期刊
NATURE METHODS
卷 15, 期 8, 页码 598-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0045-8
关键词
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资金
- Deutsche Forschungsgemeinschaft (DFG) Collaborative Research Center [SFB1036]
- Weizmann Institute of Science
- China Scholarship Council
- HBIGS graduate school
- Alexander von Humboldt Foundation
- DFG [KN498/11-1]
- Israel Science Foundation [1775/12, 2179/14]
- HFSP Career Development Award [CDA00077/2015]
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins.
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