期刊
MOLECULES
卷 23, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/molecules23061278
关键词
electrophoresis; calves; clinoptilolite; gamma-globulin
资金
- Interdisciplinary Research Platform and Research Laboratory Center Horia Cernescu - infrastructure project POSCCE SMIS [2669]
The objective of our study was to determine the effects of clinoptilolite supplemented in colostrum on the blood serum protein electrophoretic pattern of new-born calves. Methods: Romanian Black and White new-born calves involved in the study were divided into 3 groups: the control group (C) that received colostrum without clinoptilolite, and experimental groups I (E1) and II (E2) that received colostrum supplemented with 0.5% and 2% clinoptilolite, respectively. The concentration of total protein and protein fractions (albumin, alpha 1-globulin, alpha 2-globulin, beta-globulin and gamma-globulin) were analyzed by electrophoresis on cellulose acetate. Results: At hour 30 after birth, concentrations of gamma-globulins, beta-globulin and total protein in E1 group of calves were higher than in control group by 42.11% (p < 0.05), 28.48% (p > 0.05) and 18.52% (p > 0.05), respectively, and were higher, but not significantly, in group E2 compared to the control group. This was in accordance with a significant lower albumin/globulin ratio in groups E1 and E2 (29.35%, p < 0.05 and 35.87%, p < 0.05, respectively) than in control group at 30 h postpartum, which indicates an obvious increase of the globulins fraction in experimental groups. The conclusion: Clinoptilolite was effective in improving passive transfer in new-born calves, but it was more effective if added in colostrum with a dose of 0.5% than with a dose of 2%.
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