期刊
MASS SPECTROMETRY REVIEWS
卷 38, 期 1, 页码 79-111出版社
WILEY
DOI: 10.1002/mas.21574
关键词
binary interactomics; co-complex interactomics; interactomics technologies; protein-protein interactions
类别
资金
- Agentschap voor Innovatie door Wetenschap en Technologie
- FP7 Ideas
- European Research Council [340941]
The analysis of protein interaction networks is one of the key challenges in the study of biology. It connects genotypes to phenotypes, and disruption often leads to diseases. Hence, many technologies have been developed to study protein-protein interactions (PPIs) in a cellular context. The expansion of the PPI technology toolbox however complicates the selection of optimal approaches for diverse biological questions. This review gives an overview of the binary and co-complex technologies, with the former evaluating the interaction of two co-expressed genetically tagged proteins, and the latter only needing the expression of a single tagged protein or no tagged proteins at all. Mass spectrometry is crucial for some binary and all co-complex technologies. After the detailed description of the different technologies, the review compares their unique specifications, advantages, disadvantages, and applicability, while highlighting opportunities for further advancements.
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