Article
Obstetrics & Gynecology
A. Kedem, K. Ulanenko-Shenkar, Y. Yung, M. Youngster, S. Avraham, G. M. Yerushalmi, A. Hourvitz
Summary: This study confirms LUM as a newly discovered ovulatory gene that may play a crucial role in the preovulatory period leading up to ovulation, as well as in endometriosis infertility. A better understanding of LUM's role could potentially offer new treatment paradigms for certain types of female infertility.
REPRODUCTIVE SCIENCES
(2022)
Article
Reproductive Biology
Xue Yang, Jun Ma, Luoyu Mo, Yan Xiong, Xianrong Xiong, Daoliang Lan, Wei Fu, Shi Yin
Summary: This study found that stanniocalcin-1 (STC1) is widely expressed in yak ovaries and plays a role in follicular development and hormone production. Inhibiting STC1 expression results in decreased proliferation and survival of yak granulosa cells and increased secretion of estrogen. Overexpression of STC1 partially restores granulosa cell proliferation and has opposite effects on progesterone and estrogen secretion. This study provides new insights into the role of STC1 in yak granulosa cells and the mechanism of yak reproduction.
Article
Reproductive Biology
Yu Zhang, Jun Zhang, Junming Sun, Yiqiang Ouyang, Deshun Shi, Fenghua Lu
Summary: In this study, the effects of hypoxia on estrogen synthesis in buffalo granulosa cells (GCs) were examined. The results showed that hypoxia enhanced the expression levels of estrogen synthesis-related genes and the secretion levels of estradiol in buffalo GCs, and increased the sensitivity of GCs to FSH. In addition, the inhibition of cAMP/PKA signaling pathway reduced estrogen synthesis and estradiol secretion, and decreased the responsiveness of buffalo GCs to FSH under hypoxic conditions.
Article
Endocrinology & Metabolism
Xiaotong Wang, Elaine Lee, Barbara F. Hales, Bernard Robaire
Summary: Organophosphate esters (OPEs) are widely used as flame retardants and plasticizers and can be found everywhere. Exposure to these chemicals may disrupt female sex hormone balance and affect female fertility. In this study, the effects of OPEs on KGN ovarian granulosa cells were investigated. OPEs altered the steroidogenic ability of these cells by dysregulating the expression of genes involved in steroid and cholesterol biosynthesis. These effects may have negative impacts on female reproduction.
Article
Cell Biology
Jung-Chien Cheng, Xiaoyu Han, Qingxue Meng, Yanjie Guo, Boqun Liu, Tinglin Song, Yuanyuan Jia, Lanlan Fang, Ying-Pu Sun
Summary: This study reveals that HB-EGF can enhance the luteal function in humans by inducing StAR expression and progesterone production through the activation of the ERK1/2 signaling pathway.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Endocrinology & Metabolism
Hai-Yun Guan, He-Xia Xia, Xiu-Ying Chen, Lu Wang, Zhi-Jing Tang, Wei Zhang
Summary: The activation of TLR4 was found to suppress CYP19A1 expression and E2 secretion in human granulosa cells via NF-κB signaling, which has implications for follicular development and estrogen synthesis regulation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
Loise Serra, Anthony Estienne, Giovanni Caria, Christelle Rame, Claudy Jolivet, Claire Froger, Abel Henriot, Laurence Amalric, Fabrice Guerif, Pascal Froment, Joelle Dupont
Summary: Five triazoles were found in soil or water samples from the Centre-Val de Loire region of France, and their effects on steroidogenesis in ovarian cells were studied. These triazoles reduced the secretion of progesterone and estradiol at non-cytotoxic doses.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Blazej Chermula, Wieslawa Kranc, Piotr Celichowski, Boguslawa Stelmach, Hanna Piotrowska-Kempisty, Paul Mozdziak, Leszek Pawelczyk, Robert Zygmunt Spaczynski, Bartosz Kempisty
Summary: This study analyzes the epithelial origin of cumulus and granulosa cells in ovarian follicles, and identifies genes associated with epithelialization processes. The results suggest that these cells originate from the ovarian surface epithelium, and some gene expression profiles may have tumorigenic properties.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Arjoune Asma, Sirard Marc-Andre
Summary: This study found that melatonin may regulate metabolic processes during folliculogenesis by activating PKA and PKB signaling pathways, thereby slowing down follicular growth and differentiation and preventing follicular atresia and early luteinization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Francesca P. Luongo, Filippo Dragoni, Adele Boccuto, Eugenio Paccagnini, Mariangela Gentile, Tamara Canosi, Giuseppe Morgante, Alice Luddi, Maurizio Zazzi, Ilaria Vicenti, Paola Piomboni
Summary: This study investigates the potential of SARS-CoV-2 to infect follicular ovarian cells and finds that these cells are susceptible to the virus. This suggests that COVID-19 infection may have a detrimental effect on female fertility.
Review
Cell Biology
Claudia Dompe, Magdalena Kulus, Katarzyna Stefanska, Wieslawa Kranc, Blazej Chermula, Rut Bryl, Wojciech Pienkowski, Mariusz J. Nawrocki, James N. Petitte, Boguslawa Stelmach, Paul Mozdziak, Michal Jeseta, Leszek Pawelczyk, Jedrzej M. Jaskowski, Hanna Piotrowska-Kempisty, Robert Z. Spaczynski, Michal Nowicki, Bartosz Kempisty
Summary: The ovarian follicle is the basic functional unit of the ovary, consisting of theca cells and granulosa cells. Granulosa cells can be further divided into mural granulosa cells and cumulus cells, each playing different roles during folliculogenesis. Both types of cells exhibit stem-like properties and are influenced by various miRNAs, which may contribute to female fertility issues such as PCOS or POI. Further research on the cellular features and molecular pathways of granulosa and cumulus cells could lead to new strategies in enhancing assisted reproductive techniques.
Article
Oncology
Xue Jiao, Xiruo Zhang, Nianyu Li, Dunfang Zhang, Shidou Zhao, Yujie Dang, Peter Zanvit, Wenwen Jin, Zi-Jiang Chen, Wanjun Chen, Yingying Qin
Summary: This study revealed an augmented T helper 1 (T(H)1) response and regulatory T (T-reg) cell deficiency in patients with premature ovarian insufficiency (POI), which was strongly correlated with disease severity. In mouse models, increased infiltration of T(H)1 cells in the ovary led to follicle atresia and ovarian insufficiency, while T-reg cells were able to prevent and reverse these effects. Furthermore, interferon (IFN) -gamma and tumor necrosis factor (TNF) -alpha were found to cooperate in promoting granulosa cell apoptosis and suppressing steroidogenesis in the context of POI.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Xianrong Xiong, Yulei Hu, Bangting Pan, Yanjin Zhu, Xixi Fei, Qinhui Yang, Yumian Xie, Yan Xiong, Daoliang Lan, Wei Fu, Jian Li
Summary: This study found that RFRP-3 regulates apoptosis and steroidogenesis in yak cumulus cells (CCs), as well as affects the developmental potential of yak oocytes, by binding with specific G protein-coupled receptors (GPRs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
Er-Meng Gao, Bongkoch Turathum, Ling Wang, Di Zhang, Yu-Bing Liu, Rong-Xin Tang, Ri-Cheng Chian
Summary: This study evaluated the differences in metabolites between cumulus cells (CCs) and mural granulosa cells (MGCs) from human preovulatory follicles. The metabolomics analysis revealed significantly different metabolites related to cholesterol transport, estradiol production, and antiapoptosis between CCs and MGCs. These differences may play a role in regulating oocyte maturation.
REPRODUCTIVE SCIENCES
(2022)
Article
Endocrinology & Metabolism
Marah Armouti, Miriam Rodriguez-Esquivel, Carlos Stocco
Summary: This study investigates the molecular mechanisms of Sik in inhibiting FSH actions in primary rat granulosa cells (GCs), and finds that SIK inhibition enhances the activation of the FSH signaling pathway. It also suggests that SIKs regulate the FSH pathway by affecting factors between cAMP/PKA and CREB.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Toxicology
Dragana Samardzija Nenadov, Biljana Tesic, Svetlana Fa, Kristina Pogrmic-Majkic, Dunja Kokai, Bojana Stanic, Nebojsa Andric
Summary: The study found that only long-term exposure to the mixture of endocrine disruptors MIX can alter steroidogenesis in HGrC1 cells. This effect was observed a few weeks into exposure and could be transient.
TOXICOLOGY IN VITRO
(2022)
Article
Toxicology
Bojana Stanic, Dunja Kokai, Biljana Tesic, Svetlana Fa, Dragana Samardzija Nenadov, Kristina Pogrmic-Majkic, Nebojsa Andric
Summary: By integrating experimental and in silico approaches, this study investigated the underlying mechanisms of dibutyl phthalate (DBP) induced vascular dysfunction. The findings suggest that DBP exposure can affect extracellular matrix, cell adhesion, and migration, which are associated with cardiovascular, cerebrovascular, and immune system diseases.
TOXICOLOGY LETTERS
(2022)
Article
Toxicology
Kristina Pogrmic-Majkic, Dragana Samardzija Nenadov, Biljana Tesic, Svetlana Fa Nedeljkovic, Dunja Kokai, Bojana Stanic, Nebojsa Andric
Summary: This study utilized AOPs and AOP networks to understand the impact of chemicals on human health. It derived the HFRT-AOP network and mapped DEHP to this network, providing insights into the toxic mechanism of DEHP-induced human female reproductive toxicity.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Cell Biology
Dragana Samardzija Nenadov, Kristina Pogrmic-Majkic, Biljana Tesic, Dunja Kokai, Svetlana Fa Nedeljkovic, Bojana Stanic, Nebojsa Andric
Summary: In this study, human granulosa cells were exposed to low levels of DEHP for an extended period of time to investigate its impact on cell function. The results showed no effect on steroidogenesis, but DEHP caused specific changes in genes and functions in the cells. SULT1A3 and SULT1A4, involved in catecholamine metabolism, were found to be the most affected genes. This study provides new insights into the relationship between DEHP, SULT1A3, and SULT1A4 in human granulosa cells.
Article
Biochemistry & Molecular Biology
Dunja Kokai, Bojana Stanic, Biljana Tesic, Dragana Samardzija Nenadov, Kristina Pogrmic-Majkic, Svetlana Fa Nedeljkovic, Nebojsa Andric
Summary: This study found that DBP can induce pro-angiogenic effects on human vascular endothelial cells, and this effect is mediated by the activation of ERK1/2, PI3K-Akt, and NO signaling pathways through ER and GPER dependent mechanisms.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Cell Biology
Biljana Tesic, Dragana Samardzija Nenadov, Tamara Tomanic, SvetlanaFa Nedeljkovic, Stevan Milatovic, Bojana Stanic, Kristina Pogrmic-Majkic, Nebojsa Andric
Summary: DEHP is an endocrine disruptor that affects steroidogenesis in human and rodent granulosa cells. This study analyzed the mechanism by which DEHP affects steroidogenesis in FSH-stimulated human granulosa cells. The results showed that DEHP decreased FSH-stimulated STAR and CYP19A1 expression, leading to a decrease in progesterone and estradiol production. DEHP also inhibited the cAMP and ERK1/2 signaling pathways, resulting in the inhibition of steroidogenesis.
Article
Toxicology
Bojana Stanic, Nemanja Milosevic, Natasa Sukur, Dragana Samardzija Nenadov, Svetlana Fa Nedeljkovic, Srdan Skrbic, Nebojsa Andric
Summary: By analyzing toxicogenomic data, we inferred the molecular pathway network involved in hepatocellular carcinoma (HCC) induced by aflatoxin B1 (AFB1). Computational tools were utilized to identify protein-protein interaction network, hub genes, transcription factors (TFs), upstream kinases, and biological processes (BPs). Our results revealed an association between AFB1 and hub genes involved in the cell cycle, and we identified TFs that regulate these hub genes and linked them with upstream kinases. This study provides important insights into the signaling hierarchy and information flow contributing to AFB1-induced HCC.
TOXICOLOGY MECHANISMS AND METHODS
(2023)
Article
Biochemistry & Molecular Biology
Bojana Stanic, Dunja Kokai, Jelena Markovic Filipovic, Dragana Samardzija Nenadov, Kristina Pogrmic-Majkic, Nebojsa Andric
Summary: This study investigates the effects of long-term low-level exposure to BPA on gene expression in human endothelial cells. The results indicate that BPA exposure leads to concentration-specific changes in gene expression, which are controlled by transcription factors rather than the estrogen receptor pathway.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Food Science & Technology
Natasa Sukur, Nemanja Milosevic, Kristina Pogrmic-Majkic, Bojana Stanic, Nebojsa Andric
Summary: Experimental evidence suggests that certain chemicals, especially endocrine disruptors, can have negative effects on the female reproductive system and decrease fertility in women. However, there is limited or no experimental data available for many chemicals regarding their impact and mechanisms of action in the female reproductive system. To predict the hazards of chemicals to the female reproductive system, a previously defined adverse outcome pathway (AOP) and Convolutional Deep Neural Network models were used. The models, trained using CompTox assays with intended molecular and biological targets corresponding to AOP7, achieved high performance in identifying potentially harmful chemicals. This approach provides a solution for quickly analyzing data and generating machine learning models to identify active chemicals in the female reproductive system. It may also be applicable to other chemicals and adverse outcome pathways if sufficient data is available.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Reproductive Biology
Dragana Samardzija Nenadov, Biljana Tesic, Tamara Tomanic, Marija Opacic, Bojana Stanic, Kristina Pogrmic-Majkic, Nebojsa Andric
Summary: This study examined the effects of different concentrations of DEHP exposure on human granulosa cells. The results showed that low concentration did not affect gene expression, while high concentration only affected the expression of nine genes. Despite these changes, progesterone production was not affected in both low and high concentration DEHP-exposed cells. Overall, this study suggests that acute exposure to low concentration of DEHP does not compromise the function of human granulosa cells, while high concentration only causes subtle effects. The identified nine novel targets of high-dose DEHP require further investigation for their role and importance in DEHP-exposed human granulosa cells.
REPRODUCTIVE TOXICOLOGY
(2023)
Article
Cell Biology
Karina A. Pena, Sofya Savransky, Breanna Lewis
Summary: Compartmentalization of GPCR signaling is an emerging topic that emphasizes the importance of spatial bias in signaling for physiological relevance. PTH1R was the first GPCR discovered to signal via cAMP from endosomes, challenging the conventional model of GPCR signaling. The location of cAMP generation determines the physiological outcomes of GPCR signaling.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Cheng-Xu Ma, Xiao-Ni Ma, Jin-Jin Liu, Cong-Hui Guan, Ying-Dong Li, Nan Zhao, Didac Mauricio, Song-Bo Fu
Summary: The downregulation of primary cilia (PCs) due to BRAFV600E mutation contributes to the aggressiveness and lymph node metastasis of Papillary Thyroid Cancer (PTC).
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Kejun Wu, Cuihua Huang, Wenrong Zheng, Yubin Wu, Qintao Huang, Menghua Lin, Ruonan Gao, Liqin Qi, Guanlian He, Xiaoying Liu, Xiaohong Liu, Linxi Wang, Zhou Chen, Libin Liu
Summary: Recurrent non-severe hypoglycemia in patients with diabetes is associated with cognitive impairment. This study found that this condition is associated with reduced mitophagy in the hippocampus, leading to mitochondrial dysfunction and neurological impairment.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Yifei Lv, Yizhou Huang, Huiyu Fan, Yunxiu Zhao, Linjuan Ma, Yibing Lan, Chunming Li, Peiqiong Chen, Zheng Lou, Jianhong Zhou
Summary: Before menopause, females have a lower incidence of cardiovascular disease than age-matched males, possibly due to the protective effects of sex hormones. 17 beta-E2 inhibits THBS1 expression, preventing cell senescence and apoptosis, and counteracts oxidative stress by suppressing the TGF-beta/Smad signaling pathway.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Ana C. L. Camargo, Flavia B. Constantino, Sergio A. A. Santos, Ketlin T. Colombelli, Luiz M. F. Portela, Matheus N. Fioretto, Luisa A. Barata, Guilherme T. Valente, Carlos S. Moreno, Luis A. Justulin
Summary: This study examined the effects of maternal malnutrition on the transcriptomic landscape of the ventral prostate in rats. It found that changes in molecular pathways related to cellular development and tissue morphogenesis were associated with maternal malnutrition. The Abcg1 gene was found to be deregulated in both malnourished rats and prostate cancer models and patients.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Sandra Romero-Cordoba, Mayel Chirinos, Nancy Noyola-Martinez, Nayeli Torres-Ramirez, Mitzi Garcia-Olivares, Juan Pablo Aragon-Hernandez, Ixchel Ramirez-Camacho, Rosa Zuniga, Fernando Larrea, Ali Halhali, David Barrera
Summary: This study analyzed the effects of calcitriol, TGF-131, and their combination on human trophoblast cells. The results showed that the combination treatment modified the transcriptional landscape and mainly affected the storage, activity, and metabolism of lipids, which may have an impact on placental development.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Traver J. Wright, Richard B. Pyles, Melinda Sheffield-Moore, Rachel R. Deer, Kathleen M. Randolph, Kristen A. Mcgovern, Christopher P. Danesi, Charles R. Gilkison, Weston W. Ward, Jayson A. Vargas, Peyton A. Armstrong, Sarah E. Lindsay, Mohammed F. Zaidan, Justin Seashore, Tamara L. Wexler, Brent E. Masel, Randall J. Urban
Summary: This study investigates the persistent neurologic symptoms in COVID-19 patients after recovery and explores the association between these symptoms and disrupted growth hormone secretion and gastrointestinal discomfort.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
Article
Cell Biology
Li-Li Chen, Ya-Qin Li, Zhi-Hui Kang, Xuan Zhang, Su-Yan Gu, Na Wang, Xue-Yan Shen
Summary: Defects in trophoblastic EMT caused by dysregulation of circTNRC18's interaction with LIN28A play a vital role in the development of preeclampsia. LIN28A overexpression suppresses circTNRC18-mediated inhibition of trophoblast migration, invasion, and EMT, while LIN28A knockdown promotes them. Furthermore, circTNRC18 regulates the intracellular distribution of LIN28A and the expression of insulin-like growth factor II, affecting cell migration and invasion. Targeting the circTNRC18-LIN28A regulatory axis may provide a novel treatment approach for preeclampsia.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2024)
