Article
Biochemical Research Methods
Chunlei Jiang, Hangyu Yue, Bing Yan, Taiji Dong, Xiangyu Cui, Peng Chen, Zengbo Wang
Summary: The study demonstrated the use of yeast cells as imaging lenses, trapping and magnifying nanoscale objects using fiber-based optical trapping technique. Experimental results showed that yeast cells could clearly resolve 100 nm features on a Blu-ray disc.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Multidisciplinary Sciences
Kaarjel K. Narayanasamy, Johanna Rahm, Siddharth Tourani, Mike Heilemann
Summary: DNA-PAINT image acquisition speed is slow. The authors utilized the neural network DeepSTORM to predict fluorophore positions from high emitter density DNA-PAINT data in order to achieve image acquisition in one minute; they demonstrated multi-color and large-area imaging of semi-thin neuronal tissue.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Kim Ann Saal, Ali H. Shaib, Nikolaos Mougios, Dagmar Crzan, Felipe Opazo, Silvio O. Rizzoli
Summary: Expansion microscopy (ExM) is improved by high-temperature homogenization (X10ht), which allows for the expansion of samples up to 10-fold in a single step. This method enables the use of multicolor stimulated emission depletion (STED) microscopy for high-resolution imaging and improves epitope preservation for labeling probes and signal amplification. X10ht holds promise for nanoscale resolution in biological samples.
SCIENTIFIC REPORTS
(2023)
Article
Physics, Applied
Tal I. Sommer, Ori Katz
Summary: The ultrasound pixel-reassignment (UPR) technique adapts the pixel-reassignment method from confocal fluorescent microscopy to improve resolution and signal to noise ratio in ultrasound imaging. The theoretical analysis and experimental evaluation demonstrate a 25% resolution improvement and 3 dB SNR improvement in in vitro scans without any hardware or acquisition scheme changes.
APPLIED PHYSICS LETTERS
(2021)
Article
Optics
Balakrishnan Viswanathan, Gabriela Barreto Lemos, Mayukh Lahiri
Summary: QIUP is a unique method of image acquisition using undetected photons, relying on quantum interference and spatial correlations between twin photons to form an image. The resolution limits of QIUP are determined by the spatial correlation between twin photons and cannot be further improved by conventional optical techniques.
Article
Optics
Youngrok Kim, Sungwoong Park, Hogil Baek, Sung-wook Min
Summary: This paper investigates the volumetric image characteristics of integral imaging display based on self-interference incoherent digital holography. It proposes a technique to estimate and analyze the voxel characteristics of the integral imaging system such as the depth location and resolution. The estimation method can be applied to the accurate and quantitative analysis of the volumetric characteristics of light field 3D displays.
Article
Chemistry, Physical
Shuang Zhang, Kirill Kniazev, Ilia M. Pavlovetc, Shubin Zhang, Robert L. Stevenson, Masaru Kuno
Summary: Infrared photothermal heterodyne imaging (IR-PHI) is an all-optical table top approach that enables super-resolution mid-infrared microscopy and spectroscopy. By using visible laser detection of photothermal changes induced by specimens' absorption of infrared radiation, IR-PHI achieves a spatial resolution improvement from about 300 to around 150 nm, overcoming the diffraction limit and background artifacts with the help of a deep learning network.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Optics
Lingjia Dai, Mingda Lu, Chao Wang, Sudhakar Prasad, Raymond Chan
Summary: Three-dimensional point source recovery from two-dimensional data is a challenging problem. This article proposes a convolution neural network-based approach to automatically localize space debris in full 3D space. The method is efficient and achieves higher precision compared to traditional model-based methods.
Article
Chemistry, Analytical
Yang Zhang, Gaoxiang Wang, Peizhou Huang, Edison Sun, Junghun Kweon, Qianru Li, Ji Zhe, Leslie L. Ying, Hao F. Zhang
Summary: This study developed a deep learning-based imaging analysis method to minimize molecular misidentification in three-color super-resolution imaging. By characterizing pure samples of different photoswitchable fluorophores and visualizing distinct subcellular proteins in cells, the researchers verified a 3-fold reduction in molecular misidentification in the new imaging method.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Benjamin Lochocki, Ksenia Abrashitova, Johannes F. de Boer, Lyubov Amitonova
Summary: Compressive imaging using sparsity constraints offers improved spatial resolution and overcomes traditional limits; the use of speckle illumination and single-pixel detection in experimental setups is crucial; oversampling may decrease the resolution and reconstruction quality of compressive imaging.
Article
Instruments & Instrumentation
Ki-Hee Song, Cheng Sun, Hao F. Zhang
Summary: Spectroscopic single-molecule localization microscopy (sSMLM) has become a key tool in functional super-resolution imaging by providing spatial and spectral information of single molecules at nanoscale resolution. A recently developed dual-wedge prism (DWP) imaging spectrometer has expanded the accessibility of sSMLM with improved imaging resolution and system reliability. Understanding the key system parameters is crucial for optimizing the DWP imaging spectrometer and achieving better multi-color functional imaging.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2023)
Article
Optics
Chuanzhen Hu, Ziling Jiang, Peng Liu, Yajun Yu, Kaiqin Chu, Zachary J. Smith
Summary: Traditional line-scan Raman imaging combines rapid imaging speed and complete spectral information, but is limited by diffraction-limited resolution. By using sinusoidally structured line excitation and a galvo-modulated structured line imaging system, it is possible to improve the lateral resolution of Raman imaging. Experimental results demonstrate a two-fold increase in lateral resolution and preservation of complete spectral information.
Article
Physics, Applied
Dekel Raanan, Man Suk Song, William A. Tisdale, Dan Oron
Summary: This study extends image scanning microscopy to second harmonic generation (SHG) by extracting the complex field amplitude of the second-harmonic beam, allowing for super-resolved phase-sensitive SHG microscopy.
APPLIED PHYSICS LETTERS
(2022)
Article
Optics
Bo Xiong, Tianyi Zhu, Yuhan Xiang, Xiaopeng Li, Jinqiang Yu, Zheng Jiang, Yihan Niu, Dong Jiang, Xu Zhang, Lu Fang, Jiamin Wu, Qionghai Dai
Summary: MiSLFM utilizes a tilted mirror to achieve super-resolved axial resolution with a single objective, expanding the depth of field of LFM. It allows observation of various organelle interactions and intercellular interactions, as well as more robust blood cell tracking in zebrafish larvae under low light conditions.
LIGHT-SCIENCE & APPLICATIONS
(2021)
Article
Multidisciplinary Sciences
Hongxia Li, Antony R. Warden, Jie He, Guangxia Shen, Xianting Ding
Summary: Expansion microscopy (ExM) improves resolution of conventional microscopy by physically expanding biological specimens. We demonstrate a new hydrogel and a detachable chip that enhance the reproducibility of this high-resolution imaging technology. It allows for imaging cellular ultrastructures that cannot be resolved by conventional microscopy.
Editorial Material
Cell Biology
Nan Jiang, Jay Z. Parrish
DEVELOPMENTAL CELL
(2015)
Article
Cell Biology
Ximing Shao, Yi Liu, Qian Yu, Zhihao Ding, Wenyu Qian, Lei Zhang, Jianchao Zhang, Nan Jiang, Linfei Gui, Zhiheng Xu, Yang Hong, Yifan Ma, Yanjie Wei, Xiaoqing Liu, Changan Jiang, Minyan Zhu, Hongchang Li, Huashun Li
Article
Behavioral Sciences
E. E. Blue, C. -E. Yu, T. A. Thornton, N. H. Chapman, E. Kernfeld, N. Jiang, K. M. Shively, K. J. Buckingham, C. T. Marvin, M. J. Bamshad, T. D. Bird, E. M. Wijsman
GENES BRAIN AND BEHAVIOR
(2018)
Article
Developmental Biology
Nan Jiang, Peter Soba, Edward Parker, Charles C. Kim, Jay Z. Parrish
Article
Multidisciplinary Sciences
Nina Boiko, Geraldo Medrano, Elizabeth Montano, Nan Jiang, Claire R. Williams, Ngonidzashe B. Madungwe, Jean C. Bopassa, Charles C. Kim, Jay Z. Parrish, Kenneth M. Hargreaves, James D. Stockand, Benjamin A. Eaton
Article
Genetics & Heredity
Ranad Shaheen, Nan Jiang, Fatema Alzahrani, Nour Ewida, Tarfa Al-Sheddi, Eman Alobeid, Damir Musaev, Valentina Stanley, Mais Hashem, Niema Ibrahim, Firdous Abdulwahab, Abduljabbar Alshenqiti, Fatma Mujgan Sonmez, Nadia Saqati, Hamad Alzaidan, Mohammad M. Al-Qattan, Futwan Al-Mohanna, Joseph G. Gleeson, Fowzan S. Alkuraya
AMERICAN JOURNAL OF HUMAN GENETICS
(2019)
Article
Biology
Nan Jiang, Jeffrey P. Rasmussen, Joshua A. Clanton, Marci F. Rosenberg, Kory P. Luedke, Mark R. Cronan, Edward D. Parker, Hyeon-Jin Kim, Joshua C. Vaughan, Alvaro Sagasti, Jay Z. Parrish
Meeting Abstract
Hematology
Rashmi Kanagal-Shamanna, Shruti Rao, Panieh Terraf, Gordana Raca, Jason Saliba, Arpad Danos, Coumarane Mani, Heather Williams, Nan Jiang, Kevin C. Vavra, Liying Zhang, Zonggao Shi, Peng Li, Yuwen Li, Madhu Michael Ouseph, Ella R. Thompson, Yiming Zhong, Nicole Hinceman, Rong He, Kilannin Krysiak, Alex Wagner, Matthew Mccoy, Mariam T. Matthew, Kristin Deeb, Fengli Zhang, Jie Liu, David S. Viswanatha, Piers Blombery, David Wu, Shashikant Kulkarni, Subha Madhavan, Xinjie Xu