期刊
MICROBIOLOGICAL RESEARCH
卷 207, 期 -, 页码 177-187出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2017.11.008
关键词
Streptococcus suis; Spermidine; Spermine; Putrescine; Polyamine uptake; Peptidoglycan (PG)
类别
资金
- National Key Research and Development Plans [2017YFD0500201]
- National Natural Science Foundation of China [31472202, 31572535]
- National Basic Research Program of China (973 Program) [2012CB518802]
- Hubei Province Natural Science Foundation for Innovative Research Groups [2016CFA015]
Spermidine (Spd), spermine (Spm), and putrescine (Put), which are the most widely distributed cellular poly amines, are essential for normal growth and multiplication of both eukaryotic and prokaryotic cells. In this study, we identified the only putative polyamine transport system PotABCD in Streptococcus suis, a worldwide zoonotic Gram-positive pathogen causing lethal infections in humans and pigs. It was discovered that S. suis could uptake polyamines preferably Spd and Spm. By constructing a potA deleted mutant, we confirmed that PotABCD was responsible for polyamine uptake, and PotD bound to the protein of polyamines. The four PotABCD genes were co-transcribed with murB, a gene involved in peptidoglycan (PG) synthesis. Furthermore the roles of polyamine transport system in maintaining the PG structure were detected to understand the biological significance of this co-transcription. In contrast to the wild type, the mutant ApotA exhibited elongated chain length and abnormal cell division morphology. Phenotypic changes were attributed to be the up-regulation of genes involved in PG synthesis and hydrolysis in ApotA. Additionally, polyamines functioned not only as feedback regulators of PotA by inhibiting PotA activity but also as regulators on potABCD and genes involved in PG synthesis. This study reveals the functions of PotABCD in polyamine transport and the regulatory roles of polyamines in PG synthesis. Results provide new insights into the machineries contributing to normal growth and cell division of S. suis.
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