Article
Microbiology
Osakina Aron, Min Wang, Lianyu Lin, Wajjiha Batool, Birong Lin, Ammarah Shabbir, Zonghua Wang, Wei Tang
Summary: The study revealed that MoGLN2 plays a crucial role in the development, cell wall integrity, oxidative stress tolerance, and pathogenicity of plant fungal pathogens, while MoGLN1 and MoGLN3 are not involved in these processes.
Article
Plant Sciences
Deng Chen, Muhammad Kamran, Shen Chen, Junjie Xing, Zhiguang Qu, Caiyun Liu, Zhiyong Ren, Xuan Cai, Xiao-Lin Chen, Jingbo Xu
Summary: Cell wall polysaccharides are crucial for fungal development, virulence, and resistance. In rice blast fungus, two nucleotide sugar transporters (NSTs), NST1 and NST2, were identified. NSTs were found to be involved in transporting specific sugars and played important roles in cell wall integrity, asexual development, and virulence. Deletion of NSTs resulted in decreased soluble saccharides abundance, defect in sugar utilization, and activation of host defense response.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Microbiology
Bin Qian, Xinyu Liu, Ziyuan Ye, Qikun Zhou, Peng Liu, Ziyi Yin, Wenhao Wang, Xiaobo Zheng, Haifeng Zhang, Zhengguang Zhang
Summary: MoTip41 plays a vital role in the development and pathogenicity of the rice blast fungus, meditating crosstalk between the TOR and CWI signaling pathways. Its deletion leads to various abnormalities and increased sensitivity to rapamycin.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Ning Liu, Linlu Qi, Manna Huang, Deng Chen, Changfa Yin, Yiying Zhang, Xingbin Wang, Guixin Yuan, Rui-Jin Wang, Jun Yang, You-Liang Peng, Xunli Lu
Summary: This study reveals the importance of Alg3, an alpha-1,3-mannosyltransferase, in the secretion and localization of proteins in Magnaporthe oryzae. The researchers identified 51 proteins that require Alg3 for proper secretion, which are involved in metabolic processes, interspecies interactions, cell wall organization, and response to chemicals. The study also demonstrates the essential role of N-glycosylation in the function and secretion of specific proteins, as well as the impact of Alg3 mutation on fungal virulence and cell wall integrity.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Microbiology
Ai-Ai Zhou, Rong-Yu Li, Fei-Xu Mo, Yi Ding, Ruo-Tong Li, Xue Guo, Ke Hu, Ming Li
Summary: The study found that citronellal has a strong inhibitory effect on Magnaporthe oryzae, a rice blast fungus. It can affect the synthesis of chitin in the fungus and damage its cell wall, thereby inhibiting the growth of the fungus and effectively protecting rice from rice blast.
Article
Plant Sciences
Shiyi Liu, Xinli Gong, Ji Ma, Shuaishuai Wang, Min Guo
Summary: Asexual spore is essential for rice blast disease, and its differentiation from conidiophore is regulated by cell cycle. The role of Mih1 homologue in Magnaporthe oryzae remains unknown. This study characterized the function of MoMih1 in M. oryzae and found that it is involved in cell cycle regulation and virulence. MoMih1 interacts with MoCdc28 and its loss leads to delayed nucleus division and impaired fungal growth and conidiation. MoMih1 mutants also show defects in penetration, biotrophic growth, and scavenging of reactive oxygen species. Additionally, MoMih1 is implicated in cellular processes such as localization of retromer protein and cell wall integrity.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Lin Li, Xue-Ming Zhu, Jia-Qi Wu, Na Cao, Jian-Dong Bao, Xiao-Hong Liu, Fu-Cheng Lin
Summary: The study identified the unique LAMMER kinase MoKns1 in the rice pathogen Magnaporthe oryzae and revealed its crucial roles in regulating autophagy and DNA damage response pathways, affecting virulence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xiaomin Chen, Su Pan, Huimin Bai, Jiaxin Fan, Wajjiha Batool, Ammarah Shabbir, Yijuan Han, Huakun Zheng, Guodong Lu, Lili Lin, Wei Tang, Zonghua Wang
Summary: Rice blast caused by Magnaporthe oryzae is a highly destructive disease that threatens food security worldwide. Researchers have discovered a nonclassically secreted nuclear targeting effector in M. oryzae (MoNte1) that is important for fungal growth and pathogenicity. This effector is able to enter plant nuclei and induce cell death, providing new insights into the interactions between rice and M. oryzae.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xingyu Liu, Tingting Bao, Li Zheng, Veronica T. Kgosi, Xinyu Liu, Hongxia Liu
Summary: The study demonstrated that proteins secreted by Bacillus licheniformis BL06 have a strong antimicrobial effect on the rice blast pathogen, Magnaporthe oryzae. This effect is achieved by inhibiting conidial germination, downregulating key chitin synthesis genes, and reducing chitin content in the pathogen. The results suggest that BL06-SP may provide a new pathway for controlling rice blast by targeting specific chitin synthesis genes.
BIOLOGICAL CONTROL
(2021)
Article
Immunology
Lina Yang, Xiaohong Liu, Jie Wang, Lianwei Li, Wanzhen Feng, Zhaolin Ji
Summary: Vitamin B6 plays an important role in the early infectious stages of M. oryzae, and its deficiency affects the growth, invasiveness, and pathogenicity of the fungus. The lack of vitamin B6 results in abnormal turgor pressure in cells and spores, which affects the growth of hyphae and the invasiveness of M. oryzae. Vitamin B6 is synthesized and transported into the appressorium, where it regulates the internal pressure. Additionally, MoPdx1 may participate in multiple pathways, particularly in ribosome and biosynthesis of certain substances.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Lianwei Li, Yanru Li, Kailun Lu, Rangrang Chen, Jihong Jiang
Summary: Bacillus subtilis strain KLBMPGC81 and its supernatant effectively suppress rice blast disease by affecting the growth, appressorium formation, and host infection of Magnaporthe oryzae.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Osakina Aron, Frankine Jagero Otieno, Ibrahim Tijjani, Zifeng Yang, Huxiao Xu, Shuning Weng, Jiayuan Guo, Songmao Lu, Zonghua Wang, Wei Tang
Summary: This study identified and characterized the functions of MoAde4, an orthologue of yeast Ade4 in Magnaporthe oryzae. The results demonstrated that MoAde4 plays a crucial role in fungal development and pathogenicity, suggesting it as a suitable target for antifungal development against recalcitrant plant fungal pathogens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Kailun Lu, Rangrang Chen, Yi Yang, Hui Xu, Jihong Jiang, Lianwei Li
Summary: The fungal cell wall plays a crucial role in regulating cellular stability and protection. The cell wall-integrity pathway is a primary signaling cascade responsible for maintaining cell wall structure and function. This pathway is associated with pathogenicity and collaborates with other signaling pathways to regulate cellular morphogenesis and secondary metabolism.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Environmental Sciences
Wanqiu Chen, Yingrong Zhang, Zhenhua Shi
Summary: This study found that exposure to 50 Hz, 3 mT ELF-EMF can enhance the pathogenicity, growth rate, and conidia generation of Magnaporthe oryzae. The exposed M. oryzae also showed increased tolerance to cell wall-perturbing agents and increased expression of cell wall integrity-related genes. These findings present new challenges for understanding the effects of ELF-EMF exposure on farmland ecology, especially on agricultural diseases.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Zhirong Peng, Yuefeng Fu, Fan Wang, Qiqi Liu, Yi Li, Zhengbing Zhang, Li Yin, Xiao-Lin Chen, Jingbo Xu, Huafeng Deng, Junjie Xing
Summary: This study analyzed the population structure and variation tendency of 462 Magnaporthe oryzae isolates from different areas of Hunan Province in 2016 and 2018. The results showed that from 2016 to 2018, the concentration of fungal races increased and the diversity decreased. The dominant-population ZB group accounted for a high percentage in both years.
Article
Microbiology
Bin Qian, Xinyu Liu, Jia Jia, Yongchao Cai, Chen Chen, Haifeng Zhang, Xiaobo Zheng, Ping Wang, Zhengguang Zhang
ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Plant Sciences
Lina Yang, Yanyan Ru, Xingjia Cai, Ziyi Yin, Xinyu Liu, Yuhan Xiao, Haifeng Zhang, Xiaobo Zheng, Ping Wang, Zhengguang Zhang
MOLECULAR PLANT PATHOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Lianwei Li, Shengpei Zhang, Xinyu Liu, Rui Yu, Xinrui Li, Muxing Liu, Haifeng Zhang, Xiaobo Zheng, Ping Wang, Zhengguang Zhang
MOLECULAR PLANT-MICROBE INTERACTIONS
(2019)
Article
Microbiology
Xiao Li, Kaili Zhong, Ziyi Yin, Jiexiong Hu, Wenhao Wang, Lianwei Li, Haifeng Zhang, Xiaobo Zheng, Ping Wang, Zhengguang Zhang
Article
Microbiology
Yuhan Xiao, Luping Liu, Ting Zhang, Ruiwen Zhou, Yuan Ren, Xinrui Li, Haidong Shu, Wenwu Ye, Xiaobo Zheng, Zhengguang Zhang, Haifeng Zhang
Summary: The study demonstrates the essential role of the transcription factor MoMsn2 in maintaining mitochondrial morphology and regulating mitochondria-related genes. MoMsn2 targets MoAUH1 to control mitochondrial morphology and mitophagy, which are crucial for the pathogen's infectious growth.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Bin Qian, Xinyu Liu, Ziyuan Ye, Qikun Zhou, Peng Liu, Ziyi Yin, Wenhao Wang, Xiaobo Zheng, Haifeng Zhang, Zhengguang Zhang
Summary: MoTip41 plays a vital role in the development and pathogenicity of the rice blast fungus, meditating crosstalk between the TOR and CWI signaling pathways. Its deletion leads to various abnormalities and increased sensitivity to rapamycin.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Plant Sciences
Muxing Liu, Jiexiong Hu, Ao Zhang, Ying Dai, Weizhong Chen, Yanglan He, Haifeng Zhang, Xiaobo Zheng, Zhengguang Zhang
Summary: This study revealed a novel role of the COPII uncoating factor MoSwa2 in vesicular transport, showing its critical importance in suppressing host immunity and pathogenicity in M. oryzae. MoSwa2 deficiency resulted in impaired secretion of extracellular proteins, affecting the growth of invasive hyphae and the inhibition of host immunity. Additionally, the study identified the extracellular protein MoSef1 as an apoplastic virulent factor that inhibits host immune response.
Review
Biochemistry & Molecular Biology
Xinyu Liu, Zhengguang Zhang
Summary: During the interaction between Magnaporthe oryzae and rice, reactive oxygen species (ROS) play important roles in signaling reactions. Rice generates ROS to restrict the spreading of invasive hyphae, while the pathogen responds and suppresses ROS accumulation through the secretion of effector molecules.
Article
Plant Sciences
Bin Qian, Xiaotong Su, Ziyuan Ye, Xinyu Liu, Muxing Liu, Danyu Shen, Han Chen, Haifeng Zhang, Ping Wang, Zhengguang Zhang
Summary: During interactions between plant-pathogenic fungi and host plants, the COPII cargo receptor MoErv29 serves as a target of the transcription factor MoHac1, crucial for efficient delivery of secreted proteins. Deletion of MoERV29 in Magnaporthe oryzae significantly impairs vegetative growth, conidiation, and biotrophic invasion in susceptible rice hosts. By recognizing and binding amino-terminal tripeptide motifs, MoErv29 is essential for the secretion of various proteins, including laccases and effectors important for host recognition and disease resistance.
Article
Plant Sciences
Yongchao Cai, Xinyu Liu, Lingbo Shen, Nian Wang, Yangjie He, Haifeng Zhang, Ping Wang, Zhengguang Zhang
Summary: This study identified two protein phosphatases MoPtc1 and MoPtc2 as negative regulators of the cell wall integrity (CWI) pathway in the rice blast fungus Magnaporthe oryzae, crucial for maintaining the stability of the CWI signaling pathway and plant pathogenicity.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Ziqian Guo, Xinyu Liu, Nian Wang, Pengcheng Mo, Ju Shen, Muxing Liu, Haifeng Zhang, Ping Wang, Zhengguang Zhang
Summary: This study revealed the role of ergosterol in the infection of the rice blast fungus Magnaporthe oryzae. Ergosterol-enriched lipid rafts were found to be crucial for the interaction among various SNARE proteins that are required for the development and pathogenicity of M. oryzae.
Article
Plant Sciences
Nan Guo, Hongye Qu, Yue Zhi, Yuyi Zhang, Shujing Cheng, Jinfang Chu, Zhengguang Zhang, Guohua Xu
Summary: Plant amino acid transporters play crucial roles in regulating long-distance nitrogen transport, amino acid allocation, and defense against invading pathogens. However, the specific function of amino acid transporters in plant defense against pathogen infection remains unknown. In this study, researchers discovered that the rice amino acid transporter gene OsLHT1 is expressed in leaves and is up-regulated in response to maturation, nitrogen starvation, and infection by the rice blast fungus Magnaporthe oryzae. Knockout of OsLHT1 resulted in premature leaf senescence and sustained rusty red spots on mature leaves, regardless of nitrogen supply. Disruption of OsLHT1 also affected the transport and metabolism of amino acids, as well as the biosynthesis of flavones and flavonoids, leading to increased expression of defense genes, production of jasmonic acid and salicylic acid, and accumulation of reactive oxygen species. Importantly, inactivation of OsLHT1 significantly inhibited leaf invasion by M. oryzae. These findings establish OsLHT1 as a key regulator in the crosstalk between leaf appearance and resistance to rice blast fungus by mediating leaf amino acid homeostasis, ROS accumulation, and JA and SA production.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Genetics & Heredity
Jiayun Xu, Xinyu Liu, Wei Zhang, Wanzhen Feng, Muxing Liu, Leiyun Yang, Zhixiang Yang, Haifeng Zhang, Zhengguang Zhang, Ping Wang
Summary: During its pathogenic interaction with rice, the rice blast fungus Magnaporthe oryzae forms specialized structures called appressoria to initiate infection. In this study, it was found that phosphorylation of the regulator of G-protein signaling (RGS)-like protein MoRgs7 by the protein kinase MoSep1 regulates its function. MoRgs7 phosphorylation determines its interaction with the actin-binding protein MoCrn1, which also modulates its internalization. Importantly, the internalization of MoRgs7 is critical for its function in cAMP signaling for appressorium formation and fungal pathogenicity.
Article
Microbiology
Bin Qian, Xiaotong Su, Ziyuan Ye, Xinyu Liu, Muxing Liu, Haifeng Zhang, Ping Wang, Zhengguang Zhang
Summary: This study reveals the important role of COPII protein MoErv14 in the appressorium formation and pathogenicity of Magnaporthe oryzae. MoErv14 mediates the transport of multiple membrane receptors to promote appressorium formation and virulence.