Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET

Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM

(2017) Claire Demeautis et al.   Scientific Reports

Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes

(2016) Emmanuel Martin et al.   JOURNAL OF CELL BIOLOGY

Optical Tools To Study the Isoform-Specific Roles of Small GTPases in Immune Cells

(2016) Veronika Miskolci et al.   JOURNAL OF IMMUNOLOGY

FRET binding antenna reports spatiotemporal dynamics of GDI–Cdc42 GTPase interactions

(2016) Louis Hodgson et al.   Nature Chemical Biology

LOVTRAP: an optogenetic system for photoinduced protein dissociation

(2016) Hui Wang et al.   NATURE METHODS

Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

(2016) Daria M. Shcherbakova et al.   Nature Communications

Natural Photoreceptors as a Source of Fluorescent Proteins, Biosensors, and Optogenetic Tools

(2015) Daria M. Shcherbakova et al.   Annual Review of Biochemistry

Near-infrared fluorescent proteins engineered from bacterial phytochromes

(2015) Daria M Shcherbakova et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Synonymous modification results in high-fidelity gene expression of repetitive protein and nucleotide sequences

(2015) Bin Wu et al.   GENES & DEVELOPMENT

A Trio–Rac1–Pak1 signalling axis drives invadopodia disassembly

(2014) Yasmin Moshfegh et al.   NATURE CELL BIOLOGY

A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging

(2014) Yao Chen et al.   Frontiers in Pharmacology

FilGAP and its close relatives: a mediator of Rho–Rac antagonism that regulates cell morphology and migration

(2013) Fumihiko Nakamura   BIOCHEMICAL JOURNAL

Rac GTPases in Human Diseases

(2013) Sung-Yun Pai et al.   DISEASE MARKERS

Near-infrared fluorescent proteins for multicolor in vivo imaging

(2013) Daria M Shcherbakova et al.   NATURE METHODS

Far-red light photoactivatable near-infrared fluorescent proteins engineered from a bacterial phytochrome

(2013) Kiryl D. Piatkevich et al.   Nature Communications

Dimerization properties of theRpBphP2 chromophore-binding domain crystallized by homologue-directed mutagenesis

(2012) Dom Bellini et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging

(2012) Daria M. Shcherbakova et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The N-Terminal DH-PH Domain of Trio Induces Cell Spreading and Migration by Regulating Lamellipodia Dynamics in a Rac1-Dependent Fashion

(2012) Jos van Rijssel et al.   PLoS One

Development of an optimized backbone of FRET biosensors for kinases and GTPases

(2011) Naoki Komatsu et al.   MOLECULAR BIOLOGY OF THE CELL

Bright and stable near-infrared fluorescent protein for in vivo imaging

(2011) Grigory S Filonov et al.   NATURE BIOTECHNOLOGY

The 'invisible hand': regulation of RHO GTPases by RHOGDIs

(2011) Rafael Garcia-Mata et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

(2011) Oksana M. Subach et al.   PLoS One

Simultaneous Visualization of Protumorigenic Src and MT1-MMP Activities with Fluorescence Resonance Energy Transfer

(2010) M. Ouyang et al.   CANCER RESEARCH

Visualization of JNK activity dynamics with a genetically encoded fluorescent biosensor

(2010) M. Fosbrink et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Coordination of Rho GTPase activities during cell protrusion

(2009) Matthias Machacek et al.   NATURE

Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome

(2009) X. Shu et al.   SCIENCE

Multiplexed FRET to Image Multiple Signaling Events in Live Cells

(2008) David M. Grant et al.   BIOPHYSICAL JOURNAL

Conversion of Red Fluorescent Protein into a Bright Blue Probe

(2008) Oksana M. Subach et al.   CHEMISTRY & BIOLOGY