Article
Biochemistry & Molecular Biology
Yun-Mi Jeong, Tae-Ik Choi, Kyu-Seok Hwang, Jeong-Soo Lee, Robert Gerlai, Cheol-Hee Kim
Summary: Recent advancements in optogenetics have allowed researchers to manipulate the olfactory system in zebrafish, revealing that light stimulation can activate olfactory neurons. Zebrafish larvae exhibit a preference for light over aversive odors when presented with conflicting sensory cues, showcasing the importance of olfaction in decision-making processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Optics
Himanshu Bansal, Gur Pyari, Sukhdev Roy
Summary: The fundamental process of information processing and memory formation in the brain is associated with complex neuron firing patterns, which can be precisely manipulated using light pulses in optogenetics. This study presents a detailed theoretical analysis of the temporal shaping of light pulses and demonstrates that non-square waveforms generate more naturalistic spiking patterns compared to traditional square pulses. These findings contribute to the development of new optogenetic strategies for simulating and manipulating neural activity patterns in the brain.
Article
Neurosciences
Gur Pyari, Himanshu Bansal, Sukhdev Roy
Summary: The main challenge in cardiac optogenetics is to achieve low-power, high-fidelity deep excitation of cells. Recent research has discovered new optogenetic proteins, ChRmine and bReaChES, which can enable safe sustained excitation of deeply situated cardiac cells, providing important implications for cardiac rhythm regulation and disease treatment.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Biochemistry & Molecular Biology
Akito Hatakeyama, Eriko Sugano, Tatsuki Sayama, Yoshito Watanabe, Tomoya Suzuki, Kitako Tabata, Yuka Endo, Tetsuya Sakajiri, Tomokazu Fukuda, Taku Ozaki, Hiroshi Tomita
Summary: Channelrhodopsins have been used in gene therapy to restore vision in patients with retinitis pigmentosa. The channel kinetics of ComV1 variants with different amino acid residues at the 172nd position were investigated. The study found that the characteristics of the amino acid at this position affect the radius of the ion gate, thereby altering the photocurrent and channel kinetics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Saeyeong Jeon, Youjin Lee, Daeho Ryu, Yoon Kyung Cho, Yena Lee, Sang Beom Jun, Chang-Hyeon Ji
Summary: Optogenetics has become crucial in neuroscience research due to its cell-type-specific neuromodulation feature. Technological advances in light delivery devices have allowed for the combination of optogenetics and electrophysiology, as demonstrated by a novel optrode array design for optical modulation and electrophysiological recording. In vivo experiments with transgenic mice expressing channelrhodopsin have confirmed the effectiveness of optical activation and neural recording.
Article
Medicine, Research & Experimental
Markus Vogt, Benjamin Schulz, Ahmed Wagdi, Jan Lebert, Gijsbert J. van Belle, Jan Christoph, Tobias Bruegmann, Robert Patejdl
Summary: This study demonstrates the direct optogenetic stimulation of smooth muscle cells to control gastric contractility, showing promising potential for restoring motility in gastroparesis in the future.
Review
Biochemistry & Molecular Biology
Kotaro Yamashiro, Yuji Ikegaya, Nobuyoshi Matsumoto
Summary: The complexity of brain functions requires precise manipulation of specific neuronal subtypes, which can be achieved through the combination of in utero electroporation and optogenetics. This method provides high spatiotemporal resolution control to study the functions and characteristics of specific regions, layers, and cell types.
Article
Cell Biology
Yi-Shyun Lai, Ya-Han Chang, Yong-Yi Chen, Jixuan Xu, Chi-Sian Yu, Su-Jing Chang, Pai-Sheng Chen, Shaw-Jenq Tsai, Wen-Tai Chiu
Summary: This study demonstrates the integral role of Ca2+ signals in cell biology, and optogenetic technology can be used to modulate Ca2+ waves and activate specific signaling pathways to explore the regulation of cell migration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Phillip Wright, Jessica Rodgers, Jonathan Wynne, Paul N. Bishop, Robert J. Lucas, Nina Milosavljevic
Summary: The study evaluated the safety of viral gene delivery of human rod opsin and two channelrhodopsins in ON bipolar cells of the mouse retina. The results showed that delivery of these opsins did not cause significant changes in retinal health or impact the survival of targeted cells. Additionally, there was no increase in retinal inflammation in treated mice compared to controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Anirudh R. Acharya, Lars Emil Larsen, Jean Delbeke, Wytse J. Wadman, Kristl Vonck, Alfred Meurs, Paul Boon, Robrecht Raedt
Summary: This study investigated the inhibitory effect of activating stGtACR2 on epileptic afterdischarges in the dentate gyrus. It was found that activation of stGtACR2 only reduced the line length of local field potential during illumination, but did not decrease the duration of afterdischarges.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Lakshmini Balachandar, Diana Borrego, Jorge Riera Diaz
Summary: Optogenetics is a modern technique that can be used for gene delivery in non-neuronal cell types, including astrocytes. In the rat brain, AAV serotype 8 has been shown to exhibit the most promising transduction patterns in astrocytes, which is important for the future application of optogenetics.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Neurosciences
Savitha Sridharan, Marta A. Gajowa, Mora B. Ogando, Uday K. Jagadisan, Lamiae Abdeladim, Masato Sadahiro, Hayley A. Bounds, William D. Hendricks, Toby S. Turney, Ian Tayler, Karthika Gopakumar, Ian Anton Oldenburg, Stephen G. Brohawn, Hillel Adesnik
Summary: Structure-guided mutagenesis has been used to engineer new optogenetic tools with high potency and fast kinetics, enabling precise control of population neural activity at a large scale, and temporally and spatially. These new tools have been extensively benchmarked and characterized, providing a resource for patterned optogenetics experiments. Furthermore, when combined with optimized procedures for holographic photostimulation, these new tools allow for the coactivation of hundreds of spatially defined neurons simultaneously with a single hologram.
Article
Multidisciplinary Sciences
Shoko Hososhima, Shinji Ueno, Satoshi Okado, Ken-ichi Inoue, Masae Konno, Yumeka Yamauchi, Keiichi Inoue, Hiroko Terasaki, Hideki Kandori, Satoshi P. Tsunoda
Summary: In this study, the researchers investigated the ion channel properties of five light-gated cation channelrhodopsins (GtCCR1-5) from the cryptophyte algae Guillardia theta. They compared these CCRs with ChR2 and other widely used channelrhodopsins in optogenetics. The results showed that GtCCR4 exhibited high light sensitivity and temporal precision, making it a promising candidate for optogenetic neuronal activation.
SCIENTIFIC REPORTS
(2023)
Article
Neurosciences
Daniel J. Rindner, Gyorgy Lur
Summary: The ability to control synaptic communication is essential in modern neuroscience. Recent advancements in protein engineering and screening have greatly expanded the optogenetic toolkit, allowing for multicolor approaches in studying neural circuits. However, the scarcity of opsins with truly discrete spectra poses challenges in avoiding unintended cross-activation of optogenetic tools (crosstalk).
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Cell Biology
Liying Zhang, Kaituo Wang, Shuo Ning, Per Amstrup Pedersen, Annette Susanne Duelli, Pontus Emanuel Gourdon
Summary: In this study, the production and purification procedure of the D156C form of ChR2, which exhibits prolonged channel opening, was reported. Furthermore, conditions for growing ChR2 crystals that scatter X-rays to 6 angstrom were developed. The results provide a foundation for future structural studies of ChR2 in different states.
Article
Substance Abuse
Paula A. Zamudio-Bulcock, Gregg E. Homanics, John J. Woodward
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2018)
Article
Neurosciences
Wesley N. Wayman, John J. Woodward
JOURNAL OF NEUROSCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Sudarat Nimitvilai, Marcelo F. Lopez, John J. Woodward
Article
Neurosciences
Kevin M. Braunscheidel, Michael P. Okas, Michaela Hoffman, Patrick J. Mulholland, Stan B. Floresco, John J. Woodward
JOURNAL OF NEUROSCIENCE
(2019)
Article
Substance Abuse
Paula A. Zamudio, Thetford C. Smothers, Gregg E. Homanics, John J. Woodward
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
(2020)
Article
Neurosciences
Reginald Cannady, Sudarat Nimitvilai-Roberts, Sarah D. Jennings, John J. Woodward, Patrick J. Mulholland
Article
Neurosciences
Paula A. Zamudio, Dominic A. Gioia, Marcelo Lopez, Gregg E. Homanics, John J. Woodward
Summary: This study investigated the impact of NMDARs on ethanol consumption and behavioral adaptations during the development of alcohol dependence. The results suggest that specific subunits of NMDARs may play a critical role in the escalation of ethanol intake in alcohol dependence.
PSYCHOPHARMACOLOGY
(2021)
Article
Neurosciences
D. A. Gioia, J. J. Woodward
Summary: The lateral orbitofrontal cortex (LOFC) encodes information related to rewarding substances and is crucial for flexible decision-making. Chronic intermittent ethanol (CIE) exposure alters LOFC neuronal activity during alcohol consumption, leading to increased ethanol intake and aversion resistance.
Article
Neurosciences
Todd B. Nentwig, Dylan T. Vaughan, Kevin M. Braunscheidel, Brittney D. Browning, John J. Woodward, L. Judson Chandler
Summary: In male rats, the lateral habenula (LHb) is not necessary for the acquisition or expression of escalation of ethanol consumption or expression of somatic symptoms of ethanol withdrawal.
NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Neurosciences
Sudarat Nimitvilai-Roberts, Dominic Gioia, Marcelo F. Lopez, Christina M. Glaser, John J. Woodward
Summary: Alcohol use disorder is associated with altered neuron function in orbitofrontal cortex (OFC) and basolateral amygdala (BLA), which send inputs to dorsal striatum (DS). Chronic intermittent exposure to ethanol alters electrophysiological properties of OFC and BLA neurons. DS-projecting OFC neurons become hyperexcitable 3- and 7-days following ethanol exposure, while DS-projecting BLA neurons show decreased firing at 3-days withdrawal and increased firing at 7- and 14-days. CIE exposure enhances the amplitude and frequency of spontaneous excitatory postsynaptic currents (sEPSCs) in DS-projecting OFC neurons.
Article
Neurosciences
Elizabeth J. Glover, E. Margaret Starr, Andres Gascon, Kacey Clayton-Stiglbauer, Christen L. Amegashie, Alyson H. Selchick, Dylan T. Vaughan, Wesley N. Wayman, John J. Woodward, L. Judson Chandler
Summary: This study provides a detailed analysis of cortical input to the RMTg in male rats, revealing a prominent cortico-subcortical projection involved in adaptive behavioral responding to aversive stimuli. The findings lay the foundation for future research exploring alterations in circuit function in diseases characterized by deficits in cognitive control over reward and aversion.
NEUROPSYCHOPHARMACOLOGY
(2023)
Article
Neurosciences
Paula A. Zamudio, Dominic Gioia, Christina Glaser, John J. Woodward
Summary: The cerebellum, especially the posterior region, plays a crucial role in cognitive flexibility and regulation of alcohol consumption.
Article
Neurosciences
Philip O'Herron, Manuel Levy, John J. Woodward, Prakash Kara
Correction
Neurosciences
Lucia Privitera, Ellen L. Hogg, Matthias Gaestel, Mark J. Wall, Sonia A. L. Correa
Article
Neurosciences
Li-Ya Jiang, Guan-Hao Wang, Jing-Jiao Xu, Xiao-Li Li, Xiao-Yan Lin, Xiang Fang, Hong-Xu Zhang, Mei Feng, Chun-Ming Jiang
Summary: This study reveals the importance of LINC00473 in regulating temozolomide (TMZ) resistance in glioblastoma (GB) and its potential mechanism. By regulating the expression of CEBP alpha and MGMT, LINC00473 promotes the formation of chemoresistance. Furthermore, LINC00473 can transfer chemoresistance to adjacent sensitive cells through exosomes.
Article
Neurosciences
Olga Kopach, Tetyana Pivneva, Nataliya Fedirko, Nana Voitenko
Summary: This study found that diabetic animals exhibit severe xerostomia characterized by reduced saliva flow rate, diminished total protein content, and decreased amylase activity. The impaired saliva production in diabetes is associated with reduced and delayed intracellular Ca2+ signals in submandibular acinar cells, caused by malfunctioning mitochondria. Targeting malfunctioning mitochondria may be a potential strategy for the treatment of diabetic xerostomia.
Article
Neurosciences
Nicholas M. Timme, Cherish E. Ardinger, Seth D. C. Weir, Rachel Zelaya-Escobar, Rachel Kruger, Christopher C. Lapish
Summary: This study aimed to assess aversion-resistant drinking behavior in head-fixed mice and explore the relationship between non-consummatory behaviors and aversion-resistant drinking. The results showed that head-fixed mice exhibited heterogenous levels of aversion-resistant drinking and non-consummatory behaviors were related to the intensity of this behavior.
Article
Neurosciences
David R. Maguire, Charles P. France
Summary: Methocinnamox (MCAM) is a novel, long-acting opioid receptor antagonist that effectively decreases fentanyl self-administration and prevents opioid overdose in monkeys. The study demonstrates the potential therapeutic utility of MCAM in the treatment of opioid use disorder.
Article
Neurosciences
Xiang Li, Dan Feng, Shenglu Ma, Mingxing Li, Shulei Zhao, Man Tang
Summary: This study investigated the effects of fluoxetine on neurochemical, neurobiological, and neurobehavioral changes in different subregions of the hippocampus. The results showed that fluoxetine increased dialysate 5-HT, decreased membrane 5-HTT protein, and increased cytoplasmic fraction. Additionally, fluoxetine reduced immobility times in behavioral tests, with greater effects observed in the ventral subregion compared to the dorsal subregion.
Article
Neurosciences
Alexander V. Zholos, Mariia I. Melnyk, Dariia O. Dryn
Summary: Acetylcholine is an important neurotransmitter in visceral smooth muscles, activating M2 and M3 muscarinic receptors to cause smooth muscle excitation and contraction. This review focuses on the cellular and molecular mechanisms underlying acetylcholine-induced depolarisation and smooth muscle contraction, as well as the effects of anticholinergic drugs on gastrointestinal motility. The knowledge gained from recent studies has greatly expanded our understanding of these processes.
Article
Neurosciences
Zhenlong Li, Hsien-Yu Peng, Chau-Shoun Lee, Tzer-Bin Lin, Ming-Chun Hsieh, Cheng-Yuan Lai, Han-Fang Wu, Lih-Chyang Chen, Mei-Ci Chen, Dylan Chou
Summary: Methylone shows significant efficacy in treating depression and social deficits, making it an ideal candidate for anti-depressant medication.
Article
Neurosciences
Aline Freyssin, Allison Carles, Sarra Guehairia, Gilles Rubinstenn, Tangui Maurice
Summary: This study explores the potential of combining FENM and S1R agonists in the treatment of Alzheimer's disease. The results showed that most FENM-based combinations can protect against learning deficits caused by A beta 25-35, with better efficacy in short-term memory.
Article
Neurosciences
J. D. Lorente, J. Cuitavi, L. Rullo, S. Candeletti, P. Romualdi, L. Hipolito
Summary: This study analyzed the effects of pain on negative affect in different sexes and time courses, as well as the involvement of the dynorphinergic and corticotropin releasing factor systems in these pain-related behaviors. The results showed sex and time-dependent anxiety- and anhedonia-like behaviors induced by pain in female rats. The recruitment of KOR/DYN in the NAc was identified as a key neurological substrate mediating pain-induced behavioral alterations.
Article
Neurosciences
Rongjun Liu, Daofan Sun, Xiuzhong Xing, Qingge Chen, Bo Lu, Bo Meng, Hui Yuan, Lan Mo, Liufang Sheng, Jinwei Zheng, Qiusheng Wang, Junping Chen, Xiaowei Chen
Summary: The coexistence of pain and depression is frequently observed in patients with chronic pain and depression. Oxytocin, a neuropeptide, has been reported to relieve chronic pain and depressive symptoms. This study investigated the effect of intranasal oxytocin on neuropathic pain and comorbid depressive symptoms, and found that oxytocin attenuated depression-like behavior but did not alleviate mechanical hyperalgesia. The results suggest that intranasal oxytocin may have the potential to treat depressive symptoms in neuropathic pain patients.