期刊
MARINE DRUGS
卷 16, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/md16030095
关键词
spirotetronates; antimicrobial activity; Micromonospora sp.; structural elucidation
资金
- European Union's Seventh Framework Programme for research, technological development and demonstration [312184]
- Ministerio de Ciencia e Innovacion [PCT-010000-2010-4, INP-2011-0016-PCT-010000 ACT6, PCT-01000-ACT7]
- H2020 program [721421]
Phocoenamicins B and C (1 and 2), together with the known spirotetronate phocoenamicin (3), were isolated from cultures of Micromonospora sp. The acetone extract from a culture of this strain, isolated from marine sediments collected in the Canary Islands, displayed activity against methicillin-resistant Staphylococcus aureus (MRSA), Mycobacterium tuberculosis H37Ra and Mycobacterium bovis. Bioassay-guided fractionation of this extract using SP207ss column chromatography and preparative reversed-phased HPLC led to the isolation of the new compounds 1 and 2 belonging to the spirotetronate class of polyketides. Their structures were determined using a combination of HRMS, 1D and 2D NMR experiments and comparison with the spectra reported for phocoenamicin. Antibacterial activity tests of the pure compounds against these pathogens revealed minimal inhibitory concentration (MIC) values ranging from4 to 64 mu g/mL forMRSA, and 16 to 32 mu g/mL forM. tuberculosis H37Ra, with no significant activity found against M. bovis and vancomycin-resistant Enterococcus faecium (VRE) at concentrations below 128 mu g/mL, and weak activity detected against Bacillus subtilis grown on agar plates.
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