期刊
NUCLEIC ACIDS RESEARCH
卷 46, 期 15, 页码 7522-7532出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gky665
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资金
- National Natural Science Foundation of China [21472197, 21675162, 21778058]
- Beijing Natural Science Foundation [7182189]
- Joint Funds of the National Natural Science Foundation of China [U1432250]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA09030307]
G-quadruplex DNA has been viewed as a prospective anti-cancer target owing to its potential biological relevance. Real-time monitoring of DNA G-quadruplex structures in living cells can provide valuable insights into the relationship between G-quadruplex formation and its cellular consequences. However, the probes capable of detecting DNA G-quadruplexes in living cells are still very limited. Herein, we reported a new fluorescent probe, IMT, for real-time visualization of DNA G-quadruplex structures in living cells. Using IMT as a fluorescent indicator, the quantity changes of DNA G-quadruplex at different points in time during continuous cellular progression responding to Aphidicolin and Hydroxyurea treatment have been directly visualized. Our data demonstrate that IMT will be a valuable tool for exploring DNA G-quadruplexes in live cells. Further application of IMT in fluorescence imaging may reveal more information on the roles of DNA G-quadruplexes in biological systems.
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