Article
Biochemistry & Molecular Biology
Pedro Monteiro, Bongwhan Yeon, Samuel S. Wallis, Susana A. Godinho
Summary: Intracellular organelle organization in eukaryotic cells is achieved by active transport along microtubules. Centrosome amplification, commonly observed in cancer, induces a change in organelle positioning that facilitates nuclear migration. Increased tubulin acetylation differentially regulates kinesin-1-mediated organelle displacement to regulate intracellular organization.
Article
Cell Biology
Klara I. Jansen, Malina K. Iwanski, Mithila Burute, Lukas C. Kapitein
Summary: Jansen et al. have developed a live-cell marker called StableMARK, which allows for visualization of stable microtubules. This marker facilitates the study of different subsets of microtubules throughout the cell cycle and their role in cellular organization and transport.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Immunology
Victoria Lucia Alonso, Mara Emilia Carloni, Camila Silva Goncalves, Gonzalo Martinez Peralta, Maria Eugenia Chesta, Alejandro Pezza, Luis Emilio Tavernelli, Maria Cristina M. Motta, Esteban Serra
Summary: Trypanosomatids have a simpler cytoskeleton arrangement with stable microtubules, extensively acetylated in Trypanosoma cruzi. The identification of T. cruzi ATAT as a tubulin acetyltransferase sheds light on the role of alpha-tubulin acetylation in regulating cell cycle progression and parasite biology. Over-expression of TcATAT leads to morphological defects, kinetoplast division impairment, and increased resistance to microtubule depolymerizing drugs in T. cruzi, emphasizing the importance of finely regulated alpha-tubulin acetylation levels for normal cell cycle progression.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Cell Biology
Ada Nowosad, Justine Creff, Pauline Jeannot, Raphael Culerrier, Patrice Codogno, Stephane Manenti, Laurent Nguyen, Arnaud Besson
Summary: In response to glucose starvation, p27 promotes autophagy by facilitating autophagosome trafficking along microtubule tracks via maintaining elevated microtubule acetylation through an ATAT1-dependent mechanism.
CELL DEATH & DISEASE
(2021)
Review
Cell Biology
Jaylyn Waddell, Aditi Banerjee, Tibor Kristian
Summary: Mitochondria are unique organelles that play multifunctional roles in cellular physiology and pathophysiology, with their dynamics regulated by specific proteins and post-translational modifications. The interplay between these regulatory mechanisms and intracellular cytoskeleton structures influences mitochondrial fusion and fission, contributing to physiological and pathological processes. Further understanding of this regulation may provide insights for potential therapeutic strategies in conditions like acute brain injury.
Review
Chemistry, Medicinal
Mauricio T. Tavares, Alan P. Kozikowski, Sida Shen
Summary: Histone deacetylase 6 (HDAC6) is a zinc-dependent enzyme that regulates the acetylation of non-histone substrates, impacting various cellular processes. The discovery of mercaptoacetamide (MCA)-based HDAC6 inhibitors has shown promising potential due to their selectivity and lack of mutagenicity. Further research on the structure-activity relationships, selectivity, and pharmacological properties of MCA-based HDAC6 inhibitors could lead to improved drug-like properties.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Jose Martinez-Hernandez, Julie Parato, Aditi Sharma, Jean-Marc Soleilhac, Xiaoyi Qu, Ellen Tein, Andrew Sproul, Annie Andrieux, Yves Goldberg, Marie-Jo Moutin, Francesca Bartolini, Leticia Peris
Summary: This study found an accumulation of tubulin acetylation in post-mortem brain tissues from Alzheimer's disease patients and human neurons with the Alzheimer's familial APP-V717I mutation. Impaired tubulin re-tyrosination may contribute to this acetylation accumulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Reproductive Biology
Song Gao, Zheng Wang, Jukui Ma, Jingyi Wang, Xupeng Xing, Chenglong Shen, Zhihan Niu, Heqiang Li, Shaowei Zhang, Kaijuan Zhang, Jing Gao, Yong Zhang, Yongsheng Wang
Summary: Recent studies have shown that abnormal cytoskeletal changes are an important cause of developmental failure in somatic cell nuclear transfer (SCNT) embryos, in addition to alterations in abnormal chromosome epigenetic modifications. In this study, the effects of ACY-1215, a specific inhibitor of HDAC6, on the acetylation of alpha-tubulin, histone epigenetic modification, spindle formation, and embryonic development of early bovine SCNT embryos were investigated. The results showed that ACY-1215 treatment improved the acetylation level of alpha-tubulin and histone proteins, corrected spindle abnormalities, delayed cleavage timing, increased cell numbers, and reduced apoptosis in SCNT blastocysts. ACY-1215 was found to regulate the process of embryonic epigenetic modification and cytoskeletal protein acetylation, leading to improved SCNT embryonic development potential.
Article
Biochemistry & Molecular Biology
Maria Ovejero-Sanchez, Gloria Asensio-Juarez, Myriam Gonzalez, Pilar Puebla, Miguel Vicente-Manzanares, Rafael Pelaez, Rogelio Gonzalez-Sarmiento, Ana Belen Herrero
Summary: This study synthesized and characterized a new microtubule-destabilizing agent, PILA9, which showed strong cytotoxicity against OC cells. Additionally, the combination of microtubule-destabilizing agents with Panobinostat synergistically enhanced cytotoxicity, potentially through inducing alpha-tubulin acetylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Rong Huang, Liyan Sui, Cong Fu, Yanhui Zhai, Xiangpeng Dai, Sheng Zhang, Ziyi Li
Summary: Inhibition of HDAC11 significantly affects meiosis by altering acetylation levels of key molecules, leading to disrupted maturation of porcine oocytes and compromised subsequent embryo development.
Article
Oncology
Jakeb S. S. M. Petersen, Sarah K. Baird
Summary: The study compared the efficacy of anthelmintic drugs mebendazole and albendazole in breast and colon cancer cell lines, revealing their selective cancer cell killing ability through classical apoptosis and cell cycle arrest involving microtubule destabilization.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Na Jiang, Binna Lv, Haixia Wu, Shidong Li, Manhong Sun
Summary: The study constructed a TMT-labelled lysine acetylome in Clonostachys chloroleuca 67-1, identifying a large number of Kac proteins with differential modification. Bioinformatics analysis showed that these Kac proteins are involved in diverse functions and have a broad regulatory effect.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Amy N. Sinclair, Christopher L. de Graffenried
Summary: A recent study on a highly divergent eukaryote revealed that unusual tubulin isoforms can create a mitotic spindle exclusively out of microtubule bundles, shedding light on a new mechanism of mitosis.
Article
Cell Biology
Xiaxia Niu, Ting Wu, Qishuang Yin, Xinsheng Gu, Gege Li, Changlong Zhou, Mei Ma, Li Su, Shu Tang, Yanan Tian, Ming Yang, Hongmei Cui
Summary: This study found that the PXR antagonist SPA70 can reduce resistance to paclitaxel in non-small cell lung cancer (NSCLC). The combination of PTX and SPA70 may be a potential novel treatment for taxane-resistant lung cancer.
Article
Cell Biology
Amir Mohammad Rahimi, Mingfang Cai, Irem Kilic, Zahra Basir Kazerouni, Constanza Tapia Contreras, Sigrid Hoyer-Fender
Summary: The study found that the ability of NIH3T3 fibroblasts to form cilia is related to the acetylation of cytoplasmic tubulin, mainly due to the reduced expression of alpha-tubulin acetyltransferase 1 (Atat1). Cells with decreased expression of Atat1 and tubulin acetylation proliferate faster, eventually gaining an advantage in cell competition.
Article
Biochemistry & Molecular Biology
Jun Negishi, Yuka Omori, Mami Shindo, Hayate Takanashi, Shiori Musha, Suminori Nagayama, Jun Hirayama, Hiroshi Nishina, Takashi Nakakura, Chihiro Mogi, Koichi Sato, Fumikazu Okajima, Yuta Mochimaru, Hideaki Tomura
JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION
(2017)
Article
Agriculture, Dairy & Animal Science
Kazuhiro Satou, Yuta Mochimaru, Takashi Nakakura, Tomoyuki Kusada, Jun Negishi, Shiori Musha, Nanaka Yoshimura, Yukio Kato, Hideaki Tomura
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2017)
Article
Multidisciplinary Sciences
Tomoaki Nakada, Fumiyo Toyoda, Kouhei Matsuda, Takashi Nakakura, Itaru Hasunuma, Kazutoshi Yamamoto, Satomi Onoue, Makoto Yokosuka, Sakae Kikuyama
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Kotaro Horiguchi, Ken Fujiwara, Saishu Yoshida, Takashi Nakakura, Ken Arae, Takehiro Tsukada, Rumi Hasegawa, Shu Takigami, Shunji Ohsako, Takashi Yashiro, Takako Kato, Yukio Kato
SCIENTIFIC REPORTS
(2018)
Article
Medicine, Research & Experimental
Toshiki Kurosawa, Yuma Tega, Kei Higuchi, Tomoko Yamaguchi, Takashi Nakakura, Tatsuki Mochizuki, Hiroyuki Kusuhara, Kenji Kawabata, Yoshiharu Deguchi
MOLECULAR PHARMACEUTICS
(2018)
Article
Biochemistry & Molecular Biology
Makiko Sato, Haruka Aoki-Saito, Hayato Fukuda, Hiroyuki Ikeda, Yasuhiko Koga, Masakiyo Yatomi, Hiroaki Tsurumaki, Toshitaka Maeno, Tsugumichi Saito, Takashi Nakakura, Tetsuya Mori, Masataka Yanagawa, Mitsuhiro Abe, Yasushi Sako, Kunio Dobashi, Tamotsu Ishizuka, Masanobu Yamada, Satoshi Shuto, Takeshi Hisada
Article
Biology
Saishu Yoshida, Katsuhiko Aoki, Ken Fujiwara, Takashi Nakakura, Akira Kawamura, Kohji Yamada, Masaya Ono, Satomi Yogosawa, Kiyotsugu Yoshida
Article
Cell Biology
Takashi Nakakura, Takeshi Suzuki, Hideyuki Tanaka, Kenjiro Arisawa, Toshio Miyashita, Yoko Nekooki-Machida, Toshiki Kurosawa, Yuma Tega, Yoshiharu Deguchi, Haruo Hagiwara
Summary: In this study, fenestrated endothelial cells were isolated from the rat pituitary and analyzed for gene expression levels related to ECM receptors using real-time PCR. Fibronectin was found to be the principal factor in maintaining sieve plate structures in fenestrated capillary endothelial cells, along with sustaining the expression pattern of specific endothelial marker genes.
CELL AND TISSUE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Takahiro Nemoto, Takashi Nakakura, Yoshihiko Kakinuma
Article
Cell Biology
Takashi Nakakura, Takeshi Suzuki, Kotaro Horiguchi, Hideyuki Tanaka, Kenjiro Arisawa, Toshio Miyashita, Yoko Nekooki-Machida, Haruo Hagiwara
Summary: This study investigated the role of fibronectin signaling in the formation of fenestrae and diaphragms in endothelial cells. It was found that fibronectin-integrin alpha 5 beta 1 signaling pathway regulates the microtubule cytoskeletons required for the transport of PLVAP proteins from the Golgi apparatus to sieve plates.
CELL AND TISSUE RESEARCH
(2021)
Article
Cell Biology
Takashi Nakakura, Hideyuki Tanaka, Takeshi Suzuki
Summary: The actin cytoskeleton plays a crucial role in the formation of endothelial fenestrae, inducing their formation and regulating cell arrangement. The interaction between dynamin2 and the actin cytoskeleton is essential for the control of fenestra formation in endothelial cells.
CELL AND TISSUE RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Kotaro Horiguchi, Saishu Yoshida, Takehiro Tsukada, Takashi Nakakura, Ken Fujiwara, Rumi Hasegawa, Shu Takigami, Shunji Ohsako
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2020)
Article
Cell Biology
Takashi Nakakura, Takeshi Suzuki, Seiji Torii, Anshin Asano-Hoshino, Yoko Nekooki-Machida, Hideyuki Tanaka, Kenjiro Arisawa, Yoshimi Nishijima, Takao Susa, Tomoki Okazaki, Yoshiko Kiuchi, Haruo Hagiwara
CELL AND TISSUE RESEARCH
(2017)
Article
Developmental Biology
Daisuke Kobayashi, Anshin Asano-Hoshino, Takashi Nakakura, Toshiyuki Nishimaki, Satoshi Ansai, Masato Kinoshita, Motoyuki Ogawa, Haruo Hagiwara, Takahiko Yokoyama
DEVELOPMENTAL BIOLOGY
(2017)