期刊
NUCLEIC ACIDS RESEARCH
卷 46, 期 14, 页码 7418-7424出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gky534
关键词
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资金
- National Natural Science Foundation of China [21672212, 31470783, 21432008]
- National Natural Science Foundation of China
DNA supercoiling is an important regulator of gene activity. The transmission of transcription-generated supercoiling wave along a DNA helix provides a way for a gene being transcribed to communicate with and regulate its neighboring genes. Currently, the dynamic behavior of supercoiling transmission remains unclear owing to the lack of a suitable tool for detecting the dynamics of supercoiling transmission. In this work, we established a torsion sensor that quantitatively monitors supercoiling transmission in real time in DNA. Using this sensor, we studied the transmission of transcriptionally generated negative supercoiling in linear and multi-way DNA duplexes. We found that transcription-generated dynamic supercoiling not only transmits along linear DNA duplex but also equally diverges at and proceeds through multi-way DNA junctions. We also show that such a process is regulated by DNA protein interactions and non-canonical DNA structures in the path of supercoiling transmission. These results imply a transcription-coupled mechanism of dynamic supercoiling-mediated intra-and inter chromosomal signal transduction pathway and their regulation in DNA.
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