期刊
MOLECULAR AND CELLULAR PROBES
卷 40, 期 -, 页码 40-43出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2018.06.002
关键词
GI norovirus; Oyster; Primer; Specificity; Coverage; Sensitivity
类别
资金
- National Natural Science Foundation of China [31601570, 31570112]
- National Key Research and Development Program of China [SQ2017YFC160114]
A pair of nested PCR universal primers (NGIOF and NGIOR) specific for genogroup I (GI) noroviruses was designed based on all GI sequences available in public databases. The primers were evaluated for their specificity, sensitivity and coverage, which demonstrate their reliable performance upon detection of GI noroviruses in oysters.
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