期刊
MICROBIOLOGY-SGM
卷 164, 期 3, 页码 349-358出版社
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.000608
关键词
vaginal probiotics; Lactobacillus spp.; Gardnerella vaginalis; Candida albicans; HeLa cells; pro-inflammatory cytokines
类别
资金
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [CBB-APQ-00984-16]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Lactobacilli are the dominant bacteria of the vaginal tract of healthy women and they play a major role in the maintenance of mucosal homeostasis, preventing genital infections, such as bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC). It is now known that one mechanism of this protection is the influence that lactobacilli can exert on host immune responses. In this context, we evaluated two Lactobacillus strains (L. plantarum 59 and L. fermentum 137) for their immunomodulatory properties in response to Gardnerella vaginalis (BV) or Candida albicans (VVC) infections in a HeLa cell infection model. G. vaginalis and C. albicans triggered the secretion of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6 and IL-8) and the activation of NF-kappa B in HeLa cells, in contrast to L. plantarum 59 and L. fermentum 137. Treatments with the Lactobacillus strains or their cell-free supernatants before (pre-treatment) or after (post-treatment) the challenge with the pathogens resulted in decreased secretion of pro-inflammatory cytokines and decreased activation of NF-kappa B. The treatments with Lactobacillus strains not only decreased the secretion of IL-8, but also its expression, as confirmed by gene reporter luciferase assay, suggesting transcription-level control by lactobacilli. In conclusion, L. plantarum 59 and L. fermentum 137 were confirmed to have an anti-inflammatory effect against G. vaginalis and C. albicans and they were able to influence signalling in NF-kappa B pathway, making them interesting candidates as probiotics for the prevention or treatment of BV and VVC.
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