Article
Microbiology
Dianwen Xu, Guiqiu Hu, Jianchun Luo, Ji Cheng, Di Wu, Lisha Cheng, Xuejie Huang, Shoupeng Fu, Juxiong Liu
Summary: This study investigates the role and mechanism of mitophagy in the intracellular survival of Staphylococcus aureus (S.aureus) within bovine mammary epithelial cells (BMECs). The findings showed that S.aureus induced complete mitophagy to promote its survival within BMECs, which was mediated by the activation of the p38-PINK1-Parkin signaling pathway.
VETERINARY MICROBIOLOGY
(2023)
Article
Immunology
Haojun Xu, Changjie Lin, Ting Li, Yifan Zhu, Jinghan Yang, Sijie Chen, Jianguo Chen, Xi Chen, Yingyu Chen, Aizhen Guo, Changmin Hu
Summary: In this study, the researchers aimed to investigate the effect of Staphylococcus aureus and Escherichia coli on m(6)A-modified circRNAs in bovine mammary epithelial cells. They found that these modified circRNAs were associated with cell injury, inflammation, apoptosis, and autophagy, and identified their regulatory roles in MAPK, WNT, and inflammation pathways.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Mara D. Saenz-de-Juano, Giulia Silvestrelli, Andres Weber, Christian Rohrig, Mathias Schmelcher, Susanne E. Ulbrich
Summary: This study investigates the impact of Staphylococcus aureus extracellular vesicles on mammary epithelial cells, providing insights into how the bacteria evades host defense mechanisms and colonizes the mammary gland effectively.
Article
Veterinary Sciences
Bruno Toledo-Silva, Ana Claudia Dumont Oliveira, Fernando N. Souza, Freddy Haesebrouck, Sarne De Vliegher
Summary: This study found that cell-free supernatants from bovine non-aureus staphylococcal isolates can prevent adhesion and internalization of Staphylococcus aureus into bovine mammary epithelial cells, potentially through the S. aureus accessory gene regulator system. These findings provide new anti-virulence strategies for treating and controlling bovine S. aureus mastitis.
VETERINARY RESEARCH
(2023)
Article
Veterinary Sciences
Xiaozhou Wang, Mingchao Liu, Na Geng, Yongzhen Du, Zhaoming Li, Xin Gao, Bo Han, Jianzhu Liu, Yongxia Liu
Summary: Cell death and inflammation are closely linked during mastitis caused by Staphylococcus aureus. Bovine mammary epithelial cells respond to S. aureus by NLRP3 inflammasome activation, leading to cell apoptosis and pyroptosis. This cell death process is associated with caspase-1 activation.
VETERINARY RESEARCH
(2022)
Article
Microbiology
Marco Antonio Barajas-Mendiola, Maria Guadalupe Salgado-Lora, Joel Edmundo Lopez-Meza, Alejandra Ochoa-Zarzosa
Summary: This study investigates the epigenetic regulation of bovine mammary epithelial cells (bMECs) challenged with Staphylococcus aureus induced by bovine prolactin (bPRL). The results show that bPRL alters specific epigenetic marks, affects the expression of inflammatory genes, and regulates the expression of miRNAs, allowing S. aureus to persist and evade the host immune response.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Kangjun Liu, Xi Zhou, Li Fang, Junsheng Dong, Luying Cui, Jun Li, Xia Meng, Guoqiang Zhu, Jianji Li, Heng Wang
Summary: Staphylococcus aureus (S. aureus) has the ability to invade mammalian cells and protect itself from clearance by the immune system. The role of mitophagy in S. aureus-associated bovine mastitis has been unclear. This study found that S. aureus infection induces mitophagy and enhances inflammation, contributing to S. aureus survival.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biology
Shamsaldeen Ibrahim Saeed, Erkihun Aklilu, Khalid M. Mohammedsalih, Adewole A. Adekola, Ahmed Elmontaser Mergani, Maizan Mohamad, Nor Fadhilah Kamaruzzaman
Summary: The study evaluated the potential antibacterial properties of ikarugamycin as a novel antibiotic candidate against intracellular persistent infection caused by Staphylococcus aureus, highlighting its intracellular killing activity and potential application in treating S. aureus mastitis in the future.
Article
Veterinary Sciences
Yuhao Chen, Yuze Ma, Qiang Ji, Xiaoru Yang, Xue Feng, Ruiyuan Yao, Xiaoou Cheng, Tingting Li, Yanfeng Wang, Zhigang Wang
Summary: The study revealed that S. aureus infection decreases milk protein synthesis in bovine mammary epithelial cells, reducing amino acid uptake, impairing mTORC1 signaling, and inhibiting the activity of NF-kappa B and STAT5.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Yi-Tian Ying, Wei-Jia Ren, Xun Tan, Jing Yang, Rui Liu, Ai-Fang Du
Summary: The membrane-binding protein Annexin A2 plays a role in the internalization of Staphylococcus aureus into bovine mammary epithelial cells, with the interaction between ClfB and AnxA2 promoting the invasion of S. aureus. Targeting this interaction may provide protection against S. aureus mastitis.
Article
Cell Biology
Yi-Tian Ying, Jing Yang, Xun Tan, Rui Liu, Ying Zhuang, Jia-Xue Xu, Wei-Jia Ren
Summary: Escherichia coli and Staphylococcus aureus induce distinct immune and inflammatory responses in dairy cattle mastitis, with Nrf2 pathway playing a key role in this difference. Activation of Nrf2 is essential for bovine mammary epithelial cells to mount an effective innate defensive response, while Staphylococcus aureus impairs Nrf2 activation leading to a weaker inflammatory response.
Article
Biotechnology & Applied Microbiology
Xiao-Yan Zhu, Meng-Ling Wang, Meng Cai, Xue-Mei Nan, Yi-Guang Zhao, Ben-Hai Xiong, Liang Yang
Summary: Mastitis is a common infectious disease in dairy farms that results in decreased milk production and quality. Staphylococcus aureus is one of the main bacteria causing subclinical mastitis. Exosomes play a role in various physiological and pathological functions by containing proteins, lipids, and nucleic acids.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ruiyuan Yao, Manshulin Wang, Yue Zhao, Qiang Ji, Xue Feng, Linfeng Bai, Lili Bao, Yanfeng Wang, Huifang Hao, Xihe Li, Zhigang Wang
Summary: This study reveals the regulation of lipid synthesis under Staphylococcus aureus infection through mTORC1 signaling and identifies the protective effects of CGA on lipid synthesis in bovine mammary epithelial cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Immunology
Bo Liu, Qianru Li, Zhiguo Gong, Jiamin Zhao, Baichen Gu, Shuang Feng
Summary: This study found that lipoproteins from Staphylococcus aureus play a crucial role in inducing inflammatory responses and mediating bacterial internalization into bovine mammary epithelial cells.
MICROBIAL PATHOGENESIS
(2022)
Article
Veterinary Sciences
Mingcheng Cai, Wenqiao Fan, Xiaoying Li, Hanchang Sun, Liuliu Dai, Defang Lei, Ying Dai, Yuhua Liao
Summary: Mastitis, an inflammatory disease mainly caused by bacteria, leads to severe economic loss in the dairy industry. Research showed that LTA can activate inflammatory responses via the TLR2/MyD88/PI3K/AKT signaling pathway, while miR-23a inhibits this response by targeting PI3K. Additionally, Mac-T cell-derived exosomes may be associated with inflammatory responses by promoting M1 polarization of bovine macrophages.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Camila Beccaria, Natalia S. Velazquez, Victoria Chervaz, Silvana Pirola, Celina Baravalle, Maria S. Renna, Luis F. Calvinho, Bibiana E. Dallard
Summary: The study revealed that during active involution, the expression and activity of MMP-2 and -9 in mammary tissue were significantly higher in S. aureus-infected quarters compared with uninfected quarters, along with increased expression of TIMP-1 and -2. The imbalance of MMPs/TIMPs could lead to more severe damage to mammary tissue in S. aureus-infected quarters.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Anatomy & Morphology
Eduardo M. Belotti, Sofia C. Sacco, Antonela F. Stassi, Ulises S. Notaro, Emmanuel Angeli, Lucas Etchevers, Juan A. Chiaraviglio, Hugo H. Ortega, Natalia R. Salvetti
Summary: This study aimed to describe a case of incipient formation of granulosa cell tumor (GCT) in a 7-year-old Holstein cow and characterize it by its immunohistochemical pattern and hormonal profile. The research found that a highly differentiated pattern could be determined through a panel of immunohistochemical markers in the GCT concomitant with normal follicular development. This study provides new insights into GCTs in cattle before ovarian function is compromised.
ANATOMIA HISTOLOGIA EMBRYOLOGIA
(2022)
Article
Food Science & Technology
Lucas Emanuel Ribas, Maria Eugenia Baravalle, Fatima Belen Gasser, Maria Sol Renna, Silvina Addona, Hugo Hector Ortega, Graciela Hilda Savino, Franco Van de Velde, Gustavo Juan Hein
Summary: The study found that the Marameck variety of pecan nutshells had higher total phenolic compound content than the Mahan variety, and the optimization of extraction conditions was crucial for the extraction of TPC. The extracts showed strong cytotoxic effects against colon cancer cells in vitro, primarily inducing cell death through apoptosis.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Facundo Salinas, Belkis E. E. Marelli, Santiago Sanguineti, Fernando Goldbaum, Luciana Munoz, Lucas Etchevers, Paula Silvestrini, Ulises S. S. Notaro, Natalia R. R. Salvetti, Vanesa Zylberman, Hugo H. H. Ortega
Summary: This study investigated the preclinical safety and biodistribution of CoviFab, a RBD-specific F(ab')2 fragment derived from equine polyclonal antibodies. The results showed that CoviFab had a safe biodistribution in mice without observable adverse effects.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Polymer Science
Karina L. Bierbrauer, Roxana Alasino, Fernando E. Barclay, Eduardo M. Belotti, Hugo H. Ortega, Dante M. Beltramo
Summary: The functional capability of a cross-linked hydrogel composed of sulfated glycosaminoglycans and a cationic cellulose for treating osteoarthritis was demonstrated in experimental animal models. In long-term follow-up after implantation, the hydrogel showed superior safety, permanence, and reduced cartilage damage compared to commercial hyaluronic acid.
Article
Agriculture, Dairy & Animal Science
D. Barcarolo, E. Angeli, L. E. Ribas, S. M. Addona, H. H. Ortega, G. J. Hein
Summary: In dairy cows, oxidative stress during the transition period can be influenced by environmental conditions such as heat stress. A study developed and validated a method for accurate determination of 3-NT in blood, urine, and liver samples in dairy cows using LC-MS/MS. Results showed higher 3-NT concentrations in cows that gave birth during spring, along with higher BHBA and lower glucose concentrations. Liver TAG content and aspartate transaminase activity were also higher in cows that gave birth during spring.
Review
Reproductive Biology
A. F. Stassi, P. U. Diaz, F. B. Gasser, M. M. L. Velazquez, N. C. Gareis, N. R. Salvetti, H. H. Ortega, M. E. Baravalle
Summary: Cystic ovarian disease (COD) is a significant cause of reproductive failure in dairy cattle. This review focuses on the role of inflammation and angiogenesis in the development of follicular cysts, particularly in bovine species. The study suggests that changes in the expression of cytokines and angiogenic factors contribute to the abnormal persistence of follicular cysts. Pro-inflammatory and anti-inflammatory cytokines play a crucial role in regulating angiogenesis, particularly in ovarian cells from follicular cysts and persistent follicles. The imbalance in cytokine expression and pro-angiogenic growth factors may contribute to follicular persistence and the recurrent appearance of COD in dairy cattle.
Article
Developmental Biology
Fernanda M. Rodriguez, Ulises S. Notaro, Emilia Huber, Sebastian Recce, Hugo H. Ortega, Marcelo L. Signorini, Florencia Rey, Natalia R. Salvetti
Summary: This study evaluated the impact of heat stress during gestation on molecular aspects at the ovarian level in adult offspring. The results showed associations between heat stress variables during gestation and the methylation status of CpG sites in the 5'UTR of ESRI and ESR2, as well as the expression of ESRI, ESR2, PGR, and coregulatory proteins in dominant follicles of adult cows.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Oncology
Georgina A. Cardama, Julian Maggio, Lucas Valdez Capuccino, Nazareno Gonzalez, Valentina Matiller, Hugo H. Ortega, German R. Perez, Ignacio A. Demarco, Eduardo Spitzer, Daniel E. Gomez, Pablo Lorenzano Menna, Daniel F. Alonso
Summary: Malignant gliomas are the most common primary central nervous system tumors in adults and are associated with poor prognosis. This study evaluates the potential of Rac1 inhibitor 1A-116 as a targeted therapy for glioma and provides evidence of its efficacy in both in vitro and in vivo models. These findings suggest 1A-116 as a promising treatment option for glioma.
Article
Biophysics
Joana B. Ferrado, Adrian A. Perez, Malen Menegon, Victoria A. Vaillard, Fatima Gasser, Maria E. Baravalle, Hugo H. Ortega, Santiago E. Vaillard, Liliana G. Santiago
Summary: In this study, self-assembled bovine serum albumin nanoparticles loaded with genistein were PEGylated to avoid possible immunogenic reactions and rapid clearance from the bloodstream. The PEGylation process increased the particle size of the nanoparticles and improved their biological properties, reducing cytotoxicity and genotoxicity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Pharmacology & Pharmacy
Maria Laura Soriano Perez, Javier Alejandro Funes, Carolina Flores Bracamonte, Luis Exequiel Ibarra, Marina Andrea Forrellad, Oscar Taboga, Laura Noelia Cariddi, Facundo Jose Salinas, Hugo Hector Ortega, Fabrisio Alustiza, Maria Molina
Summary: This study reported the synthesis and performance of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM). The cytotoxicity, internalization ability, antigen protection and delivery capability of the nanogels were evaluated in vitro and in vivo. The results demonstrated that the pNIPAM nanogels can stimulate a humoral immune response.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Veterinary Sciences
Emmanuel Angeli, Daiana Barcarolo, Lucas E. Ribas, Valentina Matiller, Silvina M. Addona, Florencia Rey, Hugo H. Ortega, Gustavo J. Hein
Summary: This study aimed to analyze the possible relationship between days to conception and oxidative stress biomarkers and liver functional parameters in dairy cows. A reliable method for the accurate measurement of malondialdehyde (MDA) was developed. The results showed that there were no differences in systemic concentration of certain biomarkers between high and low days to conception groups. However, cholesterol concentration, plasma 3-nitrotyrosine (3-NT) concentration, and superoxide dismutase activity were found to be significantly different between the two groups. Lower levels of 3-NT and MDA in the liver were associated with better reproductive performance in dairy cows.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Daiana Barcarolo, Emmanuel Angeli, Lucas Etchevers, Lucas E. Ribas, Valentina Matiller, Florencia Rey, Hugo H. Ortega, Gustavo J. Hein
Summary: This study aimed to investigate the effects of parenteral vitamin and mineral supplementation on hepatic fatty acid metabolism and oxidative stress biomarkers in transition cows. The supplemented group received injections of vitamin A palmitate, vitamin E acetate, copper edetate, zinc edetate, manganese edetate, and sodium selenite, while the control group received injections of sodium chloride. Results showed that supplemented animals had higher GSH-Px activity, lower and higher concentration of 3-NT in the liver and plasma, respectively, higher expression of carnitine palmitoyltransferase 1, and lower triacylglycerol content in the liver. No differences were found in other parameters between groups. These findings suggest that vitamin and mineral supplementation has beneficial effects on GSH-Px activity, hepatic 3-NT concentration, and metabolic adaptation in transition cows.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Reproductive Biology
Ulises S. Notaro, Emilia Huber, Antonela F. Stassi, Nadia E. Ormaechea, Juan A. Chiaraviglio, M. Eugenia Baravalle, Hugo H. Ortega, Florencia Rey, Natalia R. Salvetti
Summary: The study aimed to evaluate the expression of estrogen receptors and coregulatory proteins in follicular persistence, a major component of cystic ovarian disease in dairy cattle. The results provide important information about the development of follicular persistence and potential therapeutic targets.
Article
Agriculture, Dairy & Animal Science
Camila Beccaria, Celina Baravalle, Paula Silvestrini, Maria S. Renna, Ana I. Molineri, Marcelo L. Signorini, Veronica E. Neder, Guillermo A. Suarez Archilla, Luis F. Calvinho, Bibiana E. Dallard
Summary: The study found that adding PGe to dry cow therapy did not show any advantage over the use of dry cow therapy alone, and milk production and SCC in the ensuing lactation were not affected by the treatment.
JOURNAL OF DAIRY RESEARCH
(2021)