermatter Boell, Francielli Sousa Santana, Ingrid Fatima Zattoni, Isadora da Silva Zanzarini, Vivian Rotuno Moure, Fabiane Gomes de Moraes Rego, Geraldo Picheth, Emanuel Maltempi de Souza, David A. Mitchell, Suresh Ambudkar, Giovana Gioppo Nunes, Glaucio Valdameri
Summary: The study found that polyoxovanadates can serve as inhibitors of ABC drug transporters, selectively inhibiting P-glycoprotein with V-10 and V-18 being the most promising compounds.
Article
Infectious Diseases
Bo Yang, Dongqiang Wang, Mingxiao Liu, Xiaodong Wu, Jigang Yin, Guan Zhu
Summary: The study aimed to generate host cells with resistance to specific anti-cryptosporidial drugs by transiently overexpressing the MDR1 gene. Results showed increased drug tolerance in host cells for certain compounds with varying effects on the anti-parasitic efficacy, highlighting the importance of understanding drug targets in parasite vs. host cells.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Microbiology
Jan Viglas, Petra Olejnikova
Summary: The ABC transporter superfamily plays a crucial role in transporting various molecules across biological membranes, including antifungal compounds in filamentous fungi. Understanding the physiological substrates and roles of individual subfamilies of ABC transporters in fungi is important for clarifying their significance in clinical and agricultural settings.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Physical
Damian Krzyzanowski, Marcin Kruszewski, Agnieszka Grzelak
Summary: Silver nanoparticles (AgNPs) have unique properties that have attracted significant attention, showing antibacterial, antifungal, and anticancer properties. However, they can also exhibit cytotoxic effects and modulate the activity and expression of ABC transporters.
Article
Biology
Yali Shan, Shiping Shen, Jing Long, Zhengshan Tang, Cichun Wu, Xin Ni
Summary: The study reveals that there is an increase in inflammatory response, particularly from macrophages, during term and preterm labor. This inflammation plays a critical role in the initiation of parturition.
Review
Cell Biology
Lobke M. Gierman, Gabriela B. Silva, Zahra Pervaiz, Johanne J. Rakner, Siv B. Mundal, Astrid J. Thaning, Ingunn Nervik, Mattijs Elschot, Seema Mathew, Liv Cecilie V. Thomsen, Line Bjorge, Ann-Charlotte Iversen
Summary: In preeclampsia, the placental intravillous TLR3 expression is significantly increased, while no significant increase is observed in the decidua. The functionality of TLR3 was confirmed in both the decidua and placenta, but the expression levels did not correlate between the two sites.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ludwik Gorczyca, Jianyao Du, Kristin M. Bircsak, Xia Wen, Anna M. Vetrano, Lauren M. Aleksunes
Summary: The study revealed that low oxygen concentration differentially regulates transporter expression in human placental models, leading to both upregulation and downregulation of specific transporters. Additionally, transcriptional regulators of transporters were found to be affected by hypoxia. This suggests that oxygen concentration plays a role in regulating transporter expression across experimental human placental models.
Article
Cell Biology
Kelly J. Baines, Michelle S. Klausner, Violet S. Patterson, Stephen J. Renaud
Summary: This study identified osteopontin as a factor produced by NK cells that may contribute to the regulation of trophoblast invasion at the maternal-fetal interface in rats. Osteopontin was found to be present in the decidua and adjacent to the primitive placenta in wild-type rats, colocalizing with the NK cell marker perforin. However, in IL15?/? rats lacking NK cells, osteopontin and perforin were not readily detectable in the decidua, despite high levels of osteopontin in uterine glands. Neutralization of osteopontin decreased invasion of rat trophoblasts, suggesting its potential role in modulating trophoblast invasion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Gao-Jie Ye, Chao-Yun Cai, Zhuo-Xun Wu, Qiu-Xu Teng, Jing-Quan Wang, Zhe-Sheng Chen, Bo Wang
Summary: Three series of phenylurea indole derivatives were synthesized with potent inhibitory activities on ABCG2. Four phenylurea indole derivatives with extended 7C system showed the most potent ABCG2 inhibition and no inhibition on ABCB1. Further investigation on two compounds revealed that they increased the accumulation of mitoxantrone in ABCG2-overexpressing cells and stimulated the ATP hydrolysis of ABCG2 transporter.
BIOORGANIC CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Lara Garcia-Varela, Manuel Rodriguez-Perez, Antia Custodia, Rodrigo Moraga-Amaro, Nicola A. Colabufo, Pablo Aguiar, Tomas Sobrino, Rudi A. J. O. Dierckx, Aren van Waarde, Philip H. Elsinga, Gert Luurtsema
Summary: P-glycoprotein (P-gp) inducer MC111 increases P-gp functionality in the whole brain of rats, potentially offering a treatment option for neurodegenerative diseases.
MOLECULAR PHARMACEUTICS
(2021)
Article
Oncology
Lucia Pavlikova, Mario Seres, Albert Breier, Zdena Sulova
Summary: This paper provides an overview of the possible role of miRNAs in the molecular mechanisms that lead to multidrug resistance (MDR). miRNAs regulate post-transcriptional processes in the proteosynthetic pathway and may serve as specific effectors or interesting targets for cancer therapy.
Article
Biochemistry & Molecular Biology
Bianca Reis Santos, Jeane Martinha dos Anjos Cordeiro, Luciano Cardoso Santos, Larissa da Silva Santana, Acacia Eduarda de Jesus Nascimento, Juneo Freitas Silva
Summary: This study aimed to evaluate whether hypothyroidism activates the inflammasome-NLRP3 pathway and pyroptosis at the maternal-fetal interface of rats and whether kisspeptin can modulate these processes. The findings demonstrated that maternal hypothyroidism activated the inflammasome-NLRP3 pathway and pyroptosis at the maternal-fetal interface of rats and that treatment with Kp10 was able to block these processes, thus suggesting that kisspeptin analogues may be promising in the treatment of gestational diseases that involve inflammasome activation and pyroptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chao-Yun Cai, Qiu-Xu Teng, Megumi Murakami, Suresh V. Ambudkar, Zhe-Sheng Chen, Vijaya L. Korlipara
Summary: A series of 22 quinazolinamine derivatives with potent inhibitory activities on BCRP and P-gp were synthesized. Compound 22 was identified as a dual BCRP and P-gp inhibitor, while compound 33 showed BCRP inhibitory activity. These compounds changed the localization of BCRP and P-gp, inhibiting the efflux of anticancer drugs by the two ABC transporters. Compounds 22 and 33 also stimulated ATP hydrolysis, increasing the accumulation of mitoxantrone in BCRP-overexpressing cells.
Article
Oncology
Raul Munoz Velasco, Paula Jimenez Sanchez, Ana Garcia Garcia, Raquel Blanco Martinez-Illescas, Angela Pastor Senovilla, Marian Lozano Yague, Alfonsina Trento, Rosa Maria Garcia-Martin, Diego Navarro, Bruno Sainz Jr, Jose Luis Rodriguez Peralto, Victor Javier Sanchez-Arevalo Lobo
Summary: This study explores the role of the chromatin remodeler BPTF in pancreatic ductal adenocarcinoma (PDA). The findings suggest that reducing BPTF levels can inhibit cell proliferation and migration in PDA, as well as enhance the therapeutic effect of gemcitabine. The study also reveals that BPTF is required for c-MYC recruitment to the promoter of ABC-transporters, which affects gemcitabine accumulation in tumor cells and DNA damage.
Review
Obstetrics & Gynecology
Elizabeth Asztalos, Kellie E. Murphy, Stephen G. Matthews
Summary: Synthetic antenatal corticosteroids are commonly used in women at risk for preterm birth before 34 weeks of gestation, but there are concerns about their potential long-term effects on the fetal brain. Further research is needed to gather sufficient safety data, both short term and long term. Caution should be exercised in the use of antenatal corticosteroids until dosing strategies can be optimized and better identification of women at risk of preterm birth is achieved.
AMERICAN JOURNAL OF PERINATOLOGY
(2022)
Review
Obstetrics & Gynecology
Lilian M. Martinelli, Antonio Carucci, Victor J. H. Payano, Kristin L. Connor, Enrrico Bloise
Summary: The yolk sac (YS) is an important extraembryonic membrane in vertebrates. This review compares the developmental aspects and functions of the YS in humans and mice, and highlights the similarities and differences between the two species. Despite some dissimilarities, the mouse can potentially serve as a translational model for studying the human YS.
REPRODUCTIVE SCIENCES
(2023)
Article
Pediatrics
Jennifer M. Lye, Julia A. Knight, Jasleen Arneja, Ryan A. Seeto, Jody Wong, Nadya Adel Khani, Jennifer D. Brooks, Robert D. Levitan, Stephen G. Matthews, Stephen J. Lye, Rayjean J. Hung
Summary: This study aimed to investigate the relationship between maternal acetaminophen use during pregnancy and cognitive development at 4 years old. Through data analysis and regression models, the study found no association between acetaminophen use and cognitive development in offspring.
PEDIATRIC RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kristin L. Connor, Enrrico Bloise, Todd Z. DeSantis, Stephen J. Lye
Summary: This study explores the effects of malnutrition on gut structure and function in pregnant women and highlights the adaptive changes in the gut holobiont in response to suboptimal maternal diets. Specifically, a high-fat diet increases maternal small intestinal architecture and proinflammatory load while decreasing the expression of P-glycoprotein. On the other hand, undernutrition increases small intestinal villi proliferation and the expression of certain MDR transporters, but decreases the expression of Abcg2. Furthermore, specific microbial taxa are significantly associated with the levels of MDR transporter expression.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Review
Obstetrics & Gynecology
Elaine Sousa, Simone F. Nery, Maira Casalechi, Luiz C. Thimoteo, Sara P. C. Paiva, Agnaldo L. Silva-Filho, Fernando M. Reis
Summary: Several studies have shown that 'stress' is a significant factor leading to discontinuation of assisted reproductive technology (ART) treatment, but the prevalence and characteristics of stressors and stress responses in this context remain unclear. This systematic review evaluated the perception and reported causes of 'stress' among couples who discontinued ART treatment. The results showed that stress was reported by approximately 30.9% of participants as a reason for ART discontinuation, with factors such as prognosis, treatment discomfort, family demands, time pressure, and economic burden contributing to stress.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Pharmacology & Pharmacy
C. B. V. Andrade, L. V. A. Lopes, T. M. Ortiga-Carvalho, S. G. Matthews, E. Bloise
Summary: This review comprehensively examines the effect of infection on the placental multidrug resistance (MDR) transporters P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP), finding that infection can regulate the expression of P-gp and BCRP in the placenta by altering their mRNA, protein, and function levels. The modulation effect varies according to different infection factors, gestational age, trophoblast type, and species. The expression and localization of Toll-like receptor (TLR) proteins at the maternal-fetal interface are also discussed, aiming to understand how infective agents modulate placental MDR. The alterations in placental MDR induced by infection may impact the distribution of important MDR substrates and adversely affect pregnancy outcome and maternal/neonatal health.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2023)
Article
Neurosciences
Phetcharawan Lye, Enrrico Bloise, Stephen G. G. Matthews
Summary: Infectious pathogen-associated molecular patterns (PAMPs) significantly modify the expression and function of multidrug resistance (MDR) transporters in human fetal brain endothelial cells (hfBECs), potentially leading to altered trajectories of fetal brain development.
FLUIDS AND BARRIERS OF THE CNS
(2023)
Article
Cell Biology
Victor Machado de Mello Andrade, Amanda Fernandes de Moura, Katlen da Costa Chaves, Camilla Pereira Dias da Rocha, Cherley Borba Vieira de Andrade, Isis Hara Trevenzoli, Tania Maria Ortiga-Carvalho, Luciane Claudia Barcellos, Mario Vaisman, Veronica Pinto Salerno
Summary: By studying a rat model of ovariectomy, it was found that consumption of Paraguariensis tea can reduce weight gain and decrease levels of triglycerides, total cholesterol, and LDL in the blood. In addition, tea consumption can decrease the area of adipocytes and increase the content of UCP1 and SIRT1 in adipose tissue.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Article
Anatomy & Morphology
Everton Luis dos Santos Cardoso, Fabio Cahue, Iordan Emanuel Ferreira Miranda, Marcelo de Lima Sant'Anna, Cherley Borba Vieira Andrade, Raiana Andrade Quintanilha Barbosa, Tania Maria Ortiga-Carvalho, Mario Vaisman, Veronica Pinto Salerno
Summary: High caloric intake and physical inactivity are known precursors to chronic metabolic diseases. High Intensity Intermittent Exercise (HIIE) and Intermittent Fasting (IF) can attenuate their negative effects by improving metabolism. In a study on rats, the combined effect of HIIE and IF showed improved performance and modified white adipose tissue metabolism through the browning process.
Article
Virology
Victor Jesus Huaringa Payano, Lara Veronica de Araujo Lopes, Larissa Rodrigues Peixoto, Keila Alves da Silva, Tania Maria Ortiga-Carvalho, Alexandre Tafuri, Annamaria Ravara Vago, Enrrico Bloise
Summary: The activin-follistatin system plays an important role in the regulation of cervical lesions, as indicated by the immunostaining of beta A-activin and follistatin. The immunostaining of cytoplasmic beta A-activin and follistatin was higher than nuclear immunostaining in all cervical epithelium layers of the CIN1, CIN2, CIN3, and SCC groups. A significant decrease in the cytoplasmic and nuclear immunostaining of beta A-activin was detected in all cervical epithelial layers from the control to the CIN1, CIN2, CIN3, and SCC groups. Only nuclear follistatin immunostaining exhibited a significant reduction in specific epithelial layers of cervical tissues from CIN1, CIN2, CIN3, and SCC compared to the control.
Review
Microbiology
Thaina Ferraz, Samantha J. Benton, Israa Zareef, Oluwatomike Aribaloye, Enrrico Bloise, Kristin L. Connor
Summary: Obesity is a risk factor for severe COVID-19 disease during pregnancy. The co-occurrence of high maternal BMI and gestational SARS-CoV-2 infection are detrimental to fetoplacental development. In pregnancies with high maternal BMI and SARS-CoV-2 infection, chronic inflammation, fetal vascular malperfusion, maternal vascular malperfusion, and fibrinoids were commonly observed placental lesions.
Article
Obstetrics & Gynecology
Fernando M. Reis, Sandrine Chouzenoux, Mathilde Bourdon, Mohamed Jeljeli, Charles Chapron, Frederic Batteux
Summary: Endometriotic cells from patients with endometriosis have higher ability to proliferate, produce reactive oxygen species (ROS), and secrete proinflammatory cytokines compared to endometrial cells from controls. Treatment with ulipristal acetate (UPA) stimulates proliferation and ROS production in endometriotic cells but fails to reduce the excessive secretion of proinflammatory cytokines.←
REPRODUCTIVE SCIENCES
(2023)
Article
Obstetrics & Gynecology
Hafsah Mughis, Phetcharawan Lye, Stephen G. Matthews, Enrrico Bloise
Summary: This study found that human fetal brain endothelial cells express SARS-CoV-2-associated cell entry proteins during early and mid-pregnancy. Hypoxia can modify the expression levels of these proteins in a gestational age and time-of-exposure-dependent manner.
AMERICAN JOURNAL OF OBSTETRICS & GYNECOLOGY MFM
(2023)
Article
Biology
F. Aguiar, P. Rhana, E. Bloise, C. B. Nunes, A. L. Rodrigues, E. Ferreira
Summary: The protein expression and subcellular localization of T-type voltage-dependent Ca2+ channels (Cav3) were evaluated in human breast tissue samples. It was found that the nuclear expressions of CaV3.1, CaV3.2, and CaV3.3 decreased in advanced stages of neoplastic transformation, while the cytoplasmic expression of CaV3.1 and CaV3.2 increased. The change in protein expression and subcellular localization of CaV3 corresponded with the neoplastic transformation stages, suggesting a possible involvement of CaV3 in the carcinogenic processes and its potential as a pharmacological target in breast cancer treatment.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)