期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 14, 期 6, 页码 1797-1807出版社
ELSEVIER
DOI: 10.1016/j.nano.2018.05.006
关键词
poly-adenine; spherical nucleic acid probes; tumor related mRNA; intracellular detection
资金
- National Key Research and Development Program of China [2017YFA0205302]
- National Natural Science Foundation of China [21605087, 61671250, 21475064, 21505148, U1532119, 21675167, 31470960]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R37]
- China Postdoctoral Science Foundation [BX201700123, 2018M630586]
- Natural Science Fund for Colleges and Universities in Jiangsu Province [16KJB150032]
- Scientific Research Foundation of Nanjing University of Posts and Telecommunications [NY215058]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [YX03002]
Identification of tumor-related mRNA in living cells hold great promise for early cancer diagnosis and pathological research. Herein, we present poly-adenine (polyA)-mediated fluorescent spherical nucleic acid (FSNA) probes for intracellular mRNA detection with regulable sensitivities by programmably adjusting the loading density of DNA on gold nano-interface. Gold nanoparticles (AuNPs) functionalized with polyA-tailed recognition sequences were hybridized to fluorescent reporter strands to fabricate fluorescence-quenched FSNA probes. While exposed to target gene, the reporter strands were released from FSNA through strand displacement and fluorescence was recovered. With polyA20 tail as the attaching block, the detection limit of FSNA probes was calculated to be 0.31 nM, which is -55 fold lower than that of thiolated probes without surface density regulation. Quantitative intracellular mRNA detection and imaging could be achieved with polyA-mediated FSNA probes within 2 hours, indicating their application potential in rapid and sensitive intracellular target imaging. (C) 2018 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据