Article
Multidisciplinary Sciences
Anna S. Dickson, Tekle Pauzaite, Esther Arnaiz, Brian M. Ortmann, James A. West, Norbert Volkmar, Anthony W. Martinelli, Zhaoqi Li, Niek Wit, Dennis Vitkup, Arthur Kaser, Paul J. Lehner, James A. Nathan
Summary: Researchers have discovered an oxygen-sensitive pathway that regulates cholesterol synthesis in human cells, which involves the degradation of SREBP2 under hypoxic conditions. This pathway overrides the normal response to sterol levels and is independent of HIF. The degradation of SREBP2 in hypoxia protects cells from statin-induced cell death and leads to a dependence on exogenous cholesterol uptake, shedding light on the auxotrophic nature of many solid organ tumors for cholesterol.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Zhi-Wen Qiao, Xing-Long Ji, Hong-Liang Li, Xun Wang, Chun-Ling Zhang, Xiao-Fei Wang, Chun-Xiang You
Summary: The study of the MdARF8 gene in apple reveals its involvement in the regulation of plant growth and development through jasmonate signaling. Ectopic expression in Arabidopsis showed that MdARF8 reduces the sensitivity to MeJA and alleviates leaf senescence and taproot elongation inhibition. MeJA treatment partially recovers the dysplastic phenotype of transgenic Arabidopsis root hair. This study provides valuable insights into the functional characterization of ARF8 and the crosstalk between jasmonate and auxin in apple.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Multidisciplinary Sciences
Feng Li, Michael Lee, Caroline Esnault, Katie Wendover, Yabin Guo, Paul Atkins, Mikel Zaratiegui, Henry L. Levin
Summary: Retroelements can retain insertion activity in the absence of integrase by using an IN-independent pathway, possibly through single-strand annealing and noncanonical homologous recombination.
Article
Cell Biology
Denghui Wei, Weixiang Zhan, Ying Gao, Liyan Huang, Run Gong, Wen Wang, Ruhua Zhang, Yuanzhong Wu, Song Gao, Tiebang Kang
Summary: RAB31 plays a dual role in exosome biogenesis by driving ILVs formation and suppressing the fusion of MVEs with lysosomes, providing a better understanding of the exosome biogenesis process.
Article
Multidisciplinary Sciences
Sophie Kneeshaw, Gonzalo Soriano, Isabel Monte, Mats Hamberg, Angel M. Zamarreno, Jose M. Garcia-Mina, Jose Manuel Franco-Zorrilla, Nobuki Kato, Minoru Ueda, Ma Fernanda Rey-Stolle, Coral Barbas, Santiago Michavila, Selena Gimenez-Ibanez, Guillermo H. Jimenez-Aleman, Roberto Solano
Summary: Jasmonate signaling regulates various processes in plants, and bryophytes contain unique C20 and C22 fatty acids. A newly identified OPDA-like molecule in the liverwort Marchantia polymorpha can activate known jasmonate responses, and LCPUFAs are shown to be a source of bioactive jasmonates essential to the immune response in M. polymorpha.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Applied
Youlu Pan, Jinghao Xu, Hongjie Zhang, Rongrong Hao, Zhengrong Shen, Wenhai Huang
Summary: A metal-free radical relay addition/cyclization reaction has been developed for the functionalization of 5,6-dihydrobenzo[4,5]imidazo[2,1-a]isoquinolines with activated alkenes, ethers, cycloalkanes, toluenes, and alcohols. The protocol offers the advantages of being metal-free, base-free, and having a shorter reaction time.
ADVANCED SYNTHESIS & CATALYSIS
(2022)
Article
Chemistry, Organic
Ekaterina E. Galenko, Aleksandra M. Puzyk, Mikhail S. Novikov, Alexander F. Khlebnikov
Summary: A method for the synthesis of 5-aryl- and 4-aryl/hetaryl/cyclopropyl/alkynyl-5-aryl-1H-pyrrole-2,3-diones by formal isomerization of isoxazole-5-carbaldehydes mediated by Mo(CO)6 in wet MeCN has been developed. The resulting 1H-pyrrole-2,3-diones serve as good precursors for substituted 1H-pyrrolo[2,3-b]quinoxalines.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Organic
Soumyadip Basu, Soumitra Rana, Pinaki Saha, Prasanta Ghosh, Chhanda Mukhopadhyay
Summary: An iron-catalyst mediated one-pot multicomponent route has been developed for the synthesis of novel 6-thioxo-hexahydroindeno[1 ',2':4,5]imidazo[1,5-a]pyridin-12(6H)-one scaffolds using ninhydrin, l-proline, and aryl isothiocyanates in ethanol. This methodology involves a number of ring-opening and ring-closing cascade steps to provide diverse substituted hexahydroindeno-imidazo[1,5-a]pyridinones in good yields. The stereochemistry of the proline ring is lost during the reaction. It is a nonhazardous, step economic, and easy operational process.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Plant Sciences
Rakesh K. Upadhyay, Jonathan Shao, Autar K. Mattoo
Summary: Identification of the polyamine biosynthetic pathway genes in duckweed S. polyrhiza shows the presence of prokaryotic as well as land plant-type ADC pathway but absence of ODC encoding genes. Differential gene expression and transcript abundance of these genes are modulated by exogenous methyl jasmonate, salinity, and acidic pH. The S. polyrhiza genome encodes for complete polyamine biosynthesis pathway and the genes are transcriptionally active in response to changing environmental conditions, suggesting an important role of polyamines in this aquatic plant.
Article
Biochemistry & Molecular Biology
Kun-Peng Jia, Jianing Mi, Shawkat Ali, Hajime Ohyanagi, Juan C. Moreno, Abdugaffor Ablazov, Aparna Balakrishna, Lamis Berqdar, Alessia Fiore, Gianfranco Diretto, Claudio Martinez, Angel R. de Lera, Takashi Gojobori, Salim Al-Babili
Summary: This study discovered a ABA biosynthetic pathway that is independent of ABA1 and identified the intermediates of this pathway as apocarotenoids. These apocarotenoids are successively converted into ABA in both Arabidopsis and rice, exerting ABA biological functions such as maintaining seed dormancy and inducing the expression of ABA-responsive genes. Furthermore, these apocarotenoids also exhibit ABA-independent regulatory activities.
Article
Multidisciplinary Sciences
Xin Gu, Christopher Nardone, Nolan Kamitaki, Aoyue Mao, Stephen J. Elledge, Michael E. Greenberg
Summary: Cells use ubiquitin to mark proteins for degradation, but the mechanism for the degradation of some proteins is unclear. This study found that midnolin promotes the degradation of nuclear proteins by binding to the proteasome, binding to substrates, and using a ubiquitin-like domain to promote substrate degradation.
Article
Chemistry, Multidisciplinary
Sharvan Kumar, Kohshi Yoshida, Yusuke Hattori, Tomohiro Higashino, Hiroshi Imahori, Shu Seki
Summary: This study reports a strategic methodology for isolation of stable negatively charged organic compounds and provides detailed descriptions of the structures and properties of two mysterious compounds. These compounds exhibit high stability under ambient conditions due to their high delocalization, and can be purified by column chromatography.
Article
Microbiology
Markus Hoffmann, Anzhalika Sidarovich, Prerna Arora, Nadine Krueger, Inga Nehlmeier, Amy Kempf, Luise Graichen, Martin S. Winkler, Daniela Niemeyer, Christine Goffinet, Christian Drosten, Sebastian Schulz, Hans-Martin Jaeck, Stefan Poehlmann
Summary: The naturally occurring mutation E484D allows SARS-CoV-2 to enter host cells independently of ACE2 and renders it insensitive to the neutralizing antibody Imdevimab, which is used for COVID-19 therapy.
Article
Agronomy
Chen Huan, Xiaohan Yang, Lufan Wang, Mariama Kebbeh, Yuxuan Wang, Binger Dai, Shuling Shen, Xiaolin Zheng, Huijuan Zhou
Summary: We screened the optimal concentration of MeJA treatment in peaches and found that MeJA treatment can alleviate chilling injury by activating alpha-linolenic acid metabolism and JA signaling pathway.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Go Suzuki, Nonawin Lucob-Agustin, Keita Kashihara, Yumi Fujii, Yoshiaki Inukai, Kenji Gomi
Summary: The study identified a loss-of-function mutant of MED25 in rice through map-based cloning and phenotypic complementation analysis, revealing its involvement in JA-mediated root development and OsMYC2-mediated leaf senescence in rice.
Article
Plant Sciences
Maria Garnica, Roberto Baigorri, Sara San Francisco, Angel M. Zamarreno, Jose M. Garcia-Mina
Summary: Studies have shown that soil and sedimentary humic acids have a close relationship with the growth of plants in calcareous soils and their ability to improve iron nutrition. This relationship may be attributed to the ability of humic acid to improve iron solubility and bioavailability, as well as its potential effects on specific mechanisms in plant roots.
FRONTIERS IN PLANT SCIENCE
(2022)
Editorial Material
Plant Sciences
Serenella Nardi, Michela Schiavon, Adele Muscolo, Diego Pizzeghello, Andrea Ertani, Luciano Pasqualoto Canellas, Jose M. Garcia-Mina
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Margarida Maria Santana, Ana Paula Rosa, Angel M. Zamarreno, Jose Maria Garcia-Mina, Abdelwahab Rai, Cristina Cruz
Summary: The effect of seed coating salt-stressed tomato with Achromobacter xylosoxidans BOA4 and/or irrigation with Enteromorpha intestinalis (EI) extract was evaluated. The addition of BOA4 and/or EI extract increased plant shoot dry weight, but decreased the root to shoot ratio. Anthocyanin content also increased with EI and BOA4 plus EI treatments. Additionally, the study found that PTN levels decreased in stressed roots or shoots with BOA4 and/or EI treatments, while cytokinins like cis-zeatin-type and iP increased.
Article
Biochemistry & Molecular Biology
Jiashu Chu, Isabel Monte, Thomas A. DeFalco, Philipp Koster, Paul Derbyshire, Frank L. H. Menke, Cyril Zipfel
Summary: In this study, the researchers discovered that MpRBOH1 and MpPBLa, single members of the RBOH and PBL families in the liverwort Marchantia polymorpha, are essential for chitin-induced ROS production. MpPBLa directly interacts with and phosphorylates MpRBOH1, and this phosphorylation is crucial for chitin-induced ROS production mediated by MpRBOH1.
Article
Biochemistry & Molecular Biology
Antonia Romero-Munar, Ricardo Aroca, Angel Maria Zamarreno, Jose Maria Garcia-Mina, Noelia Perez-Hernandez, Juan Manuel Ruiz-Lozano
Summary: Climate change is causing combined drought and high temperature stress, which significantly reduces crop production, especially for water-consuming crops like maize. This study aimed to investigate the effects of co-inoculating an AM fungus (Rhizophagus irregularis) and PGPR Bacillus megaterium (Bm) on maize plants facing combined drought and high temperature stress. The results showed that the dual AM + Bm inoculation was more effective than single inoculation, improving plant physiological responses, root hydraulic parameters, aquaporin gene expression, protein abundances, and sap hormonal content. This study demonstrates the importance of combining beneficial soil microorganisms to enhance crop productivity in the face of climate change.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Andrea Chini, Isabel Monte, Angel M. Zamarreno, Jose M. Garcia-Mina, Roberto Solano
Summary: Different plant species use different jasmonates to activate a conserved signaling pathway in land plants, with JA-Ile being the ligand for COI1/JAZ receptor in angiosperms and dn-cis-OPDA, dn-iso-OPDA, and Delta(4)-dn-iso-OPDA acting as ligands in Marchantia polymorpha. OPDA and dn-OPDA are found in all tested land plants and charophyte algae, suggesting their importance as ancestral signaling molecules. JA-Ile biosynthesis emerged in lycophytes coinciding with the evolution of JAR1 function.
Article
Plant Sciences
Eric Buehler, Elisa Fahrbach, Andreas Schaller, Nils Stuehrwohldt
Summary: Posttranslationally modified peptides, such as CLEL6, play a crucial role in regulating plant stress responses, specifically the biosynthesis of anthocyanins. CLEL6 inhibits anthocyanin biosynthesis through proteolytic processing and sulfation, and this inhibition is mediated by the RGI receptor family.
Article
Biology
Kengo Hayashi, Nobuki Kato, Khurram Bashir, Haruna Nomoto, Misuzu Nakayama, Andrea Chini, Satoshi Takahashi, Hiroaki Saito, Raku Watanabe, Yousuke Takaoka, Maho Tanaka, Atsushi J. Nagano, Motoaki Seki, Roberto Solano, Minoru Ueda
Summary: A library of all stereochemical isomers of coronatine was constructed to identify subtype-selective agonists for COI1-JAZ co-receptors in Arabidopsis thaliana and Solanum lycopersicum. The study highlights the challenge of dissecting the specific functions of receptor subtypes in gene families encoding plant hormone receptors. The identification of subtype-selective agonists is crucial for understanding the responses regulated by individual receptor subtypes.
COMMUNICATIONS BIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Marta Fuentes, German Bosch, David de Hita, Maite Olaetxea, Javier Erro, Angel Ma Zamarreno, Jose Ma Garcia-Mina
Summary: Previous studies have shown that natural heteromolecular complexes can be an alternative to synthetic chelates for correcting iron deficiency. This study investigated the interaction of these complexes with Ca2+ at alkaline pH, their stability, root uptake in cucumber, and chemical structure using molecular modeling.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Environmental Sciences
Roberto Baigorri, Gustavo Gonzalez-Gaitano, Jose Maria Garcia-Mina
Summary: Soil organic matter serves as a link between the living and mineral components of soil, providing both carbon and energy for microorganisms. The carbon cycle follows a complex evolution process, with biochemically linked structures ultimately becoming humic substances. The heat of combustion and fluorescence spectroscopy reveal the structural differentiation of different humic fractions, indicating the possibility of independent evolution into fossil fuels and coals.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Plant Sciences
Nan Wang, Zhidan Wang, Sofia Tzourtzou, Xu Wang, Xiuli Bi, Julia Leimeister, Linhao Xu, Takuya Sakamoto, Sachihiro Matsunaga, Andreas Schaller, Hua Jiang, Chang Liu
Summary: The nuclear lamina is a complex network of proteins that scaffolds the nucleus and maintains its structural integrity. In Arabidopsis, nuclear matrix constituent proteins (NMCPs) are essential for the nuclear lamina and play a role in organizing chromatin positioning. The nuclear lamina disassembles under various stresses, impacting genome organization and gene transcription.
Review
Plant Sciences
Isabel Monte
Summary: The evolution of plant hormone signaling pathways, specifically salicylic acid (SA) and Jasmonates (JAs), is closely related to the evolution of land plants. Recent studies have shown that the receptors and activation pathways of these hormones are different in non-seed plants compared to angiosperms. This review discusses the latest findings on the role of SA and JAs in non-seed plants, including studies on the bryophyte model Marchantia polymorpha.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Article
Biology
Sven Reichardt, Annick Stintzi, Andreas Schaller
Summary: Proteases play a crucial role in controlling plant growth and development by limited proteolysis, including the processing of peptide hormone precursors. Ascertainment of candidate protease as a peptide precursor-processing enzyme requires in vitro demonstration of protease-mediated precursor cleavage. In this study, we offer a protocol for the expression, purification, and characterization of tomato phytaspases as candidate proteases for phytosulfokine precursor processing, and we also demonstrate the use of synthetic oligopeptide substrates for site-specific precursor cleavage.