Article
Agricultural Engineering
Tayyab Islam, Thuan Phu Nguyen-Vo, Vivek Kumar Gaur, Junhak Lee, Sunghoon Park
Summary: The production of 1,3-butanediol from glucose was achieved by engineering a pathway in Escherichia coli and optimizing gene expression levels and culture conditions. The highest ever reported titer of 1,3-butanediol in biological production methods was achieved at 257 mM in 36 hours with a yield of 0.51 mol/mol. This study provides a promising strategy for sustainable synthesis of other acetyl-CoA-derived chemicals using microbial cell factories.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Tayyab Islam, Thuan Phu Nguyen-Vo, Seunghyun Cho, Junhak Lee, Vivek Kumar Gaur, Sunghoon Park
Summary: This study focuses on metabolic engineering of Escherichia coli to efficiently produce 1,3-BDO. By reducing the accumulation of intermediate metabolites and byproducts and improving NADPH availability, a record-high titer of optically pure (R)-1,3-BDO was achieved.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: Efforts to improve the production efficiency of (R)-1,3-BDO by Escherichia coli through different metabolic engineering strategies have successfully optimized key pathway enzymes, increased NADPH supply, optimized fermentation conditions, and reduced byproducts formation. The best engineered strain can efficiently produce (R)-1,3-BDO with a yield of 0.6 mol/mol glucose, corresponding to 60% of the theoretical yield. Additionally, the feasibility of aerobically producing 1,3-BDO via a new pathway using 3-hydroxybutyrate as an intermediate has been demonstrated.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Cell Biology
Wensi Meng, Lijie Zhang, Menghao Cao, Yongjia Zhang, Yipeng Zhang, Ping Li, Zhaoqi Kang, Shiting Guo, Ping Xu, Cuiqing Ma, Chao Gao
Summary: Research demonstrates that acetate accumulation in certain bacterial growth processes exhibits an initial increase followed by a decrease. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and crucial for the elimination of toxic acetate. This unique overflow metabolism with desirable metabolic functions is important for industrial applications of various bacteria.
Article
Biotechnology & Applied Microbiology
Lili Sheng, Abubakar Madika, Matthew S. H. Lau, Ying Zhang, Nigel P. Minton
Summary: This study engineered the thermophile Parageobacillus thermoglucosidasius NCIMB 11955 to produce 3-hydroxybutanone and 2,3-butanediol, organic compounds with commercial applications. By minimizing by-product formation and addressing redox imbalance, the production of targeted metabolites was significantly improved. The highest titre of 2,3-butanediol produced in Parageobacillus and Geobacillus species to date, 15.6 g/L, was achieved in media supplemented with 5% glucose.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Sofia Maina, Ashish A. Prabhu, Narisetty Vivek, Anestis Vlysidis, Apostolis Koutinas, Vinod Kumar
Summary: This review summarizes the applications of bio-based platform chemicals 2,3-butanediol (BDO) and acetoin in various industries, as well as the novel technological developments in genetic and metabolic engineering strategies for their production. The review also discusses the valorization of renewable feedstocks and the development of bioprocesses for the production of BDO and acetoin. Techno-economic aspects evaluating the viability and industrial potential of bio-based BDO production are presented.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Biotechnology & Applied Microbiology
Yuchen Li, Xiangying Zhao, Mingjing Yao, Wenli Yang, Yanlei Han, Liping Liu, Jiaxiang Zhang, Jianjun Liu
Summary: This paper reviews the synthesis pathways and formation mechanisms of different stereoisomers of 3-hydroxybutanone (acetoin) and 2,3-butanediol in microorganisms. It also summarizes the characteristics and affinities of different types of butanediol dehydrogenases (BDH) and discusses the recent research on producing optically pure acetoin or 2,3-butanediol using microorganisms. The limiting factors and possible solutions for chiral acetoin and 2,3-butanediol production are also discussed.
MICROBIAL CELL FACTORIES
(2023)
Article
Agricultural Engineering
Daisuke Nonaka, Ryosuke Fujiwara, Yuuki Hirata, Tsutomu Tanaka, Akihiko Kondo
Summary: The study demonstrated an enhanced production efficiency of 1,2-propanediol using metabolically engineered Escherichia coli, with successful synthesis from glucose and cellobiose. Improved production was achieved by deleting competing pathways and inactivating the PTS system to reduce carbon flux towards downstream glycolysis.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Jie Cheng, Juan Li, Linggang Zheng
Summary: 1,4-Butanediol is a significant commodity chemical currently produced through energy-intensive processes with severe environmental impacts. As market demand increases, attention has shifted towards sustainable bioproduction methods, leading to advancements in metabolic engineering strategies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Microbiology
Mengyang Lei, Zetian Qiu, Leilei Guan, Zheng Xiang, Guang-Rong Zhao
Summary: In this study, a metabolic engineering approach was employed to produce Z,Z-farnesol (Z,Z-FOH) in Escherichia coli. By testing various enzymes and mutants, the optimal strain was able to achieve the highest reported titer of Z,Z-FOH in microbes. This work not only provides insights into the de novo biosynthesis of Z,Z-FOH in E. coli, but also lays a foundation for the development of synthetic E. coli cell factories for other cis-configuration terpenoids.
Article
Biotechnology & Applied Microbiology
Kaloyan Petrov, Penka Petrova
Summary: The review discusses the advantages of Bacillus spp. as potential producers of acetoin and 2,3-BD, including their non-pathogenic nature, adaptability to various substrates, and the potential for genetic engineering and process optimization. While Bacillus spp. are among the best acetoin producers, they also synthesize 2,3-BD comparable to pathogenic producers but with relatively lower productivity, which could be improved through future research.
FERMENTATION-BASEL
(2021)
Article
Microbiology
Parsa Ghadermazi, Angela Re, Luca Ricci, Siu Hung Joshua Chan
Summary: Gas fermentation is a promising method to convert low-cost waste gases into valuable chemicals. This study used modeling and kinetic ensemble modeling to analyze metabolic engineering interventions for increased 2,3-butanediol production and reduced carbon loss. The results suggested that interventions targeting by-products branching from acetyl-CoA and pyruvate are effective strategies.
Article
Biotechnology & Applied Microbiology
Weiwei Bao, Wei Shen, Qiqun Peng, Jun Du, Shihui Yang
Summary: Biorefinery for producing value-added biochemicals is a promising alternative for meeting sustainable energy and environmental goals. This study applied metabolic engineering strategies to enhance acetoin production in Zymomonas mobilis by introducing a heterologous acetoin pathway and manipulating NADH levels. The recombinant strain achieved significantly improved acetoin production.
FERMENTATION-BASEL
(2023)
Article
Agricultural Engineering
Sofia Maina, Roland Schneider, Maria Alexandri, Harris Papapostolou, George-John Nychas, Apostolis Koutinas, Joachim Venus
Summary: The production of acetoin and D-BDO by Bacillus amyloliquefaciens cultivated on bakery waste hydrolysates can be influenced by varying the volumetric oxygen transfer coefficient (kLa). Batch fermentations with different kLa values were found to have significant impact on the production yields and rates, with fed-batch cultures at higher kLa values leading to combined production of acetoin, meso-BDO and D-BDO. The results suggest that manipulating the kLa value can redirect bacterial metabolism towards higher acetoin or D-BDO production during fermentation in crude bakery waste hydrolysates.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Seon-Hwa Lee, Soo-Jin Yeom, Seong-Eun Kim, Deok-Kun Oh
Summary: Aldol chemicals are synthesized by condensation reactions between ketones and aldehydes using aldolases. Improving the catalytic properties of aldolases through engineering and design, as well as combining them with other catalysts, enables the efficient synthesis of complex organic chemicals. Current challenges and emerging solutions for aldolase-based catalysts are also discussed.
TRENDS IN BIOTECHNOLOGY
(2022)
Review
Biochemical Research Methods
Jee In Yoo, Yu Jung Sohn, Jina Son, Seo Young Jo, Jiwon Pyo, Su Kyeong Park, Jong-Il Choi, Jeong Chan Joo, Hee Taek Kim, Si Jae Park
Summary: This article discusses the global environmental issues caused by heavy reliance on petroleum-based industries and the shift towards biorefineries using natural and engineered microorganisms. The focus is on the production of versatile C4 alcohols using microbial cell factories and metabolic engineering strategies for potential commercialization.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Physical
Dong Hyun Kim, Dong Oh Han, Kyu In Shim, Jae Kyun Kim, Jeffrey G. Pelton, Mi Hee Ryu, Jeong Chan Joo, Jeong Woo Han, Hee Taek Kim, Kyoung Heon Kim
Summary: PET, widely used in various industries, poses major environmental challenges due to its low degradability and recycling rate. An integrated process for depolymerizing PET and converting it to high-value products has been developed, showing great potential for upcycling waste PET.
Article
Chemistry, Multidisciplinary
Hee Taek Kim, Mi Hee Ryu, Ye Jean Jung, Sooyoung Lim, Hye Min Song, Jeyoung Park, Sung Yeon Hwang, Hoe-Suk Lee, Young Joo Yeon, Bong Hyun Sung, Uwe T. Bornscheuer, Si Jae Park, Jeong Chan Joo, Dongyeop X. Oh
Summary: This study developed a chemo-biological upcycling strategy for poly(ethylene terephthalate) waste, involving PET depolymerization, biotransformation of TPA into catechol, and application as a coating agent. The process demonstrated efficient conversion of PET waste into multifunctional coating materials without the need for extensive purification or evaporation.
Review
Chemistry, Multidisciplinary
Kei-Anne Baritugo, Jina Son, Yu Jung Sohn, Hee Taek Kim, Jeong Chan Joo, Jong-il Choi, Si Jae Park
Summary: This study discusses the recent development of Corynebacterium glutamicum in producing platform chemicals from renewable resources, utilizing synthetic biology tools and techniques to transform it into a versatile microbial cell factory.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Sion Ham, Yeong-Hoon Han, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Hong-Ju Lee, See-Hyoung Park, Kyungmoon Park, Jung-Oh Ahn, Jeong Chan Joo, Shashi Kant Bhatia, Yung-Hun Yang
Summary: In this study, the use of GOX from Streptomyces sp. X119-6 and KatE from Escherichia coli in a whole-cell system significantly increased the production of alpha-KG. However, severe substrate inhibition was observed at high substrate concentrations.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Seo Young Jo, Yu Jung Sohn, Se Young Park, Jina Son, Jee In Yoo, Kei-Anne Baritugo, Yokimiko David, Kyoung Hee Kang, Hoyong Kim, Jong-il Choi, Mi Na Rhie, Hee Taek Kim, Jeong Chan Joo, Si Jae Park
Summary: The study demonstrated that pretreating sugarcane molasses with activated charcoal can enhance the growth and yield of Ralstonia eutropha strains expressing the Manheimia succiniciproducens sacC gene in the culture medium. Additionally, co-expression of the ldhA gene and sacC gene in Ralstonia eutropha strains allows for the production of P(3HB-co-LA) from sugarcane molasses.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Agricultural Engineering
Kyoungseon Min, Hee Taek Kim, Si Jae Park, Siseon Lee, Ye Jean Jung, Jin-Suk Lee, Young Je Yoo, Jeong Chan Joo
Summary: This study explores a structure-based strategy to stabilize lipase A from Bacillus subtilis in a water-ethanol cosolvent, resulting in 4 mutants with higher stability in ethanol and potential applications in enzyme-based biorefineries for producing value-added fuels and chemicals.
BIORESOURCE TECHNOLOGY
(2021)
Review
Agricultural Engineering
Jina Son, Kei-Anne Baritugo, Seo Hyun Lim, Hye Jin Lim, Seona Jeong, Ji Yeon Lee, Jong-il Choi, Jeong Chan Joo, Jeong-Geol Na, Si Jae Park
Summary: Currently, linear petroleum-based industries are causing depletion of finite natural resources and accumulation of non-biodegradable and hazardous wastes, highlighting the need for ecofriendly and sustainable solutions. Utilizing agro-industrial wastes as substrates can lead to the production of target value-added chemicals like organic acids, with advancements in metabolic engineering strategies and techniques enhancing their production. Further development and future perspectives in utilizing waste-based biorefineries for organic acid production from various industries have been discussed to showcase their importance.
BIORESOURCE TECHNOLOGY
(2022)
Review
Agricultural Engineering
Yu Jung Sohn, Jina Son, Seo Young Jo, Se Young Park, Jee In Yoo, Kei-Anne Baritugo, Jeong Geol Na, Jong-il Choi, Hee Taek Kim, Jeong Chan Joo, Si Jae Park
Summary: Cupriavidus necator is a versatile microorganism capable of accumulating large amounts of PHAs from renewable resources. Through metabolic engineering, it has the potential for applications in synthesizing biopolymers, biofuels, and biochemicals.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yu Jung Sohn, Minsoo Kang, Mi-Hee Ryu, Siseon Lee, Kyoung Hee Kang, Yunjae Hong, Bong Keun Song, Kyungmoon Park, Si Jae Park, Jeong Chan Joo, Hee Taek Kim
Summary: Corynebacterium glutamicum was engineered to produce glutaric acid using metabolic engineering. Through batch fermentation and pilot-scale fermentation, a large amount of glutaric acid was successfully produced. The glutaric acid was then used to synthesize dioctyl glutarate, which showed comparable effects in PVC film and leather coating to other plasticizers.
Review
Agricultural Engineering
Jina Son, Jeong Chan Joo, Kei-Anne Baritugo, Seona Jeong, Ji Yeon Lee, Hye Jin Lim, Seo Hyun Lim, Jee In Yoo, Si Jae Park
Summary: Traditional organic acid production relies heavily on petroleum-based industries, which are now facing obsolescence due to the inability to achieve a virtuous cycle of carbon release. Therefore, the development of efficient microbial cell factories using renewable feedstocks offers a promising transition towards a circular economy in terms of carbon footprint.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Siseon Lee, Ye Jean Jung, Si Jae Park, Mi-Hee Ryu, Joo Eon Kim, Hye Min Song, Kyoung Hee Kang, Bong Keun Song, Bong Hyun Sung, Yong Hwan Kim, Hee Taek Kim, Jeong Chan Joo
Summary: An engineered Pseudomonas putida strain was developed to produce 2-pyrone-4,6dicarboxylic acid (PDC) from lignin-derived monomeric compounds. The biosynthetic pathway for PDC production was established and fermentation of the final strain resulted in 22.7 g/L PDC.
BIORESOURCE TECHNOLOGY
(2022)
Review
Green & Sustainable Science & Technology
Gwon Woo Park, Gyeongtaek Gong, Jeong Chan Joo, Jinju Song, Jiye Lee, Joon-Pyo Lee, Hee Taek Kim, Mi Hee Ryu, Ranjna Sirohi, Xinshu Zhuang, Kyoungseon Min
Summary: This article provides a comprehensive review on biological approaches, such as enzymes and microorganisms, for lignin degradation. It also highlights recent advancements in biotechnological lignin valorization, including metabolic engineering for producing useful fuels and chemicals and the use of lignin-based hybrid materials for biosensor and biomedical applications. Future research directions and the potential of utilizing all components of lignocellulosic biomass in zero-waste refineries are discussed.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Jina Son, Kei-Anne Baritugo, Yu Jung Sohn, Kyoung Hee Kang, Hee Taek Kim, Jeong Chan Joo, Si Jae Park
Summary: In this study, recombinant strains of Corynebacterium glutamicum were developed to express mutated GAD from Escherichia coli, GADs from Lactococcus lactis CICC20209, and Lactobacillus senmaizukei. These strains showed enhanced stability and adaptability at a pH of approximately 7.0. Batch fermentation experiments showed improved GABA production and yields at pH 6.0 and pH 7.0 from 100 g/L glucose.
Article
Biotechnology & Applied Microbiology
Haeng-Geun Cha, Hee Taek Kim, See-Hyoung Park, Kyoungseon Min, Youjung Kong, Jeong Chan Joo, Yung-Hun Yang, Jung-Oh Ahn, Jin-Suk Lee, Kyungmoon Park
Summary: A system using monosodium glutamate (MSG) as an alternative to expensive alpha-ketoglutaric acid (alpha-KG) was developed for the bioproduction of glutaric acid through the whole-cell conversion reaction catalyzed by L-glutamate oxidase (Gox). The glutaric acid production was significantly increased by applying the stepwise feeding strategy of MSG.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)