期刊
MOLECULAR IMAGING AND BIOLOGY
卷 20, 期 6, 页码 930-939出版社
SPRINGER
DOI: 10.1007/s11307-017-1158-7
关键词
Matrix metalloproteinase; Image-guided surgery; Glioma; Fluorescence molecular tomography
资金
- National Natural Science Foundation of China [81227901, 81470083, 81527805, 61231004]
- Research and Development Program of China (973) [2014CB748600, 2015CB755500]
- Strategic Priority Research Program from the Chinese Academy of Sciences [XDB02060010]
- International Innovation Team of CAS [20140491524]
- Beijing Municipal Science & Technology Commission [Z161100002616022]
- Beijing Natural Science Foundation [Z16110200010000]
PurposeMalignant gliomas are major causes of cancer-related mortality and morbidity. Traditional surgery usually leads to incomplete resection of gliomas resulting in the high incidence of tumor recurrence. Advanced medical imaging technology, such as fluorescence imaging-guided surgery, combined with tumor-specific imaging probes allows the identification of tumor margins and improved surgery. However, there are two pressing issues that need to be addressed: first, few fluorescence imaging probes can specifically target gliomas; second, fluorescence molecular imaging (FMI) cannot get the in-depth information of deep-seated gliomas; both of which affect the complete removal of the gliomas.ProceduresIn this study, the biodistribution of smart matrix metalloproteinase (MMP) targeting near-infrared (NIR) fluorescent probe MMPSense 750 FAST (MMP-750) was examined in both U87MG-GFP-fLuc glioma xenograft and orthotopic mouse models using FMI. Then, CT and FMI images of orthotopic gliomas were acquired for the reconstruction of fluorescence molecular tomography (FMT) using a randomly enhanced adaptive subspace pursuit (REASP) algorithm. Furthermore, the resection of orthotopic glioma was performed using the fluorescence surgical navigation system after the injection of the MMP-750 probe. After surgery, bioluminescence imaging (BLI) and hematoxylin and eosin staining were carried out to confirm the precision resection of the tumor.ResultsFMI results showed that the MMP-750 probe can specifically target U87MG glioma in vivo. FMT presented the spatial information of the orthotopic glioma using the REASP reconstruction algorithm. Furthermore, MMP-750 could effectively delineate the tumor margin during glioma surgery leading to a complete resection of the tumors.ConclusionsThe smart MMP-750 specifically targets the glioma and FMT of MMP-750 provides 3D information for the spatial localization of the glioma. MMP-750 can work as an ideal fluorescence probe for guiding the intraoperative surgical resection of the glioma, possessing clinical translation.
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