Article
Environmental Sciences
Bohyun Shin, Se Hee Hong, Sumin Seo, Cho Hee Jeong, Jiyu Kim, Eunbin Bae, Donghee Lee, Jung Hoon Shin, Minki Shim, Sang Beom Han, Dong-Kyu Lee
Summary: Recent studies have found that long-term accumulation and exposure to novel brominated flame retardants (NBFRs) have negative effects on human health, leading to metabolic abnormalities and disruption of energy metabolism in liver cells. Metabolomics study revealed that accumulation of HBB (one of the NBFRs) resulted in significant reduction of amino acids and other metabolites. Molecular docking analysis suggested that HBB exposure may disrupt the energy metabolism of hepatic cells. This study provides insights into the hidden toxicity of non-regulated NBFRs.
Article
Pharmacology & Pharmacy
Qing-Qing Yu, Heng Zhang, Shiyuan Zhao, Dadi Xie, Haibo Zhao, Weidong Chen, Min Pang, Baoqin Han, Pei Jiang
Summary: This study comprehensively analyzed the metabolite changes in a mouse model of irinotecan-induced intestinal mucositis, identifying 46 metabolites with significant changes in tissues and serum. The study highlighted key pathways related to these metabolites, offering insights into new biomarkers for diagnosis and treatment of intestinal mucositis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Endocrinology & Metabolism
Jun Kou, Chunyang He, Lin Cui, Zhengping Zhang, Wei Wang, Li Tan, Da Liu, Wei Zheng, Wei Gu, Ning Xia
Summary: The study on Chinese postmenopausal women with osteoporosis identified potential biomarkers related to metabolic pathway disorders, providing new insights for early diagnosis. The findings suggest that metabonomic analysis has great potential for application in postmenopausal women with osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medical Laboratory Technology
Yuyang Hou, Yuanping He, Jingjie Wang, Yanfen Zhou, Bo Ma, Xin Li, Liang Shi, Qi Zhang
Summary: By analyzing the metabolic profiles of four different types of asthenozoospermia, this study revealed abnormal changes in metabolites and metabolic pathways associated with the disease. The findings provide new clues and evidence for the etiology, diagnosis, and treatment of asthenozoospermia.
CLINICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
Shiyuan Zhao, Haitao Zhong, Chunmei Geng, Hongjia Xue, Changshui Wang, Wenxue Sun, Ruili Dang, Wenxiu Han, Pei Jiang
Summary: In this study, gas chromatography-mass spectrometry was used to investigate the toxic mechanisms of ACR in the main target tissues of rats, revealing 14 metabolic pathways related to amino acid, fatty acid, purine, and energy metabolism. The study suggests that the toxic mechanism of ACR may involve oxidative stress, inflammation, amino acid metabolism, and energy disorders.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Multidisciplinary
Parker Ladd Bremer, Gert Wohlgemuth, Oliver Fiehn
Summary: Metabolomics by GC/MS is a standardized and reliable platform for understanding small molecule biology. The West Coast Metabolomics Center at UC Davis has collated over 156,000 samples and 2000 studies into the BinBase database, which provides meaningful insight to biologists and helps standardize metabolomics studies. They have developed an easy-to-use query interface, BinDiscover, for intuitive hypothesis generation based on these metabolomic samples.
JOURNAL OF CHEMINFORMATICS
(2023)
Article
Pharmacology & Pharmacy
Yanru Zhu, Feng Wang, Jiatong Han, Yunli Zhao, Miao Yu, Mingyan Ma, Zhiguo Yu
Summary: This study investigated the preventive effect and mechanism of L-theanine on depression in juvenile rats. The results confirmed the preventive effect of L-theanine and identified potential biomarkers related to its treatment. Overall, L-theanine achieved significant preventive results on depression by regulating various aspects of the body, such as amino acids, lipids, and inflammation.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Chiara Maria Vitale, Arjen Lommen, Carolin Huber, Kevin Wagner, Borja Garlito Molina, Rosalie Nijssen, Elliott James Price, Marco Blokland, Frederike van Tricht, Hans G. J. Mol, Martin Krauss, Laurent Debrauwer, Olga Pardo, Nuria Leon, Jana Klanova, Jean-Philippe Antignac
Summary: A set of QA/QC criteria for nontargeted measurement of pesticide exposure markers in human urine has been proposed and applied in the HBM4EU project. The criteria include monitoring signal intensities, mass accuracy, and retention times of selected markers. The results show high repeatability and accuracy of the data. This is the first time that harmonized QA/QC criteria have been used to assess the quality of nontargeted analysis of human samples across multiple laboratories.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Teresa S. McMillen, Andrew Leslie, Kenneth Chisholm, Susanne Penny, Jeffrey Gallant, Alejandro Cohen, Arik Drucker, James P. Fawcett, Devanand M. Pinto
Summary: This study presents a comprehensive and quantitatively robust method for metabolomic analysis, using HILIC and RP stationary phases combined with positive and negative electrospray ionization. The method allows for complete analysis of a single sample in as little as 40 minutes and has been validated with clinical samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Agriculture, Dairy & Animal Science
Jieyi Qin, Qingqing Cheng, Ziyu Cai, Lin Zhang, Tong Xing, Xinglian Xu, Feng Gao
Summary: The aim of this study was to identify biomarkers for the diagnosis of wooden breast (WB) myopathy in male broilers by characterizing the serum metabolic profiles using metabolomics analysis. A clear separation was observed between normal (CON) and WB groups based on the analysis of 73 significantly different metabolites. Nine significantly altered metabolites were identified as excellent discriminant biomarkers for WB myopathy. This study provides new insights into the pathogenesis of WB and offers potential biomarkers for diagnostic purposes.
Article
Medicine, General & Internal
Stefano Angioni, Francesca Congiu, Salvatore Giovanni Vitale, Maurizio Nicola D'Alterio, Antonio Noto, Giovanni Monni, Maria Laura Santoru, Vassilios Fanos, Federica Murgia, Luigi Atzori
Summary: This study used gas chromatography-mass spectrometer (GC-MS) to analyze serum samples of patients with symptomatic endometriosis and found metabolic alterations and potential biomarkers.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Environmental Sciences
Shiyuan Zhao, Jinxiu Guo, Hongjia Xue, Junjun Meng, Dadi Xie, Xi Liu, Qingqing Yu, Haitao Zhong, Pei Jiang
Summary: This study used gas chromatography-mass spectrometry to investigate the effects of fluoride exposure on metabolites in different organs. Potential biomarkers and affected metabolic pathways related to the mechanism of fluorosis were identified.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemical Research Methods
Shaobo Yang, Hong Yang, Yanlei Huang, Gong Chen, Chun Shen, Shan Zheng
Summary: Hirschsprung's disease (HSCR) is a congenital digestive tract malformation characterized by the absence of intramural ganglion cells in the myenteric and submucosal plexuses along variable lengths of the gastrointestinal tract. This study used metabolomic profiling to analyze HSCR serum samples and identified 21 biomarkers related to HSCR. It revealed that tryptophan metabolism was a crucial disordered pathway in HSCR. This is the first serum metabolomics study focusing on HSCR and provides a new perspective for explaining its mechanism.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Food Science & Technology
Shuo Mu, Nan Ni, Yuting Zhu, Sanne Boesveldt, Markus Stieger
Summary: Foods differing in fat content can be distinguished through olfaction alone. This study explores the mechanism behind humans' ability to discriminate and rank fat content in foods through olfaction. The volatile compound composition of beef and pork samples differing in fat content was analyzed, and certain compounds were found to be responsible for the olfactory discrimination of fat content. It was concluded that the volatile compounds in the headspace contribute to the discrimination of fat content based on olfaction alone.
FOOD RESEARCH INTERNATIONAL
(2023)
Review
Materials Science, Biomaterials
Muhammad Wasim, Haq Nawaz Khan, Hina Ayesha, Fazli Rabbi Awan
Summary: Even in the post genomic era, some developing countries, including Pakistan, do not have national level newborn screening (NBS) programs for inborn errors of metabolism (IEMs). Lack of technical expertise, high-end omics based analytical facilities, and meager funding for healthcare are the main reasons for the unavailability of NBS programs. This overview emphasizes the need for NBS programs in developing countries like Pakistan, where a significant population and consanguinity rate exist, by highlighting the potential benefits for IEM patients and society.
Review
Biochemistry & Molecular Biology
Annamaria Landolfi, Carlo Ricciardi, Leandro Donisi, Giuseppe Cesarelli, Jacopo Troisi, Carmine Vitale, Paolo Barone, Marianna Amboni
Summary: Diagnosis and treatment of Parkinson's disease is a challenging issue, with machine learning algorithms being widely applied and developed for the diagnosis and characterization of Parkinson's disease, helping to find common patterns from large amounts of data.
CURRENT MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Jacopo Troisi, Giorgia Venutolo, Concetta Terracciano, Matteo Delli Carri, Simone Di Micco, Annamaria Landolfi, Alessio Fasano
Summary: Intercellular tight junctions play a role in various chronic and acute inflammatory diseases, with Larazotide, a zonulin antagonist, potentially serving as a therapeutic strategy. Research has shown the efficacy of Larazotide in different diseases, indicating its potential as a treatment option.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Genetics & Heredity
Jacopo Troisi, Pierpaolo Cavallo, Sean Richards, Steven Symes, Angelo Colucci, Laura Sarno, Annamaria Landolfi, Giovanni Scala, David Adair, Carla Ciccone, Giuseppe M. Maruotti, Pasquale Martinelli, Maurizio Guida
Summary: Metabolomic profiling of maternal serum was used to identify a unique fingerprint for fetal heart anomalies, resulting in accurate classification models and the discovery of key metabolites. This approach shows promise for sensitive and effective screening of congenital defects, providing insights into the underlying pathophysiology of affected pregnancies.
PRENATAL DIAGNOSIS
(2021)
Article
Nutrition & Dietetics
Alessio Maria Monteleone, Jacopo Troisi, Gloria Serena, Alessio Fasano, Riccardo Dalle Grave, Giammarco Cascino, Francesca Marciello, Simona Calugi, Giovanni Scala, Giulio Corrivetti, Palmiero Monteleone
Summary: Alterations in the gut microbiome and fecal metabolites were found in anorexia nervosa (AN), with differences seen between those with restricting AN (ANR) and binge-purging AN (ANBP) types. ANBP patients showed significant differences in microbial abundances and metabolites compared to ANR patients. Despite small sample size, this study confirms the occurrence of distinct gut dysbiosis in ANR and ANBP, showcasing different connections between gut microorganisms and fecal metabolites in the two AN types.
Article
Chemistry, Multidisciplinary
Jacopo Troisi, Angelo Colucci, Pierpaolo Cavallo, Sean Richards, Steven Symes, Annamaria Landolfi, Giovanni Scala, Francesco Maiorino, Alfonso Califano, Marco Fabiano, Gianmarco Silvestre, Federica Mastella, Alessandro Caputo, Antonio D'Antonio, Vincenzo Altieri
Summary: The study presents a serum metabolomics signature of bladder cancer along with a robust ensemble machine learning algorithm for effective patient discrimination. The signature shows potential as a reliable screening test if validated on a larger cohort, and it is able to discriminate high- and low-grade cancers. These findings contribute to the clinical understanding and early detection of bladder cancer, which has high morbidity and mortality.
APPLIED SCIENCES-BASEL
(2021)
Review
Nutrition & Dietetics
Martina Lombardi, Jacopo Troisi
Summary: Autism is a neurodevelopmental disorder characterized by genetic and environmental factors, with gut dysbiosis possibly contributing to autism symptoms. Current studies on microbial-based and dietary interventions lack consistency and comparability, highlighting the need for standardized methods in future research.
Article
Biochemistry & Molecular Biology
Jacopo Troisi, Maria Tafuro, Martina Lombardi, Giovanni Scala, Sean M. Richards, Steven J. K. Symes, Paolo Antonio Ascierto, Paolo Delrio, Fabiana Tatangelo, Carlo Buonerba, Biancamaria Pierri, Pellegrino Cerino
Summary: The objective of this study was to develop a novel screening approach for colorectal cancer using serum metabolomic profiles, and to evaluate the metabolic alterations associated with the disease. The results showed that the metabolomic signature can be used as a non-invasive screening tool for colorectal cancer, and identified differences primarily associated with cell glucose metabolism.
Article
Food Science & Technology
Paola Pontieri, Jacopo Troisi, Matteo Calcagnile, Scott R. Bean, Michael Tilley, Fadi Aramouni, Antonio Boffa, Giacomo Pepe, Pietro Campiglia, Fabio Del Giudice, Alberto L. Chessa, Dmitriy Smolensky, Mariarosaria Aletta, Pietro Alifano, Luigi Del Giudice
Summary: Grain sorghum is a gluten-free cereal with different pericarp colors, and the different colors of sorghum have unique nutritional properties. Red and black sorghum varieties have higher fiber content, while white sorghum variety has higher saturated fat content. There are also differences in mineral content among different pericarp colors of sorghum.
Article
Medicine, General & Internal
Armando Ugo Cavallo, Jacopo Troisi, Emanuele Muscogiuri, Pierpaolo Cavallo, Sanjay Rajagopalan, Rodolfo Citro, Eduardo Bossone, Niall McVeigh, Valerio Forte, Carlo Di Donna, Francesco Giannini, Roberto Floris, Francesco Garaci, Massimiliano Sperandio
Summary: The aim of this study is to verify the feasibility of a radiomics based approach for the detection of LV remodeling in patients with arterial hypertension. The study collected CCT images and clinical data of patients with and without hypertension, and applied radiomics and machine learning to classify and detect LV remodeling. The results showed that the radiomics approach was able to accurately identify LV remodeling in patients with past history of arterial hypertension.
Article
Clinical Neurology
Claudia Vinciguerra, Antonella Toriello, Valerio Nardone, Daniele Romano, Salvatore Tartaglione, Filomena Abate, Annamaria Landolfi, Paolo Barone
Summary: Temporal muscle thickness (TMT) has shown potential prognostic relevance in patients with Amyotrophic Lateral Sclerosis (ALS), correlating with functional rating and compound motor action potential. TMT may serve as a promising surrogate biomarker for survival and functional status in ALS.
NEUROLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jacopo Troisi, Antonio Mollo, Martina Lombardi, Giovanni Scala, Sean M. Richards, Steven J. K. Symes, Antonio Travaglino, Daniele Neola, Umberto de Laurentiis, Luigi Insabato, Attilio Di Spiezio Sardo, Antonio Raffone, Maurizio Guida
Summary: Endometrial cancer is the most common gynecological neoplasm in high-income countries, but no validated screening tests are currently available. This study validated a metabolomics signature as an effective screening test for symptomatic women, demonstrating that serum metabolome can serve as a cost-effective, non-invasive, and accurate EC screening test.
Article
Clinical Neurology
Annamaria Landolfi, Claudia Vinciguerra, Francesco Diana, Filomena Murano, Maria Claudia Russillo, Paolo Barone, Bianca Serio, Giuseppe Piscosquito
Summary: We present a case of intrathecal methotrexate toxicity in a patient with acute lymphoblastic leukemia and summarize existing literature on this topic. The patient developed flaccid paraparesis, which improved with treatment. The literature review confirms the selective involvement of lumbosacral ventral roots in intrathecal methotrexate toxicity, possibly due to localized folate deficiency or immune-mediated mechanisms.
NEUROLOGICAL SCIENCES
(2023)
Article
Clinical Neurology
Annamaria Landolfi, Marina Picillo, Maria Teresa Pellecchia, Jacopo Troisi, Marianna Amboni, Paolo Barone, Roberto Erro
Summary: This study aims to provide a briefer version of the Italian-adapted UPSIT test that can discriminate between Parkinson's disease patients and healthy subjects. By using statistical approaches and machine learning, 8 items were selected and tested, showing better performance compared to the 40-item version. The selected 8 odors demonstrated higher sensitivity and specificity in predicting the PD condition.
NEUROLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jacopo Troisi, Steven J. K. Symes, Martina Lombardi, Pierpaolo Cavallo, Angelo Colucci, Giovanni Scala, David C. Adair, Maurizio Guida, Sean M. Richards
Summary: Fetal growth restriction (FGR) is a condition that leads to high neonatal mortality and morbidity. The mechanisms behind FGR are not fully understood. In this study, metabolites were extracted from placentas of 493 non-complicated pregnancies using gas chromatography-mass spectrometry (GC-MS). The results showed that certain metabolites were significantly different in placentas of low birth weight (LBW) and small for gestational age (SGA) newborns compared to non-LBW and adequate for gestational age (AGA) newborns. These findings indicate that metabolic pathways related to hypoxia response, amino acid uptake, and inflammation play a role in FGR.
Meeting Abstract
Clinical Neurology
Annamaria Landolfi, Bianca Serio, Filomena Murano, Paolo Barone, Claudia Vinciguerra, Giuseppe Piscosquito
JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM
(2022)