Article
Immunology
Gregory J. Wilson, Benigno Rodriguez, Shuying Sue Li, Mary Allen, Ian Frank, Erika Rudnicki, Meg Trahey, Spyros Kalams, Drew Hannaman, David K. Clarke, Rong Xu, Michael Egan, John Eldridge, Michael Pensiero, Theresa Latham, Guido Ferrari, David C. Montefiori, Georgia D. Tomaras, Stephen C. De Rosa, Jeffrey M. Jacobson, Maurine D. Miner, Marnie Elizaga
Summary: This study evaluated the safety and immunogenicity of a DNA vaccine prime followed by a viral vector boost for HIV subtypes B and C. The results showed that the vaccine regimen was safe and tolerable, and induced immune responses to specific HIV antigens.
Article
Immunology
Christopher A. Gonelli, Hannah A. D. King, Charlene Mackenzie, Secondo Sonza, Rob J. Center, Damian F. J. Purcell
Summary: This study optimized the incorporation of Env into mVLPs and stabilized Env on the VLP surface, leading to efficient presentation of neutralizing antibody epitopes and broader range of Env-specific antibody isotypes. mVLPs bearing HA-TMCT-modified Env consistently induced modest neutralization activity. These mVLPs are potentially useful immunogens for eliciting neutralizing antibody responses targeting native Env epitopes on infectious HIV-1 virions.
Article
Virology
Zhi Yang, Kim-Marie A. Dam, Jonathan M. Gershoni, Susan Zolla-Pazner, Pamela J. Bjorkman
Summary: The RV144 HIV-1 clinical vaccination trial showed modest protection against viral infection. Antibody responses to the V1V2 region of HIV-1 Env gp120 were correlated inversely with the risk of infection, and data from three other clinical vaccine trials suggested a similar signal. In this study, V2i antibodies and CD4i antibody CG10 were characterized for their interactions with open Env trimers bound to CD4. The findings provide insights into the immune mechanisms during HIV-1 infection.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Ana Gradissimo, Viswanathan Shankar, Fanua Wiek, Lauren St. Peter, Yevgeniy Studentsov, Anne Nucci-Sack, Angela Diaz, Sarah Pickering, Nicolas F. Schlecht, Robert D. Burk
Summary: The study aimed to investigate the serological levels of antibodies against HPV16 prior to incident infections, finding that high-risk controls had higher antibody levels compared to incident cases and randomly selected controls after completing the three doses of the 4vHPV vaccine.
Article
Medicine, Research & Experimental
Yoann Aldon, Paul F. McKay, Jorge Moreno Herrero, Annette B. Vogel, Reka Levai, Pauline Maisonnasse, Nathalie Dereuddre-Bosquet, Heinrich Haas, Katalin Fabian, Roger Le Grand, Ugur Sahin, Robin J. Shattock
Summary: saRNA platform, particularly VEEV saRNA, shows potential for high protein expression and strong immune responses in delivering stabilized HIV-1 Env. Membrane-bound Env design leads to faster immune kinetics and better IgG responses, with promise for diverse vaccine regimens.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Immunology
Eleonora Melzi, Jordan R. Willis, Krystal M. Ma, Ying-Cing Lin, Sven Kratochvil, Zachary T. Berndsen, Elise A. Landais, Oleksandr Kalyuzhniy, Usha Nair, John Warner, Jon M. Steichen, Anton Kalyuzhniy, Amber Le, Simone Pecetta, Manfredo Perez, Kathrin Kirsch, Stephanie R. Weldon, Samantha Falcone, Sunny Himansu, Andrea Carfi, Devin Sok, Andrew B. Ward, William R. Schief, Facundo D. Batista
Summary: Eliciting broadly neutralizing antibodies is essential for HIV vaccine design, and research has shown that high-affinity ApexGT immunogens are crucial for activating and selecting mature bnAb mutations. Additionally, mRNA-LNP is a suitable approach for significantly improving B cell responses.
Article
Immunology
Suzanne Day, Charandeep Kaur, Hannah M. Cheeseman, Emily de Groot, Leon R. McFarlane, Maniola Tanaka, Sofia Coelho, Tom Cole, Nana-Marie Lemm, Adrian Lim, Rogier W. Sanders, Becca Asquith, Robin J. Shattock, Katrina M. Pollock
Summary: This study reveals the importance of rational antigen design and harnessing CD4+ T cell help in the lymph nodes for the formation of broadly neutralizing antibodies (bNAbs) during experimental HIV immunization. The lymph node cells (LNC) were found to be phenotypically and functionally distinct from peripheral blood mononuclear cells (PBMC), indicating that blood is only a limited proxy for the T cell lymphatic response to immunization. Additionally, HIV-specific CD4+ T cells in the lymph node can be rapidly induced upon experimental injection with HIV immunogens.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Edurne Rujas, Beatriz Apellaniz, Johana Torralba, David Andreu, Jose M. M. Caaveiro, Shixia Wang, Shan Lu, Jose L. Nieva
Summary: This study investigates the role of fusion peptide and Trp-rich membrane proximal external region in HIV-1 fusion and demonstrates that liposome-based formulations containing FP-MPER hybrid peptides can induce the production of specific neutralizing antibodies in rabbits. The results support the use of liposomes as vaccine carriers and the inclusion of lipid membranes in immunogens to elicit specific humoral responses.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Immunology
Julia Strandmark, Alansana Darboe, Joann Diray-Arce, Rym Ben-Othman, Sofia M. Vignolo, Shun Rao, Kinga K. Smolen, Geert Leroux-Roels, Olubukola T. Idoko, Guzman Sanchez-Schmitz, Al Ozonoff, Ofer Levy, Tobias R. Kollmann, Arnaud Marchant, Beate Kampmann, EPIC Consortium
Summary: The study shows that a single birth dose of Hepatitis B vaccine can induce antigen-specific polyfunctional CD4(+) T-cells, and the frequency of polyfunctional T-cells increases after completing the vaccine schedule. The production of these polyfunctional T-cells is positively correlated with serum antibody titres.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Daniel P. Leaman, Armando Stano, Yajing Chen, Lei Zhang, Michael B. Zwick
Summary: The study introduces a novel multivalent mEnv vaccine platform called MELs, which does not require protein engineering or extraneous proteins. Through a sequential immunization scheme in rabbits, MELs were able to elicit antibodies that neutralized tier 2 HIV isolates.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Clara Bessen, Carlos Plaza-Sirvent, Agit Simsek, Jaydeep Bhat, Corinna Marheinecke, Doris Urlaub, Petra Bonowitz, Sandra Busse, Sabrina Schumann, Elena Vidal Blanco, Adriane Skaletz-Rorowski, Norbert H. Brockmeyer, Oliver Overheu, Anke Reinacher-Schick, Simon Faissner, Carsten Watzl, Stephanie Pfaender, Anja Potthoff, Ingo Schmitz
Summary: The antibody responses to SARS-CoV-2 vaccines in people living with HIV are significantly reduced compared to HIV-negative individuals, while CD4+ T cell responses are comparable. This suggests that low CD4+ T cell counts do not necessarily interfere with cellular immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Can M. Sungur, Qiankun Wang, Ayse N. Ozanturk, Hongbo Gao, Aaron J. Schmitz, Marina Cella, Wayne M. Yokoyama, Liang Shan
Summary: The role of NK cells in HIV-1 infections was investigated using a humanized mouse model. The study showed that NK cells directly provided anti-HIV-1 responses in nonlymphoid organs, but had limited functionality in lymphoid organs. Antiretroviral therapy and a broadly neutralizing antibody, PGT121, were found to enhance NK cell function and reduce viral load.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Cell Biology
Michelle K. Ash, Pavan P. Bhimalli, Byoung-Kyu Cho, Basil Baby Mattamana, Stephanie Gambut, Imad Tarhoni, Cristina L. Fhied, Anjelica F. Reyes, Samantha J. Welninski, Jaison Arivalagan, Fernanda Negrao, Renu Goel, Todd L. Beck, Thomas J. Hope, Beverly E. Sha, Young Ah Goo, Lena Al-Harthi, Joao I. Mamede, Jeffrey A. Borgia, Neil L. Kelleher, Jeffrey R. Schneider
Summary: This study investigated the impact of SARS-CoV-2 infection and vaccination on the immune response by measuring changes in IgG glycosylation. The results showed that hospitalized COVID-19 patients had elevated levels of inflammatory glycans, while mild patients had increased levels of anti-inflammatory glycans. Vaccinated individuals with low RBD antibody levels and low neutralization showed similar IgG glycan traits as hospitalized COVID-19 patients.
Article
Immunology
Ros Chapman, Michiel van Diepen, Nicola Douglass, Shireen Galant, Mohamed Jaffer, Emmanuel Margolin, Phindile Ximba, Tandile Hermanus, Penny L. Moore, Anna-Lise Williamson
Summary: The study compared a recombinant LSDV expression system to an equivalent recombinant modified vaccinia Ankara (MVA) system for eliciting humoral immune responses against HIV-1 Env. Different vaccination regimens using LSDVGC5 induced high titers of neutralizing antibodies, with some regimens leading to even higher levels of neutralizing antibodies.
Article
Multidisciplinary Sciences
Fengyin Li, Xin Zhao, Yali Zhang, Peng Shao, Xiaoke Ma, William J. Paradee, Chengyu Liu, Jianmin Wang, Hai-Hui Xue
Summary: Precise regulation of coinhibitory receptors is crucial for maintaining immune tolerance and protective immunity. In protein immunization, T-FH cells lacking Tcf1 and Lef1 show abnormal upregulation of CTLA4 and LAG3, impacting B-cell help. Targeting CTLA4 and LAG3 can rectify T-FH cell defects and balance immune responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Correction
Virology
Hassan Elsayed, Ghulam Nabi, William J. McKinstry, Keith K. Khoo, Johnson Mak, Andres M. Salazar, Matthias Tenbusch, Vladimir Temchura, Klaus Ueberla
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Orian Gilmer, Elodie Mailler, Jean-Christophe Paillart, Assia Mouhand, Carine Tisne, Johnson Mak, Redmond P. Smyth, Roland Marquet, Valerie Vivet-Boudou
Summary: Maturation of HIV-1 viral particles is essential for infectivity. While the structural rearrangements of viral proteins during maturation are well-known, the maturation of genomic RNA (gRNA) structure is less explored. This study investigates the interactions between Pr55(Gag) or its maturation products and the gRNA region, revealing their distinct contributions to gRNA structural maturation.
Article
Microbiology
Christopher J. Day, Rachael L. Hardison, Belinda L. Spillings, Jessica Poole, Joseph A. Jurcisek, Johnson Mak, Michael P. Jennings, Jennifer L. Edwards
Summary: This study found that HIV-1 can bind to the CR3 protein on the surface of cervical epithelial cells with high affinity, and can transcytose across the cervical epithelial cell layer through a CR3-dependent mechanism. This suggests a unique role for CR3 on mucosal epithelial cells in facilitating HIV-1 attachment and entry. The HIV-CR3 interaction may be an efficient pathway for HIV delivery to subepithelial lymphocytes following virus transmission across an intact cervical epithelial barrier. Strategies to prevent transmission via this pathway are presented.
Article
Cell Biology
Belinda L. Spillings, Christopher J. Day, Albert Garcia-Minambres, Anupriya Aggarwal, Nicholas D. Condon, Thomas Haselhorst, Damian F. J. Purcell, Stuart G. Turville, Jennifer L. Stow, Michael P. Jennings, Johnson Mak
Summary: This study reveals that HIV is captured near the lymphocyte surface in a virion-glycan-dependent manner. Removing certain glycans impairs virus-cell binding and interfering with glycan interactions reduces HIV infectivity. These findings provide new insights into the interaction between HIV and host cells.
Article
Engineering, Biomedical
D. Damm, K. Kostka, C. Weingartner, J. T. Wagner, L. Rojas-Sanchez, S. Gensberger-Reigl, V. Sokolova, K. Uberla, M. Epple, V. Temchura
Summary: This study focuses on a selective bioconjugation method to link Env trimers to the surface of medically relevant calcium phosphate nanoparticles, maintaining protein conformation and having broad potential applications in vaccination and diagnostic approaches.
ACTA BIOMATERIALIA
(2022)
Article
Environmental Sciences
Noureddine Ouerfelli, Narcisa Vrinceanu, Diana Coman, Adriana Lavinia Cioca
Summary: This report develops a mathematical model for studying the transmission and control of COVID-19 in Romania, aiming to emphasize the impact of control measures and case detection rate on disease spread to guide decision-making.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Biophysics
Anita Mouttou, Erwan Bremaud, Julien Noero, Rayane Dibsy, Coline Arone, Johnson Mak, Delphine Muriaux, Hugues Berry, Cyril Favard
Summary: Many transient processes in cells result from the binding of cytosolic proteins to membranes. Quantifying this membrane binding and its associated diffusion is crucial for understanding cellular dynamics. This study combines computer simulations and experimental techniques to propose a new approach for measuring membrane binding using fluorescence correlation spectroscopy (FCS). The results show that this approach enables accurate estimation of membrane partitioning and diffusion properties for peripheral membrane molecules.
BIOPHYSICAL JOURNAL
(2023)
Letter
Virology
Peter Speck, Jason Mackenzie, Rowena A. Bull, Barry Slobedman, Heidi Drummer, Johanna Fraser, Lara Herrero, Karla Helbig, Sarah Londrigan, Gregory Moseley, Natalie Prow, Grant Hansman, Robert Edwards, Chantelle Ahlenstiel, Allison Abendroth, David Tscharke, Jody Hobson-Peters, Robson Kriiger-Loterio, Rhys Parry, Glenn Marsh, Emma Harding, David A. Jacques, Matthew J. Gartner, Wen Shi Lee, Julie McAuley, Paola Vaz, Frank Sainsbury, Michelle D. Tate, Jane Sinclair, Allison Imrie, Stephen Rawlinson, Andrew Harman, Jillian M. Carr, Ebony A. Monson, Merilyn Hibma, Timothy J. Mahony, Thomas Tu, Robert J. Center, Lok Bahadur Shrestha, Robyn Hall, Morgyn Warner, Vernon Ward, Danielle E. Anderson, Nicholas S. Eyre, Natalie E. Netzler, Alison J. Peel, Peter Revill, Michael Beard, Alistair R. Legione, Alexandra J. Spencer, Adi Idris, Jade Forwood, Subir Sarker, Damian F. J. Purcell, Nathan Bartlett, Joshua M. Deerain, Bruce J. Brew, Sassan Asgari, Helen Farrell, Alexander Khromykh, Daniel Enosi Tuipulotu, David Anderson, Sevim Mese, Yaman Tayyar, Kathryn Edenborough, Jasim Muhammad Uddin, Abrar Hussain, Connor J. I. Daymond, Jacinta Agius, Karyn N. Johnson, Paniz Shirmast, Mahdi Abedinzadeshahri, Robin MacDiarmid, Caroline L. Ashley, Jay Laws, Lucy L. Furfaro, Thomas D. Burton, Stephen M. R. Johnson, Zahra Telikani, Mary Petrone, Justin A. Roby, Carolyn Samer, Andreas Suhrbier, April van der Kamp, Anthony Cunningham, Celeste Donato, Jackie Mahar, Wesley D. Black, Subhash Vasudevan, Roman Lenchine, Kirsten Spann, Daniel J. Rawle, Penny Rudd, Jessica Neil, Richard Kingston, Timothy P. Newsome, Ki Wook Kim, Johnson Mak, Kym Lowry, Nathan Bryant, Joanne Meers, Jason A. Roberts, Nigel McMillan, Larisa I. Labzin, Andrii Slonchak, Leon E. Hugo, Bennett Henzeler, Natalee D. Newton, Cassandra T. David, Patrick C. Reading, Camille Esneau, Tatiana Briody, Najla Nasr, Donna McNeale, Brian McSharry, Omid Fakhri, Bethany A. Horsburgh, Grant Logan, Paul Howley, Paul Young
JOURNAL OF VIROLOGY
(2023)
Editorial Material
Biochemical Research Methods
Julia C. Kenyon, Johnson Mak
Summary: Nano-DMS-MaP examines the structures of individual RNA isoforms, allowing for direct assessment of the structural diversity of different RNAs within cells.
Letter
Microbiology
Peter Speck, Jason Mackenzie, Rowena A. Bull, Barry Slobedman, Heidi Drummer, Johanna Fraser, Lara Herrero, Karla Helbig, Sarah Londrigan, Gregory Moseley, Natalie Prow, Grant Hansman, Robert Edwards, Chantelle Ahlenstiel, Allison Abendroth, David Tscharke, Jody Hobson-Peters, Robson Kriiger-Loterio, Rhys Parry, Glenn Marsh, Emma Harding, David A. Jacques, Matthew J. Gartner, Wen Shi Lee, Julie McAuley, Paola Vaz, Frank Sainsbury, Michelle D. Tate, Jane Sinclair, Allison Imrie, Stephen Rawlinson, Andrew Harman, Jillian M. Carr, Ebony A. Monson, Merilyn Hibma, Timothy J. Mahony, Thomas Tu, Robert J. Center, Lok Bahadur Shrestha, Robyn Hall, Morgyn Warner, Vernon Ward, Danielle E. Anderson, Nicholas S. Eyre, Natalie E. Netzler, Alison J. Peel, Peter Revill, Michael Beard, Alistair R. Legione, Alexandra J. Spencer, Adi Idris, Jade Forwood, Subir Sarker, Damian F. J. Purcell, Nathan Bartlett, Joshua M. Deerain, Bruce J. Brew, Sassan Asgari, Helen Farrell, Alexander Khromykh, Daniel Enosi Tuipulotu, David Anderson, Sevim Mese, Yaman Tayyar, Kathryn Edenborough, Jasim Muhammad Uddin, Abrar Hussain, Connor J. Daymond, Jacinta Agius, Karyn N. Johnson, Paniz Shirmast, Mahdi Abedinzadeshahri, Robin MacDiarmid, Caroline L. Ashley, Jay Laws, Lucy L. Furfaro, Thomas D. Burton, Stephen M. R. Johnson, Zahra Telikani, Mary Petrone, Justin A. Roby, Carolyn Samer, Andreas Suhrbier, April van der Kamp, Anthony Cunningham, Celeste Donato, Jackie Mahar, Wesley D. Black, Subhash Vasudevan, Roman Lenchine, Kirsten Spann, Daniel J. Rawle, Penny Rudd, Jessica Neil, Richard Kingston, Timothy P. Newsome, Ki Wook Kim, Johnson Mak, Kym Lowry, Nathan Bryant, Joanne Meers, Jason A. Roberts, Nigel McMillan, Larisa I. Labzin, Andrii Slonchak, Leon E. Hugo, Bennett Henzeler, Natalee D. Newton, Cassandra T. David, Patrick C. Reading, Camille Esneau, Tatiana Briody, Najla Nasr, Donna McNeale, Brian McSharry, Omid Fakhri, Bethany A. Horsburgh, Grant Logan, Paul Howley, Paul Young
Letter
Microbiology
Peter Speck, Jason Mackenzie, Rowena A. Bull, Barry Slobedman, Heidi Drummer, Johanna Fraser, Lara Herrero, Karla Helbig, Sarah Londrigan, Gregory Moseley, Natalie Prow, Grant Hansman, Robert Edwards, Chantelle Ahlenstiel, Allison Abendroth, David Tscharke, Jody Hobson-Peters, Robson Kriiger-Loterio, Rhys Parry, Glenn Marsh, Emma Harding, David A. Jacques, Matthew J. Gartner, Wen Shi Lee, Julie McAuley, Paola Vaz, Frank Sainsbury, Michelle D. Tate, Jane Sinclair, Allison Imrie, Stephen Rawlinson, Andrew Harman, Jillian M. Carr, Ebony A. Monson, Merilyn Hibma, Timothy J. Mahony, Thomas Tu, Robert J. Center, Lok Bahadur Shrestha, Robyn Hall, Morgy Warner, Vernon Ward, Danielle E. Anderson, Nicholas S. Eyre, Natalie E. Netzler, Alison J. Peel, Peter Revill, Michael Beard, Alistair R. Legione, Alexandra J. Spencer, Adi Idris, Jade Forwood, Subir Sarker, Damian F. J. Purcell, Nathan Bartlett, Joshua M. Deerain, Bruce J. Brew, Sassan Asgari, Helen Farrell, Alexander Khromykh, Daniel Enosi Tuipulotu, David Anderson, Sevim Mese, Yaman Tayyar, Kathryn Edenborough, Jasim Muhammad Uddin, Abrar Hussain, Connor J. I. Daymond, Jacinta Agius, Karyn N. Johnson, Paniz Shirmast, Mahdi Abedinzadeshahri, Robin MacDiarmid, Caroline L. Ashley, Jay Laws, Lucy L. Furfaro, Thomas D. Burton, Stephen M. R. Johnson, Zahra Telikani, Mary Petrone, Justin A. Roby, Carolyn Samer, Andreas Suhrbier, April van der Kamp, Anthony Cunningham, Celeste Donato, Jackie Mahar, Wesley D. Black, Subhash Vasudevan, Roman Lenchine, Kirsten Spann, Daniel J. Rawle, Penny Rudd, Jessica Neil, Richard Kingston, Timothy P. Newsome, Ki Wook Kim, Johnson Mak, Kym Lowry, Nathan Bryant, Joanne Meers, Jason A. Roberts, Nigel McMillan, Larisa I. Labzin, Andrii Slonchak, Leon E. Hugo, Bennett Henzeler, Natalee D. Newton, Cassandra T. David, Patrick C. Reading, Camille Esneau, Tatiana Briody, Najla Nasr, Donna McNeale, Brian McSharry, Omid Fakhri, Bethany A. Horsburgh, Grant Logan, Paul Howley, Paul Young
Article
Multidisciplinary Sciences
Rayane Dibsy, Erwan Bremaud, Johnson Mak, Cyril Favard, Delphine Muriaux
Summary: This study characterizes the role of cortical actin, viral Gag protein, and host factor Arpin in the assembly and release of HIV-1 from infected T lymphocytes.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Chandan Kishor, Belinda L. Spillings, Johana Luhur, Corinne A. Lutomski, Chi-Hung Lin, William J. McKinstry, Christopher J. Day, Michael P. Jennings, Martin F. Jarrold, Johnson Mak
Summary: In this study, the authors use HIV as a model system to investigate the mechanism of directional protein delivery. They find that the viral assembly machinery Gag is copolarized with the intracellular calcium gradient and binds specifically with Ca2+. Mutation of Ca2+ binding amino acids affects protein trafficking and virion release.
Article
Microbiology
Christopher J. Day, Rachael L. Hardison, Belinda L. Spillings, Jessica Poole, Joseph A. Jurcisek, Johnson Mak, Michael P. Jennings, Jennifer L. Edwards
Summary: The study shows that HIV-1 binds to CR3 with high affinity, which is necessary and sufficient for HIV adherence to, and transcytosis across, polarized, human primary cervical epithelial cells. This suggests a unique role for CR3 on epithelial cells in facilitating HIV-1 attachment and entry. Strategies targeting the HIV-CR3 interaction may prevent transmission via this efficient pathway.