Article
Biochemistry & Molecular Biology
Mohd Jahir Khan, Abrar Ahmad, Mahmood Ahmad Khan, Sahabjada Siddiqui
Summary: This study investigated the anticancer properties of zinc oxide nanoparticles (ZnO-NPs) on human epidermoid carcinoma cells and found that ZnO-NPs induce apoptosis in A431 cells through DNA degradation and generation of reactive oxygen species via p53, bax/bcl-2, and caspase pathways.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Polina Ivanova, Konrad Dziegielewski, Marcin Drozd, Sandra Skorupska, Ilona Grabowska-Jadach, Mariusz Pietrzak
Summary: Reactive oxygen species (ROS) are important in physiological processes but can pose a threat to health with increased concentrations. Different defense systems in plants, invertebrates, and vertebrates including humans combat ROS, but environmental conditions and lifestyle can interfere with enzyme activity. Therefore, finding alternative ROS scavengers is crucial, with nanozymes being a proposed solution to mimic essential enzyme activities and prevent excessive ROS activity.
Article
Polymer Science
Dmitriy A. Serov, Ilya Baimler, Dmitriy E. Burmistrov, Alexey S. Baryshev, Denis Yanykin, Maxim E. Astashev, Alexander Simakin, Sergey Gudkov
Summary: Bacterial contamination in the meat processing industry, especially on cutting boards and equipment, is a major factor causing product deterioration and loss of consumer properties. To tackle this issue, two approaches can be adopted: creating coatings with increased strength to prevent the formation of favorable conditions for bacterial growth, or developing materials with antimicrobial properties. In this study, a new coating made of polytetrafluoroethylene (PTFE) and Fe2O3 nanoparticles was developed. The coating showed bacteriostatic effects and increased the generation of reactive oxygen species (ROS). The composite material has potential applications for covering cutting boards and polymeric surfaces in the meat processing industry.
Article
Chemistry, Multidisciplinary
Zhongchao Yi, Xiaoyue Yang, Ying Liang, Fanny Chapelin, Sheng Tong
Summary: Iron oxide nanoparticles have shown great potential as a platform for ROS-dependent disease treatment. In this study, wustite nanoparticles were found to exhibit higher catalytic activity than magnetite or maghemite nanoparticles of similar size and coating. The presence of internal low-valence iron was identified as the key factor contributing to the high catalytic activity of wustite nanoparticles. Furthermore, the synthesis of ultrasmall wustite nanoparticles resulted in even higher Fenton reaction rate compared to an FDA-approved IONP. These findings provide important insights into improving the development of efficient ROS-inducing nanozymes.
Article
Nanoscience & Nanotechnology
Minh Phuong Nguyen, Yuna Kim, Dongryeol Ryu, Dukjoon Kim
Summary: This study presents an innovative chemotherapy mechanism using reactive oxygen species (ROS) and doxorubicin-loaded polyaspartamide-encapsulated superparamagnetic iron oxide nanoparticles. The nanocarriers showed high efficacy in inhibiting tumor growth and inducing cell death in cancer cells, which opens the door for future advancements in cancer-targeted chemotherapy.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Hyung Shik Kim, Sieun Lee, Dong Yun Lee
Summary: Aurozyme, a novel nanomedicine composed of gold nanoparticles and glycyrrhizin, shows promise as a therapeutic strategy for colitis. It converts harmful peroxidase-like activity to beneficial catalase-like activity, effectively scavenging reactive oxygen/reactive nitrogen species and damage-associated molecular patterns. It promotes anti-inflammatory effects, restores intestinal function, and increases beneficial probiotics, highlighting its potential for the treatment of inflammatory diseases.
Article
Plant Sciences
Indrani Manna, Saikat Sahoo, Maumita Bandyopadhyay
Summary: Researchers have found that nickel oxide nanoparticles affect the exposed roots of Allium cepa by disrupting cell membranes and being internalized within cell organelles, leading to disturbances in cellular homeostasis and survival. This external intervention causes changes in the cellular environment, reflected in crucial biochemical profiles.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Shichen Xie, Jingyao Zhu, Dicheng Yang, Yan Xu, Jun Zhu, Dannong He
Summary: The toxicity of Zinc oxide (ZnO) nanoparticles has raised concerns, with small size Zinc oxide (S-ZnO) showing stronger toxicity on human lung cells and potentially causing cell death through mitochondrial dysfunction. Additionally, a combination of dipalmitoyl phosphatidylcholine (DPPC) and ZnO nanoparticles was found to effectively reduce the toxicity on lung cells in vitro.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Temoor Ahmed, Muhammad Noman, Natasha Manzoor, Shafaqat Ali, Muhammad Rizwan, Munazza Ijaz, Khaled S. Allemailem, Abdulkarim S. BinShaya, Fahad A. Alhumaydhi, Bin Li
Summary: Engineered nanomaterials have gained attention for their beneficial applications in various fields, but their use in agriculture may lead to NPs entering the environment, impacting the food chain. Barriers to implementation include safety concerns and efficient delivery. NPs interactions with ecosystems and their toxicity are global concerns.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Environmental Sciences
Soyeon Jeon, Dong-Keun Lee, Jiyoung Jeong, Sung Ik Yang, Ji-Su Kim, Jinsik Kim, Wan-Seob Cho
Summary: This study investigated the toxicity of microplastics on different cell lines and found that microplastics showed significant toxicity to THP-1 macrophages, with toxicity correlated to the generation potential of reactive oxygen species (ROS). Weathering process reduced the ROS generation potential of microplastics, as weathered microplastics had increased affinity to bind serum protein acting as ROS scavenger.
ENVIRONMENTAL POLLUTION
(2021)
Review
Chemistry, Physical
Roberto Canaparo, Federica Foglietta, Tania Limongi, Loredana Serpe
Summary: The design, synthesis and characterization of new nanomaterials in biomedical field is a dynamic and transversal aspect of nanotechnology applications. Metal-based nanoparticles (MNPs) are widely used in biomedicine due to their physical and chemical properties. Researchers and clinicians are setting and standardizing treatments by tuning ROS production to induce cancer or microbial cell death.
Review
Chemistry, Multidisciplinary
Francesca Corsi, Greta Deidda Tarquini, Marta Urbani, Ignacio Bejarano, Enrico Traversa, Lina Ghibelli
Summary: Cerium oxide nanoparticles (CNPs) are biocompatible nanozymes with multifunctional biomimetic activities, including anti-inflammatory effects. CNPs have the ability to scavenge reactive oxygen species and boost anti-inflammatory signaling pathways, effectively combating chronic inflammation, infections, and trauma, and improving/restoring organ function. The exact mechanism of CNPs' anti-inflammatory effects is still not fully understood, but it is suggested that the pleiotropic actions of CNPs, including their phosphatase activity, may play a role.
Article
Chemistry, Physical
Patryk Szymaszek, Patrycja Sroda, Malgorzata Tyszka-Czochara, Anna Chachaj-Brekiesz, Tomasz Swiergosz, Joanna Ortyl
Summary: Here we report the synthesis of a novel fluorescent probe P182 for quantitative measurement of H2O2. This fluorometric probe displays a fluorescence turn-on response in the presence of H2O2. It offers good performances in terms of sensitivity and response time, and shows non-toxic activity for in vivo studies. This study provides research on molecular fluorescence probe for the detection of H2O2.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biochemistry & Molecular Biology
Sergey V. Gudkov, Ruibin Li, Dmitriy A. Serov, Dmitriy E. Burmistrov, Ilya V. Baimler, Alexey S. Baryshev, Alexander V. Simakin, Oleg V. Uvarov, Maxim E. Astashev, Natalia B. Nefedova, Sergey Y. Smolentsev, Andrey V. Onegov, Mikhail A. Sevostyanov, Alexey G. Kolmakov, Mikhail A. Kaplan, Andrey Drozdov, Eteri R. Tolordava, Anastasia A. Semenova, Andrey B. Lisitsyn, Vasily N. Lednev
Summary: Foodborne infections are a major global health problem with high prevalence and severe complications. Bacterial contamination during meat processing can cause foodborne infections, and antibacterial coatings can be used to prevent it. In this study, a composite coating based on fluoroplast and Ag2O NPs was developed for the first time. The coating showed excellent antibacterial properties against both Gram-positive and Gram-negative bacteria, without affecting the viability of eukaryotic cells. This coating could be used to protect cutting boards from bacterial contamination in the meat processing industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuqing He, Qiang Gao, Chenglin Lv, Lijun Liu
Summary: The study describes the design and development of functionalized acrylic polymeric nanoparticles with Spiropyrans and imidazole moieties via superficial polymerizations, and the immobilization and reduction of Au3+ ions on their surface to obtain photoresponsive gold-decorated polymer nanoparticles(Au-NPs). The Au-NPs surface adapted with biotin showed enhanced cellular uptake and improved targeting efficacy towards C6 glioma cells, with the potential for targeted cancer therapy.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hui Lu, Jinsong Fan, Guangliu Ran, Jiang Li, Lihua Wang, Ying Zhu, Jianlei Shen, Wenkai Zhang, Jing Chen, Chunhai Fan
Summary: This study reports the functionalization of bimetallic nanoclusters Au8Ag8(C(sic)CR)(16) with triarylborane, which enhances the Lewis acidity. The structure of the nanocluster with 16 boron receptor sites is confirmed, showing increased quenching efficiency towards fluoride. The quenching mechanism of Au8Ag8 to fluoride is demonstrated via enhanced non-radiation energy dissipation.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Xingjie Hu, Yan Huang, Hong Zheng, Jiahui Liu, Mengmeng Liu, Mo Xie, Chunhai Fan, Nan Chen
Summary: Framework nucleic acids (FNAs) are a new type of DNA-based nanomaterials with potential applications in biosensing, bioimaging, and molecular delivery. Hierarchical DNA nanostructures composed of multiple FNAs increase drug delivery capacity and multifunctional modification. However, there are limited studies on the behavior and regulation of hierarchical FNAs in living cells, hindering their further applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Cuiping Mao, Shuchen Wang, Jiankai Li, Zhuowei Feng, Tong Zhang, Rui Wang, Chunhai Fan, Xingyu Jiang
Summary: DNA, as a storage medium, offers high information density but requires specialized equipment and controlled environments. We introduce a DNA microlibrary encapsulated in metal-organic frameworks (MOFs) within 10 minutes and extracted in 5 minutes using a microfluidic chip for automated and integrated DNA-based data storage. The DNA microlibrary@MOFs enhances the stability of data-encoded DNA against harsh environments and can be read out perfectly after accelerated aging, equivalent to 10-year storage under specific conditions.
Review
Chemistry, Multidisciplinary
Fei Ding, Shuangye Zhang, Qian Chen, Hao Feng, Zhilei Ge, Xiaolei Zuo, Chunhai Fan, Qian Li, Qiang Xia
Summary: Programmable nucleic acid nanotechnology allows the customization of synthetic nucleic acid nanodevices with unprecedented precision, showing potential for precise immunoengineering.
Article
Chemistry, Multidisciplinary
Yuxin Xing, Lin Li, Yuhua Chen, Lu Wang, Shuqi Tang, Xiyue Xie, Shuai Wang, Jixi Huang, Kaiyong Cai, Jixi Zhang
Summary: A flower-like nanozyme with highly permeable leaflets has been developed to increase the efficacy of antioxidants and antiapoptotic defense systems in therapy-resistant tumors. The nanozyme generates peroxidase/oxidase-like activities with high catalytic efficiency, leading to a robust ROS storm and rapid accumulation of oxidative damage in drug-resistant cancer cells. By combining with a redox homeostasis disrupter, a significantly high antitumor efficiency was achieved in vivo. This work provides a promising approach to ROS generators for the treatment of therapy-resistant tumors.
Article
Chemistry, Multidisciplinary
Haipei Zhao, Xuehao Xiu, Mingqiang Li, Shaobo Dai, Mingyang Gou, Leyang Tao, Xiaolei Zuo, Chunhai Fan, Zhongqun Tian, Ping Song
Summary: Dynamic interactions of enzymes are important in regulating cellular metabolism. A super DNA-enzymes molecule was constructed for controlling and detecting metabolism. The programmable SDEM shows improved detection limit and reaction rate compared to free enzymes. The SDEM can be assembled and disassembled using a hairpin structure and a displacement DNA strand for multiple cycles. An entropically driven catalytic assembly enables quick switching between different SDEMs for detecting different metabolisms on demand.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Fengyun Shen, Zijian Xiong, Yumin Wu, Rui Peng, Yue Wang, Lele Sun, Chunhai Fan, Zhuang Liu
Summary: Researchers constructed framework nucleic acids (FNAs) with regulated rigidity and size to organize epitopes and investigated their influence on peptide vaccine efficiency. They found that assembling epitopes on rigid tetrahedral FNAs could enhance their immunogenicity. By integrating epitopes from SARS-CoV-2 on preferred tFNAs, a COVID-19 peptide vaccine was developed, inducing high antibody titers and increasing memory B and T cell ratio in mice. This research provides a new idea for developing efficient peptide vaccines.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Xiangyuan Ouyang, Yongli Wu, Linjie Guo, Le Li, Mo Zhou, Xinyi Li, Ting Liu, Yawen Ding, Huaiyu Bu, Gang Xie, Jianlei Shen, Chunhai Fan, Lihua Wang
Summary: In this study, the ordered self-assembly of CuNCs using DNA nanoribbon as the template (DNR/CuNCs) was found to improve the electrochemiluminescence (ECL) properties of CuNCs compared with individual CuNCs. The DNR/CuNCs showed a high ECL yield of 46.8% in K2S2O8, which was approximately 68 times higher than that of individual CuNCs. This strategy was also successfully applied to other ECL emitters, such as gold nanoclusters and the Ru(bpy)(3)(2+)/TPrA system. Furthermore, a DNR/CuNC-based ECL biosensor with higher sensitivity was constructed for dopamine determination, showing the potential of DNR/CuNCs as a new type of superior luminophore candidate in ECL bioanalysis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Mo Xie, Weina Fang, Zhibei Qu, Yang Hu, Yichi Zhang, Jie Chao, Jiye Shi, Lihua Wang, Lianhui Wang, Yang Tian, Chunhai Fan, Huajie Liu
Summary: The authors have developed a DNA origami-based metallization reaction concept to synthesize highly entropic nanoalloys with controlled morphology, composition and uniformity. These multimetallic nanopatterns hold great potential in nanoelectronics, nanophotonics and catalysis.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yinan Zhang, Xiaoyao Yin, Chengjun Cui, Kun He, Fei Wang, Jie Chao, Tao Li, Xiaolei Zuo, Ailing Li, Lihua Wang, Na Wang, Xiaochen Bo, Chunhai Fan
Summary: In this study, a model based on DNA origami frameworks (DOFs) is designed to solve the prime factorization problem. The model uses DX tiles to assemble prime and composite integers, and the result is visualized using atomic force microscopy. This DNA-based parallel computing strategy has the potential to solve complex mathematical puzzles like prime factoring.
Article
Chemistry, Multidisciplinary
Qinqin Hu, Zongxuan Tong, Ayimukedisi Yalikong, Li-Ping Ge, Qiang Shi, Xinyu Du, Pu Wang, Xi-Yu Liu, Wuqiang Zhan, Xia Gao, Di Sun, Tong Fu, Dan Ye, Chunhai Fan, Jie Liu, Yun-Shi Zhong, Yi-Zhou Jiang, Hongzhou Gu
Summary: Biomarker discovery is crucial for understanding and diagnosing malignant diseases, but the lack of sensitive methods to identify rare disease-specific molecules remains a challenge. This study presents MORAC, a method that utilizes DNAzymes to identify candidate biomarkers with low abundance. By exploiting the catalysis and affinity of DNAzymes, previously unknown biomarkers with clear clinical value can be identified.
Article
Multidisciplinary Sciences
Yun Zhu, Xiewei Xiong, Mengyao Cao, Li Li, Chunhai Fan, Hao Pei
Summary: Freeze-thaw cycling is a simple and powerful method for high-speed DNA computing, achieving a 20-fold speed enhancement in strand displacement reactions. This acceleration is due to the use of eutectic ice phase as a medium, temporarily increasing the effective local concentration of molecules during each cycle. By leveraging this phenomenon, freeze-thaw cycling can achieve up to a remarkable 120-fold enhancement in reaction rates across various circuit sizes.
Review
Chemistry, Multidisciplinary
Longjiang Ding, Xiaoliang Chen, Wenhe Ma, Jiang Li, Xiaoguo Liu, Chunhai Fan, Guangbao Yao
Summary: How far we can push chemical self-assembly is one of the most important scientific questions of the century. Colloidal self-assembly is a bottom-up technique for the rational design of functional materials with desirable collective properties. The regioselective control over the formation of DNA bonds on the particle surface has enabled unprecedented complexity in colloidal self-assembly, thanks to the advances in surface chemistry and structural DNA nanotechnology. This review summarizes the recent advances in DNA-mediated regioselective surface encoding of colloids, with a focus on its introduction and its crucial role in the self-assembly of colloidal structures.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Longyi Zhu, Yiting Shen, Shengyuan Deng, Ying Wan, Jun Luo, Yan Su, Mingxu You, Chunhai Fan, Kewei Ren
Summary: Researchers have developed a synthetic DNA binder that can induce mitochondrial aggregation and fusion in living cells. This DNA binder has shown potential for repairing ROS-stressed neuron cells and can be further customized to achieve stimuli-triggered mitochondrial aggregation and fusion. The researchers believe that this new DNA regulator system can be a powerful tool for subcellular manipulation and precision therapy.
Article
Chemistry, Multidisciplinary
Yuxin Xing, Lin Li, Yuhua Chen, Lu Wang, Shuqi Tang, Xiyue Xie, Shuai Wang, Jixi Huang, Kaiyong Cai, Jixi Zhang
Summary: A flower-like nanozyme with highly permeable leaflets containing catalytic metal sites was developed to overcome the limitations of reactive oxygen species (ROS) generators in the sensitization and treatment of therapy-resistant tumors. The highly porous nanozyme with cobalt single-atom/cluster sites exhibited significantly higher catalytic efficiency compared to conventional nano-/biozymes, leading to rapid accumulation of oxidative damage and failure of antioxidant and antiapoptotic defense synchronization in drug-resistant cancer cells. The synergistic effect of a co-delivered redox homeostasis disrupter further enhanced the antitumor efficacy of the nanozyme.
