Article
Biochemistry & Molecular Biology
Xiaofan Jin, Geoff Fudenberg, Katherine S. Pollard
Summary: Active, spatially accessible genomic regions during meiotic prophase are associated with DSB-favored loci, which further adopt a transient locally active configuration in early prophase. Conversely, crossover formation is depleted among DSBs in spatially accessible regions during meiotic prophase, particularly within gene bodies. Active chromatin regions have smaller average loop sizes in mammalian meiosis, indicating differences in chromatin architecture along chromosomal axes are associated with variable recombination activity.
Review
Cell Biology
Vikash Kumar Yadav, Corentin Claeys Bouuaert
Summary: The article outlines the latest advances in understanding the mechanism of meiotic DSB formation, emphasizing the tight regulation of DSBs and the significance of recombination mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Florencia Pratto, Kevin Brick, Gang Cheng, Kwan-Wood Gabriel Lam, Jeffrey M. Cloutier, Daisy Dahiya, Stephen R. Wellard, Philip W. Jordan, R. Daniel Camerini-Otero
Summary: This passage discusses the distribution of genetic recombination in the genome, the role of the PRDM9 protein, and the impact of meiotic replication on recombination. Research findings show that meiotic DNA replication is distinct from replication in somatic cells, and this has important implications for understanding genetic recombination and the recombination potential of individual chromosomes.
Article
Plant Sciences
Yan Wang, Shu-Yue Li, Ya-Zhong Wang, Yan He
Summary: By integrating genetic analysis, immunostaining technology, and protein-protein interaction studies, the putative factors linking DSB formation to chromosome axis were explored in maize meiosis. It was found that ZmASY1 and ZmPRD3 may work as a key module linking DSB sites to chromosome axes during DSB formation in maize.
Article
Cell Biology
Yaping Huang, Lin Li, Geng An, Xinyan Yang, Manman Cui, Xiuling Song, Jing Lin, Xiaoling Zhang, Zhaokai Yao, Cong Wan, Cai Zhou, Jiexiang Zhao, Ke Song, Shaofang Ren, Xinyu Xia, Xin Fu, Yu Lan, Xuesong Hu, Wen Wang, Mei Wang, Yi Zheng, Kai Miao, Xiaochun Bai, Andrew P. Hutchins, Gang Chang, Shuai Gao, Xiao-Yang Zhao
Summary: Huang, Li, An, Yang, Cui et al. perform a single-cell multi-omics analysis of human spermatogenesis and identify a DNA demethylation event upon meiosis initiation, which is associated with meiotic recombination.
NATURE CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Byeong Seong Ki, Sung Han Shim, Chanhyeok Park, Hyunjin Yoo, Hyeonwoo La, Ok-Hee Lee, Youngjoo Kwon, David G. Skalnik, Yuki Okada, Ho-Geun Yoon, Jin-Hoi Kim, Kwonho Hong, Youngsok Choi
Summary: A protein called Cfp1 has been found to play an important role in the development of sperm cells in mice. Its absence interferes with the process of meiosis, which leads to abnormal sperm formation. Cfp1 controls gene expression, including genes related to meiosis and homologous recombination.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Genetics & Heredity
Fabien Dutreux, Abhishek Dutta, Emilien Peltier, Sabrina Bibi-Triki, Anne Friedrich, Bertrand Llorente, Joseph Schacherer
Summary: In this study, the non-model budding yeast Lachancea waltii was used to investigate meiotic recombination. It was found that L. waltii frequently lacks crossover, suggesting the existence of an alternative mechanism for gamete formation. The interference between meiotic crossovers in L. waltii was reduced compared to S. cerevisiae, and crossover hotspots were not conserved between Lachancea and Saccharomyces clades. This study demonstrates that meiotic recombination can evolve at a limited evolutionary scale within budding yeasts.
Article
Plant Sciences
Yazhong Wang, Yan Wang, Jie Zang, Huabang Chen, Yan He
Summary: The function of PRD1 in regulating meiotic DSB formation is conserved in maize, but its role in bipolar spindle assembly shows divergence among different plant species. ZmPRD1 is essential for DSB formation and homologous recombination in maize meiosis, but the function of PRD1 in bipolar spindle assembly during rice meiosis is not conserved in maize.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Hamida Laroussi, Ariadna B. Juarez-Martinez, Aline Le Roy, Elisabetta Boeri Erba, Frank Gabel, Bernard de Massy, Jan Kadlec
Summary: Meiotic recombination in mice is initiated by DNA double-strand breaks (DSBs), catalyzed by the TOPOVIL complex. This complex's activity is regulated by meiotic factors such as REC114, MEI4, and IHO1, but the mechanism is not well understood. This study reveals the molecular details of these factors and their interactions, including REC114 forming homodimers, associating with MEI4 as a heterotrimer, and IHO1 forming tetramers. The study also shows that IHO1 directly interacts with REC114 and shares a binding surface with TOPOVIBL and another meiotic factor ANKRD31. These findings suggest the existence of a ternary IHO1-REC114-MEI4 complex and highlight the potential regulatory role of REC114 in mediating interactions with multiple partners.
Article
Multidisciplinary Sciences
Hui Ma, Tao Li, Xuefeng Xie, Long Jiang, Jingwei Ye, Chenjia Gong, Hanwei Jiang, Suixing Fan, Huan Zhang, Baolu Shi, Beibei Zhang, Xiaohua Jiang, Yang Li, Jianteng Zhou, Jianze Xu, Xingxia Zhang, Xiaoning Hou, Hao Yin, Yuanwei Zhang, Qinghua Shi
Summary: This study found that RAD51AP2 plays a specific role in the formation of crossovers on XY chromosomes in the pseudoautosomal region, and its mutation leads to compromised DSB repair in PARs.
Review
Andrology
Yang Li, Yu-Fan Wu, Han-Wei Jiang, Ranjha Khan, Qi-Qi Han, Furhan Iqbal, Xiao-Hua Jiang, Qing-Hua Shi
Summary: This article discusses the formation of DNA double-strand breaks (DSBs) in meiosis and its importance for sexually reproducing organisms, as well as the potential infertility issues caused by abnormal DSB formation. Research has shown that the number and distribution of DSBs are tightly regulated, but abnormalities can still occur. By analyzing the mutations of DSB formation genes and their association with human infertility, future directions and strategies for studying meiotic DSB formation are proposed.
ASIAN JOURNAL OF ANDROLOGY
(2021)
Article
Genetics & Heredity
Miguel Hernandez Sanchez-Rebato, Alida M. Bouatta, Maria E. Gallego, Charles I. White, Olivier Da Ines
Summary: RAD54 protein plays a crucial role in repairing meiotic double strand breaks by RAD51 in the plant Arabidopsis thaliana, particularly in the absence of DMC1. Its function in meiosis is mainly downstream of the support provided by RAD51 to DMC1 activity. These findings provide new insights into the regulation of homologous recombination in plants.
Article
Multidisciplinary Sciences
Wu Zuo, Guangming Chen, Zhimei Gao, Shuai Li, Yanyan Chen, Chenhui Huang, Juan Chen, Zhengjun Chen, Ming Lei, Qian Bian
Summary: During meiosis, chromosomes undergo significant changes in morphology and intranuclear positioning. A study using Hi-C mapping in mouse spermatogenesis revealed the roles of transcriptional activity and mechanical force in homolog alignment and recombination.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Meng Lin, Jinxing Lv, Dan Zhao, Siyu Liu, Jinfu Xu, Yangyang Wu, Fuxin Wang, Jun Zhang, Bo Zheng, Cong Shen, Xie Guan, Jun Yu, Xiaoyan Huang
Summary: Given the importance of the MRN complex in HR, this study identifies MRNIP as a novel HR factor crucial for the expression of the MRN complex and loading of recombinases, thus promoting meiotic progression.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Dipak Walunj, Ebaston Thankarajan, Chandrashekhar Prasad, Helena Tuchinsky, Simone Baldan, Michael Y. Sherman, Leonid Patsenker, Gary Gellerman
Summary: The chimeric molecules created in this study successfully enhanced the inhibition activity towards DSB induced by Topo II, leading to more DSB and accelerated cancer cell killing. These chimeras exhibited higher antiproliferative activity compared to traditional treatment components, accumulated at tumor sites and showed stronger tumor suppressing activities.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Frederic Baudat, Bernard de Massy, Frederic Veyrunes
Article
Biochemistry & Molecular Biology
Frantzeskos Papanikos, Julie A. J. Clement, Erika Testa, Ramya Ravindranathan, Corinne Grey, Ihsan Dereli, Anastasiia Bondarieva, Sarai Valerio-Cabrera, Marcello Stanzione, Alexander Schleiffer, Petr Jansa, Diana Lustyk, Ji-Feng Fei, Ian R. Adams, Jiri Forejt, Marco Barchi, Bernard de Massy, Attila Toth
Article
Biology
Melen Leclerc, Julie A. J. Clement, Didier Andrivon, Frederic M. Hamelin
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2019)
Article
Biology
Yukiko Imai, Mathilde Biot, Julie A. J. Clement, Mariko Teragaki, Serge Urbach, Thomas Robert, Frederic Baudat, Corinne Grey, Bernard de Massy
Article
Urology & Nephrology
Nina Mann, Slim Mzoughi, Ronen Schneider, Susanne J. Kuhl, Denny Schanze, Verena Klambt, Svjetlana Lovric, Youying Mao, Shasha Shi, Weizhen Tan, Michael Kuhl, Ana C. Onuchic-Whitford, Ernestine Treimer, Thomas M. Kitzler, Franziska Kause, Sven Schumann, Makiko Nakayama, Florian Buerger, Shirlee Shril, Amelie T. van der Ven, Amar J. Majmundar, Kristina Marie Holton, Amy Kolb, Daniela A. Braun, Jia Rao, Tilman Jobst-Schwan, Eva Mildenberger, Thomas Lennert, Alma Kuechler, Dagmar Wieczorek, Oliver Gross, Beate Ermisch-Omran, Anja Werberger, Martin Skalej, Andreas R. Janecke, Neveen A. Soliman, Shrikant M. Mane, Richard P. Lifton, Jan Kadlec, Ernesto Guccione, Michael J. Schmeisser, Martin Zenker, Friedhelm Hildebrandt
Summary: Variants in the transcriptional regulator PRDM15 are found to cause proteinuric kidney disease in six families, with four of them displaying additional features of a GAMOS-like syndrome. All variants destabilize the PRDM15 protein, with the ZNF variant also interfering with transcriptional activation. Knockdown of Prdm15 in Xenopus embryos disrupts pronephric development, and CRISPR-mediated knockout of PRDM15 in human podocytes leads to dysregulation of renal developmental genes.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Multidisciplinary Sciences
Anne-Emmanuelle Foucher, Leila Touat-Todeschini, Ariadna B. Juarez-Martinez, Auriane Rakitch, Hamida Laroussi, Claire Karczewski, Samira Acajjaoui, Montserrat Soler-Lopez, Stephen Cusack, Cameron D. Mackereth, Andre Verdel, Jan Kadlec
Summary: In this study, the authors characterized the structural and functional features of three interfaces of the Red1 protein in the MTREC/PAXT nuclear complex, providing mechanistic insights into the conserved features of MTREC/PAXT architecture. These findings contribute to a better understanding of the mechanisms of RNA degradation.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Alexandre Nore, Ariadna B. Juarez-Martinez, Julie Clement, Christine Brun, Boubou Diagouraga, Hamida Laroussi, Corinne Grey, Henri Marc Bourbon, Jan Kadlec, Thomas Robert, Bernard de Massy
Summary: This study identifies REC114 as a direct partner of TOPOVIBL and demonstrates that their interaction is crucial for efficient and timely DNA double-strand break (DSB) activity during meiosis.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Hamida Laroussi, Ariadna B. Juarez-Martinez, Aline Le Roy, Elisabetta Boeri Erba, Frank Gabel, Bernard de Massy, Jan Kadlec
Summary: Meiotic recombination in mice is initiated by DNA double-strand breaks (DSBs), catalyzed by the TOPOVIL complex. This complex's activity is regulated by meiotic factors such as REC114, MEI4, and IHO1, but the mechanism is not well understood. This study reveals the molecular details of these factors and their interactions, including REC114 forming homodimers, associating with MEI4 as a heterotrimer, and IHO1 forming tetramers. The study also shows that IHO1 directly interacts with REC114 and shares a binding surface with TOPOVIBL and another meiotic factor ANKRD31. These findings suggest the existence of a ternary IHO1-REC114-MEI4 complex and highlight the potential regulatory role of REC114 in mediating interactions with multiple partners.
Article
Biology
Rajeev Kumar, Cecilia Oliver, Christine Brun, Ariadna B. Juarez-Martinez, Yara Tarabay, Jan Kadlec, Bernard de Massy
LIFE SCIENCE ALLIANCE
(2018)
Article
Biochemistry & Molecular Biology
Leila Touat-Todeschini, Yuichi Shichino, Mathieu Dangin, Nicolas Thierry-Mieg, Benoit Gilquin, Edwige Hiriart, Ravi Sachidanandam, Emeline Lambert, Janine Brettschneider, Michael Reuter, Jan Kadlec, Ramesh Pillai, Akira Yamashita, Masayuki Yamamoto, Andre Verdel