Article
Medicine, General & Internal
Junhua Zhao, Zhaojun Han, Chang Xu, Lu Li, Haimeng Pei, Yongxi Song, Zhenning Wang, Bo Tang
Summary: A microfluidic device was developed for rapid and high-throughput separation of cancer cells from ascites and peritoneal lavages, enabling the diagnosis of gastric cancer and the analysis of ascites cancer cells at the single-cell level. This technology improves the diagnosis of peritoneal metastasis and investigation of therapeutic targets.
Review
Chemistry, Analytical
Jianyu Shi, Yating Pan, Xudong Liu, Wenjian Cao, Ying Mu, Qiangyuan Zhu
Summary: Spatial profiling technologies have promising application prospects in filling the gap left by the loss of spatial information in traditional single-cell sequencing. Within a few years of development, these technologies have made significant progress in resolution and simplicity. This review discusses the development of spatial omics sequencing based on microfluidic array chips, barcoding strategies using various microfluidic designs, and the pros and cons of each strategy. Commercialized solutions for spatial profiling and future research directions are also presented.
Article
Chemistry, Analytical
Weihua Fan, Qiao Xiong, Yuqing Ge, Ting Liu, Shuxiong Zeng, Jianlong Zhao
Summary: This work presents a novel method for distinguishing the grade of bladder cancer at a single-cell level, using microdroplets and microelectrodes to measure the impedance of individual cells in a label-free and non-invasive manner. The findings suggest that this impedance analysis device based on droplet microfluidics holds promise in clinical and point-of-care diagnostics.
Review
Chemistry, Analytical
Soojung Kim, Hyerin Song, Heesang Ahn, Taeyeon Kim, Jihyun Jung, Soo Kyung Cho, Dong-Myeong Shin, Jong-ryul Choi, Yoon-Hwae Hwang, Kyujung Kim
Summary: Electrical impedance biosensors combined with microfluidic devices offer high-throughput analysis of biological processes at the single-cell scale, enabling sensitive determination of drug effectiveness and toxicity. The efficiency and performance of the sensors are ultimately determined by the methods of single-cell trapping, while identifying the latest trends opens up opportunities for technological advancement. This leading technology in cell biology, pathology, and pharmacology allows for further understanding of complex cellular functions and mechanisms through precise analysis capabilities.
Article
Biochemical Research Methods
Perizat Kanabekova, Bereke Dauletkanov, Zhibek Bekezhankyzy, Sultanali Toktarkan, Alma Martin, Tri T. Pham, Konstantinos Kostas, Gulsim Kulsharova
Summary: Here, we present a fluorescent electrospun nanofiber membrane that can be integrated into microfluidic devices for lung-on-a-chip applications. We developed a hybrid PCL-collagen membrane and tested its biocompatibility for mimicking a basement membrane. The membrane, with a thickness of approximately 10 μm, can be adjusted for porosity and offers excellent biocompatibility. Several membrane variations were evaluated using various characterization techniques, and a microfluidic chip was built and integrated with the membrane for cell culture and biocompatibility studies. The integration of this novel membrane into microfluidic devices can enhance the recapitulation of the lung microenvironment in laboratory settings.
Article
Chemistry, Analytical
Valber A. Pedrosa, Kangfu Chen, Thomas J. George, Z. Hugh Fan
Summary: Liquid biopsy has been developed for diagnosing and monitoring cancer. However, there is still room for improvement in capturing circulating tumor cells (CTCs). In this study, we integrated gold nanoparticles with a microfluidic platform to develop an efficient CTC detection system, which achieved CTC capture within 120 min. Our method was optimized and demonstrated high efficiency (90% ± 3.2%) in capturing CTCs from pancreatic tumor cell lines and patients with metastatic pancreatic cancer.
Article
Chemistry, Analytical
Tomasz R. Szymborski, Marta Czaplicka, Ariadna B. Nowicka, Joanna Trzcinska-Danielewicz, Agnieszka Girstun, Agnieszka Kaminska
Summary: This study demonstrates a unique arrangement of surface-enhanced Raman spectroscopy (SERS) technique and dielectrophoretic effect for the detection of freely circulating cancer cells (CTCs). The combination of SERS and dielectrophoresis allows for efficient deposition of CTCs on the SERS platform. The developed approach shows a limit of detection (LOD) of 20 cells/mL, highlighting its clinical potential and usefulness.
Review
Biotechnology & Applied Microbiology
Sasan Jalili-Firoozinezhad, Claudia C. Miranda, Joaquim M. S. Cabral
Summary: Organ-on-chip culture technology is a promising tool for studying tissue development and homeostasis, bringing us closer to conducting human experimentation in vitro. By linking on-chip models of multiple organs, a functional human body-on-chip platform can be created, with advantages in disease modeling, therapeutic development, and personalized medicine. Current limitations of the body-on-chip approach are discussed, along with new ideas to address pending issues.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Computer Science, Artificial Intelligence
Zhonghao Zhang, Qiqiang Li, Wen Song, Pengfei Wei, Jing Guo
Summary: In this paper, a novel automatic cell segment method based on concave point detection and matching is proposed. By using a deep neural network and high-quality image deblurring techniques, the method can accurately extract cell contours and address the challenges of segmenting touching cells by detecting and matching concave points.
EXPERT SYSTEMS WITH APPLICATIONS
(2022)
Article
Multidisciplinary Sciences
Xiaocheng Liu, Hanliang Zhu, Jan Sabo, Zdenek Lansky, Pavel Neuzil
Summary: The study demonstrates that using a silicon-on-insulator (SOI) substrate can rectify the issue of uneven surfaces affecting light penetration in microfluidic systems, thereby improving the signal-to-noise ratio and fluorescence image quality compared to conventional silicon wafers.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Gyula Kajner, Albert Keri, Adam Belteki, Sandor Valkai, Andras Der, Zsolt Geretovszky, Gabor Galbacs
Summary: This study explored the potential of online sample pretreatment steps on microfluidic chips for single particle ICP-MS measurements. By optimizing the design and functions of the chips, efficient analysis of nanoparticles with high efficiency and precision was achieved.
Article
Engineering, Environmental
Soo Chung, Andrew Loh, Christian M. Jennings, Katelyn Sosnowski, Sung Yong Ha, Un Hyuk Yim, Jeong-Yeol Yoon
Summary: We developed a novel method to screen different types of oil using a wax-printed paper-based microfluidic platform. The oil samples flowed through micrometer-scale channels via capillary action, while their components were filtered and separated. The fluctuation in the capillary flow velocity profiles was utilized to classify oil types. A Raspberry Pi camera captured video clips, which were analyzed using a custom Python code to obtain the capillary flow velocity profiles. 106 velocity profiles were recorded from various oil samples, and principal component analysis, support vector machine, and linear discriminant analysis were used to classify the oil types with high accuracy.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Simon Raffy, Etienne Palleau, Brice Calvignac, Guillaume Brotons, Guillaume Lefebvre, Nicolas Rolley, Sebastien Teychene, Bernard Viguier, Sara Castano Cerezo, Gilles Truan, Laurence Ressier
Summary: The choice of materials in chip fabrication for microfluidics is crucial, and this study proposes a versatile approach using a combination of epoxy materials to fabricate microfluidic chips with various potentialities. The chips demonstrate good chemical resistance, optical transparency, biocompatibility, high pressure capability, and integration of high aspect ratio channels, making them promising for prototyping in a wide range of applications and fundamental studies.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Manuel Luitz, Buesra M. Kirpat Konak, Ahmad Sherbaz, Richard Prediger, Niloofar Nekoonam, Barbara Di Ventura, Frederik Kotz-Helmer, Bastian E. Rapp
Summary: Two-photon lithography is an advanced additive manufacturing technique that can produce high-resolution objects with micro-scale features. In this study, a process flow was developed to fabricate embedded microfluidic chips with nanoscale channels, and a method for washing the channels effectively was introduced. This technique allows for the rapid production of different types of microfluidic chips, reducing the time from design to manufacturing.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Physical
Honggang Wang, Huixiang Wang, Zhen Tian, Hao Zhang, Yafeng Huang, Xianbo Qiu, Duli Yu, Lulu Zhang
Summary: The study proposes a simulation method based on step function to investigate molecular binding and dissociation processes, coupled with surface plasmon wave evanescent field. Experimental verification on human serum albumin was conducted to study the effects of various conditions on molecular dynamics binding characteristics. The optimized parameters obtained could improve experimental detection accuracy and reduce reaction reagents, making the established simulation and experimental methods a powerful tool for future molecular interaction analysis.