期刊
NANO LETTERS
卷 18, 期 3, 页码 1962-1971出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.7b05354
关键词
DNA nanotechnology; single-molecule FRET; optical voltage measurements; nanocapillary; coarse-grained simulations
类别
资金
- Swiss National Science Foundation (SNF)
- Deutsche Forschungsgesellschaft DFG [TI 329/10-1]
- DFG [TI 329/10-1]
- nanosystems initiative Munich (NIM)
- center for integrated protein science Munich (CIPSM)
- EPSRC [EP/K016636/1]
- ERC [647144]
- National Science Foundation [DMR-1507985, PHY-1430124]
- National Institutes of Health [P41-GM104601]
- XSEDE Allocation Grant [MCA05S028]
- Engineering and Physical Sciences Research Council [EP/K016636/1] Funding Source: researchfish
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1507985] Funding Source: National Science Foundation
- Division Of Physics
- Direct For Mathematical & Physical Scien [1430124] Funding Source: National Science Foundation
- EPSRC [EP/K016636/1] Funding Source: UKRI
We explore the potential of DNA nanotechnology for developing novel optical voltage sensing nanodevices that convert a local change of electric potential into optical signals. As a proof-of-concept of the sensing mechanism, we assembled voltage responsive DNA origami structures labeled with a single pair of FRET dyes. The DNA structures were reversibly immobilized on a nanocapillary tip and underwent controlled structural changes upon application of an electric field. The applied field was monitored through a change in FRET efficiency. By exchanging the position of a single dye, we could tune the voltage sensitivity of our DNA origami structure, demonstrating the flexibility and versatility of our approach. The experimental studies were complemented by coarse-grained simulations that characterized voltage-dependent elastic deformation of the DNA nanostructures and the associated change in the distance between the FRET pair. Our work opens a novel pathway for determining the mechanical properties of DNA origami structures and highlights potential applications of dynamic DNA nanostructures as voltage sensors.
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